BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,139,479	C→G	7.0%	S40C (TCT→TGT)	pucL →	urate oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,139,479	0	C	G	7.0%	814.7 / 3.4	334	S40C (TCT→TGT)	pucL	urate oxidase
Reads supporting (aligned to +/- strand): ref base C (95/195); new base G (27/2); total (137/197)
Fisher's exact test for biased strand distribution p-value = 1.41e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 346 total aligned reads displayed.

ACGTAAAGGAAGATGCCAATGTTCACAATGGATGACATGAACCAAATGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGAACAAAGAATACAATGAGCGTCACCTCGGTAAGAGAGCAGCA  >  NZ_CP034943/3139343‑3139617

acttAAAGGAAGATGCAAATTTTCAAACTGGATGACATTAACCATATGGACATTAAAAGACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTTCGGAGAAAGCCGCGGCATTGCGGCCGTTttcttc                                                                                                                                          <  2:766018/137‑1 (MQ=255)

 cGTAAAGTAAGATGCCAATGTTCACAATGGATGACATGAACCAAATGGACATTCAAACACTGACAGACACGATTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTcc                                                                                                                                         <  1:648621/140‑1 (MQ=255)

  gTAAAGGAAGATGCCAATGTTCACAATGGATGACATGAACCAAATGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTccc                                                                                                                                        <  1:4433837/140‑1 (MQ=255)

     aaGGAAGATGCCAATGTTCACAATGGATGACATGAACCAAATGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTAt                                                                                                                                     <  2:4010241/140‑1 (MQ=255)

          aGATGCCAATGTTCACAATGGATGACATGAACCAAATGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGAt                                                                                                                                >  1:3510489/1‑140 (MQ=255)

              gCCAATGTTCACAATGGATGACATGAACCAAATGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTGTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTc                                                                                                                            <  2:4376953/140‑1 (MQ=255)

                 caggTTCACAATGGATGAAATGAACCAAATGGACATTCAAACACGGACAGACACGCGTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTcacc                                                                                                                         <  1:3766108/137‑1 (MQ=255)

                  aTGTTCACAATGGATGACATGAACCAAATGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTACCTATCTGATCTTcacca                                                                                                                        >  1:5107427/1‑140 (MQ=255)

                    gTTCACAATGGATGACATGAACCAAATGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACcaca                                                                                                                      <  1:1777636/140‑1 (MQ=255)

                      tCACAATGGATGACATGAACCAAATGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACaaa                                                                                                                    <  1:3652370/140‑1 (MQ=255)

                       cacaATGGATGACATGAACCAAATGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACaaaa                                                                                                                   >  1:4768159/1‑140 (MQ=255)

                       cacaATGGATGACATGAACCAAATGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACaaaa                                                                                                                   >  1:4962812/1‑140 (MQ=255)

                       cacaATGGATGACATGAACCAAATGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATATTCACCACaaaa                                                                                                                   >  2:2072461/1‑140 (MQ=255)

                          aaTGGATGACATGAACCAAATGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTTTTCCCTATCTGATATTCAACACAAAATgg                                                                                                                >  1:4289582/1‑140 (MQ=255)

                           aTGGATGACATGAACCAAATGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGc                                                                                                               <  1:849986/140‑1 (MQ=255)

                               aTGACATGAACCAAATTGACATTCAAACACTGACAGACACGATTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGc                                                                                                           <  2:3152455/140‑1 (MQ=255)

                               aTGACATGAACCAAATGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGc                                                                                                           <  1:5209683/140‑1 (MQ=255)

                               aTGACATGAACCAAATGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGc                                                                                                           <  1:2819968/140‑1 (MQ=255)

                               aTGACATGAACCAAATGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTATTCCCTATCTGATCTTCACCACAAAATGGCCAGc                                                                                                           >  1:3194755/1‑140 (MQ=255)

                               aTGACATGAAACAAATGGACATTCAAACACTGACAGACACTCTTTAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGc                                                                                                           <  2:3007048/140‑1 (MQ=255)

                                      gAACCAAATGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTaa                                                                                                   >  2:1367159/1‑140 (MQ=255)

                                      gAACCAAATGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTaa                                                                                                   <  1:2708503/140‑1 (MQ=255)

                                      gAACCAAATGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTaa                                                                                                   <  1:288540/140‑1 (MQ=255)

                                      gAACCAAATGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTaa                                                                                                   <  1:163226/140‑1 (MQ=255)

                                        aCCAAATGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCGATCTGATATTCACCACAAAATGGCCAGC‑AGTGTaaaa                                                                                                 >  1:2549298/1‑140 (MQ=255)

                                            aaTGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGAGTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCcg                                                                                             <  2:1638865/140‑1 (MQ=255)

                                             aTGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGATAGCCGCGGCATTGCGGCCGGTTTCTTACCTCTCTGATCTTCACCACACACTGGCCCGC‑AGTGTCAAAGCcgc                                                                                            >  2:4114632/1‑140 (MQ=255)

                                             aTGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCCGC‑AGTGTAAAAGCcgc                                                                                            >  2:617838/1‑140 (MQ=255)

                                              tgcaCATTCAAACACTGACAGACTCGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCcgcg                                                                                           <  1:4185014/137‑1 (MQ=255)

                                              tGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCcgcg                                                                                           >  2:3903463/1‑140 (MQ=255)

                                              tGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCcgcg                                                                                           <  1:4370158/140‑1 (MQ=255)

                                              tGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCcgcg                                                                                           <  2:2882303/140‑1 (MQ=255)

                                                gaaaTTCAAACACTGAGAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGa                                                                                         <  2:3280144/137‑1 (MQ=255)

                                                gACATTCAAAGACTGACAGACTCGATTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCTGCATTGCGGCCGTTTTCTTCCCTTTCTGATCTTCTCCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGa                                                                                         <  2:2584944/140‑1 (MQ=255)

                                                        aaCACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGa                                                                                 <  1:186961/140‑1 (MQ=255)

                                                        aaCACTGACAGACACGATTGAATCTATTTTTGAACACTCTTCTTGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGa                                                                                 <  2:1541854/140‑1 (MQ=255)

                                                          cacTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGaca                                                                               <  2:1237938/140‑1 (MQ=255)

                                                              gacagacaCGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGc                                                                           <  1:4812719/140‑1 (MQ=255)

                                                              gacagacaCGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGc                                                                           <  1:686171/140‑1 (MQ=255)

                                                               acagacaCGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑CTTGTAAAAGCCGCGGACCGCCAGACCCAGCt                                                                          >  2:4405520/1‑140 (MQ=255)

                                                               acagacaCGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTGTTGGTGCCGATCTGATGTTCACCACAAAAATGCCAGC‑CATTTAAAATCCGCGGACCGCCCGACACAACt                                                                          >  1:2162243/1‑140 (MQ=255)

                                                               acagacaCGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTGTTCTTGCCAAAAAGATATTAACCACAAAAATGCCAAA‑AATGTAAAAAACGCGGACAGCCACACACAGca                                                                          >  1:773012/1‑139 (MQ=255)

                                                               acagacaCGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTGTTCTTCCCTATCTGGTATTCAACACAAAATGGGCAGC‑ATTTTAAAAACCGCGGACCGCCCCACACAGCt                                                                          >  1:4675037/1‑140 (MQ=255)

                                                               acagacaCGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTGTTCTTCCCGAGCTGATCTTCAACACAAAAATGCCCCC‑AATGTAAAAGCCGCGGACCGCCACACACAGCt                                                                          >  1:4000733/1‑140 (MQ=255)

                                                                     acGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGAttt                                                                    <  2:4743826/140‑1 (MQ=255)

                                                                      cGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTCTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTa                                                                   <  2:879569/140‑1 (MQ=255)

                                                                        cTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGaca                                                                               >  1:4612632/1‑126 (MQ=255)

                                                                        cTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGaca                                                                               <  2:4612632/126‑1 (MQ=255)

                                                                              tCTATTTTTTAAATCTCTTGATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACATAATGGCCAGC‑ATTGTAAAAGCCGCGGAGCGCCAGACACAGCTTGATTTTATcaacaa                                                           <  2:3805691/140‑1 (MQ=255)

                                                                              tCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTGTTACCTATCTGGACTTAACCCCAAAAATGCCACC‑CATTTAAAAGCCGCGGACCCCCCGACACAGCTTTATTTAATaaacaa                                                           >  1:1434136/1‑140 (MQ=255)

                                                                              tCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATcaacaa                                                           <  2:4818827/140‑1 (MQ=255)

                                                                                tATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGc                                                         <  2:23911/140‑1 (MQ=255)

                                                                                 aTTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTGTTGCCGATCTGATTTTGAACACAAAATTGCCAGC‑ATTGTAAAAGCCGCGGAACGCCAGAACAACCTTGATTTAATCAACAAGCa                                                        >  1:4509137/1‑140 (MQ=255)

                                                                                 aTTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTGTTACCTATATGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCACCCAGCa                                                        >  2:127883/1‑140 (MQ=255)

                                                                                 aTTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTCTCTGATCTTCACCACAAAATGGCCAGC‑AAGGTAAAACCCGCGGACCGCCCGACACAGCAGGATTTAAACAACAAGcc                                                        >  1:1564581/1‑139 (MQ=255)

                                                                                 aTTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCa                                                        >  2:4928140/1‑140 (MQ=255)

                                                                                 aTTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAAATGCCAGC‑AATCTAAAAGCCGCGGACCCCCAGACACAGCTTGATATAATCCACAAGCa                                                        >  2:3968712/1‑140 (MQ=255)

                                                                                 aTTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTAACGGATCTTCACCACAAAATGGCCACC‑ATTGTAAAAGCCGCGGACCCCCCGACACAGCTTGATTTAAGCAACAAGcc                                                        >  1:5008318/1‑139 (MQ=255)

                                                                                   ttttGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCAcc                                                      <  2:4724935/140‑1 (MQ=255)

                                                                                          cacTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACt                                               <  1:470049/140‑1 (MQ=255)

                                                                                            ctctTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGc                                                                                                               <  1:1022696/75‑1 (MQ=255)

                                                                                            ctctTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCACC‑ATTTTAAAAGCCGCGGACCCCCAGACACAGCTTGATTTAATCAACAAACACCCCCCACTCg                                             >  2:2036392/1‑140 (MQ=255)

                                                                                             tattCATGGATTGCGGATAAATCATCTGAATTGCGTAATTTTTAATACCTATATTATATTCACCAAAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCgg                                            >  2:3533823/3‑140 (MQ=255)

                                                                                              ctTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGa                                           <  1:1088270/140‑1 (MQ=255)

                                                                                              ctTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGa                                           <  1:3052802/140‑1 (MQ=255)

                                                                                              ctTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGATTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGa                                           <  1:2054861/140‑1 (MQ=255)

                                                                                               ttCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTGTTGCCAATATGATATTGAACACAAAATTGGCAGC‑ATTGTAAAAACCGCCGGCCACCCGCCACCCCGTTATTTAATAAAAAAACACCCCCCACACgcca                                          >  1:3105385/1‑137 (MQ=255)

                                                                                                 cATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGAACa                                        <  1:2238309/140‑1 (MQ=255)

                                                                                                  aTGGATTGCGGAGAAAGCCGCGGCATTGCGGCCTTTTTCTTCCCTATCTGATCTTCAACACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGAACaa                                       <  2:4528530/140‑1 (MQ=255)

                                                                                                    ggATTGAGGAGAAAGCCGCGGCCTTGAGGGCGGTTTCTTCCCTATCTTATCTACACCACAATATGGCCAGC‑ATTGTAAAACCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAATCACCCCCCACTCGGAACAAAg                                     <  1:3707262/140‑1 (MQ=255)

                                                                                                      aTTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAATATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGAACAAAGaa                                   <  2:3450710/140‑1 (MQ=255)

                                                                                                      aTTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGAACAAAGaa                                   <  1:3159131/140‑1 (MQ=255)

                                                                                                      aTTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGAACAAAGaa                                   <  1:2139744/140‑1 (MQ=255)

                                                                                                      aTTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGAACAAAGaa                                   <  1:1452476/140‑1 (MQ=255)

                                                                                                       ttGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAACCCGCGGACCGCCCGACACACCTTGATTTAATCAACAAGCACCCCCCACTCGGAACAAAGaaa                                  >  1:3626947/1‑139 (MQ=255)

                                                                                                       ttGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAAAGGCCCGC‑ATTGTAAAAGCCGCGGACCGCCACACACAGCTTGATTTAATCAAAAAGCACCCCCCACTCGGAACAAAGaaa                                  >  2:1888367/1‑139 (MQ=255)

                                                                                                       ttGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTATTGCCTATCTGATCTTCACCAAAAAATGGACAGC‑AATGTAAAATCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGAACAAACAAt                                  >  2:4241532/1‑140 (MQ=255)

                                                                                                       ttGCGGAGAAAGCCGCGGCATTGCGGCCGTGTTCTTCCCGATATGATCTTCACCACAAAATGGCCACC‑CTTGTAAAAGCCCCGGACCGCCCGACACAGCTTGATTTAATCAACAAGCACCCCCCACGCGGAACaaactat                                  >  1:1501687/1‑136 (MQ=255)

                                                                                                        tGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTTTTACCGAAATCATATTAACCACAAAAAGGCCCGCAATTTTAAAAACC‑CCGACCCCCCCACACCCCTTTAATTTATAAAAAAACAACCCCCCCCCCGAAAAAACAATa                                 >  1:1247054/1‑140 (MQ=255)

                                                                                                        tGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGAACAAAGAATa                                 <  2:3217955/140‑1 (MQ=255)

                                                                                                         gcctagaAAGCCGCGGCATTGCGGCAGTTTTCGTCCCTAAGTGATCTTCACCAAAAAATGGGGAGC‑ATTGTAAAATCCGAGGTCCGCCAGACACACCTTGATTTAATAAAAAAGCAACCCCCCCTAGGAAAAAAGAAAAc                                >  2:1956987/5‑140 (MQ=255)

                                                                                                         gCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTcc                                                                                                                                         <  1:3302517/36‑1 (MQ=255)

                                                                                                         gCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTcc                                                                                                                                         >  2:3302517/1‑36 (MQ=255)

                                                                                                         gCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGa                                           >  2:921476/1‑129 (MQ=255)

                                                                                                         gCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGa                                           <  1:921761/129‑1 (MQ=255)

                                                                                                         gCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACACGGAACAAAGAATAc                                >  2:3699728/1‑140 (MQ=255)

                                                                                                          cGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGAACAAAGAATACa                               <  2:4289582/140‑1 (MQ=255)

                                                                                                                 aGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGATTGATTTAATCAACAAGCACCCCCCACTCGGAACAAAGAATACAATGAGCg                        <  2:2258085/140‑1 (MQ=255)

                                                                                                                   ccGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGAACAAAGAATACAATGAGCGTc                      <  1:2470494/140‑1 (MQ=255)

                                                                                                                   ccGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGAACAAAGAATACAATGAGCGTc                      <  1:4118024/140‑1 (MQ=255)

                                                                                                                   ccGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGAACAAAGAATACAATGAGCGTc                      <  2:3397914/140‑1 (MQ=255)

                                                                                                                    cgcgGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCCGC‑ATTGTAAAATCCGCCGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGAACAAAGAAAACAATGAGCGTCa                     >  2:4566237/1‑140 (MQ=255)

                                                                                                                    cgcgGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCA‑CCCCCACTCGGAACAAAGAATACaa                              <  1:1891795/130‑1 (MQ=255)

                                                                                                                     gcgGCATTGGGGCGGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGAACAAAGAATACAATGAGCGTCAc                    <  2:2731972/140‑1 (MQ=255)

                                                                                                                        gCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGAACAAAGAATACAATGAGCGTCACCTc                 <  2:5040548/140‑1 (MQ=255)

                                                                                                                        gCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGAACAAAGAATACAATGAGCGTCACCTc                 <  2:674782/140‑1 (MQ=255)

                                                                                                                             gCGGCCGTTTTCTTCCCTATCTGATCTTCAACACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGAACAAAGAATACAATGAGCGTCACCTCGGTaa            <  2:1441704/140‑1 (MQ=255)

                                                                                                                              cGGCCGTTTTGTTCCCGATCTGATCGTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGTGGTCCGCCAGACCCAGCGTTATTTAATCAACCACCACCCCCCCCTCGGAACAAAGAATACAATGAGCGTCCCCGCGGGAag           >  2:1772164/1‑140 (MQ=255)

                                                                                                                                       tcttcCCTATCTGATCTTCACCACAAACTGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGAACAAAGAATACAATGAGCGTCACCTCGGTAAGAGAgcagca  <  1:2341251/140‑1 (MQ=255)

                                                                                                                                       tcttcCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGAACAAAGAATACAATGAGCGTCACCTCGGTAAGAGAgcagca  <  1:2484969/140‑1 (MQ=255)

ACGTAAAGGAAGATGCCAATGTTCACAATGGATGACATGAACCAAATGGACATTCAAACACTGACAGACACGCTTGAATCTATTTTTGAACACTCTTCATGGATTGCGGAGAAAGCCGCGGCATTGCGGCCGTTTTCTTCCCTATCTGATCTTCACCACAAAATGGCCAGC‑ATTGTAAAAGCCGCGGACCGCCAGACACAGCTTGATTTAATCAACAAGCACCCCCCACTCGGAACAAAGAATACAATGAGCGTCACCTCGGTAAGAGAGCAGCA  >  NZ_CP034943/3139343‑3139617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`