BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,214,555	C→A	8.1%	V48L (GTA→TTA)	nth ←	endonuclease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,214,555	0	C	A	8.1%	762.9 / 6.0	306	V48L (GTA→TTA)	nth	endonuclease III
Reads supporting (aligned to +/- strand): ref base C (64/202); new base A (32/7); total (97/209)
Fisher's exact test for biased strand distribution p-value = 3.32e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 309 total aligned reads displayed.

CTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAATTTGTTTTAGATTTA  >  NZ_CP034943/2214416‑2214692

cTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTAc                                                                                                                                           <  1:1008514/140‑1 (MQ=255)

   cgTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                                        <  2:2296807/139‑1 (MQ=255)

   cgTTTGTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTGGGTCACTCTCTTTACAAg                                                                                                                                        <  2:3366441/139‑1 (MQ=255)

   aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                                        <  2:2475306/140‑1 (MQ=255)

   aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                                        <  1:1468407/140‑1 (MQ=255)

   aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                                        <  2:2782636/140‑1 (MQ=255)

   aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                                        <  1:4596417/140‑1 (MQ=255)

   aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAACCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                                        <  1:4766978/140‑1 (MQ=255)

   aGTTTTAGGATGTTTTTCGCTTTATTACGATACAAACCGATTTATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                                        <  2:3675157/140‑1 (MQ=255)

   aGTTGTTGGATGTTTTTCGCGTTATTACGCTACAAACCGAGTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGGTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                                        <  2:627773/140‑1 (MQ=255)

     tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGACGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGATTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  1:2408859/140‑1 (MQ=255)

     tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  1:2255716/140‑1 (MQ=255)

     tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  1:649552/140‑1 (MQ=255)

     tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  2:3274465/140‑1 (MQ=255)

     tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  1:3580364/140‑1 (MQ=255)

     tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  1:1345920/140‑1 (MQ=255)

     tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  1:4064138/140‑1 (MQ=255)

     tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  2:4438924/140‑1 (MQ=255)

     attttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTGATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                      <  1:40384/139‑1 (MQ=255)

     attttGGATGTTTGTCGCTTTATTACGATACAAAACGATTGATTTAATATCCTGATGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCTGACGTTTATA‑ATTTTGAAATAATGTTTTGCTCACTCTGTTTACAAGTg                                                                                                                                      <  2:3862775/139‑1 (MQ=255)

          tgATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCt                                                                                                                                 <  2:1266847/139‑1 (MQ=255)

          ggATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCt                                                                                                                                 <  1:379773/140‑1 (MQ=255)

               tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCTAAACAGCCAAATAGTCTTCCGGCCGTGTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTTCATATGTaaa                                                                                                                            >  1:4122552/1‑138 (MQ=255)

               tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACa                                                                                                                            <  1:3271929/140‑1 (MQ=255)

               tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACa                                                                                                                            <  2:3094477/140‑1 (MQ=255)

               tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAAAAATGTTTTTGTCACTCTGTTTACAAGTGCATCTGTACa                                                                                                                            >  2:396618/1‑140 (MQ=255)

               tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTA‑TTTTTTTAATAATGTTTTTGTGACGCTGTTTTAAAGTGCAGATGTaaa                                                                                                                            >  1:2545398/1‑138 (MQ=255)

               tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTA‑TTTTTTTAAAAATTTTTTGGTCACTATGTTTAAAAGTTGATCTTTACa                                                                                                                            >  1:310344/1‑140 (MQ=255)

               tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTAAAAGTGCATCTGTaaa                                                                                                                            >  1:5235722/1‑138 (MQ=255)

                    cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                                                       <  1:2340742/140‑1 (MQ=255)

                    cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                                                       <  2:4487950/140‑1 (MQ=255)

                    cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTGACAAGTGCATCTGTACATTgcg                                                                                                                       <  1:3008865/140‑1 (MQ=255)

                    cGCTGTAGTACGATACAAACCGATTGATTTAATATCCTGCGGAAGCTCTTCCCGCGAAACAGCCAAATAGTCTTCCGGCCGAGTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                                                       <  1:3301712/140‑1 (MQ=255)

                                aaaCAAACCGTTTTATTTAATATCCTGCTTAAGCTCTTCCAGCTAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGa                                                                                                           <  1:910578/138‑1 (MQ=255)

                                 tACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTTTA‑TTTTTTTAATAATTTTTTTGTGACTCTGTTTTAAATTTCATCTGTACATTGCGCAGATAAAGCGAc                                                                                                          >  1:98269/1‑140 (MQ=255)

                                 tACAAACCGAGTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCACATAGTCTTCCGGCCGTGTATA‑TTTTTTAAATTAGGTTTTGGTGACTCTGTTTACAATTGCATCTGTACAATGCGCAGATAAAGCGAc                                                                                                          >  1:4773102/1‑140 (MQ=255)

                                   cAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTg                                                                                                        >  2:1120570/1‑140 (MQ=255)

                                      aCCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATATGTACATTGCGCAGATAAAGCGACTGCTa                                                                                                     >  2:2350777/1‑140 (MQ=255)

                                      aCCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTATGTTTAAAAGTGCATCTGTACATTTCGCAGATAAAACGACTGCTa                                                                                                     >  2:4860211/1‑140 (MQ=255)

                                         gattgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATATGTACATTGCGCAGATAAAGCGACTGCTAACa                                                                                                  >  2:2083724/1‑140 (MQ=255)

                                          attgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATA‑TTTTTGAAAAAATGTTTTGGTCACTCTGTGTAAAAGTGCATCTTTAAAATTCGCAGATAAACCGACTGCTACCaa                                                                                                 >  2:2194752/1‑139 (MQ=255)

                                           ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTTGTCACACTGTTTAAAAGTGCATCTGTACATTGCGCAGATAAACCGACTGCTAACaat                                                                                                >  2:2229596/1‑138 (MQ=255)

                                           ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATA‑TTTTTTAAATAATGTTTTGGTCACGCGGTTTACAACTTCATCTTTACATTTCGCAGATAAAGCGCCTGCTAcacaa                                                                                                >  1:1990564/1‑136 (MQ=255)

                                                  aaTATCCTGCTGAAGCTCTTCCAGCTAAACAGCCAAATAGTCTTCCGCCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTAAAAGTGCATCTGTACATTGCGCCGATAAAGCGACTGCTACCACTAATTCaa                                                                                         <  1:1035395/140‑1 (MQ=255)

                                                  aaTATACTGCTGAACCTCTTCCAGCGAACCAGAGAAATAGTCTCCCGGCCGTTAATA‑TTTTTGAAATAATGTTTTGGACACTATTTTTACAACTTCATCTGTACATTGCTAAGATAAAGCGACTGCTACCACTAATTCaa                                                                                         <  1:4638924/140‑1 (MQ=255)

                                                       ccTGCTGAAGCTCGTCCCGCGAACCAGCCAAATAGTCTTCCGGCCGTGTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTCCATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGa                                                                                    <  2:4982953/140‑1 (MQ=255)

                                                        ctgctgAAGCTCTTCCAGCGACACAGCCAAATCTTCTTCCGGCCGTGTATA‑TTTTTTAAATAAAGTTGTGGTGACGCTGTTTTAAAGTGCTACTGTCGATTGAGCAGGTAAAGCGACTGCGACCCCGACATCGAAAGGAt                                                                                   >  2:1085843/1‑140 (MQ=255)

                                                        ctgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATA‑TTTTTGAAATAATTTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                                                   >  2:2702731/1‑140 (MQ=255)

                                                        ctgctcAAGCTCGTCCAGCGAAACAGCCAAATAGCCTTCCGGCCCTTTATA‑TGTTTGAAATAATGTTTCGGTCACTCTGTTTAAACGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                                                   <  1:1082504/140‑1 (MQ=255)

                                                        ctgcagAAGCACTTCCCGAGAAACAGCCAAATCGTCTTCCGGCCGTTTATA‑TTGTAGAAATCATGTTTTGGTCACTCTGTTTACAAGTGCATCTGGACATTGCGCAGATACAGCGACTGCTACCACTAATTCCAAAGGAt                                                                                   <  1:4369996/140‑1 (MQ=255)

                                                        aggctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                                                   <  2:4909933/138‑1 (MQ=255)

                                                         tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATA‑TTTTTGAAATAATGTTTTTGTGACTCTGTTTTCAAGTGCCTCTGTACATTGCGCAGATAAAGCGACTGCTACCACAAAATAAAAAGGAtt                                                                                  >  1:278563/1‑140 (MQ=255)

                                                                 ctctTCCAGCGCCACAGCCACAGAGTCTTCCGGCCGTTTATA‑TTTTGCAAATAATGTTTTGGTCACTCTGTTCACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                                          <  1:4860211/140‑1 (MQ=255)

                                                                 ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                                          <  2:1619806/140‑1 (MQ=255)

                                                                 ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                                          <  2:2545398/140‑1 (MQ=255)

                                                                 ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                                          <  1:3533352/140‑1 (MQ=255)

                                                                 ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                                          <  1:3670316/140‑1 (MQ=255)

                                                                 ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                                          <  2:4684671/140‑1 (MQ=255)

                                                                 ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                                          <  1:5108505/140‑1 (MQ=255)

                                                                 ctctTCCAGCGAAACACCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTCCATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                                          <  1:266969/140‑1 (MQ=255)

                                                                 ctctGCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                                          <  1:4560072/140‑1 (MQ=255)

                                                                   gttCCAGCTAAACAGACAAATAGTCTTCCGGCCTTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTTTACATTGCGCATATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                                                        <  1:1835557/139‑1 (MQ=255)

                                                                   ctTCCCGCGAAACAGCAAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                                                        <  1:5205676/140‑1 (MQ=255)

                                                                   ctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                                                        <  1:1294832/140‑1 (MQ=255)

                                                                     tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTTAAAAAATG‑TTTGGTCCCACTGTTTTGAACTGCATCTGTAGAATTGGCAGATAAAACGACTGCTAACACAAAATCCAAATGAGTTGGATGAAcccg                                                                      >  1:714867/1‑138 (MQ=255)

                                                                     tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTTCGCAGATAAAGCGACTGCTACCACTAATTAAAAAGGATTGGAATTAACCat                                                                      >  2:2446820/1‑139 (MQ=255)

                                                                     tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                                                        <  1:3618145/138‑1 (MQ=255)

                                                                     tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTA‑TTTTTTGAATAAAGTTTTGGTCACTCTGGTTAAAAGTGCATCTGTACATTTCGCAGATAAATCGACTGGTAACACAAATTCAAAAGGATTTGAATTaaacct                                                                      >  1:4909933/1‑136 (MQ=255)

                                                                     tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACAAATTAAAAAGGCTTGGAATGAAcccg                                                                      >  2:87814/1‑138 (MQ=255)

                                                                       cAGCGAAACATCCAAATAGTGTTCCGGCCGTTAATA‑TTTTTTAAATAATGTTTTGGTCAATCTGTTTACAAGTGCATCTGTACATTGCGCCGTTAAAGCGACTGATACCACTAATTCAAAAGGATTGGAATGAACCAGtt                                                                    <  1:160443/140‑1 (MQ=255)

                                                                         gagAAACAGCCAAATAGTCTTCCGGCCTTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTca                                                                  <  1:4936902/138‑1 (MQ=255)

                                                                            aaaCAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCacac                                                               <  2:4526462/140‑1 (MQ=255)

                                                                              aCAGCCCAATAGTCTTCCGGCCGTGTATA‑TTTTTGGAATAATGTTTTGGGCACTCTGGTTACAAGTGCATCTGTACAGTTCGCAGATAAAGCGACTGCTACCACTCAGTGAAAAGGATTGGAATGAACCCGTTCACActc                                                             >  2:141819/1‑140 (MQ=255)

                                                                                 gCCCAATAGTCGTCCGGCCGTTGATC‑TTTCTGAAATAATGTTTTGGTCACTCTGTTGACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGc                                                          <  2:602840/140‑1 (MQ=255)

                                                                                          tCTTCCGGCCGTTTATA‑TTTTTTAAATAATGTTTTGGTCACACTGTTTACAAGTGCATCTGTACATTTCGCAGATAAACCGACTGCTACCACTAAATAAAAAGGAGTGGAATTAACCAGTTCAACCTCTGCGTGTGGaaa                                                 >  2:281611/1‑140 (MQ=255)

                                                                                            ttCCGGCCGTTTATA‑TTTTTTAAATAATGTTTTTGTCACTCTGTTTAAAATTTCATCTGTACAATGCGCAGATAAAACGACTGCTACCACTAATTCAAAAGGATTTGAAAGAAACAGTTAACACTCTCCGTGTGGAAACa                                               >  2:4045598/1‑140 (MQ=255)

                                                                                            ttCCGGCCGTTTATA‑TTTTTTAAATAATGTTTTGGTCACTGGGTTTACAAGTGCATCTGTAACTTGCGCAGATAAAGCGACTGCTACCACAAATTCAAAAGGATTGGAATTTACCAGTTAACAATCTGCGGTTGGAAACa                                               >  2:3015931/1‑140 (MQ=255)

                                                                                            ttCCGGCCGTTTATA‑TTTTTTAAAAAATGTTTTTGTCAATATGTTTAAAAGTGCAACTGTACATTTCGCATATAAATCGACAGATAACAAAAATTAAAAAGTAATGGAATGAAACAGTTAACACACTGCGTGTGGTAACa                                               >  1:3764856/1‑140 (MQ=255)

                                                                                            ttCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTTGTCACTCTGTTTACAAGTGCATATGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTAAAAAGGATTTGAATGAACCAGTTAAAACTCTTCGTGTGGAAACa                                               >  2:2417566/1‑140 (MQ=255)

                                                                                             tCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCCTCTGTCCATTGCGCAGATAAAGCGACTGCTACCACTAAGTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACAt                                              <  2:1871896/140‑1 (MQ=255)

                                                                                             tCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACAt                                              <  2:5096312/140‑1 (MQ=255)

                                                                                                  ccGTTTTTA‑TTTTTTAATTAATGTTTTGGTCACTCTGTTTAAAAGTGAATCTGTACATTGCGCAGAGAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGc                                         <  1:2976103/140‑1 (MQ=255)

                                                                                                  ccGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGc                                         <  1:387133/140‑1 (MQ=255)

                                                                                                  ccGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGc                                         <  2:528709/140‑1 (MQ=255)

                                                                                                  ccGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGc                                         <  2:2990712/140‑1 (MQ=255)

                                                                                                  ccGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGc                                         <  2:4825158/140‑1 (MQ=255)

                                                                                                    gTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCt                                       <  2:3912218/140‑1 (MQ=255)

                                                                                                      ttATT‑TTTGTGAAATAATGTTTTGGTCACTCTGTTTAAAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt                                     <  1:3420667/140‑1 (MQ=255)

                                                                                                      ttATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt                                     <  2:1990564/140‑1 (MQ=255)

                                                                                                      ttATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt                                     <  1:213167/140‑1 (MQ=255)

                                                                                                      ttATA‑TTTTTGAAATAATGTTTGGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt                                     <  1:396618/140‑1 (MQ=255)

                                                                                                       tata‑TTTTTGAAATAATGTTTTTGTCACTCTGTTTAAAAGTGCATCTGTACATTTCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAACACTTAAAAATCTGCGTGTGGAAAAATGTCGCCTAta                                    >  2:3602105/1‑139 (MQ=255)

                                                                                                              tttGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCt                              <  2:3111463/140‑1 (MQ=255)

                                                                                                                       atGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAAt                     >  1:1185164/1‑140 (MQ=255)

                                                                                                                            ttGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAAt                <  2:4122552/140‑1 (MQ=255)

                                                                                                                                    tctGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAATTTGTTTTa        >  2:193889/1‑140 (MQ=255)

                                                                                                                                    tctGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAATTTGTTTTa        >  1:20555/1‑140 (MQ=255)

                                                                                                                                    tctGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAATTTGTTTTa        >  2:3537627/1‑140 (MQ=255)

                                                                                                                                        tttACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAATTTGTTTTAGAtt    >  2:71601/1‑140 (MQ=255)

                                                                                                                                          tACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAATTTGTTTTAGATTTa  >  1:4988212/1‑140 (MQ=255)

CTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATA‑TTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAATTTGTTTTAGATTTA  >  NZ_CP034943/2214416‑2214692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`