BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,234	C→A	5.4%	M47I (ATG→ATT)	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,234	0	C	A	5.4%	1550.4 / 13.6	641	M47I (ATG→ATT)	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (402/177); new base A (16/43); total (420/221)
Fisher's exact test for biased strand distribution p-value = 4.05e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 642 total aligned reads displayed.

CTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTC  >  NZ_CP034943/3851095‑3851371

      tGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                      <  2:4424338/140‑1 (MQ=255)

      tGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                      <  2:5004248/140‑1 (MQ=255)

        tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAACTGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                    >  2:7441186/1‑140 (MQ=255)

        tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                    >  1:104262/1‑140 (MQ=255)

        tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                    >  2:4221701/1‑140 (MQ=255)

        tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                    >  1:457259/1‑140 (MQ=255)

        tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                    >  1:3692425/1‑140 (MQ=255)

        tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                    >  1:3205760/1‑140 (MQ=255)

        tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                    >  1:2524235/1‑140 (MQ=255)

        tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGCTTCg                                                                                                                                    >  1:6370556/1‑140 (MQ=255)

        acccAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                    >  2:6535058/2‑140 (MQ=255)

          ccAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGAt                                                                                                                                  <  1:3342635/140‑1 (MQ=255)

                  ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGTACCAGTCGGTATTACCTGTATCATGATTAGATCGTATTCa                                                                                                                          >  1:1183459/1‑140 (MQ=255)

                  ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGcc                                                                                                                          >  1:507517/1‑139 (MQ=255)

                  ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                          >  1:3014570/1‑140 (MQ=255)

                  ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                          >  1:5903697/1‑140 (MQ=255)

                  ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                          >  1:3885065/1‑140 (MQ=255)

                  ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                          >  2:4846395/1‑140 (MQ=255)

                  ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATGCGATCGTATGCa                                                                                                                          >  1:6142145/1‑140 (MQ=255)

                    ctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCCTGATTCGCTCGTATGCAAg                                                                                                                        >  2:7271029/1‑140 (MQ=255)

                     tctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGc                                                                                                                           <  1:5366205/136‑1 (MQ=255)

                       tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTACGCATAACCTTCACAGTACTCGGACAGGTCTTCTATCAATGAACCAGTCGTTCGTACCTGTATCAGGTGTCGATCGTATGCAAGccc                                                                                                                     >  1:4451794/1‑140 (MQ=255)

                       tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                     >  2:3974024/1‑140 (MQ=255)

                            tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATATCCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGGTCTTACCTGTATCAGTAGTCGTTCGGATGCAAGCCCGTccc                                                                                                                >  1:7511670/1‑140 (MQ=255)

                            tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTTTTACCTGTATGATTAGTCGATCGGATGCAAGCCCTGccc                                                                                                                >  2:2268883/1‑140 (MQ=255)

                            tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTccc                                                                                                                >  1:1374502/1‑140 (MQ=255)

                            tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGCAACCCCTTccc                                                                                                                >  2:111195/1‑140 (MQ=255)

                            tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGCAACACCTTccc                                                                                                                >  2:3879395/1‑140 (MQ=255)

                            tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCGTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGGTATTACCTGTATCATGATTCGATCGTATGCAACCCCATccc                                                                                                                >  2:4603773/1‑140 (MQ=255)

                              tGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTACTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTACC‑aa                                                                                                             >  2:6809856/1‑140 (MQ=255)

                               ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaa                                                                                                            >  2:3667961/1‑140 (MQ=255)

                               ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaa                                                                                                            >  2:1658210/1‑140 (MQ=255)

                               ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaa                                                                                                            <  1:3108474/140‑1 (MQ=255)

                               ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaa                                                                                                            <  1:7067730/140‑1 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATTAACCAGTCGTTATTACCTGTATAATGATTCGATCGTATTCAAGCCCTTACC‑aaaaa                                                                                                          >  2:2823606/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGACCCAGTCGTTATTACCTGTCTCATGATTCGAGCGTATGCGCGCCCTTCCC‑aaata                                                                                                          >  1:6530820/1‑138 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCGGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                          >  2:1497651/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTCTTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                          >  1:2133229/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGCTCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                          >  1:4718159/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCGC‑aaaaa                                                                                                          >  2:4274459/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                          >  1:2282336/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                          >  1:6662041/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                          >  1:73737/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                          >  1:3507613/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                          >  1:1981673/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                          >  1:911318/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                          >  2:2703029/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                          >  1:5454167/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCGTCAC‑aaaaa                                                                                                          >  1:4226746/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGAAAGCCCTTCCC‑aaaaa                                                                                                          >  2:4079498/1‑140 (MQ=255)

                                   tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATTCAAGCCCTTCCC‑AAAAAct                                                                                                        >  1:7274109/1‑139 (MQ=255)

                                          ggAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCACGCCCTTACC‑AAAAACGATAAAAc                                                                                                 >  2:6439860/1‑140 (MQ=255)

                                          ggAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAAc                                                                                                 >  2:5423325/1‑140 (MQ=255)

                                              ttatctTTTCGATATTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGCACTGGTATTCTATAAATGAACCAGTGGTTATTACGTGTATCATTTTTCGATCGTATGCAAGCCCTGCCC‑AAAAACGATAACACGAct                                                                                             <  2:7534186/137‑1 (MQ=255)

                                                  ctTTTCGATTTTTTTATCACCTTGTAAGCATAACCTTAAAATTACTCGGACCGGTCTTCTATAAATGACCCAGTCTTTATTACCTGTATCATGTTTCGATGGTATGCACGCCCTCCCA‑AAAAACCATAACACGACTCTGc                                                                                         <  2:3585518/140‑1 (MQ=255)

                                                      tCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACtcttcttct                                                                                     >  2:5307965/1‑140 (MQ=255)

                                                      tCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGcttct                                                                                     >  2:89929/1‑140 (MQ=255)

                                                                      cTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACACTGCTTATTAAACTTCGCAAGACa                                                                     >  2:1286494/1‑140 (MQ=255)

                                                                       ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATTAACCAGTCGTTATTACCTGTATCATGAATAGATCGTCTGCAAGACCTTCCC‑AAAAACGATAAAACTAATCTGCATCTTCAATTTCGCAAGACAt                                                                    >  2:4551880/1‑140 (MQ=255)

                                                                                tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAATCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                           >  1:5592891/1‑140 (MQ=255)

                                                                                tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACttt                                                           >  2:7096638/1‑139 (MQ=255)

                                                                                tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                           >  2:6998345/1‑140 (MQ=255)

                                                                                tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                           >  2:3364661/1‑140 (MQ=255)

                                                                                tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                           >  2:3776599/1‑140 (MQ=255)

                                                                                tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAAATtg                                                           >  1:4629551/1‑140 (MQ=255)

                                                                                tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAAAGACTCTGCTTATTCAAGTTCGCAAGACATAACAACttt                                                           >  1:4067111/1‑139 (MQ=255)

                                                                                   ccTTCAAAGTACTCCTACAGTTATTCTATAAAACAACCCGTCGTTATTCCGTTTATCTTGTTTCGATCGTTTGCAAGCCTTACAC‑ACAAACGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                        <  2:2389012/140‑1 (MQ=255)

                                                                                   ccTTCAAAGTACCCGGACATGTGTTATATAAATTAACCCGTCGTTATTACCTTTATAATTATTCGATCGTATGCAAGCCCTTCCA‑AAAAACGAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                        <  2:6674489/140‑1 (MQ=255)

                                                                                   ccTCCAAAGTACTCGCACCGGTCTTATATAAAGACACCAGTCGTTATTCCCTGTATCATGTTGCGAGCGTATGCCACCCCTTCCC‑AAAAACCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                        <  2:6044106/140‑1 (MQ=255)

                                                                                   cataaaaaGTCCTCGGACCGGTAGTATATAATTTAACCAGTCGTTATTACGTGTATCATGTTGCGATCGTATGCAAGCCCGACCC‑AAAACCGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACGggatg                                                        <  2:1776063/135‑1 (MQ=255)

                                                                                    cTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatga                                                       >  1:3762683/1‑140 (MQ=255)

                                                                                       cAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTTATGAGGc                                                    >  1:7185714/1‑140 (MQ=255)

                                                                                              cTCGGACCGTTCTTATATAACCCAACCAGTCTTTATTACCTGTATCATTATTCGATCGCATCCACCCCCTACCA‑AAAAACCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                             <  1:2819907/140‑1 (MQ=255)

                                                                                              cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                             >  1:5229637/1‑140 (MQ=255)

                                                                                              cTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTAACAGGCTTCGATCGTATGCAAGCCCTTCCC‑AAAAACCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGAAATTACAACATGATTATGCTTAagag                                             >  1:2393875/1‑140 (MQ=255)

                                                                                              cTCGCACAGGTCTTCTATAAATGAACCAGTGTTTATTACCTGTATCTTTATTCGATGTTATGCAACCCCTTCCA‑AAAACCGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                             <  2:6328974/140‑1 (MQ=255)

                                                                                                  caCAGTTCTTCTATAAATCACCCAGTCGTTTTTCCGTTTATAATTATTCGATCGTATGCACCCCGTTCCC‑AAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                         <  2:2167002/139‑1 (MQ=255)

                                                                                                  caCAGGTTTTATATAAATTAACCAGTCTTTTTTACCTTTATATTTATTCTATCGTATGAAAGCCCTTCCA‑AAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                         <  2:3486899/139‑1 (MQ=255)

                                                                                                   cgttgTCTTATATAAAGCAACCAGTGTTTTTTACTTGTATCATGTTTCGTTCGTAGGCAAGCCCTGCCC‑AAAACCCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATc                                        <  1:5824862/136‑1 (MQ=255)

                                                                                                      ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTTATGAGGCTTAAGAGCGATCATg                                     >  1:184260/1‑140 (MQ=255)

                                                                                                      ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAAAGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATg                                     >  1:605056/1‑140 (MQ=255)

                                                                                                           tctTTAAATGAACCAGTCTTTTTTAGCTTTATCATGATTCGATCGTATGAAAGCCCTACCA‑AAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                                <  2:7354461/140‑1 (MQ=255)

                                                                                                           tctATAACTCACCCTGTCTTTTTTACATTTATCATTTTTCGATCGTATGCAAGCCCTGCCA‑AAAAACCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                                <  2:5161021/140‑1 (MQ=255)

                                                                                                           tatataAATCACCCATTGTTTTTTACCTGTATATTTTTTCGATGGTAT‑CAACCCCCTCCCAAAAAACCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                                <  1:159238/138‑1 (MQ=255)

                                                                                                             tataAATGAACCAGTCGTGCTTACCTGTATCATGATTCGATCGTATGCCAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCACGTTCGCAAGACATAACAACTTGATGAGGCTTAATACCGATCATGTCTAAAt                              >  1:2624297/1‑140 (MQ=255)

                                                                                                                aaaTCAACCCGTCTTTTTTACCTGTATATTGATTCGTTCTTATGCACCCCCTTCCA‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGc                           <  2:1877699/140‑1 (MQ=255)

                                                                                                                  aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGGTTATTCAAGTTCGCAAGAAATAAAAACTTGATTAGGCTTAAGAGCGATCATGTATAAATTgacc                         >  2:3147212/1‑137 (MQ=255)

                                                                                                                   tGAACCAGTCGTTATTACCTGTATCATGATTGGATCGTATTCAAGCCCTTCCC‑AAAAACGATAAAACGAAGCTGCTTCTTCAAGTTCGCAAGACAGATCAACGTGCTGTGGCTTAATAGCGATCATGTCTAACTTGCCGa                        >  1:7433995/1‑140 (MQ=255)

                                                                                                                     aaCCAGTGGTTATTCCCTTTATATTTATTGGATCGTATGAACCCCCTTCCC‑AAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                      <  2:3031215/140‑1 (MQ=255)

                                                                                                                     aaCCAGTCGTTTTTACCTGTATCTTTATTCTATCGTAGCCACGCCCTTCCC‑AAAACCGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                      <  2:7185714/140‑1 (MQ=255)

                                                                                                                     aaCCAGGCGTTATTAGCTGTATGATGATTCGATCGTCTGCACGCCCTACCC‑ACTAACCCTAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                      <  2:5745161/140‑1 (MQ=255)

                                                                                                                                     cTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTa      >  1:108719/1‑140 (MQ=255)

                                                                                                                                        tATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt   >  2:5983143/1‑140 (MQ=255)

                                                                                                                                        tATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt   >  1:6738126/1‑140 (MQ=255)

                                                                                                                                        tATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt   >  2:2010731/1‑140 (MQ=255)

                                                                                                                                        tATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt   >  2:7671214/1‑140 (MQ=255)

                                                                                                                                        tATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGACTAAATTGCCGATACTGCTTATAGCGGTTAttt   >  2:3305561/1‑140 (MQ=255)

                                                                                                                                         aTCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTc  >  2:5644246/1‑140 (MQ=255)

                                                                                                                                         aTCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTc  >  1:6921848/1‑140 (MQ=255)

                                                                                                                                         aTCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTc  >  2:2546852/1‑140 (MQ=255)

CTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTC  >  NZ_CP034943/3851095‑3851371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`