BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,214,518	T→G	8.3%	K60T (AAA→ACA)	nth ←	endonuclease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,214,518	0	T	G	8.3%	791.8 / 10.0	347	K60T (AAA→ACA)	nth	endonuclease III
Reads supporting (aligned to +/- strand): ref base T (77/225); new base G (33/12); total (110/237)
Fisher's exact test for biased strand distribution p-value = 8.08e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 371 total aligned reads displayed.

CACTTCTCCGCCGTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAT  >  NZ_CP034943/2214382‑2214657

cACTTCTCCGCCGTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTtat                                                                                                                                           >  2:4066493/1‑140 (MQ=255)

  cTTCTCCGCCGTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTata‑t                                                                                                                                        >  1:1096136/1‑140 (MQ=255)

  cTTCTCCGCCGTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTata‑t                                                                                                                                        >  1:4684671/1‑140 (MQ=255)

     ctcCGACGTAATCTTCAATAATCTTTTTGCTCAGTTTTTGGTTTTTTTTCGCTTTATTACGATACAAACCTATTGATTTAATATGCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTGCGGCCGTTTATA‑tttt                                                                                                                                     <  1:5048688/140‑1 (MQ=255)

          ccgTACTCTTCCAGCCTCCTTTTGCTCAGTTTTTGGAAGTGGTTCGCCTGCTTACGATACACACCGATTGATTTAATCTCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGaaa                                                                                                                                <  2:4771498/140‑1 (MQ=255)

            gTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAaata                                                                                                                              <  1:3729586/140‑1 (MQ=255)

            gTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAaata                                                                                                                              <  2:2482787/140‑1 (MQ=255)

             tAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAaataa                                                                                                                             >  2:3764479/1‑140 (MQ=255)

               aTCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATg                                                                                                                           >  2:2316513/1‑140 (MQ=255)

                  ttCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTTAAATAATGttt                                                                                                                        >  1:1619806/1‑140 (MQ=255)

                  ttCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCGTCCAGCGAAACAGCCACATAGTCTTCCGGCCGTTGATA‑TTTTTGAAATAATGttg                                                                                                                        >  2:2402285/1‑139 (MQ=255)

                   tCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGtttt                                                                                                                       <  2:5196570/140‑1 (MQ=255)

                       taatCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTc                                                                                                                   <  2:5025416/140‑1 (MQ=255)

                          tCATTTTGCTCAGTTTTTGGATGTTTTTCGGTTTATTACGATACAAACCGATTGATTTAAGATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTGGAAATAATGTTTTGGTCAct                                                                                                                <  2:1833588/140‑1 (MQ=255)

                          tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCAct                                                                                                                <  1:62066/140‑1 (MQ=255)

                          tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCAct                                                                                                                <  1:2164581/140‑1 (MQ=255)

                          tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCAct                                                                                                                <  1:876304/140‑1 (MQ=255)

                          tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCAct                                                                                                                <  1:2390444/140‑1 (MQ=255)

                          tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCAct                                                                                                                <  2:4318110/140‑1 (MQ=255)

                          tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCAct                                                                                                                <  1:4330718/140‑1 (MQ=255)

                          tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCAct                                                                                                                <  1:3641958/140‑1 (MQ=255)

                          tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGGTTATA‑TTTTTGAAATAATGTTTTGGTCAct                                                                                                                <  2:3839001/140‑1 (MQ=255)

                          acATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCAct                                                                                                                <  2:1231859/139‑1 (MQ=255)

                            aTTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCActct                                                                                                              <  1:4078622/140‑1 (MQ=255)

                              tttGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTTAAATAATGTTTTGGTAActaggt                                                                                                            >  2:4817226/1‑136 (MQ=255)

                                  cTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTAc                                                                                                        <  1:1008514/140‑1 (MQ=255)

                                     aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCTGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                     <  2:3895902/140‑1 (MQ=255)

                                     aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                     <  1:331495/140‑1 (MQ=255)

                                     aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                     <  2:2782636/140‑1 (MQ=255)

                                       tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTCATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTGATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                   <  1:4772520/140‑1 (MQ=255)

                                       tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCTAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                   <  2:3219733/140‑1 (MQ=255)

                                       tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGaaa                                                                                                                                <  1:1802657/111‑1 (MQ=255)

                                       tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                   <  1:2413671/140‑1 (MQ=255)

                                       tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                   <  1:3580364/140‑1 (MQ=255)

                                       tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                   <  1:4373515/140‑1 (MQ=255)

                                       tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                   <  1:1675924/140‑1 (MQ=255)

                                       tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                   <  2:448017/140‑1 (MQ=255)

                                            ggttttttttCGATTTATGACTCTACACACGTTTTGATTTAATATCCGGCTGAAGCTCTTCAAGCTAAACAGCCAATTTTTCTTCCGGCCTTTTATT‑TTTTTTAAATAATGTTTTTGTCACTCTGTTTACAAGTGCTTCt                                                                                              <  2:3623691/135‑1 (MQ=255)

                                                gTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTAc                                                                                          <  1:2207943/140‑1 (MQ=255)

                                                 tttttCTCTTTATTACGATACAAACCGATTTATTTAATATCCTGCTGAAGCTCTTCAAGCGAAACAGCCAAATAGTCTTCAGGCCGTTTATA‑TTTTTGAAAAAAAGGATTGGTCACTATGTTAACAATTGCATCTCTaaa                                                                                         >  2:3007654/1‑138 (MQ=255)

                                                 tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACa                                                                                         <  1:3271929/140‑1 (MQ=255)

                                                 tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAAAAATGTTTTTGTCACTCTGTTTACAAGTGCATCTGTACa                                                                                         >  2:396618/1‑140 (MQ=255)

                                                 tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTA‑TTTTTTTAATAATGTTTTTGTGACGCTGTTTTAAAGTGCAGATGTaaa                                                                                         >  1:2545398/1‑138 (MQ=255)

                                                 tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTA‑TTTTTTTAAAAATTTTTTGGTCACTATGTTTAAAAGTTGATCTTTACa                                                                                         >  1:310344/1‑140 (MQ=255)

                                                 tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTAAAAGTGCATCTGTaaa                                                                                         >  1:5235722/1‑138 (MQ=255)

                                                      cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                    <  2:4966627/140‑1 (MQ=255)

                                                      cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTGACAAGTGCATCTGTACATTgcg                                                                                    <  1:3008865/140‑1 (MQ=255)

                                                                   tACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTTGTCCCTCTTTTTTAAAATGCATATTTACAATTCGCAGATaaatagac                                                                       >  1:4626264/1‑135 (MQ=255)

                                                                   tACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAAAAATTTTTTTGTGAATCTTTTTTAAAGTGGATCTGTACATTTCGCAGATAAAACGAc                                                                       >  1:2267017/1‑140 (MQ=255)

                                                                   tACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATA‑TTTTTGAAATAATGTTTTTGTCACTCTGTTTTAAAGTGCATCTGTAGATTGCGCAGATAAAGCGAc                                                                       >  1:3988133/1‑140 (MQ=255)

                                                                        aCCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATATGTACATTGCGCAGATAAAGCGACTGCTa                                                                  >  2:2350777/1‑140 (MQ=255)

                                                                        aCCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTATGTTTAAAAGTGCATCTGTACATTTCGCAGATAAAACGACTGCTa                                                                  >  2:4860211/1‑140 (MQ=255)

                                                                        aCCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATA‑TTTTTGAAATAAAGTTTTGGTGACTCTGTTTTAAACTGCATCTGTAAATTGCGCAGATAAATCGACTGCTa                                                                  >  1:3912218/1‑140 (MQ=255)

                                                                            attgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTAAA‑ATTTTGAAATAATGTTTTTGTCACAAGGTTTAAAAGATCATCAGTAAAATTCGCAGATAAAGCGAAAGCAACCaa                                                              >  2:1677870/1‑139 (MQ=255)

                                                                             ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTTGTCACTCTGTTTAAAAGTGCATGTGTACAATTCGCACATAAAGCGAATGCTACCaat                                                             >  2:2092355/1‑138 (MQ=255)

                                                                             ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTTGTCACACTGTTTAAAAGTGCATCTGTACATTGCGCAGATAAACCGACTGCTAACaat                                                             >  2:2229596/1‑138 (MQ=255)

                                                                             ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTAc                                                                                                        >  2:2587317/1‑97 (MQ=255)

                                                                                    aaTATCCTGCTGAAGCTCTTCCAGCTAAACAGCCAAATAGTCTTCCGCCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTAAAAGTGCATCTGTACATTGCGCCGATAAAGCGACTGCTACCACTAATTCaa                                                      <  1:1035395/140‑1 (MQ=255)

                                                                                    aaTATCCTGCTGAAGCTCTTCCAGCGAAACAGCAAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCaa                                                      <  1:1312059/140‑1 (MQ=255)

                                                                                     atatCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCaaa                                                     <  1:2350777/140‑1 (MQ=255)

                                                                                          ctgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCAGTTTATA‑TTTTTTAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                <  1:2005153/140‑1 (MQ=255)

                                                                                          aggctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                <  2:4909933/138‑1 (MQ=255)

                                                                                          aggctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                <  2:3544382/138‑1 (MQ=255)

                                                                                           tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTTTA‑TTTTTGTAATAATGTTTTTGTGACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAACGACTGCTACCACAAAATCAAAAGGAtt                                               >  1:1225009/1‑140 (MQ=255)

                                                                                                   ctctTCCCGCGAAACAGCCAAATAGTCTTCCGGCCGTTAATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                       <  1:5173417/140‑1 (MQ=255)

                                                                                                   ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCTTTTATT‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                       <  2:2161503/140‑1 (MQ=255)

                                                                                                   ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                       <  2:4684671/140‑1 (MQ=255)

                                                                                                   ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                       <  1:3822317/140‑1 (MQ=255)

                                                                                                   ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                       <  1:3533352/140‑1 (MQ=255)

                                                                                                   ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                       <  2:1619806/140‑1 (MQ=255)

                                                                                                   ctctTCCAGCGAAACACCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTCCATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                       <  1:266969/140‑1 (MQ=255)

                                                                                                   ctctGCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                       <  1:4560072/140‑1 (MQ=255)

                                                                                                     ctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                     <  1:4897902/140‑1 (MQ=255)

                                                                                                     ctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGGACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                     <  2:63372/140‑1 (MQ=255)

                                                                                                     ctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTA‑TTTTTGGAATAATGTTTTGGTGACTCTGGTTACAACTGCATCTGTACAGTGGGCAGATAAAACGACTGCGACCCCGAAATAAAAACGATTTGGATGAAcc                                     >  1:1427219/1‑140 (MQ=255)

                                                                                                       tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTTAAAAAATG‑TTTGGTCCCACTGTTTTGAACTGCATCTGTAGAATTGGCAGATAAAACGACTGCTAACACAAAATCCAAATGAGTTGGATGAAcccg                                   >  1:714867/1‑138 (MQ=255)

                                                                                                       tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTTCGCAGATAAAGCGACTGCTACCACTAATTAAAAAGGATTGGAATTAACCat                                   >  2:2446820/1‑139 (MQ=255)

                                                                                                       tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATA‑TTTTTGTAATAATGTTTTGGTCACGCTGTTTTCAAGTGCATCTTTACATTGCGCAGATAAAGCGACTGCTAACACAAATTCAAAAGGATTTGAATTAACCAg                                   >  2:3298625/1‑140 (MQ=255)

                                                                                                       tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACAAATTAAAAAGGCTTGGAATGAAcccg                                   >  2:87814/1‑138 (MQ=255)

                                                                                                           gagAAACAGCCAAATAGTCTTCCGGCCTTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTca                               <  1:4936902/138‑1 (MQ=255)

                                                                                                              aaaCAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCacac                            <  2:4526462/140‑1 (MQ=255)

                                                                                                                aCAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACCGCTACCAc                                                              <  1:2788817/104‑1 (MQ=255)

                                                                                                                   gCCCAATAGTCGTCCGGCCGTTGATC‑TTTCTGAAATAATGTTTTGGTCACTCTGTTGACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGc                       <  2:602840/140‑1 (MQ=255)

                                                                                                                          aGTCTTCCGGCCGTTTATA‑TTTTGGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGa                <  2:1003451/140‑1 (MQ=255)

                                                                                                                            tCTTCCGGCCGTTTATA‑TTTTTTGAATAATGTTTTGGTAACTCTGTTTAAAAGTGCATCTGTTAATTGGGCATATAAAGCGACTGCAAACCCTAAATAAAAACGAATTGAATGAACCACTTAAACCGCTGCGGGTGGaaa              >  2:3074882/1‑140 (MQ=255)

                                                                                                                            tCTTCCGGCCGTTTATA‑TTTTTTAAATAATGTTTTGGTCACACTGTTTACAAGTGCATCTGTACATTTCGCAGATAAACCGACTGCTACCACTAAATAAAAAGGAGTGGAATTAACCAGTTCAACCTCTGCGTGTGGaaa              >  2:281611/1‑140 (MQ=255)

                                                                                                                              ttCCGGCCGTTTATA‑TTTTTTAAAAAATGTTTTTGTCAATATGTTTAAAAGTGCAACTGTACATTTCGCATATAAATCGACAGATAACAAAAATTAAAAAGTAATGGAATGAAACAGTTAACACACTGCGTGTGGTAACa            >  1:3764856/1‑140 (MQ=255)

                                                                                                                              ttCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTGACTGTGTTTACAAGTGCATCTGTAAATTGCGCAGATAAATCCACTGCTACCACAAAATCAAAAGGATTGGAATGAAACAGTTAACACGCTGCCTGTGGAAACa            >  2:2464329/1‑140 (MQ=255)

                                                                                                                              ttCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACa            <  1:5013438/140‑1 (MQ=255)

                                                                                                                              ttCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACa            <  1:4817226/140‑1 (MQ=255)

                                                                                                                               tCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACAt           <  2:1106421/140‑1 (MQ=255)

                                                                                                                                    ccGTTTTTA‑TTTTTTAATTAATGTTTTGGTCACTCTGTTTAAAAGTGAATCTGTACATTGCGCAGAGAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGc      <  1:2976103/140‑1 (MQ=255)

                                                                                                                                        ttATT‑TTTGTGAAATAATGTTTTGGTCACTCTGTTTAAAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt  <  1:3420667/140‑1 (MQ=255)

                                                                                                                                        ttATA‑TTTTTTAAATAATGTTTTTGTCACTCTGTTTACAAGTGAATCTTTACATTGCGCAGATAAAGCGACTGCTACCAAGAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt  <  1:4457472/140‑1 (MQ=255)

                                                                                                                                        ttATA‑TTTTTGAAATAATGTTTTGGTCACTCTTTTTACAAGTTAATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTCCGTGTGGAAACATGTCGCCTAt  <  2:1136141/140‑1 (MQ=255)

                                                                                                                                        ttATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt  <  1:917761/140‑1 (MQ=255)

                                                                                                                                        ttATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt  <  2:1399107/140‑1 (MQ=255)

                                                                                                                                        ttATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt  <  2:1990564/140‑1 (MQ=255)

                                                                                                                                        ttATA‑TTGTTGAAATTATGTCTGGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt  <  2:3110744/140‑1 (MQ=255)

                                                                                                                                        ttATA‑TATTTCAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt  <  1:4062778/140‑1 (MQ=255)

CACTTCTCCGCCGTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAT  >  NZ_CP034943/2214382‑2214657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`