BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,274	A→C	18.4%	F34C (TTT→TGT)	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,274	0	A	C	18.4%	625.1 / 61.2	315	F34C (TTT→TGT)	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (177/69); new base C (2/66); total (180/135)
Fisher's exact test for biased strand distribution p-value = 4.74e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 320 total aligned reads displayed.

TGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGATAGCGCAGCGCAAAT  >  NZ_CP034943/3851136‑3851413

 ggAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAAc                                                                                                                                           >  1:1840564/1‑140 (MQ=255)

        tctTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTg                                                                                                                                    >  1:1753113/1‑140 (MQ=255)

            ttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGcttg                                                                                                                                >  1:3543613/1‑139 (MQ=255)

             tCGCTAGTTTTATCACCTTGTAATCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTAGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GGCCTTCACAAAAA‑CGATAAAAAGAATCTGcttct                                                                                                                               >  1:2733413/1‑140 (MQ=255)

                      ttATCACCTTGTACGAATAACCTTCAATTTACTCGTACTGGTTTTTTTTAATTGACCCTGTCGTTTTTACTTTTATCTTGTTTCTATCTTAGGCAC‑GCCCTTCCAAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCg                                                                                                                      <  2:2364807/140‑1 (MQ=255)

                        aTCACCTTCTAAGCAAAACATCCAAAGAACTGGGACAGTTCTACTAAAAATGAACCAGTCGTTATTACCTGTATCATGATTCGAACGTATGCAA‑GCCCTACCAAAAAA‑CTATAACACGACTCTGCTTCTTCAAGTTCGCa                                                                                                                    <  2:5080034/140‑1 (MQ=255)

                           attttttAAGAATAACGTTAAATTTACTCGCACAGTTATTCTATAAATTAACAAGTCTTTATTACTTTTATCTTTATTCGAGGTTAGGCAA‑GCCCTCCCCAAAAA‑CTAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGa                                                                                                                 <  2:2878732/134‑1 (MQ=255)

                           aCCTTGTAAGAATAACCTACAATGCCCGCGCACCGCTCTTAATTAAATCAACCCTTCTTTTTTCCCTGTAACATTATTCCCCCGTATCAAA‑CCCCGGCCAAAAAC‑CCTTAACACGACTCTGCTTCTTCAAGTTCGCAAGa                                                                                                                 <  2:4241890/140‑1 (MQ=255)

                             cTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACa                                                                                                               >  2:2421875/1‑140 (MQ=255)

                             cTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAAAGAATAGATATTAAGCAA‑GCCCGTACCAAAAA‑CGAAAAAAAGACACTTCTTCTTAAAGTTCTCAAcaca                                                                                                               >  1:4749686/1‑140 (MQ=255)

                                ttaataaTAACCTTAAAAGTACTCGTAACGGTCTTAAATAACTGAACCAGTCTTTTTTACCTTTAAAATTATTGGATCGTAGGCAC‑CCCGTTCCAAAAAA‑CGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATaa                                                                                                            <  2:2859476/134‑1 (MQ=255)

                                 tAAGCAAAACCTTAAAATTCTTCGTACAGTTCTTCTATAAATCAACCAGTGTTTATTACCTTTATCATTATTCGATCGTATCCAA‑GCCCTTCCAAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATaac                                                                                                           <  2:2476314/140‑1 (MQ=255)

                                      aTAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACtt                                                                                                      >  2:3779337/1‑140 (MQ=255)

                                       tAACCTTCAAAGTACTCGGACAGGTCTTCTATACATGAACCAGTCGGTATTACCTGTATCATGATTCGATCGTATGCCA‑GCCCTTCCCAAAAA‑CGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATACCAACTtg                                                                                                     >  2:1433535/1‑140 (MQ=255)

                                       tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                                     >  1:681303/1‑140 (MQ=255)

                                       tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                                     >  2:3950117/1‑140 (MQ=255)

                                       tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                                     >  1:1502773/1‑140 (MQ=255)

                                       tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGAACTAACAACAtg                                                                                                     >  1:997025/1‑140 (MQ=255)

                                       tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTATTCAAGTTCGCAAGACATAACAACTtg                                                                                                     >  1:1195567/1‑140 (MQ=255)

                                       tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGATTCTTCAAGTTCGCAACACATAACAACAtg                                                                                                     >  2:2665278/1‑140 (MQ=255)

                                        aaCCTACAAATTACTCGCACAGGTCTTATAAAAAGCAACCTGTTTTTATTCCCTTTATCATTATTCGAGCGTCTCCAC‑CCCATACCCAAAAC‑CCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtga                                                                                                    <  1:3427941/140‑1 (MQ=255)

                                          ccTTCAAAGTACTGGTACTGTTCTTATATAAATTAACCATTCGTTATTACCTGTATCATGATTCGATCGTTTCAAA‑GCCTTTCCAAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                                  <  2:529549/140‑1 (MQ=255)

                                          ccTTAAATGAACGCGCACAGGTCTTCTATAAATCAACCAGTGGTTATTACCTTTATCATTATTGGAGCGTATCAAA‑GCCCTACCCAAAAC‑CGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                                  <  2:3211520/140‑1 (MQ=255)

                                          ccTTAAAATTACACTCACAGGTTTTCTATACATGAACCAGTCGTTATTCCCTTTATCTTTTTTCTATCGTCTGCAC‑GCCCTCCCAAAAAC‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                                  <  2:1244310/140‑1 (MQ=255)

                                          ccTTAAAAGTACGGGGACTTTTCTTAAATAAATCAACGATTCTTTATTACCTGTATCTTTATTCGATCGTAGGCAA‑CCCCGCCCCAAAAA‑CGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                                  <  2:2158270/140‑1 (MQ=255)

                                          ccTGCAACGTCCTCGGAGAGGTCTTATATCAATGACCCAGTCTTTATTACGTTTAGCTTGATACGATCTTCTGCAC‑CCCCTGCCACAAAC‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                                  <  2:3074811/140‑1 (MQ=255)

                                          catcaaaaTTACTGGGACAGTTTTTATAAAAATCACCCTGTTTTTATTACCTTTATATTGATTCGATCGTAGCAAA‑GCCCTTCCAAAAAA‑CCAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                                  <  1:2914328/135‑1 (MQ=255)

                                          acTTAAAATTACTGGCACAGGATTTATATAAATGACCCAGTCTTTATTACATTTATCATTATTCGATCGTATGCAA‑GCCCTCCCCAAAAC‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                                  <  2:1098241/139‑1 (MQ=255)

                                               aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCt                                                                                             >  2:3876336/1‑140 (MQ=255)

                                                     cTCGGACCGGTCTTCTATAAATCAACCCTTCTTTATTACCTGTATCATTTTTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                                                       <  2:4533505/140‑1 (MQ=255)

                                                     cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAAAGACTCTGCTTCTTCAATTTCGCAAGACATAACAAAATTATGAGGATTAatag                                                                                       >  2:1053720/1‑140 (MQ=255)

                                                     cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCAA‑GCCCTTCCCAAAAA‑CGATAAAAAGACTCTGCTTCTTCCAGTTAGCAAGACATAACAACTTGATGAGGCTTaatat                                                                                       >  1:713055/1‑137 (MQ=255)

                                                     cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑TCCCTTCCCAAAAA‑AGATAAAACGACTATGCTTCTTCAAGTTAGCAAGACATAACAAATTGATGAGGCTTAagag                                                                                       >  1:799011/1‑140 (MQ=255)

                                                     cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTGCCAAAAA‑CGATAAAACGACTCTGCTTCTTGAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                                                       >  1:1126864/1‑140 (MQ=255)

                                                     cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                                                       >  2:4740187/1‑140 (MQ=255)

                                                         tagtgttcttttATAAATTACCCATTCTTTATTACTTGTATAATGTTTCTATGGTTGGCAC‑CCCCTCCCCAAAAC‑CCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                                                                   <  1:4185815/134‑1 (MQ=255)

                                                             ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATg                                                                               >  2:1568265/1‑140 (MQ=255)

                                                             ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACCACGCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATg                                                                               >  1:508369/1‑140 (MQ=255)

                                                             ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTTATGAGGCTTAAGAGCGATCATg                                                                               >  2:3329674/1‑140 (MQ=255)

                                                             ggTCTTCTATAAATGAACCAGTCGTTATTACCAGTAACATGAATAGATCGTATGCAA‑GCCCGTCCCAAAAA‑CGATAAAACCACTCAGCTTCATAAAGTTCGCCACAAATAACAACGTGATTAGGCTTAATATCGATAAtt                                                                               >  1:4133944/1‑139 (MQ=255)

                                                                       aaaTTAACCCGTCTTTATTACCTTTACCATTATGCCATCGTCTCAAA‑CCCCTACCAAAAAA‑CTAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGc                                                                     <  1:3169668/140‑1 (MQ=255)

                                                                        aaTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATTACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGcc                                                                    >  1:3888008/1‑140 (MQ=255)

                                                                        aaTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑AGATAAAACGACTCTTCTTCTTCAAGTTCGCAAGACATAACAACATGATGAGGCTTAAGAGCGATAATGTCTAAAtctgt                                                                    >  1:4360103/1‑136 (MQ=255)

                                                                        aaTGAACCAGTCGGTATTACCGGTATCATGGTTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGCAAAAACGACTATGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAATAGCGATCATGTCTAAAATGcc                                                                    >  1:2086773/1‑140 (MQ=255)

                                                                        aaTGAACCAGTCGATATTACCGGGATCAGGATTCGATCGTATGCAA‑GCCCGTCACAAAAA‑CGAAAAAACGACACTGCTTCTTCAAGGTCGCAAGACCTAACCACTTGATGAGGGTTAAGACCGGTCAGGTCTAAATTGcc                                                                    >  2:2352330/1‑140 (MQ=255)

                                                                         aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAAAAACTTTATGAGGCTTAATAGCGATCATTTCTAAATTTCCg                                                                   >  1:409900/1‑140 (MQ=255)

                                                                         aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCGTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAAATTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCg                                                                   >  1:5047012/1‑140 (MQ=255)

                                                                         aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑CCCCTTCCCAAAAA‑CGATAAAACGACTATGCTTCTTCAACTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGGCg                                                                   >  1:171284/1‑140 (MQ=255)

                                                                            acccAGTCTTTATTACCTGTAACATGATTCGATCGTTGGAAA‑GCCCTACCCAAAAA‑CGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                                                <  2:4574993/138‑1 (MQ=255)

                                                                            acccAGTCGTTTTTACCGTTAACATTTTTCGATCGTATCCAC‑CCCCTCCCAAAAAA‑CCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAGTTGCCGATa                                                                <  1:3324313/138‑1 (MQ=255)

                                                                            aaCCTGTCTTTATTACCTTTATAATGATTCGATCGTATCCAA‑GCCCTTCCCAAAAA‑CTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                                                <  2:199165/140‑1 (MQ=255)

                                                                            aaCCAGTGTTTATTACCTTTATAATGTTTCTTTCTTA‑GCAACGCCCAACAAAAAAA‑CGAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                                                <  1:2266305/140‑1 (MQ=255)

                                                                            aaCCAGTCTTTATTACGTGTATCTTGATTCGATGTTATGCAA‑GCCGTTCCCAAACA‑CTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                                                <  2:3251581/140‑1 (MQ=255)

                                                                             aCCTGTTGTTTTTACTTGTATAATTTTTCTATGGTATCAAC‑CCCCTTCAAAAAAA‑CTAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATAc                                                               <  1:945599/140‑1 (MQ=255)

                                                                             aCCAGTCGTTTTTACCTGTATCATGATTCGATCGTATTCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAATAGCGATCATGTGTAAATTGcccctac                                                               >  1:1961598/1‑135 (MQ=255)

                                                                             aCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTGCCCAAAA‑CGCTAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATAc                                                               >  1:1392274/1‑140 (MQ=255)

                                                                              ccAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                                                              >  2:357148/1‑140 (MQ=255)

                                                                                          aCCTGTATAATGATTCGATCGTATGCAA‑GCCATTCCCAAAAA‑AGATAAAACGACAATTCTTCTTCAAGTTCGCAAGACATAACAACTTGTTGAGGATTAAGATCGATCATGTCTAAATTGCCGATACTGCTTATATCGGt                                                  >  2:4751311/1‑140 (MQ=255)

                                                                                            cTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTa                                                >  2:490399/1‑140 (MQ=255)

                                                                                              ttataATGATTCGATCGTCGGCAA‑CCCCTCCCAAAAAA‑CGATACCACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAtt                                              <  1:2386858/139‑1 (MQ=255)

                                                                                              ttATCATTTTTCGTTCGTCGGCAC‑GCCCTCCCAAAAAA‑CCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAtt                                              <  1:220778/139‑1 (MQ=255)

                                                                                               tATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTTCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt                                             >  1:3981206/1‑140 (MQ=255)

                                                                                               tATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGATCGATCATGTCTAAATAGCCGATACTGCTTATAGCGGTTAttt                                             >  1:1311151/1‑140 (MQ=255)

                                                                                                aTCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTc                                            >  2:88011/1‑140 (MQ=255)

                                                                                                    ttaTTCGATCGTATGCAA‑GCCATGCCCAAACC‑CTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATa                                        <  1:5118583/138‑1 (MQ=255)

                                                                                                      aTTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                                      >  2:2810071/1‑140 (MQ=255)

                                                                                                         cttttttATGAAA‑GCCCTTCCAAAAAA‑CTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGCTATTTCCATAAAATc                                   <  1:1002356/134‑1 (MQ=255)

                                                                                                         cGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATAGTGCTTATAGCGGTTATTTCCATAAAATc                                   >  1:341539/1‑140 (MQ=255)

                                                                                                         cGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATc                                   >  1:2641762/1‑140 (MQ=255)

                                                                                                         cGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATc                                   >  2:178085/1‑140 (MQ=255)

                                                                                                           atgttttGCAT‑GCCCTGCAAAAAAA‑CTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCtt                                 <  2:697621/134‑1 (MQ=255)

                                                                                                           agattATGAAA‑GCCCTTCCAAAAAA‑CCATAACACGACTCTGCTTATTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCtt                                 <  2:1344806/136‑1 (MQ=255)

                                                                                                           aTCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCtt                                 >  1:1801626/1‑140 (MQ=255)

                                                                                                               tATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTcccc                             >  1:4531266/1‑140 (MQ=255)

                                                                                                               tATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTcccc                             >  2:4379375/1‑140 (MQ=255)

                                                                                                                 ttaaa‑GCTCTTCCCAATAT‑CTATAAAACGACTCTGCTTATTCAAGTTCGCAATACATAACAACTTGATGAGTCTTAAGATCGATCATTTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt                           <  2:1905815/137‑1 (MQ=255)

                                                                                                                 tGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt                           >  2:223468/1‑140 (MQ=255)

                                                                                                                 tGCAA‑GCCCTTCCCAAAAA‑CCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt                           <  2:3685791/140‑1 (MQ=255)

                                                                                                                  tgcacccccTTCCAAAAAA‑CGAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt                           <  2:1916004/134‑1 (MQ=255)

                                                                                                                   aaa‑GCCCTTCCAAAAAA‑AGAAACAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTAt                         <  1:2854523/139‑1 (MQ=255)

                                                                                                                    cacccccTCCCAAAAAA‑CGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTAt                         <  1:4441895/136‑1 (MQ=255)

                                                                                                                       gCCCTTCCCAACAC‑CCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg                      <  2:2733153/140‑1 (MQ=255)

                                                                                                                       gCCCTTCCAAAAAA‑CTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg                      <  1:1632089/140‑1 (MQ=255)

                                                                                                                       gCCCTCCCAAAAAC‑CCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg                      <  1:2042558/140‑1 (MQ=255)

                                                                                                                       gCCCGTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg                      <  2:608632/140‑1 (MQ=255)

                                                                                                                       ccccTTCCCAAAAA‑CGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg                      <  2:2577372/139‑1 (MQ=255)

                                                                                                                          cTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCACTTATCATTTg                   >  2:3001054/1‑140 (MQ=255)

                                                                                                                           ttCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTgg                  >  1:292815/1‑140 (MQ=255)

                                                                                                                           ttCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTgg                  >  2:1659360/1‑140 (MQ=255)

                                                                                                                            tCCCAACCA‑CCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGCTCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGa                 <  2:4133944/140‑1 (MQ=255)

                                                                                                                            tCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTTGa                 >  1:4968572/1‑140 (MQ=255)

                                                                                                                            tCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGa                 >  1:4823443/1‑140 (MQ=255)

                                                                                                                            tCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGGTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATCCTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGa                 >  2:4663352/1‑140 (MQ=255)

                                                                                                                            tCCCAAAAA‑CGATAAAACGACTCTGCTACTTCAAGTTCGCAAGAACAAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGGTGGa                 >  1:2116603/1‑140 (MQ=255)

                                                                                                                            ccccAAACA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGa                 <  2:1502686/139‑1 (MQ=255)

                                                                                                                             cccAAAAACCGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACGTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGa                 <  1:2813354/140‑1 (MQ=255)

                                                                                                                                 aaaa‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGATagcg            <  2:803609/140‑1 (MQ=255)

                                                                                                                                 aaaa‑CCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGATagcg            <  1:3074248/140‑1 (MQ=255)

                                                                                                                                    accGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGATagcgca          <  1:4568132/138‑1 (MQ=255)

                                                                                                                                       gATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGATagcgcagcg       <  1:88011/140‑1 (MQ=255)

TGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGATAGCGCAGCGCAAAT  >  NZ_CP034943/3851136‑3851413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`