BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,237	G→T	8.3%	I46I (ATC→ATA)	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,237	0	G	T	8.3%	1494.4 / 15.9	643	I46I (ATC→ATA)	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (409/141); new base T (14/74); total (424/219)
Fisher's exact test for biased strand distribution p-value = 6.44e-26
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 648 total aligned reads displayed.

CAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAA  >  NZ_CP034943/3851098‑3851376

   tGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                            <  1:4626811/140‑1 (MQ=255)

   tGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                            <  2:7091016/140‑1 (MQ=255)

     tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                          >  2:3123204/1‑140 (MQ=255)

     tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                          >  1:104262/1‑140 (MQ=255)

     tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                          >  2:1054141/1‑140 (MQ=255)

     tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                          <  2:3956735/140‑1 (MQ=255)

     tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                          >  1:2524235/1‑140 (MQ=255)

     tCCCAATCTGCTCTCTGTAAGATTGATTGGAAATGGAATTCTCTTTTCGCTACTATTATCACCTTGTAAGCAGAACAATCAAAGTACTCGGAAAGGTCTTCTATAAATGAACCAGAGCTTAGTCCCTGTATCATggatcg                                                                                                                                          >  2:4501352/1‑135 (MQ=255)

     acccAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                          >  2:6535058/2‑140 (MQ=255)

       ccAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGAt                                                                                                                                        <  1:5401990/140‑1 (MQ=255)

        cAATCTGCTCTCTGTACTATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                       <  1:3846583/140‑1 (MQ=255)

        cAATCGGCTCTCTGTAATAGGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                       <  1:7424436/140‑1 (MQ=255)

           tCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTa                                                                                                                                    >  2:3470373/1‑140 (MQ=255)

             tGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATg                                                                                                                                  >  2:4133100/1‑140 (MQ=255)

               ctctctGTAATATGGATTGTAAATGGAATTCTCTTTTCGCTAGTTTTAACACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAAACAGTCGTTATTACCAGTATCATTATTCGATCGTATGCa                                                                                                                                >  1:7052711/1‑140 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGTACCAGTCGGTATTACCTGTATCATGATTAGATCGTATTCa                                                                                                                                >  1:1183459/1‑140 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                                >  2:6652047/1‑140 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                                >  1:2141420/1‑140 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                                >  1:5903697/1‑140 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                                >  1:3129467/1‑140 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                                >  2:6306762/1‑140 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTAAAAGTACTAGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATgaa                                                                                                                                >  1:7473820/1‑138 (MQ=255)

                 ctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                       >  2:3718234/1‑131 (MQ=255)

                  tctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGc                                                                                                                             >  2:1731100/1‑140 (MQ=255)

                    tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                           >  1:3576315/1‑140 (MQ=255)

                    tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                           <  1:374423/140‑1 (MQ=255)

                    tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                           >  1:4551699/1‑140 (MQ=255)

                    tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                           >  1:7250889/1‑140 (MQ=255)

                    tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAATGATTCGATCGTATGCAAGccc                                                                                                                           >  1:5777953/1‑140 (MQ=255)

                     gTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCt                                                                                                                          >  1:5055525/1‑140 (MQ=255)

                         tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTccc                                                                                                                      >  1:1374502/1‑140 (MQ=255)

                         tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACATGTATAATGATTCGATAGTATGCAATCCCttaca                                                                                                                      >  1:4169020/1‑137 (MQ=255)

                              aTTGGAAATGGAATTGTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGTACCAGGCGTTATTACCTGTATCATGATTCGCTCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                                >  2:495167/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAGCCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCAAGCCCTTCCC‑aaaaa                                                                                                                >  1:2660605/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGACCCAGTCGTTATTACCTGTCTCATGATTCGAGCGTATGCGCGCCCTTCCC‑aaata                                                                                                                >  1:6530820/1‑138 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGCTCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                                >  1:4718159/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCACGCCCTTCCC‑aaaaa                                                                                                                >  1:6356191/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                                >  2:7528143/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                                >  2:3345982/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                                >  1:2282336/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                                >  2:1478349/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                                >  1:1981673/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                                >  2:5427724/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                                >  1:6662041/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAATGATTCGATCGTATGCGAGCCCTTCCC‑aaaaa                                                                                                                >  1:32235/1‑140 (MQ=255)

                                tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAATTACTCGGACAGCTCTTCTATAAATGAAACAGTCGTTATTACATGTATCATTATTAGATAGTATGAAAGACCTTCAC‑aaaaaat                                                                                                              >  2:6411803/1‑138 (MQ=255)

                                       ggAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAA‑CGATAAAAc                                                                                                      >  2:407010/1‑140 (MQ=255)

                                        gAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAA‑CGATAAAACg                                                                                                     >  2:5276907/1‑140 (MQ=255)

                                               attttCGCTATTTTTATCACCTTGTAAGCATAACCTAAAAATTACTCGCACTTTTGTTTTATAAATCACCCCGCCTTTATTACTTTTTTCTTTTTCCCATGGGTTCCACCCCCTTCCC‑AAAAA‑CTATAACACGACTCTGc                                                                                              <  2:6796410/139‑1 (MQ=255)

                                                   tCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAA‑CGATAAAACGACTCTGcttct                                                                                          >  2:89929/1‑140 (MQ=255)

                                                            ttATCACTTTTTAACAATAACCTTAAAATTACTCGCACTGTTTTATTATAAATGAACCTGTGTTTTTTACCTTTACAATTATACGTTCTTATGAAAGCCTTTCCA‑AAAAA‑CGTAAAAACGACTCTGCTTCTTCAAGTTCg                                                                                 <  2:3614751/140‑1 (MQ=255)

                                                            ttATCACCTTGTAAGAATAACCTTCAATTTACTCGGACTGGTATTCTATAAATCAACCAGTCTTTTTTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCg                                                                                 <  2:3577068/140‑1 (MQ=255)

                                                            ttATCAAATTGTACGCATACCCTCAAATGTACTCCGACAGGTATTCTATAAATCAACCAGTCTTTATTACCTGTATCATTATTGGAGCTTAAGCACGCCCTTCCA‑AAAAA‑CTATAAAACGACTCTGCTTCTTCAAGTTCg                                                                                 <  2:7136404/140‑1 (MQ=255)

                                                                    ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAt                                                                         >  1:6049349/1‑140 (MQ=255)

                                                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                >  2:1981402/1‑140 (MQ=255)

                                                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAA‑CGATAAAACGACTCTGCTTCATCAAGTTCGCAAGACATAACAACTtg                                                                >  1:7121713/1‑140 (MQ=255)

                                                                                ccTTCAATTTACTCGTACTGGTCTTCAATAAATCAACCAGTGTTTATTACCTTTATAATTATTCGATCGTATCCAAGCCCTTCCC‑AAAAA‑CCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:6934491/140‑1 (MQ=255)

                                                                                ccTTCAAAGTCCGCGGAAAGTTCTTCTATAAATCAACCAGTCGTTTTTACCTGTATCATGTTTCGATCGTCTCCAAGCCCTTCCC‑AAAAA‑CGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:1636198/140‑1 (MQ=255)

                                                                                ccTTCAAAGTACTCGCACAGGTATTATATAAATGAACCAGTCTTTTTTACCTGTATCATTATTCGATCGTATGCAAGCCCTTCCA‑AAAAA‑CTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:7250889/140‑1 (MQ=255)

                                                                                ccTTAAAAGTACTCGCACAGGGTTTCTATAAATCAACACGTCTATATTACCTTTATAATGATTCGATCGTATGCAAGCCCTGCCC‑AAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:4399151/140‑1 (MQ=255)

                                                                                ccTACAACGTACTCGGACCGGTATTCTATAAATGACCCCGTGTTTATTACCTGTAACATGATTCCATGGAAGGCACCCCCTCCCA‑AAAAC‑CGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:2952512/140‑1 (MQ=255)

                                                                                ccTACAAATTACTCGGACAGGTCTTCTATAAATGAACCAGTCTTTATTACCTGTATCATTATTCGATCGTATGCACGCCCTTCCA‑AAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:5730189/140‑1 (MQ=255)

                                                                                ccTACAAAGTACTCGGACTGGTCTTATATAAATGAACCATTGTTTATTACCTGTATCATTTTTCGATCGTATGAAAGCCCTACCC‑AAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:1834266/140‑1 (MQ=255)

                                                                                ccTACAAAGTACCCGCACCGGTCTTCAATAAATCAACCAGTCTTTTTTACCTGTAACTTCATGCGATCGTCTGAAACCCCGACCA‑AAAAC‑CGAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:2255893/140‑1 (MQ=255)

                                                                                           cTCGGACCGTTCTTATATAACCCAACCAGTCTTTATTACCTGTATCATTATTCGATCGCATCCACCCCCTACCA‑AAAAA‑CCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                  <  1:2819907/140‑1 (MQ=255)

                                                                                           cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                  >  1:864791/1‑140 (MQ=255)

                                                                                            tCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGATGCTTAAGAGc                                                 >  1:5364854/1‑140 (MQ=255)

                                                                                            tCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAA‑CGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGAAATAACAACTTGATGAGGCTTAAGAGc                                                 >  2:5874581/1‑140 (MQ=255)

                                                                                            tCGGACAGGTCTTCAATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCAAGCCCTTCGC‑AAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTAGCAATACATAACAACTTGATGAGGCTAAAGAGc                                                 >  1:3405116/1‑140 (MQ=255)

                                                                                               gACTGGTCTAATAAAAATGAACCAGTCTTTATTACTTTTATAATTATTCTATCTTATGCAAGCCCTGCCC‑AAAAC‑CTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                              <  2:2470175/140‑1 (MQ=255)

                                                                                               gACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                              >  1:5194822/1‑140 (MQ=255)

                                                                                                aCAGTTATTCTATAAATCAACCTTTCTTTATTACCTGTATCTTTTTTCGATCTTATGCACGCCCTCCCC‑AAAAA‑CCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATc                                             <  2:4588301/140‑1 (MQ=255)

                                                                                                   ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTTATGAGGCTTAAGAGCGATCATg                                          >  1:184260/1‑140 (MQ=255)

                                                                                                    gTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAATAGCGATCATGt                                         >  2:1241898/1‑140 (MQ=255)

                                                                                                        tatttaAACGACCCCGTCGTTTTTACCTGTATCTTTTTTCGAGCGTATGCACGCCCTACCA‑AAAAA‑CTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                                     <  2:2769720/136‑1 (MQ=255)

                                                                                                        tatataAATGAACCAGTCTTTTTTACTTTTTTAATGATTCGATCTTATGAAAGCCCTTCCA‑AAAAA‑CTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                                     <  2:510/138‑1 (MQ=255)

                                                                                                        tatataAATCACCCATTGTTTTTTACCTGTATATTTTTTCGATGGTAT‑CAACCCCCTCCCAAAAAA‑CCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                                     <  1:159238/138‑1 (MQ=255)

                                                                                                         ctATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAA‑CGATAAAACGACTCTGCTTCTTCAACTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTtaa                                    >  1:5563628/1‑140 (MQ=255)

                                                                                                          tataAATGAACCAGTCTTTTTTACCTTTATCATTATTCTATCTTATGCAACCCCTGCCC‑AAAAA‑CCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAt                                   <  2:4261478/140‑1 (MQ=255)

                                                                                                            taACTGAACCTGTCGTTAGTACCTGTATCATGTTGCGATGTTATTCAAGCCCTGCCA‑AAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTg                                 <  2:2373227/140‑1 (MQ=255)

                                                                                                             aactcaaCCAGTCTTTATTACCTGTATAATGATTCTATCGTATGCAACCCCTCCCA‑AAAAC‑CCTTAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGc                                <  2:5997149/135‑1 (MQ=255)

                                                                                                             aaaTCAACCATTGTTTATAACCTTTATAATCATTCGATCTTAGGCAAGCCCTGCCA‑AACAA‑CCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGc                                <  2:2630206/140‑1 (MQ=255)

                                                                                                              aaTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACCTAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGcc                               >  1:4306764/1‑140 (MQ=255)

                                                                                                              aaTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAACACATAACAACTTGATGATGCTTAATAGCGTTCATGTCTAAATTGcc                               >  2:3841424/1‑140 (MQ=255)

                                                                                                                  aaCCAGTGTTTATTACCTTTATCATTATGCGATCGTATGCAAGCCCTTCCC‑AAAAC‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                           <  1:7332752/140‑1 (MQ=255)

                                                                                                                  aaCCAGTGGTTATTACCTTTATCATGATTCGATCGTATGCACGCCCTTCCA‑AAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                           <  2:5352934/140‑1 (MQ=255)

                                                                                                                  aaCCAGTCTTTATTACCTTTATAATTATTCGATCTTATGAAAGCCCTACCA‑AAAAA‑CTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                           <  2:6059169/140‑1 (MQ=255)

                                                                                                                  aaCAAGTCTTTATAACCTTTATCATTATTCCATCGTA‑GCAAACCCAGACC‑AAAAACCTAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                           <  1:304513/140‑1 (MQ=255)

                                                                                                                   aCCAGTCGGTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAA‑CGATTACAAGACTCTGCTTAGTGATGTTCGGACGCGATAACAACGTGAGGTGGGTTGAGGGCGATCATGTCGCAATTGCCGATAc                          >  2:2132127/1‑140 (MQ=255)

                                                                                                                    ccAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AACAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGGCTAAATTGACGATACt                         >  2:7636666/1‑140 (MQ=255)

                                                                                                                    ccAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                         >  2:733688/1‑140 (MQ=255)

                                                                                                                          gTTATTCCCTGTATCATGATTCGATCTTATCCAACCCATACCC‑AAAAA‑CTATACAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTtat                   <  2:7433995/140‑1 (MQ=255)

                                                                                                                                  cTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTa           >  1:108719/1‑140 (MQ=255)

                                                                                                                                   tttttCATTATTCGATCTTTTGCAAGCCCTACCA‑AAAAA‑CCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAt          <  2:469459/136‑1 (MQ=255)

                                                                                                                                   tttATCTTGATTCGATCGTAGGCAACCCCTTCCA‑AAAAA‑CCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAt          <  1:2412013/138‑1 (MQ=255)

                                                                                                                                     tATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt        >  1:6738126/1‑140 (MQ=255)

                                                                                                                                     tATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt        >  1:4027784/1‑140 (MQ=255)

                                                                                                                                      aTCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTc       >  2:3206663/1‑140 (MQ=255)

                                                                                                                                         aTGATTCGATCGTATGCAAGCCCTTCCC‑AAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCAt    >  1:6874127/1‑140 (MQ=255)

                                                                                                                                          tGATTCGATGGTAGGCAAGCCCTCCCA‑AAAAA‑CGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATa   <  1:6117233/140‑1 (MQ=255)

CAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAA  >  NZ_CP034943/3851098‑3851376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`