BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,254	G→C	8.9%	L41V (CTT→GTT)	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,254	0	G	C	8.9%	1172.3 / 24.4	561	L41V (CTT→GTT)	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (354/136); new base C (4/54); total (369/192)
Fisher's exact test for biased strand distribution p-value = 6.02e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 587 total aligned reads displayed.

CTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAG  >  NZ_CP034943/3851115‑3851393

   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTTTATAATGATTCGATCGTATGCAATccc                                                                                                                                           >  1:6463104/1‑140 (MQ=255)

   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                                           <  1:4608915/140‑1 (MQ=255)

   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                                           >  2:3974024/1‑140 (MQ=255)

   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                                           <  1:374423/140‑1 (MQ=255)

   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                                           >  1:3576315/1‑140 (MQ=255)

   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                                           >  1:7118451/1‑140 (MQ=255)

   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                                           >  1:5718945/1‑140 (MQ=255)

   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTAGATCGTATGCAAGccc                                                                                                                                           >  1:2255893/1‑140 (MQ=255)

        tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATATCCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGGTCTTACCTGTATCAGTAGTCGTTCGGATGCAAGCCCGTccc                                                                                                                                      >  1:7511670/1‑140 (MQ=255)

        tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTccc                                                                                                                                      >  2:2461197/1‑140 (MQ=255)

        tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTccc                                                                                                                                      >  1:2325778/1‑140 (MQ=255)

        tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTacc                                                                                                                                      >  1:469459/1‑140 (MQ=255)

        tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCGTccc                                                                                                                                      >  1:6197678/1‑140 (MQ=255)

        tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGCAACCCCTTccc                                                                                                                                      >  2:111195/1‑140 (MQ=255)

        tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGCAACACCTTccc                                                                                                                                      >  2:3879395/1‑140 (MQ=255)

        tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTAACTTTATCATGATTCGATCGTATGCAAGCCCTTccc                                                                                                                                      >  1:3180999/1‑140 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCGGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                 >  2:1497651/1‑140 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAATCCCTTCCCaaaaa                                                                                                                                 >  1:1493517/1‑140 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                 >  1:6022480/1‑140 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                 >  2:159239/1‑140 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                 >  1:5512433/1‑140 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                 >  1:911318/1‑140 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                 >  2:6203583/1‑140 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                 >  2:5958206/1‑140 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGAAAGCCCTTCCCaaaaa                                                                                                                                 >  2:4079498/1‑140 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTAACTGTATCATGATTAGATAGTATGCAAGCCCTTCCCaaaaa                                                                                                                                 >  2:4292936/1‑140 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTAACAGTATCATTATTCGATCGTATGAAAACCCTTCCCcaaaa                                                                                                                                 >  1:3908146/1‑140 (MQ=255)

             aTTGGAAATGGAATTATCTTTTCTCTAGTTTTATCACCTTGTAAGCATAACATTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTTCCTGTATCATGATTCTATCGTATGCAAGCACGTCCCaaaaa                                                                                                                                 >  1:7025927/1‑140 (MQ=255)

               tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAATTACTCGGACAGCTCTTCTATAAATG‑AAACAGTCGTTATTACATGTATCATTATTAGATAGTATGAAAGACCTTCACaaaaaat                                                                                                                               >  2:6411803/1‑138 (MQ=255)

                 gAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCGGTATCAAGATTCGATCGTATTCAAGCCCTTCCCAAAAA‑CGAt                                                                                                                            >  1:4792851/1‑140 (MQ=255)

                      ggAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAAc                                                                                                                       >  2:5423325/1‑140 (MQ=255)

                            ctctTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAAAGAatct                                                                                                                 >  1:6371137/1‑140 (MQ=255)

                              attttCGCTATTTTTATCACCTTGTAAGCATAACCTAAAAATTACTCGCACTTTTGTTTTATAAATC‑ACCCCGCCTTTATTACTTTTTTCTTTTTCCCATGGGTTCCACCCCCTTCCCAAAAA‑CTATAACACGACTCTGc                                                                                                               <  2:6796410/139‑1 (MQ=255)

                                    gatttttttACCACCTTGTAAGCAAAACATTCAACTTACTCGCCCAGGTCTTATATAAATC‑AACCAGTCGTTATTACCTGTATCATTATTCGATCTTATGCAACCCCTCCCCAAAAC‑CGATAAAACGACTCTGCttcttc                                                                                                         <  2:7078566/135‑1 (MQ=255)

                                       aGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAg                                                                                                      >  2:7577727/1‑140 (MQ=255)

                                           ttATCAAATTGTACGCATACCCTCAAATGTACTCCGACAGGTATTCTATAAATC‑AACCAGTCTTTATTACCTGTATCATTATTGGAGCTTAAGCACGCCCTTCCAAAAAA‑CTATAAAACGACTCTGCTTCTTCAAGTTCg                                                                                                  <  2:7136404/140‑1 (MQ=255)

                                                   ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCAAGCCCTTCCCAAAAA‑AGATAAAAAGACTATTCTTATTCAAGTTCTCAAGACAt                                                                                          >  2:4476049/1‑140 (MQ=255)

                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                 >  2:3364661/1‑140 (MQ=255)

                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                 >  1:5867655/1‑140 (MQ=255)

                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                 >  2:1981402/1‑140 (MQ=255)

                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                 >  2:3651453/1‑140 (MQ=255)

                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACCACTCTGCTTCTTCAAGTTCGCAAGACCTAACAACTtg                                                                                 >  1:1096466/1‑140 (MQ=255)

                                                             aaCCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACttta                                                                                >  1:738343/1‑138 (MQ=255)

                                                               cgtaaaaaTTACTCGCAATGGTTTTATATAAATC‑AACCAGTGTTTATTACCTGTATAATTATTCCCTCTTATCCAAGCCATACCAAAAAA‑CCAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:853192/135‑1 (MQ=255)

                                                               cctaaaaaTTACTCGGACTGTTCTTATATAAATC‑AACCAGTCTTTATTACCTTTAACATGATTCGCTCGTATGCAAGCCCTGCCAAAAAA‑CTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:4964486/135‑1 (MQ=255)

                                                               cctaaaaaTTACCCGCACTGGTATTATAAAAATC‑AACCCGTGTTTTTTCCCTTTATCTTTATTCCATCGTTTGAAACCCCTCCCAAAAAA‑CCAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  1:682628/135‑1 (MQ=255)

                                                               ccTTCAATTTACTCGTACTGGTCTTCAATAAATC‑AACCAGTGTTTATTACCTTTATAATTATTCGATCGTATCCAAGCCCTTCCCAAAAA‑CCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:6934491/140‑1 (MQ=255)

                                                               ccTTCAAATTACTCGGACAGGTTTTATCAAAATC‑ACCCTGTGTTTATTCCTTGTTGCATGTTGCCACCTTAGCCACGCCGTACCCAACAC‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:3142448/140‑1 (MQ=255)

                                                               ccTTCAAAGTAGGCGTACGGGTCTTATAAAACTG‑ACCCAGTGTTTATTACCTCAATCATTATCCGTAGGTATGCACCCCCTACCCAAAAA‑CCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:4749151/140‑1 (MQ=255)

                                                               ccTTCAAAGTACTCGTCACGGTTTTCTAAAAATC‑AACCAGTCTTTTTTACGTTTACATTTTTTCGTTCTAAGCCAACCCCTACCAAAAAA‑ATATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  1:7211836/140‑1 (MQ=255)

                                                               ccTACAAAGTACCCGCACCGGTCTTCAATAAATC‑AACCAGTCTTTTTTACCTGTAACTTCATGCGATCGTCTGAAACCCCGACCAAAAAC‑CGAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:2255893/140‑1 (MQ=255)

                                                               cattaaaaTTACTGGGACAGTTCTACTATAAATT‑AACCAGCCGTTATTACGTTTATCATTTTTCCACCGTATGAAACCCCTACCAAAAAA‑CTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:3757162/135‑1 (MQ=255)

                                                               cataaaaaGTCCTCGGACCGGTAGTATATAATTT‑AACCAGTCGTTATTACGTGTATCATGTTGCGATCGTATGCAAGCCCGACCCAAAAC‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACGggatg                                                                              <  2:1776063/135‑1 (MQ=255)

                                                                    aaaGTACTCGGACAGGGCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGTGGCt                                                                         >  2:1688214/1‑140 (MQ=255)

                                                                          cgcgcaCATGTATTCTAAAACTC‑AACCCTTCTTTTTTACCTTTATCATTATTCCACGTTATCAAAGCCCTACCAAAAAA‑CGAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                                   <  2:3026028/135‑1 (MQ=255)

                                                                          cTCGGACTGGTATTCTATAAATC‑AACCACTCGTTAGTCCCTGTATCCTGTTTCTATATTATGAAAGCCCTCCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGATTAagag                                                                   <  2:7240056/140‑1 (MQ=255)

                                                                          cTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAACTTAGCAAGACAAAACAAAATGATTAGGCTTAagag                                                                   >  1:6654449/1‑140 (MQ=255)

                                                                           tCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGATGCTTAAGAGc                                                                  >  1:5364854/1‑140 (MQ=255)

                                                                           tCGGACAGGTCTTCTATAAATG‑AACCAGTCGGTATTACCTGGATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCATACATTTCCCAAGACATAACAACTTGCAGAGGGTTAAGAGc                                                                  >  1:1299046/1‑140 (MQ=255)

                                                                              gACAGGTCTTCTATAAATG‑AACCAGTCTTTATTACCTGTATCATTTTTCGATCGTATCCAAGCCGTTCCCAAAAA‑CGTAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                                               <  2:277621/140‑1 (MQ=255)

                                                                              gACAGGTCTTCTA‑ACATGTCACCCGTCTTTATCACCTGTATATTGATTCGATCGTAGCCACCCCCGTCACAAAAC‑CTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                                               <  1:4083398/140‑1 (MQ=255)

                                                                              caCAGGTTTTATATAAATT‑AACCAGTCTTTTTTACCTTTATATTTATTCTATCGTATGAAAGCCCTTCCAAAAAA‑CTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                                               <  2:3486899/139‑1 (MQ=255)

                                                                                  ggTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATg                                                           >  2:6520820/1‑140 (MQ=255)

                                                                                  ggTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATg                                                           >  2:3583420/1‑140 (MQ=255)

                                                                                   gacttctATACAAC‑ACCCAGCCTTTATTACAGGTAACTTGATGCGAGCGTAGCCACCCCCCCCCAAAAAA‑CCAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGt                                                          <  1:6073532/138‑1 (MQ=255)

                                                                                       tatataAATG‑AACCAGTCTTTTTTACTTTTTTAATGATTCGATCTTATGAAAGCCCTTCCAAAAAA‑CTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                                                      <  2:510/138‑1 (MQ=255)

                                                                                         tataAATG‑AACCAGTCGTGCTTACCTGTATCATGATTCGATCGTATGCCAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCACGTTCGCAAGACATAACAACTTGATGAGGCTTAATACCGATCATGTCTAAAt                                                    >  1:2624297/1‑140 (MQ=255)

                                                                                           taACTG‑AACCTGTCGTTAGTACCTGTATCATGTTGCGATGTTATTCAAGCCCTGCCAAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTg                                                  <  2:2373227/140‑1 (MQ=255)

                                                                                              aTG‑AACCAGTCGTTATTACCTGTATAATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGTCTTAAGAGCGATCATGTCTAAATTGCCg                                               >  2:4572810/1‑140 (MQ=255)

                                                                                               tG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGa                                              >  1:6721179/1‑140 (MQ=255)

                                                                                                  caCCTGTCGTTATTACCTGTATCATGATTCGAAGGTATGCAACCCCTGCCCAAAAC‑CCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                            <  2:5170315/139‑1 (MQ=255)

                                                                                                  acccAGTGGTTCTTCCCTTTATCTTTTTTCTCTCGTATGCAAGCCCTCCCCAAAAC‑CTATACCACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                            <  1:2848316/138‑1 (MQ=255)

                                                                                                  acccAGTCTTAATAACCTGTATCATGATTCTTTCTTATGAAAGCCCTTCCAAACAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGAGATCATGTCTAAATTGCCGATa                                            <  2:7520146/138‑1 (MQ=255)

                                                                                                  aaacAGTCTTTATTACCTGTAACATTTTTCGATCGTTTGCAAGCCCTTCCCAAAAA‑CCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                            <  2:1325555/137‑1 (MQ=255)

                                                                                                  aaCCAGTTTTTTTTACCTGTATCTTGATTCGATTTTATGAAACCCCTACCAAAAAA‑CTAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                            <  1:4476049/140‑1 (MQ=255)

                                                                                                  aaCCAGTCTTTTTTACCTTTATCATTATTCGATCGTTGGACACCCCTACCAAAAAC‑CGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                            <  1:3476209/140‑1 (MQ=255)

                                                                                                  aaCCAGGCGTTATTAGCTGTATGATGATTCGATCGTCTGCACGCCCTACCCACTAA‑CCCTAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                            <  2:5745161/140‑1 (MQ=255)

                                                                                                   aCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTTCCGATAc                                           >  2:665775/1‑140 (MQ=255)

                                                                                                   aCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCGTCAAGTTCGCAAGACATAACAACATGATGAGGCTTAATAGCGATCATGTATAAATTGCCGATAc                                           >  1:7321769/1‑140 (MQ=255)

                                                                                                   aCCAGTCGGTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATTACAAGACTCTGCTTAGTGATGTTCGGACGCGATAACAACGTGAGGTGGGTTGAGGGCGATCATGTCGCAATTGCCGATAc                                           >  2:2132127/1‑140 (MQ=255)

                                                                                                    ccAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTAGCAAGACATAACAACTTGATGAGGCTTAAGATCGATCATGTATAAATTGCCGATACt                                          >  2:7400433/1‑140 (MQ=255)

                                                                                                        tCGTTATTACCTGTATCATGATACGATCGTATGCAAGCCCTTCCCAAAAA‑CCATAAAACGACTCAGCTTCTTCAAGTTCGCAACACAAAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCtt                                      >  1:509107/1‑140 (MQ=255)

                                                                                                         cgtatttaCCTGTATCTTGATTCGATCGTATGCAAGCCCTTCCAAAAAC‑CGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTta                                     <  2:5556799/135‑1 (MQ=255)

                                                                                                                  cTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAATACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTa                            >  1:2064778/1‑140 (MQ=255)

                                                                                                                  cTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTa                            >  1:1693389/1‑140 (MQ=255)

                                                                                                                  cTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTa                            >  1:1849995/1‑140 (MQ=255)

                                                                                                                  cTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAAATTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTa                            >  1:2468851/1‑140 (MQ=255)

                                                                                                                     tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGATGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt                         >  2:3303380/1‑140 (MQ=255)

                                                                                                                     tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCTGtctctt                         >  1:3099675/1‑135 (MQ=255)

                                                                                                                      aTCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTc                        >  2:2546852/1‑140 (MQ=255)

                                                                                                                      aTCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTc                        >  2:5315533/1‑140 (MQ=255)

                                                                                                                          tGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATa                    >  2:6009297/1‑140 (MQ=255)

                                                                                                                               ctaTCGTAGGCAACCCCTTCCCAAAAA‑CCAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATc               <  2:4231527/138‑1 (MQ=255)

                                                                                                                               cGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATc               >  2:1846086/1‑140 (MQ=255)

                                                                                                                                 aTCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCtt             >  2:4655528/1‑140 (MQ=255)

                                                                                                                                  ttcgtctcAACCCCGTCACAAAAACCTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCtt             <  1:4221701/133‑1 (MQ=255)

                                                                                                                                    ttATGAAAGCCGTGCCAAAAAA‑CTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTccc          <  2:6049349/139‑1 (MQ=255)

                                                                                                                                       tccacgCCCTCCCCAAAAA‑CGAAAAAACGAATCTGCTTCTTCAATTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATGATGTCTACATTGCCGATACTGCTTATAGCGGTTTTTTCCATAAAATCTTCCCCtt       <  2:2695985/135‑1 (MQ=255)

                                                                                                                                       tGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt       <  2:7330175/140‑1 (MQ=255)

                                                                                                                                            gCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg  >  1:105153/1‑140 (MQ=255)

CTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATG‑AACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAG  >  NZ_CP034943/3851115‑3851393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`