BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,991,245	0	G	T	25.8%	286.3 / 38.2	182	intergenic (+46/‑161)	gbsR/bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand): ref base G (55/80); new base T (47/0); total (102/80)
Fisher's exact test for biased strand distribution p-value = 3.21e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 182 total aligned reads displayed.

AGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTT  >  NZ_CP034943/2991107‑2991378

  ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTaa                                                                                                                                     >  2:401343/1‑140 (MQ=255)

     tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGTAAACCGGGCGTTTTTGTGTTCTGTTTTATGTAATta                                                                                                                                  >  1:2839952/1‑140 (MQ=255)

     tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTTTGTAATta                                                                                                                                  >  2:2395255/1‑140 (MQ=255)

     tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATTTAATta                                                                                                                                  >  2:2622694/1‑140 (MQ=255)

     tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTGTGTTTTTTTTTTTTTTTGttaata                                                                                                                                  >  1:666087/1‑135 (MQ=255)

            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTGTTATTACT‑ACaaa                                                                                                                          >  1:2807815/1‑140 (MQ=255)

            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTGTGTAATTACT‑ACaaa                                                                                                                          >  2:2423170/1‑140 (MQ=255)

            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTATTTTTTATGTAAGTACT‑ACaaa                                                                                                                          >  2:22361/1‑140 (MQ=255)

            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTGTTTTGTTTTTTTTTTTTTTATTAAT‑ACaaa                                                                                                                          >  1:1082739/1‑140 (MQ=255)

             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTTTTTTATTTCT‑AAAAAt                                                                                                                         >  1:2057425/1‑140 (MQ=255)

             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATTTAATTACT‑ACAAAt                                                                                                                         >  2:687688/1‑140 (MQ=255)

             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTTTGTAATTAAT‑ACAAAt                                                                                                                         >  2:1666522/1‑140 (MQ=255)

               tATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATTTAATTACT‑ACAAAttt                                                                                                                       >  2:89401/1‑140 (MQ=255)

                   aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATTTAATTACT‑ACAAAATTTaaa                                                                                                                   >  2:507447/1‑140 (MQ=255)

                    ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTTTTTTATTACT‑AAAAAatttttaa                                                                                                                  >  1:584100/1‑135 (MQ=255)

                      ggCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTAAGTAATTAAT‑ACAAAATTGaaaaaa                                                                                                                >  2:3539325/1‑139 (MQ=255)

                       gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAACCCGGGCGTTTTTTTGTTCTTGTTTTTGTAATTAGT‑ACAAATTTTAAAAATa                                                                                                               >  2:2912317/1‑140 (MQ=255)

                       gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATTTAATTACT‑ACAAAATTGAAAAATa                                                                                                               >  2:2079230/1‑140 (MQ=255)

                       gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTTTTTTATTAAT‑ACAAAATTTAAAAATa                                                                                                               >  1:296764/1‑140 (MQ=255)

                           acactgCTTTCTGAACCGGTGCCTCCCGTGGAAATTTATAAAATAATACCGATTCAAACAATCTAAAAGAAAA‑A‑CGCCTGGGAAACCGGGCTTTTTTTTTTTCTTTTTTATGTTATTACT‑ACAAATTTGAAAAATACggg                                                                                                           <  1:2092447/135‑1 (MQ=255)

                            aaCGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTGTGTTTGTTGTTTTTTATGTAATTAAT‑ACACAATTTAAAAATaatgga                                                                                                          >  2:1437913/1‑135 (MQ=255)

                              cGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGaaa                                                                                                        <  2:644269/140‑1 (MQ=255)

                                ggttGCTGAATCGGTGCAGAGCGGGTAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGaaaaa                                                                                                      <  2:1468161/138‑1 (MQ=255)

                                     cTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATcc                                                                                                 <  2:3827314/140‑1 (MQ=255)

                                     cTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTGTTTGTTCTTTTTTATTTAAAAAAT‑AAAAATTTTAAAAAAACGGGTAAAACAtac                                                                                                 >  2:1244010/1‑138 (MQ=255)

                                     cTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAAATACT‑ACAAATTTGAAAAATAAGGGAAAAACATcc                                                                                                 >  2:707702/1‑140 (MQ=255)

                                          tCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATTTAATTAAT‑ACAAAATTTAAAAAAAATGGAAAAACATACCAatt                                                                                            >  2:37498/1‑140 (MQ=255)

                                           cGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTTTAATTAAT‑AAAAAAATTTAAAATAAGGGGAAAAAATACCAATTa                                                                                           >  1:1588152/1‑140 (MQ=255)

                                              tGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTTTTATTAATAAAAAATTTAAAAAAAAAGGGAAAAAAAACCAAttaatt                                                                                         >  1:3500336/1‑136 (MQ=255)

                                                 ctcgCGGGGAAATTTTTAAATTTATACCGATTCAAACAAAGTAAAACAAAC‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCa                                                                                     <  1:1888493/137‑1 (MQ=255)

                                                 atcgggggaAAATTTTTAAATTTATACCTATTCAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCa                                                                                     <  1:2977573/135‑1 (MQ=255)

                                                 agagCGGGTAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAC‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCa                                                                                     <  1:3672434/140‑1 (MQ=255)

                                                  gagCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAAATTCT‑AAAAATTTTAAAAAAAAGGGAAAAACATGACATTTAATTCCaa                                                                                    >  2:764807/1‑140 (MQ=255)

                                                   agCGGGGAAATTTTTAAATTTATACCGATTGAAACACAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTGTTTGTGTTATTGTTTCTTGAATTAAT‑ACAAATTTGAAAAATAAGGGACAAACATACCCTTTAATTcccca                                                                                   >  2:235778/1‑137 (MQ=255)

                                                     cGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTTTAATTAAA‑ACAAAATTTAAAAATAATGGAAAAAAATACCAATTAATTCCaaaaa                                                                                 >  2:1521581/1‑139 (MQ=255)

                                                     cGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTGGTTTTGTTCTTTTTTTTGTAATTACT‑AAAAATTTGTAAATTACGGGAAAAAAATGCCAGTTAATTCCAAAat                                                                                 >  2:2755007/1‑140 (MQ=255)

                                                       gggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATTTAATTAAT‑ACAAAATTTTAAAATAAGGGAAAAAAATCCCATTTTATTACAAAaaat                                                                               >  2:3416310/1‑140 (MQ=255)

                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAT‑A‑CGCCTGGGAAACCGGGCGTGTGTTTGTTGTTTTTTATGTAATTACT‑ACCATTTTGAAAAATAAGGGTAAAAGATACCCTTTAGATCCAACAAAtt                                                                              >  2:2049267/1‑140 (MQ=255)

                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTTTTTAATTAAT‑ACAAAATTGAAAAAAACGGGAAAAAAAAACCAATTACTTCCAAAAAAtt                                                                              >  2:455914/1‑140 (MQ=255)

                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATGTAATTACT‑ACAAAATTTTAAAATACGGGGAAAAAATGCCATTTAATTGCAAAATAtt                                                                              >  2:2553152/1‑140 (MQ=255)

                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTAATTAAATAAT‑AAAAAATTTAAAAATACCGGAAAAAAAAACCAATTAAATCAAAAATAtt                                                                              >  2:1344168/1‑140 (MQ=255)

                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTGTAAATTCT‑ACAAAATTTGAAAATAAGGGAAAAAAATCCCATTTAATTGCAAAATAtt                                                                              >  2:201500/1‑140 (MQ=255)

                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTGTTGTTGTTTTTTATGTAATTACT‑ACAAAATTGAAAAATACGGGAAAAACATACCCTTTACTTACAAAATAtt                                                                              >  2:3758514/1‑140 (MQ=255)

                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTGTTTTTGTTGGTGTTTATTTAATTAAT‑ACAAAATTGAAAAATAAGGGAAAAACATACCAGTTAATTCCAAAATAtt                                                                              >  2:297170/1‑140 (MQ=255)

                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTGTGTTTGTTGTTTTTTATGTAATTTAT‑AAAAATTTTAAAAATAAGGGAAAAAAATGGCAATTACATACaaaaatgt                                                                              >  2:2512050/1‑136 (MQ=255)

                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGGTGTTTTTTTCTTTTTTTTTTAATTAAT‑ACTAATTTGTAAAATACGGGAAAAAAATACCATTTACTTCCAAAATAtt                                                                              >  2:1940272/1‑140 (MQ=255)

                                                             tttttAAATTTCTACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTGTTTTTGTTCTTTTTTTTGTAAATAGT‑CCAAAATGGTAAAAAACGGGCAAAACCTCCGAGTGAGTTGCAAAATATTTGATg                                                                         >  2:1101174/1‑140 (MQ=255)

                                                              ttttAAATTTATACCTTTTCAAACAAAGTAAAAAAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                        <  1:1964433/140‑1 (MQ=255)

                                                              ttttAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                        <  2:2710111/140‑1 (MQ=255)

                                                                       tataCCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAATTACT‑AAAAATTTGAAAAATACGGGAAAAAAATACCAATTTCTTCCAAAATATTTGATGGTATtttttt                                                               >  2:2125713/1‑138 (MQ=255)

                                                                        ataCCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                                              <  1:1842680/140‑1 (MQ=255)

                                                                        ataCCGAAAGAAACAAAGTAACCCAAAC‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTACTTTTTTATGTAATTACT‑CCACATTTGAAAAATACGGGACAAACATCCCATGTACTTCCAAAATATTTGATGGTAATTTTATg                                                              <  1:3661528/140‑1 (MQ=255)

                                                                        ataCAGATTGAAACAACGAAAAACAAAA‑A‑CGCCTGGGAAACCGGTCGTTTTTTTGTTCTTTTTTATGTAATTACA‑ACAAATTTGAAAAATACGGGAAAAACAGCCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                                              <  2:839077/140‑1 (MQ=255)

                                                                             taTTTAAACAAATAAAAACAAAC‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                         <  1:2395255/139‑1 (MQ=255)

                                                                             gATTCAAAAAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                         <  1:2622694/140‑1 (MQ=255)

                                                                              aTTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAATTACT‑ACAAATTTTAAAAATACGGGAAAAAAATACCATTTTATTACAAAATATTTTATGGTAATTTTATGTTGGTa                                                        >  2:872860/1‑140 (MQ=255)

                                                                              aTTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGCCGTTTTTTTGTTATTTTTTAAGTAAATACA‑ACAAAAATGAAAAATACAGGAAAAACATCACAGGTACTGACCAAATAGTTTATGGTAATTTTATGTAGGtt                                                        >  2:1710917/1‑139 (MQ=255)

                                                                                  aaaaaaAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                    <  1:171005/140‑1 (MQ=255)

                                                                                  aaaCAAATTAAAACAAAC‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                    <  1:1114530/140‑1 (MQ=255)

                                                                                  aaaCAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                    <  2:1776709/140‑1 (MQ=255)

                                                                                  aaaCAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                    <  2:2181988/140‑1 (MQ=255)

                                                                                        aGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATTTAATTAAT‑ACAAATTTGAAAAAAAAGGGAAAAACATCCCATTTAATTCCAAAATATTTGTTGGTTATTTTATGTTGGTAAAATTTTTAt                                              >  2:2295065/1‑140 (MQ=255)

                                                                                        aGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTGTTGTTGGTTTTTTAGTAATTACT‑AAAAATATGAAAAAAAAGGGAAAAAAATACCAATAAATTCCAAAATATTTTTTGTTAATATTATTGATGTAAAAATTGtct                                              >  2:735624/1‑138 (MQ=255)

                                                                                            caacaaac‑a‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                          <  1:2179022/139‑1 (MQ=255)

                                                                                            acaCAAAC‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                          <  1:9498/138‑1 (MQ=255)

                                                                                            aaacaaac‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACATATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTGGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                          <  2:3597532/140‑1 (MQ=255)

                                                                                            aaacaaac‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                          <  1:1882058/140‑1 (MQ=255)

                                                                                            aaaaaaAC‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                          <  1:2422797/140‑1 (MQ=255)

                                                                                            aaaCTAAA‑A‑CGCCTGTGATACCGGGCGTTTTTTTGTTCTTTTTTATGTTATTACT‑ACAAATTTGAAAAATACGGGAAAAAGATCCCATTTACTTCCAATTTATTTGATGGTAATTTTATGTAGGTATAATTTGTATTTgg                                          <  2:984331/140‑1 (MQ=255)

                                                                                            aaaCAACA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                          <  1:455914/140‑1 (MQ=255)

                                                                                            aaaCAAAA‑A‑CGCCTGGGAAACCTGGCGATTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGCATAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                          <  2:350903/140‑1 (MQ=255)

                                                                                            aaaCAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                          <  2:1161980/140‑1 (MQ=255)

                                                                                            aaaCAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                          <  1:1666522/140‑1 (MQ=255)

                                                                                            aaaCAAAA‑A‑CGCCTGGGAAACCGGGCGATTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                          <  2:2934005/140‑1 (MQ=255)

                                                                                            aaaCAAAA‑A‑CGCCTGGGAAACCGGGAGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACTGGAAAAACATACCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                          <  2:3029425/140‑1 (MQ=255)

                                                                                             aaCAAAC‑C‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGc                                         <  1:2587990/140‑1 (MQ=255)

                                                                                             aaCAAAACA‑CGCCTGGGAAACCGGGCGTTATTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                          <  1:159144/140‑1 (MQ=255)

                                                                                             aaCAAAA‑ACCGCCTGGGACACCGGGCGTTTTATTGTTCTTTTTTATGTAAATACT‑ACAAATTTGAAAAATACGGGAAAACCATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                          <  1:2866537/140‑1 (MQ=255)

                                                                                             aaCAAAA‑A‑CGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTATTTTATTACA‑AAAAATTTTTAAAATAAGGGCAAAAAATACCATTTAATTGCAAAAAATTTGTTTGTAATTTTATTTGTGTAAAATTTTTAttttgg                                         >  2:336633/1‑137 (MQ=255)

                                                                                               cAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCg                                       <  1:812805/140‑1 (MQ=255)

                                                                                               aaaac‑a‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCg                                       <  1:507495/139‑1 (MQ=255)

                                                                                                  aa‑a‑CGCCTGGGAAACCGGGCGTTTTTTTGTTTTTTTTTTTTTAATTAAT‑ACAAATTTTAAAAATAAGGGAAAAAAATACCATTTACTTGAAAAAAAATTGATGGTTATTTTATGTTGGTAAAATTTTTATTTGGGCggtg                                    >  2:2926412/1‑137 (MQ=255)

                                                                                                  aa‑a‑CGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTGTTATTAAT‑ACAAAATTTTAAAAAAAGGGCAAAACATCCCATTTTAATACAAAATATTTGGTGGTTATTTTATTTAGGTAAAATTTGTATTTGTGCGttg                                    >  2:1038113/1‑139 (MQ=255)

                                                                                                        gCCTGGGAATCCGGGCTTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                <  1:2211427/140‑1 (MQ=255)

                                                                                                        gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                <  1:37498/140‑1 (MQ=255)

                                                                                                        gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                <  1:1732861/140‑1 (MQ=255)

                                                                                                        gCCTGGGAAACCGGGAGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                <  1:2512050/140‑1 (MQ=255)

                                                                                                        cccTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                <  1:2381951/139‑1 (MQ=255)

                                                                                                            gggAAACCGGGCGTTTTTTTGTTCTTTTTTATGTACTTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGaggagg                            <  2:2904729/140‑1 (MQ=255)

                                                                                                             ggAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGt                           <  1:1437913/140‑1 (MQ=255)

                                                                                                                  ccGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaaa                      <  1:3230537/140‑1 (MQ=255)

                                                                                                                       cGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                 <  2:3717923/140‑1 (MQ=255)

                                                                                                                       cGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                 <  1:1374945/140‑1 (MQ=255)

                                                                                                                       cGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                 <  2:3181073/140‑1 (MQ=255)

                                                                                                                       cGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                 <  2:2167924/140‑1 (MQ=255)

                                                                                                                            ttttGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGc            <  1:3416310/140‑1 (MQ=255)

                                                                                                                                  tCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAat      <  1:1032759/140‑1 (MQ=255)

                                                                                                                                      ttttATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATAttt  >  2:563782/1‑140 (MQ=255)

                                                                                                                                      ttttATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATAttt  >  1:572696/1‑140 (MQ=255)

                                                                                                                                      ttttATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCGGAATAttt  >  1:3173321/1‑140 (MQ=255)

AGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑A‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTT  >  NZ_CP034943/2991107‑2991378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`