BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,254	G→C	5.3%	L41V (CTT→GTT)	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,254	0	G	C	5.3%	643.3 / 6.1	322	L41V (CTT→GTT)	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (216/75); new base C (3/20); total (226/96)
Fisher's exact test for biased strand distribution p-value = 6.82e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 337 total aligned reads displayed.

CTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAG  >  NZ_CP034943/3851115‑3851393

ctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAg                                                                                                                                              <  2:3953379/140‑1 (MQ=255)

  ctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGcc                                                                                                                                            <  1:479172/140‑1 (MQ=255)

   tGTAATATGGATTGGACATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                                           >  1:3478317/1‑140 (MQ=255)

   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGACCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                                           >  1:3933764/1‑140 (MQ=255)

   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                                           >  2:4435976/1‑140 (MQ=255)

   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                                           >  2:1784443/1‑140 (MQ=255)

   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGGTATTACCTGTATCATGATTCGATCGAATGCAAGccc                                                                                                                                           >  1:3805553/1‑140 (MQ=255)

    gTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCt                                                                                                                                          >  2:2863928/1‑140 (MQ=255)

        tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTccc                                                                                                                                      >  1:357121/1‑140 (MQ=255)

        tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTccc                                                                                                                                      >  1:664173/1‑140 (MQ=255)

           ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                                                                   <  1:3144880/140‑1 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATATCCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGGTATTTGCTGTATCATGATTCGGTCGGATGGTAGCCGTTccccacaa                                                                                                                                 >  1:2922987/1‑135 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAATCCCTTCCCaaaaa                                                                                                                                 >  2:723545/1‑140 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                 >  1:1991026/1‑140 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                 >  1:803609/1‑140 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                 >  1:3025843/1‑140 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCATCCCaaaaa                                                                                                                                 >  1:1350711/1‑140 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGCAAGCCCTTCCCaaaaa                                                                                                                                 >  1:2277441/1‑140 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                 >  1:1916004/1‑140 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAGTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                 >  1:4881078/1‑140 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACTGTATCATGATTCGATAGTATTCAAGACCTTCACaaaaa                                                                                                                                 >  1:1489237/1‑140 (MQ=255)

               tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACg                                                                                                                               >  2:2854523/1‑140 (MQ=255)

               tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACg                                                                                                                               >  1:2546777/1‑140 (MQ=255)

               tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACg                                                                                                                               >  2:1234223/1‑140 (MQ=255)

               tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACg                                                                                                                               >  2:3453843/1‑140 (MQ=255)

               tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATCGTAAGCAAGCCCGTCCCAAAAACg                                                                                                                               >  2:4627736/1‑140 (MQ=255)

                 gAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑At                                                                                                                            >  2:2042558/1‑140 (MQ=255)

                 gAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑At                                                                                                                            >  2:1632089/1‑140 (MQ=255)

                             tctTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAATGATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTg                                                                                                                >  1:1753113/1‑140 (MQ=255)

                                  tCGCTAGTTTTATGACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGGCTTCTATCAATGACCCAGGGGTGGTTACCTGTCTCATGATTGGATCGTATGCCAGGCCGTGCCACACTCG‑ATAACACGACTCTgggtct                                                                                                           >  1:2733153/1‑135 (MQ=255)

                                  tCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTGcttat                                                                                                           >  1:997645/1‑138 (MQ=255)

                                  tCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAAG‑ATAAAACGACTCTGcttct                                                                                                           >  1:4360006/1‑140 (MQ=255)

                                         tttaATCACATTTTAATCATAACATTAAAAGTACTCGCACAGTTCTTATATAAATCAACCATCCTTTATTACCTGTATCATTATTCGATCTTATCCAACCCCTTCCAAAAAACC‑ATAAAACGACTCTGCTTCTTCAAGtt                                                                                                    <  2:4194652/140‑1 (MQ=255)

                                           ttATAAACTTTTAATCATACCTTTAAAATTACTGGTACTGTTGTTCTATAATTCAACCAGTCGTTATTCCCTGTCTAATGATTCGTTGGTATGCACGCCCTTCCCAAACACG‑ATAACACGACTCTGCTTCTTCAAGTTCg                                                                                                  <  2:891439/140‑1 (MQ=255)

                                             aTCACGTTGTAAGAATAACATTAAAAGTACTCGGACAGGTGTTAAAAAAATCAACCAGTCGTTTTTACCTGTATATTGTTTCGCTCGTCTGCACGCCTGCCCAAAAAACT‑AAAACACGACTCTGCTTCTTCAAGTTCGCa                                                                                                <  2:3221373/140‑1 (MQ=255)

                                                  cTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACa                                                                                           >  2:5147915/1‑140 (MQ=255)

                                                  cTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACa                                                                                           >  2:2421875/1‑140 (MQ=255)

                                                  cTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAAAGAATAGATATTAAGCAAGCCCGTACCAAAAACG‑AAAAAAAGACACTTCTTCTTAAAGTTCTCAAcaca                                                                                           >  1:4749686/1‑140 (MQ=255)

                                                   ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAAG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAt                                                                                          >  1:1325101/1‑140 (MQ=255)

                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTCTTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACCACTCTGCTTCTTCAAGTTCGCAAGACAAAACAAATtg                                                                                 >  1:4482206/1‑140 (MQ=255)

                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACCTAACAACTtg                                                                                 >  1:864170/1‑140 (MQ=255)

                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                 >  1:1502773/1‑140 (MQ=255)

                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                 >  2:1245411/1‑140 (MQ=255)

                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                 >  1:2890963/1‑140 (MQ=255)

                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                 >  1:681303/1‑140 (MQ=255)

                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                 >  1:1801965/1‑140 (MQ=255)

                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTGCTTATTCAAGTTCGCAAGACATAACAACTtg                                                                                 >  1:1195567/1‑140 (MQ=255)

                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATAGTATTCAAGCCCATCCCAAAAACG‑ATAAAAATACTCTTCTTATTCAATTTCGCAATACATaacaacaat                                                                                 >  2:4853851/1‑137 (MQ=255)

                                                               ccTTCATTGTACCCGTACAGGTCTTCTATAAAGAAACCAGCGTTTATTCCCTGTATCATTCTTCGATCGTATCAAAGCCCTTCCCAAAAACG‑ATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:329179/140‑1 (MQ=255)

                                                               ccTTAAAATTACTGGGACCGGTCTTCTATAAATGAACCAGTGTTTATTACCTGTATATTGATTCGATCGTATGAAAGCCCTTCCAAAAAACG‑AAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:4360006/140‑1 (MQ=255)

                                                               ccTGCAACGTCCTCGGAGAGGTCTTATATCAATGACCCAGTCTTTATTACGTTTAGCTTGATACGATCTTCTGCACCCCCTGCCACAAACCG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:3074811/140‑1 (MQ=255)

                                                                    aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCACGCCCTTCCCAAAAACG‑ATAAAACGACACTGCGTCTTCAAGTTCGCAAGACCTAACAACTTGATGAGGCt                                                                         >  2:2176253/1‑140 (MQ=255)

                                                                    aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCt                                                                         >  2:3876336/1‑140 (MQ=255)

                                                                    aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAAC‑ATAAAACCACTCAGCTTATTCCATTTCGCAAGACAAAAAAACTTGATGAGGCt                                                                         >  1:759618/1‑140 (MQ=255)

                                                                          ctgggACTGGTATTCTTTAAATTAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                                   <  2:3647977/137‑1 (MQ=255)

                                                                          cTCGGACCGGTCTTCTATAAATCAACCCTTCTTTATTACCTGTATCATTTTTCGATCGTATGCAAGCCCTTCCCAAAAACC‑ATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                                   <  2:4533505/140‑1 (MQ=255)

                                                                          cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTGCCAAAAACG‑ATAAAACGACTCTGCTTCTTGAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                                   >  1:1126864/1‑140 (MQ=255)

                                                                          cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                                   >  2:803627/1‑140 (MQ=255)

                                                                          cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                                   >  2:190943/1‑140 (MQ=255)

                                                                          cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCAGTATCATGATTCGAACGTAAGCAAGCCCTTCCCAAAAACG‑ATAAAAACACTATGCTTCTTCAATTTAGCAAGACAAAACAACTTGATGAGGCTAAagag                                                                   >  1:2243321/1‑140 (MQ=255)

                                                                              tagtgttcttttATAAATTACCCATTCTTTATTACTTGTATAATGTTTCTATGGTTGGCACCCCCTCCCCAAAACCC‑AAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                                               <  1:4185815/134‑1 (MQ=255)

                                                                              gACAGTTATTATATAAATCAACCCGTGTTTATTACCTTTATAATTATTCTTTCGTATGAAACCCCTTCCAAAAAACG‑AAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                                               <  1:983649/140‑1 (MQ=255)

                                                                              caCAGGTTTTCTATAAATTAACCATTGTTTATTACCTTTATCATTATTCTATCTTATGCAAGCCCTTCCCAAAACAG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                                               <  2:2251730/139‑1 (MQ=255)

                                                                              caCAGGACTACTATAAATCAAGCCGTCGTTATTACAATTACACTGATTCGATCTGATGAAAGCCCTTCCCAAAAAAC‑ATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                                               <  1:5090730/139‑1 (MQ=255)

                                                                                  ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATg                                                           >  2:1966341/1‑140 (MQ=255)

                                                                                  ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGCAAGCCCTTCCCAAAAACC‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATg                                                           >  2:15086/1‑140 (MQ=255)

                                                                                  ggTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCCAAAACG‑ATAAAACGACTCTGCTTATTCAAGTTCGCAAGACATAAAAACTTGATGAGGCTTAAGAGCGATCATg                                                           >  2:2377271/1‑140 (MQ=255)

                                                                                       tatataAATCAACCATTGTTTTTTACCTGTATCTTGTTTCGATCGTATGAAAGCCCTTCCAAAAAACT‑AAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                                                      <  2:696131/138‑1 (MQ=255)

                                                                                             aaTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAACACCACTCTGCTTCTTCAAGTTCGCAAGACCTAACAACTTGATGTGGCATAAGAGCGATCATGTATAAATTTcc                                                >  2:3707660/1‑140 (MQ=255)

                                                                                             aaTGAACCAGTCGATATTACCGGGATCAGGATTCGATCGTATGCAAGCCCGTCACAAAAACG‑AAAAAACGACACTGCTTCTTCAAGGTCGCAAGACCTAACCACTTGATGAGGGTTAAGACCGGTCAGGTCTAAATTGcc                                                >  2:2352330/1‑140 (MQ=255)

                                                                                              aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCGTCCCAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAAATTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCg                                               >  1:5047012/1‑140 (MQ=255)

                                                                                              aTGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTGCTTGTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTTCCg                                               >  1:2086845/1‑140 (MQ=255)

                                                                                                 acccAGTCTTTATTACCTGTAACATGATTCGATCGTTGGAAAGCCCTACCCAAAAACG‑ATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                            <  2:4574993/138‑1 (MQ=255)

                                                                                                 acccAGTCGTTTTTACCGTTAACATTTTTCGATCGTATCCACCCCCTCCCAAAAAACC‑AAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAGTTGCCGATa                                            <  1:3324313/138‑1 (MQ=255)

                                                                                                 aaCCTGTCGTTATTACCTTTATCATGATTCGATCGTCTGCAAGCCCTTCCAAAAAACT‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                            <  2:2469403/140‑1 (MQ=255)

                                                                                                   ccATTCTTTATTCCCTGTAACATGCTTCGATCGTAGTCACGCCCTCCCCAAAAACG‑ATACCACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                                          <  2:1417303/140‑1 (MQ=255)

                                                                                                   acAGTCTTTTTTCCCTTTACCATGAGTCGCTGTTAGGCACGCCCTTCCAAAAACCC‑AAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                                          <  1:2063278/139‑1 (MQ=255)

                                                                                                     aGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGc                                        >  1:3186761/1‑140 (MQ=255)

                                                                                                        cGTTATTACGTGTATCTTTATTCGATGGTATCCAAGCCGTACCCAAAAACT‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTta                                     <  2:4743063/140‑1 (MQ=255)

                                                                                                          ttattaCCTGTATAATGATTCTATCGTATGCACGCCCTTCCAAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTtata                                   <  2:1077511/140‑1 (MQ=255)

                                                                                                              taCCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTAGCAAGACATAACAACTTTATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCgg                               >  1:412059/1‑140 (MQ=255)

                                                                                                                  tGTATATTGATTCGATCTTCTCAAAGCCCTTCACAAAAACC‑AAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAt                           <  1:4059799/140‑1 (MQ=255)

                                                                                                                   ttttaaTGTTTCGTTCGTCGGCAAGCCCTCCCAAAAAACG‑ATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAtt                          <  1:4540619/135‑1 (MQ=255)

                                                                                                                   ttATCATTTTTCGTTCGTCGGCACGCCCTCCCAAAAAACC‑AAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAtt                          <  1:220778/139‑1 (MQ=255)

                                                                                                                    tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt                         >  1:2694155/1‑140 (MQ=255)

                                                                                                                           aTTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                  >  2:2810071/1‑140 (MQ=255)

                                                                                                                           aTTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                  >  1:3422876/1‑140 (MQ=255)

                                                                                                                              cttttttATGAAAGCCCTTCCAAAAAACT‑ATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGCTATTTCCATAAAATc               <  1:1002356/134‑1 (MQ=255)

                                                                                                                                aTCTTATGCAAGCCCTTCCCAAAAACT‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCtt             <  2:1350711/140‑1 (MQ=255)

                                                                                                                                aTCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAACATCtt             >  1:4965866/1‑140 (MQ=255)

                                                                                                                                aTCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCtt             >  2:2940372/1‑140 (MQ=255)

                                                                                                                                   ttATGCAAGCCCTTCCAAAAAACT‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTccc          <  2:269278/139‑1 (MQ=255)

                                                                                                                                    tATGCACCCCCTT‑CCAAAAACGTAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACGTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTcccc         <  1:1075229/140‑1 (MQ=255)

                                                                                                                                        cacgCCCTTCCAAAAACCG‑AAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTAt     <  2:2619059/137‑1 (MQ=255)

                                                                                                                                        cAAGCCCTTCCAAAAAACG‑ATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTAt     <  1:4435976/140‑1 (MQ=255)

                                                                                                                                           gCCCTTCCCAAAAACT‑ATAAAAATAATCTGCTTATTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg  >  1:2798097/1‑140 (MQ=255)

                                                                                                                                           gCCCTTCCCAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATAAg  >  1:3702058/1‑140 (MQ=255)

                                                                                                                                           gCCCTTCCAAAAAACT‑ATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg  <  1:1632089/140‑1 (MQ=255)

                                                                                                                                           gCCCTTCAAAAAAACG‑ATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg  <  2:5065840/140‑1 (MQ=255)

                                                                                                                                           gCCCGTCCCAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg  <  2:608632/140‑1 (MQ=255)

CTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACG‑ATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAG  >  NZ_CP034943/3851115‑3851393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`