BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,991,262	G→T	16.9%	intergenic (+63/‑144)	gbsR → / → bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,991,262	0	G	T	16.9%	248.9 / 18.1	128	intergenic (+63/‑144)	gbsR/bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand): ref base G (25/76); new base T (21/2); total (50/78)
Fisher's exact test for biased strand distribution p-value = 3.47e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

GATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAGGGGGAATT  >  NZ_CP034943/2991125‑2991400

gcttGGCTGAAACGGCTTGCTGAATCGGTGCAGCGCGGTGAAATTTTTAAATTTATACCGATTCAAACAAAGTAACCCAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGaa                                                                                                                                          <  2:3390144/138‑1 (MQ=255)

 aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGaaa                                                                                                                                         >  2:2940447/1‑140 (MQ=255)

 aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTG‑TTTTTTTTTTTG‑TAATTAAC‑AA‑AAAT‑TTTaaa                                                                                                                                         >  1:2636492/1‑140 (MQ=255)

 aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTG‑TTGTTTTTTATG‑TAATTAAT‑AC‑AAAA‑TTGtaa                                                                                                                                         >  2:1076711/1‑140 (MQ=255)

  ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTG‑TTTTTTTTTATT‑TTATTAAT‑AC‑AAAT‑TTGtaaa                                                                                                                                        >  1:4738496/1‑140 (MQ=255)

    ggCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTATTTTTTATT‑TAATTAAT‑AC‑AAAT‑TTTAAatat                                                                                                                                      >  2:4148455/1‑140 (MQ=255)

     gCTTAAACGGCTTGCTGACTCGGTGCAGAGCGGGTAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATa                                                                                                                                     <  2:4984738/140‑1 (MQ=255)

     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTT‑TTGTTTTTTATT‑TAATTACT‑AA‑AAAT‑TTTAAAAATa                                                                                                                                     >  2:3891878/1‑140 (MQ=255)

     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTG‑TTATTTTTTAAT‑TAATTAAA‑AC‑AAAA‑TTGaaaaaaa                                                                                                                                     >  2:304310/1‑138 (MQ=255)

     ccTGAAACGGCTTGCTGAATCGGTGCAGACCGGGGAAATTTTTAAATTTATACCGATTGAAACAAACTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATa                                                                                                                                     <  2:4106711/139‑1 (MQ=255)

            cGGATTGCTCAATCGGTCCAGAGCGGGGAATTTTTTAATTTTATACCGATTGAAAAAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGaaa                                                                                                                              <  1:2796784/140‑1 (MQ=255)

               cTTGCTGAATCGGTGCAGAGCGGGGACATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAACCCGGGCGTGTTGTTT‑TTAGTTTGTTTT‑GAGTTAAT‑AC‑ACAT‑TTGAAAAATACGGGGAGAAc                                                                                                                           >  1:2587538/1‑140 (MQ=255)

               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAATACAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTGTTTTTTTTG‑TAATTACA‑AC‑AAAA‑TTGAAAAATACGGGAAAAAc                                                                                                                           >  2:63923/1‑140 (MQ=255)

               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTT‑TTGTTTTTTTTT‑TTTTTAGA‑AG‑AAAA‑TTTAAAAAAAAAGGaaaaaa                                                                                                                           >  1:2004133/1‑139 (MQ=255)

                        tCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCAttt                                                                                                                  <  2:16469/140‑1 (MQ=255)

                        tCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTT‑TTGTTTTTTATT‑TAATTAAT‑AC‑AAAA‑TTGAAAAAAAAGGGAAAAAAATACCagat                                                                                                                  >  1:1082850/1‑137 (MQ=255)

                         cGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGAGTTTTTTTG‑TTATTTTTTATT‑TAATTAAA‑ACAAAAT‑TTAAAAAATA‑GGGAAAAAAATAACATTTa                                                                                                                 >  2:2689396/1‑140 (MQ=255)

                          ggTGCAGAGCGGGTAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTAc                                                                                                                <  2:3912342/140‑1 (MQ=255)

                            tGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAATCGCCTGGGAAACCGGGCGTGTTTTTG‑TTGTTTTTTGTG‑TGATTGCT‑TC‑ACAG‑TTTTAAAATAAGGGACAATCATCGCAgtttagt                                                                                                              >  1:3527571/1‑136 (MQ=255)

                            tGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTT‑TTCTTTTTTAAT‑TAAATACT‑AC‑AAAT‑TTGAAAAAAAAGGGAAAAAAAACCCATTTACtt                                                                                                              >  2:1345226/1‑140 (MQ=255)

                            tGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTG‑TTGTTTTTTTTGTTATTTATA‑AA‑AAAT‑TTTAAAAAAAGGGAAAAAAAAGCCCGTTTatg                                                                                                               >  1:4381194/1‑138 (MQ=255)

                            tGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTG‑GTTTTTTTTTTT‑TTATTAAA‑AA‑AAAA‑TTTTAAAATAAGGGAAAAAAATCCCaggttatt                                                                                                              >  1:4722614/1‑133 (MQ=255)

                            tGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTGTTTG‑TTTTTTTTTTTT‑TTATTACT‑ACAAAAA‑TTTAAAAAAAGGGGAAAAAAACCCAAttaatt                                                                                                               >  1:289678/1‑136 (MQ=255)

                               agagCGGGTAATTTTTTAAATTTATACCGATTGAAAAAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCa                                                                                                           <  2:4374794/140‑1 (MQ=255)

                                gagCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTGTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGCAAAAGATGCCATTTACTTccca                                                                                                          >  2:4511521/1‑138 (MQ=255)

                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAATCGCCTGGGAAACCGGGCGTGTGTTTG‑TTTGTTTTTTTG‑TTATTAAT‑AC‑AAATATTATAAAATA‑GGGTCAAAAAACCCAATTAATTCCAAAAAAtt                                                                                                    >  1:648439/1‑140 (MQ=255)

                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTGTTTTTTATG‑TAATTAAT‑AA‑AAAA‑TTGTAAAATACGGGAAAAAAATCCCAGTTACTTCCAAAATAtt                                                                                                    >  2:3527046/1‑140 (MQ=255)

                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATAtt                                                                                                    <  2:714240/140‑1 (MQ=255)

                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTT‑TTGTTTTTTTTT‑TTATTACA‑AC‑AAAA‑TTTAAAAAAAAGGGAAAAAAAACACCATTACTTGCAAAataat                                                                                                    >  1:3517684/1‑138 (MQ=255)

                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTTTTT‑TAATTA‑T‑AA‑AAAA‑TTTATAAATAAGGGGAAAACAAACCAATTAGTTCAAAAAAAtt                                                                                                    >  1:577759/1‑140 (MQ=255)

                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTG‑TTGTTTTTTTTT‑TAATTAAG‑AC‑AAAT‑TTGAAAAATACGGGACAAACATAACCTTTACTTACAAAATAtt                                                                                                    >  2:3179834/1‑140 (MQ=255)

                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTG‑TTGGTTTTTATG‑TAAATACT‑AC‑CAAT‑TTGGAAAATTCGGGGAAAACCTGCCAGTTAGTTGCAAAATAtt                                                                                                    >  2:4421892/1‑140 (MQ=255)

                                           tttttAACTTTATACCGCTGGAAACCAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTGTTG‑TGGTTTTTTATG‑TAATTTCT‑AA‑AAAT‑TTTCATATTAACGGAAATAAGTCCCATTTACTTCACAAATATTTGATg                                                                                               >  2:1514423/1‑140 (MQ=255)

                                           tttttAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTG‑TTTTTTTTTATG‑TTATTACT‑AA‑AAAA‑TTTAAAAAAACGGGGAAAAAATCCCATTTTATTCCAAAAAATTTGATg                                                                                               >  1:3911773/1‑140 (MQ=255)

                                           tttttAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTG‑TTGTTTTTTATT‑TAATTAAA‑AC‑AAAA‑TTTAAAAAAACGGGAAAAACAAACCAGTTAATTACAAAAAATTTGATg                                                                                               >  2:1854011/1‑140 (MQ=255)

                                            ttttAATTTTATCCCGATTTATCAAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTTAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                                              <  2:790332/140‑1 (MQ=255)

                                            ttttAAATTTATCCCGATTGAAACAAAGTAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                                              <  1:244019/140‑1 (MQ=255)

                                            ttttAAATTTATACCGATTTAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                                              <  1:3937997/140‑1 (MQ=255)

                                            ttttAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTATTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                                              <  2:703055/140‑1 (MQ=255)

                                            ttttAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCAGGGAAACCGGGCGTTTTATTG‑TTCTTTTTTATG‑TAATTACG‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                                              <  1:1703131/140‑1 (MQ=255)

                                            ttttAAATTTATACCGATTCAAAAAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                                              <  1:3387637/140‑1 (MQ=255)

                                            tttaAAATTTACACCGATTCAAACAAACAAAACCAAAACCGCCTGGGAAACCGGGCGTTTTTTTG‑ATCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                                              <  1:4137335/140‑1 (MQ=255)

                                                     tataCCGATTGAACCAAAGTAAACCAACACCGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTAt                                                                                     <  1:3944449/140‑1 (MQ=255)

                                                     aaaaCCGATACAAACAAACAAAACCAAAAACGCCAGGTAAACCGGGCGTTATATTG‑ATCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTAt                                                                                     <  1:2973445/137‑1 (MQ=255)

                                                           gATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                                               <  2:441386/140‑1 (MQ=255)

                                                           gATTGAAAAAAACTAAAACAAAACCGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                                               <  2:3060092/140‑1 (MQ=255)

                                                           gATTCAAACAAAGTAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                                               <  2:1691522/140‑1 (MQ=255)

                                                            aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTa                                                                              <  1:274446/140‑1 (MQ=255)

                                                            aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTATTTTTTATG‑TAATTACT‑AA‑AAAT‑TTTAAAAATACGGGCAAAAAATCCCATTTACTTCCAAAATTATTTTTTGTAATTTTAtgtatgta                                                                              >  2:4863910/1‑140 (MQ=255)

                                                            aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTT‑TTGTTTTTTTTT‑TTATTAAT‑AC‑AAAA‑TTTTAAAAAACGGGAAAAACATCACATTTTATTGCAAAATATTTGATTGTAATTTTATTTAGTTa                                                                              >  2:2071147/1‑140 (MQ=255)

                                                            aTTGAAACAAACTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTa                                                                              <  2:3353052/140‑1 (MQ=255)

                                                             tagAAACAAAGTAAACCAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTaa                                                                             <  1:881989/138‑1 (MQ=255)

                                                                aaaCACAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                                          <  2:2271570/140‑1 (MQ=255)

                                                                aaaCAAATTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                                          <  1:1392676/140‑1 (MQ=255)

                                                                aaaCAAAGTAACACAAAATCGGATTGGAAACCGGGCTTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AA‑AAAT‑TTGAAAAATACGGTTAAAACATCCCATTTAATTCCAATATATTTTATGGTAATTTTATGTAGGTAAAAt                                                                          <  1:5035528/140‑1 (MQ=255)

                                                                aaaCAAAGTAAAACACACACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAACCATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                                          <  2:5134047/140‑1 (MQ=255)

                                                                aaaCAAAGTAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAACAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                                          <  2:677886/140‑1 (MQ=255)

                                                                      aGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTT‑TTGTTTTTTATT‑TAATTACT‑AC‑AAAT‑TTTTAAAATAAGGGAAAAAAATACCATTTACTTCCAAAATATTTTTTTGTAAGTTTATGTATGTAAAATTTTTAt                                                                    >  2:2127334/1‑140 (MQ=255)

                                                                      aGTAAAACAAAAACGCCTGGGAAACCGGGCGTTGTTTTG‑TTCTTTTTTATT‑TAATTAATAAC‑AAAT‑TT‑AAAAATAACGGAAAAAAAACCCAGTTTGTGCCAAAATATTTGAGTGTAATTTTGTGTATGTAAAATTTTTag                                                                    >  2:4684673/1‑139 (MQ=255)

                                                                      aGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTG‑TTGTTTTTTATT‑TAATTAAT‑AA‑AAAT‑TTTAAAAAAACGGGAAAAAAAACCCATTTAATTCCAAAAAATTTGATTGTTATTTTATGTAGGTAAAATTTTTAt                                                                    >  2:4173842/1‑140 (MQ=255)

                                                                         aaaaCAAAAACGCCTGGGAAACC‑GGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                                <  1:2489090/140‑1 (MQ=255)

                                                                          aaccAAACACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                                <  1:4896893/137‑1 (MQ=255)

                                                                          aaacaaacACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGACAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                                <  1:1302567/140‑1 (MQ=255)

                                                                          aaacaaacACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                                <  1:2127334/140‑1 (MQ=255)

                                                                          aaacaaacACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                                <  1:510311/140‑1 (MQ=255)

                                                                          aaaaaaaaCCGCCTGCGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                                <  1:3849468/140‑1 (MQ=255)

                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAACACATCCCATGTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                                <  2:4566805/140‑1 (MQ=255)

                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                                <  2:2680407/140‑1 (MQ=255)

                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                                <  1:2940447/140‑1 (MQ=255)

                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                                <  1:3891878/140‑1 (MQ=255)

                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                                <  2:1199902/140‑1 (MQ=255)

                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                                <  1:2986186/140‑1 (MQ=255)

                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                                <  1:990808/140‑1 (MQ=255)

                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                                <  2:403643/140‑1 (MQ=255)

                                                                           aaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGc                                                               <  2:568013/140‑1 (MQ=255)

                                                                           aaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGc                                                               <  2:5030640/140‑1 (MQ=255)

                                                                           aaCAAAAACGCCTGGGAAACCGGGCGTGTTTTTG‑TTGGTTTTTATG‑TAATTAAG‑AC‑AAAA‑TTTAAAAATAAGGGAAAAACAACCCCTTTACTTCCAAAAAATTTGTTTGTAATTTTATGTAGGTAGAAATTTTAttttgg                                                               >  2:109614/1‑137 (MQ=255)

                                                                                    gCCTGGGACACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                                      <  1:3378017/140‑1 (MQ=255)

                                                                                    gCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTGGGTAAAATTTGTATTTGGCCGTTCagag                                                      <  1:4385401/140‑1 (MQ=255)

                                                                                    gCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                                      <  2:174321/140‑1 (MQ=255)

                                                                                    gCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                                      <  2:3878796/140‑1 (MQ=255)

                                                                                                   cGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                                       <  1:5110679/140‑1 (MQ=255)

                                                                                                   cGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                                       <  1:4826190/140‑1 (MQ=255)

                                                                                                   cGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                                       <  2:4349311/140‑1 (MQ=255)

                                                                                                        ttttG‑TTCTTTTTTAGG‑TAATTACT‑AC‑AACT‑TAGAAAAATACGCGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGc                                  <  2:1629211/140‑1 (MQ=255)

                                                                                                              ttCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGaa                             <  2:1231703/140‑1 (MQ=255)

                                                                                                               tcatttttATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAat                            <  1:3680463/137‑1 (MQ=255)

                                                                                                                 ttttttATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAatat                          >  1:1123478/1‑140 (MQ=255)

                                                                                                                        tG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTtata                   <  2:1062060/140‑1 (MQ=255)

                                                                                                                        tG‑TAATTAAT‑AC‑AAAT‑TTGAATAATTCGGGAAAAACATCCCATTTACTTCCAAAATATTTTATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTtata                   <  2:651784/140‑1 (MQ=255)

                                                                                                                              ttACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCCGGCCTGAATATTTTTATAACttt              >  1:2199492/1‑140 (MQ=255)

                                                                                                                                act‑ac‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACttttt            <  1:252543/140‑1 (MQ=255)

                                                                                                                                    ac‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAgg         <  2:2303916/140‑1 (MQ=255)

                                                                                                                                    ac‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAgg         <  2:1146660/140‑1 (MQ=255)

                                                                                                                                       aaaT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAgggg       <  1:3446678/140‑1 (MQ=255)

                                                                                                                                       aaaT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAgggg       <  1:2525519/140‑1 (MQ=255)

                                                                                                                                       aaaT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAgggg       <  1:1883948/140‑1 (MQ=255)

                                                                                                                                       aaaT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAgggg       <  1:1297575/140‑1 (MQ=255)

                                                                                                                                          t‑ttGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAGGGGGaa    <  2:2631182/140‑1 (MQ=255)

                                                                                                                                             tGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAGGGGGAAtt  >  2:2169321/1‑140 (MQ=255)

GATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTG‑TTCTTTTTTATG‑TAATTACT‑AC‑AAAT‑TTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAGGGGGAATT  >  NZ_CP034943/2991125‑2991400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`