BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,529	G→C	6.4%	A124A (GCG→GCC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,529	0	G	C	6.4%	401.4 / 3.5	160	A124A (GCG→GCC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base G (79/64); new base C (3/9); total (85/75)
Fisher's exact test for biased strand distribution p-value = 6.82e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 163 total aligned reads displayed.

GGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAG  >  NZ_CP034943/607391‑607665

ggAAGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACGGGAGCGACCGGGGCGCCCGGGGCTCCCGGTCCCGCCGGAC‑CCGCGGGAGCGCGCGGAGCgg                                                                                                                                          >  2:1810846/1‑139 (MQ=2)

ggAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGACAGGCGCGACCGGCGCTCCCGGGGACGCGGGCC‑CAGCGGGAGCGACGGGAGCgg                                                                                                                                          >  2:1687891/1‑139 (MQ=14)

 gAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGAc                                                                                                                                         <  1:182844/140‑1 (MQ=255)

   agccggaccagccggaccAACCGGAGCGACAGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAg                                                                                                                                       <  1:3679246/140‑1 (MQ=255)

    gccggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAgg                                                                                                                                      >  2:2286899/1‑140 (MQ=255)

    gccggaccagccggaccaACCGGAGCCACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAgg                                                                                                                                      <  1:4310558/140‑1 (MQ=255)

                   ccaCCCGGAGCGACCGGAGCCACCGGAGCTCCGGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCGGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGc                                                                                                                      <  2:3848465/140‑1 (MQ=14)

                   ccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGc                                                                                                                      >  1:886348/1‑140 (MQ=255)

                     aaCCGGAGCGACAGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCg                                                                                                                    <  1:4782528/140‑1 (MQ=255)

                      aCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCgg                                                                                                                   >  2:3782162/1‑140 (MQ=255)

                      aCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCgg                                                                                                                   >  2:3448296/1‑140 (MQ=255)

                      aCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCGGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCgg                                                                                                                   >  1:2476640/1‑140 (MQ=255)

                       ccGGAGCGTCAGGAGCGACAGGTGCTACCGGGGAAGCCGGCCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGa                                                                                                                  <  1:3823325/140‑1 (MQ=255)

                       ccGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCTACAGGAGCGACAGGAGCGACAGGAGCTCCCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGa                                                                                                                  <  1:4809212/140‑1 (MQ=255)

                       ccGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGa                                                                                                                  <  1:3391246/140‑1 (MQ=255)

                        cgccgcgactgcagccaccggaGCTACCGGGGAAGCCGGAGCAGCCGGACCGACCGGAGCGCCCGGAGAGACAGGAGCTACCGGGGAATCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGAc                                                                                                                 <  2:3261794/136‑1 (MQ=14)

                        cGGAGCGACAGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGAc                                                                                                                 <  2:4974535/140‑1 (MQ=255)

                        cGGACCGCCAGGAGCGACCGGAGCTTCTGGGGAACCCTGACCAGGGGTCGCGCATGTGGAGACAGGACCGACAGGAGCTCCCGGGGAAGATGGAC‑CAGCTGGAGGGACGGGAGCCACAGGAGCTACCG‑GGGAAGCCGGAc                                                                                                                 <  1:3443403/140‑1 (MQ=9)

                           agcgacaggagcgacaggAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCAACCGGGGCAGCTGGAC‑CCGCTGGAGCGACTGGAGCTACAGGAGCTACCG‑GGGAAGCCGGAACAg                                                                                                              >  2:2916332/1‑140 (MQ=18)

                                 tggagcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGTGCGACAGGAGCTACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCgggg                                                                                                        <  2:3344984/139‑1 (MQ=255)

                                 aggagcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCgggg                                                                                                        >  1:4691655/1‑140 (MQ=255)

                                 aggagcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGATACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACTGGAGCTACCG‑GGGAAGCCGGACCAGCCgggg                                                                                                        >  1:143021/1‑140 (MQ=255)

                                     gcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGCGCCACCGGAGCAACCGGGGCAGCTGGAC‑CAGCGGGAGCCACCGGAGCGACAGGAGCTACCG‑GGGAACCCGGAGCAGCCGGGGCCAc                                                                                                    >  2:4809708/1‑140 (MQ=17)

                                     gcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCAc                                                                                                    >  2:430940/1‑140 (MQ=255)

                                     gcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCAACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGCAGCCGGACCAGCCGGGGCCAc                                                                                                    >  2:1990038/1‑140 (MQ=255)

                                        ccaggagcTACCGGGGAAGCCGGACCAGCCGCAGCGACAGGAGCCACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                 <  1:2060874/139‑1 (MQ=255)

                                        actggcgcTACTGGTGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                 <  1:5078098/134‑1 (MQ=255)

                                        acaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACCGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                 <  1:4785591/140‑1 (MQ=255)

                                        acaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                 <  1:3132458/140‑1 (MQ=255)

                                        acaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                 >  2:1119664/1‑140 (MQ=255)

                                        acaggagcTACCGGGGAAGCCGCACCAGCCGGAGCTACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCGGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                 <  1:4600406/140‑1 (MQ=255)

                                        acagcagcACCCGGCGAAGCCCGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCTGGGAAGCTTGAC‑CAGCTGGAGCTACGGTGGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                 <  1:4147513/140‑1 (MQ=18)

                                              gcTCCCGGGGAAGCCGCACCAGCCGGAGCGACAGGCGCGACAGGAGCGACAGGAGATACCTGGGAAGCTGGAC‑CAGTTGTAGCTACTGTAGAGACATGAGCTACCG‑GGTTAGCAGGACCAGCCGGGGCCACGGGAGCcac                                                                                           <  1:1652818/140‑1 (MQ=255)

                                              gcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                                                           <  1:3782162/140‑1 (MQ=255)

                                               cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                                          <  1:430940/140‑1 (MQ=255)

                                               cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                                          <  1:2286899/140‑1 (MQ=255)

                                                 cccGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                                        <  1:2107229/139‑1 (MQ=255)

                                                 aCCGGGGACGCCGGACCCGGCGGAGGCACCGGTGCCCCAGGCGCGTCCGGAGCAACCGGGCCCCCTGGCC‑CACCGGGAGCCACGGGAGCCACAGGACCAACCG‑GGGAAGCCGGACCCGCGGGGGCCACGGGAGCCACAgg                                                                                        <  2:2157211/140‑1 (MQ=255)

                                                   cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgggg                                                                                      >  2:2852027/1‑140 (MQ=255)

                                                      ggcaGCCGGACCAGCCGGAGCACCAGGAGCGACAGGAGCCACAGGAGCTACCGGGGAAGCCGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                                   <  1:4626058/137‑1 (MQ=18)

                                                      ggAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTAACGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                                   <  1:1619222/140‑1 (MQ=255)

                                                       gAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGATACCGGGCAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGCAGCTACCG‑CGGAAGCCGGACCACCCCGGGCCACGGGAGCCACCGCGGCGAc                                                                                  >  2:4177203/1‑140 (MQ=18)

                                                          gCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCCACAGGAGCTACCGTGGCAGATGGACACAAAT‑CCGCGACCGGCGGAACACGACCTAACG‑GGGAAGCCGGACCAACCGGTGCAACAGGAGCCACGGGGGCTACggg                                                                               >  1:4974829/1‑140 (MQ=12)

                                                             ggACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACCGGAGCTACCG‑GGGAAGCCGGACCCCCCGGGGCCACCGGCGCCACAGGGGCTCCTGGGGa                                                                            >  2:4013500/1‑140 (MQ=21)

                                                               aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGCCGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCCGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGacg                                                                          >  2:4126772/1‑138 (MQ=255)

                                                               aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCCACAgggccag                                                                          >  2:4320185/1‑136 (MQ=17)

                                                                    ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACCGGAGCTACCG‑GCGAAGCCGGACCAGCCGGGGCCACGGGAGCGACAGGGGCTCAGGGGGAAGCCGGa                                                                     >  2:1587934/1‑140 (MQ=21)

                                                                    ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGa                                                                     >  1:4070879/1‑140 (MQ=255)

                                                                        agcgacaggagcgacaggagcgacaggAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAg                                                                                                                                    >  1:4777917/1‑74 (MQ=255)

                                                                        agcgacaggagcgacaggagcgacaggAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAg                                                                                                                                    <  2:4777917/74‑1 (MQ=255)

                                                                          ctacaggagcgacaggagcgacaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGcc                                                               <  1:2040263/138‑1 (MQ=255)

                                                                          ccccaggacccacaggagccaccggagcACCCGGTCAAGCTGGAC‑CACCTGGAGCCACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGcc                                                               <  1:1860366/137‑1 (MQ=18)

                                                                             caggagcgacaggagcgacaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGTGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                                          <  2:1548075/110‑1 (MQ=255)

                                                                              aggagcgaccggagcgacaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAg                                                           >  2:1054943/1‑140 (MQ=255)

                                                                                   cgacaggagcgacaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCAACGGGGGAAGCCGGCCCAGCCGGAGCGAcc                                                      >  1:4470341/1‑139 (MQ=255)

                                                                                     ccaggagcgccaggcgcACCCGGACCACCAGGAC‑CCGCGGGAGCCACGGGAGCCACAGGAGATACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                    <  2:793385/139‑1 (MQ=17)

                                                                                     acaggagcgacaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGAc                                                                                                                 >  2:4653432/1‑79 (MQ=255)

                                                                                       tggagcaacaggagcTCCCGGGGAAGCTGGAC‑CAGCGGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                  <  1:4057191/139‑1 (MQ=255)

                                                                                       aggagctaaaggagcTCCCGGTCAACCTGGAC‑TAGCGGGAGTGATGGGAGCCACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCGACTGGAGCCACTGGCGATACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                  <  1:1727684/140‑1 (MQ=14)

                                                                                         gagcgacacgagcTACCGGCGAAGCTGGAC‑CACCTGCAGCCACGTGAGCGACAGGAGCTCCCG‑GGGAAGCGCGACCACCCGGTGCCACGGGAGCGACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCt                                                <  2:143021/140‑1 (MQ=18)

                                                                                          ggcgacaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                               >  1:4487107/2‑140 (MQ=255)

                                                                                          agcgacaggagcTCCCGGGGAAGCGGGAC‑CAGCTGGAGCGACGGGAGCGACCGGAGCGACCG‑GCGAAGCCGGACCAGCGGGGGCGACCGGAGCCACAGGGGCTCCGGGGGACGCCGGACCAGCCGGAGCGCCAGCAGCtc                                               >  2:747631/1‑139 (MQ=255)

                                                                                          agcgacaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCGACGGGAGCCACTGGAGCTACAGGGGAAGGCGCAGCTGCCTGAGCCTCAGGAGCTa                                               >  1:1088637/1‑140 (MQ=18)

                                                                                          agcgacaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                               <  1:3765434/140‑1 (MQ=255)

                                                                                          agcgacaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAG‑AACCGTGGGAAGTCGGACAAGCCGCGGCCACGGGAGCAACAGGAGCCACGGGTGAAGCCGGACCAGCCGCAGCGACAGGAGCTa                                               >  1:275027/1‑140 (MQ=18)

                                                                                            cgacaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCGACGGGAGCCACCGGGGCTACTGGGGAGGGGGGACCCGCCGGGGCGAGAGGAGCtctc                                             >  2:44918/1‑137 (MQ=18)

                                                                                            cgacaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGCGCGACGGGAGCGCCAGGGGCTACGGGGGACGCCGGCCCAGCCGGAGCGACAGGGGCTAcc                                             >  2:4039003/1‑140 (MQ=18)

                                                                                              acaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCACCCGGAGCGACAGGAGCTACCgg                                           >  1:716876/1‑140 (MQ=255)

                                                                                              acaggagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCCACAGGCGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGCGAACGCGGAGCACCCGGAGCCACAGCAGCGCCCgg                                           >  2:4752247/1‑140 (MQ=18)

                                                                                              acaggagcTACCGGGGAAGCTGGAC‑CAGCAGGAGCGACGGGAGCCACAGGAGCTACCG‑GGGAACCCTGACCACCCGCAGCCACCGGAGCAACAGGGGCTAGGGGGGACGCCGGACCAGCCGGAGCGACCGGCGCTCCCgg                                           >  1:1356743/1‑140 (MQ=18)

                                                                                                  gagcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGaa                                       >  2:1322936/1‑140 (MQ=255)

                                                                                                   agcTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCGACGGGAGCCACGGGCGCTACTGGCGATGAGGGACCTGCGGGAGCGACGGGAGATAcccgcgagg                                      >  2:4532938/1‑133 (MQ=17)

                                                                                                   agcTACCGGGGAAGCTGGAC‑CAGCGGGAGCGACGGGAGCGACAGGAGCAACCG‑GGGAAGCCGGACCACCCGGGGCCACGGGAGCGACAGGGGCGTCGGGCGAAGCGGGACCGGCCGGAGCGACGGGAGCTACCGGGGacg                                      >  2:1884788/1‑138 (MQ=18)

                                                                                                     cTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAg                                                                                               <  1:584894/81‑1 (MQ=255)

                                                                                                       aCCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGCGCTCCTGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGCGCAGCCgg                                  >  2:4920028/1‑140 (MQ=21)

                                                                                                       aCCGGCGAAGCTGGAG‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                                  <  1:3008253/140‑1 (MQ=255)

                                                                                                         cGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                <  1:1322936/140‑1 (MQ=255)

                                                                                                             gAAGCGGGAC‑CAGCGGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGGCGGACCAGCCGGAGCCACAGGCGCGACAGGGGCGACCGGCCCAGCCGGAGCGGCCGGAGCCAAGGGAGCGACCGGGGAAGCAGGACCAGc                            >  2:2325338/1‑140 (MQ=12)

                                                                                                              cctcTGGAC‑CAGCCGGAGCGACGGGACCCACAGGCGCTACCG‑GGGAACCCGGACATTCCGGGTCCTCTGGAGACTCCGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                           <  2:1565562/137‑1 (MQ=17)

                                                                                                                gcgggAC‑CAGCCGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGACGCGGGACCAGCCGGGGCCACGGGAGCCACAGGCGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                         <  1:4801660/137‑1 (MQ=18)

                                                                                                                ccTGGAC‑CTCGTGCAGCGACCGCACCTACAGGACCTACCG‑GGGAAGCCTGACCTTCCGTTGCCACGGGAGCCACAGGGGCACCTGGGGAAGCCGGAGCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                         <  2:1431955/139‑1 (MQ=14)

                                                                                                                  tGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGACGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGCCCCACCCGGAg                       >  2:983613/1‑140 (MQ=18)

                                                                                                                  tGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                                        >  1:3855426/1‑75 (MQ=255)

                                                                                                                  tGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACAGGGGAAGCCGGACCAGCCGTAg                       >  2:3120830/1‑140 (MQ=255)

                                                                                                                  tGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGCGCGCCGGGAGCCACAGGGGCGCCTGGGGACGCCGGACCAGCCGGAGCGCCAGGAGCTCCCGGGGACGCCGGACCAGCCggcg                       >  2:607173/1‑138 (MQ=17)

                                                                                                                  cggcc‑cAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGACGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                       <  2:561991/136‑1 (MQ=255)

                                                                                                                  cggAC‑CCTCTGGAGCGCCGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGCGCCACGGGTGCCACAGGCGCTTCTGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                       <  2:561845/139‑1 (MQ=17)

                                                                                                                  cggAC‑CAGCCGGAGCGACGGGAGCCACGGGAGCGACCG‑GTGAAGCGGGACCAGCCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                       <  2:2830459/139‑1 (MQ=11)

                                                                                                                  cggAC‑CACCCGGAGCCCCCGGAGCTACAGGCGATCCCG‑GACCACCCGGAGCCCCCGGAGCCACGGGAGCCACCGGTGCTACTGGCGCAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGATGCCGGACCAGCCGGAg                       <  2:3933685/139‑1 (MQ=2)

                                                                                                                   ggAC‑CAGCGGGAGCGACGGGAGCGACAGGCGCTACTG‑GGGAAGGCGGACCAGCCGGTGCCACGGGATCAACTGGGGCATCGGTGGACGCCGGACACGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAccggccggggg                      >  1:2146254/1‑137 (MQ=17)

                                                                                                                           cTGGAGCGATGGGAGCCCCAGGCGCTACCG‑GGGAACCCGGACCAGCCGGGGCCACAGGAGCCACAGGGCCTACTGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGAGCGACAggc               <  1:2914714/140‑2 (MQ=17)

                                                                                                                           cTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCACCCGGAGCGACAGGa               >  1:4386311/1‑140 (MQ=255)

                                                                                                                            tGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg              <  1:1119664/140‑1 (MQ=255)

                                                                                                                               aGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa           >  1:2556866/1‑140 (MQ=255)

                                                                                                                                  catgggAGCCACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCg        >  2:2597528/4‑140 (MQ=255)

                                                                                                                                   aCGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACggg       >  1:3958137/1‑140 (MQ=255)

                                                                                                                                    cGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                  <  2:3156245/96‑1 (MQ=255)

                                                                                                                                    cGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGCGCGACAGGGGACGACGGACCAGCCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGCACAGACAGGAGCGAGCggt      >  2:3900707/1‑139 (MQ=14)

                                                                                                                                     gggAGCGACAGGAGCTACCG‑GGGAATCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAACAGCCGGAGCGACAGGAGCTACCgggg     >  1:5055473/1‑140 (MQ=255)

                                                                                                                                     gggAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGACGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCggcg     >  2:4339681/1‑138 (MQ=255)

GGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGAC‑CAGCTGGAGCGACGGGAGCGACAGGAGCTACCG‑GGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAG  >  NZ_CP034943/607391‑607665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`