BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,991,252	0	C	A	27.5%	297.6 / 18.3	174	intergenic (+53/‑154)	gbsR/bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand): ref base C (45/79); new base A (47/0); total (95/79)
Fisher's exact test for biased strand distribution p-value = 1.60e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 175 total aligned reads displayed.

AGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTT  >  NZ_CP034943/2991115‑2991388

    tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTGTTATTACTAC‑aaa                                                                                                                                     >  1:2807815/1‑140 (MQ=255)

    tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTGTGTAATTACTAC‑aaa                                                                                                                                     >  2:2423170/1‑140 (MQ=255)

    tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTATTTTTTATGTAATTAATAC‑aaa                                                                                                                                     >  2:428038/1‑140 (MQ=255)

    tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTGTTTTGTTTTTTTTTTTTTTATTAATAC‑aaa                                                                                                                                     >  1:1082739/1‑140 (MQ=255)

    tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTGTTTTTGTTGATTTTTAATTAAATACAAC‑aaa                                                                                                                                     >  2:2977573/1‑140 (MQ=255)

    tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTGTTTGTTTTTTTTTTTTTGTAAATAATAC‑aaa                                                                                                                                     >  1:2807854/1‑140 (MQ=255)

     aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTTTTTTATTTCTAA‑AAAt                                                                                                                                    >  1:2057425/1‑140 (MQ=255)

     aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATTTAATTACTAC‑AAAt                                                                                                                                    >  2:687688/1‑140 (MQ=255)

           aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATTTAAATACTAC‑AAAATTTaaa                                                                                                                              >  2:507495/1‑140 (MQ=255)

           aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATTTAATTACTAC‑AAAATTTaaa                                                                                                                              >  2:507447/1‑140 (MQ=255)

            ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTTTTTTATTACTAA‑AAAatttttaa                                                                                                                             >  1:584100/1‑135 (MQ=255)

            ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTATTTAATTAATAA‑AAAATTGaaaa                                                                                                                             >  1:3202756/1‑140 (MQ=255)

            ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTTTTTAAATAAAAC‑AAAtttttaaa                                                                                                                             >  1:1437963/1‑135 (MQ=255)

            ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTGTTTTTGTTTGTTTTTTTTTAATTAATAA‑AAAATTGtaaa                                                                                                                             >  1:1395834/1‑140 (MQ=255)

              ggCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTAAGTAATTAATAC‑AAAATTGaaaaaa                                                                                                                           >  2:3539325/1‑139 (MQ=255)

               gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATTTAATTACTAC‑AAAATTGAAAAATa                                                                                                                          >  2:2079230/1‑140 (MQ=255)

                   acactgCTTTCTGAACCGGTGCCTCCCGTGGAAATTTATAAAATAATACCGATTCAAACAATCTAAAAGAAAAA‑CGCCTGGGAAACCGGGCTTTTTTTTTTTCTTTTTTATGTTATTACTAC‑AAATTTGAAAAATACggg                                                                                                                      <  1:2092447/135‑1 (MQ=255)

                    aaCGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTGTGTTTGTTGTTTTTTATGTAATTAATAC‑ACAATTTAAAAATaatgga                                                                                                                     >  2:1437913/1‑135 (MQ=255)

                      cGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGaaa                                                                                                                   <  2:644269/140‑1 (MQ=255)

                         cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTAATAA‑AAATTTGAAAAAAAAGGGaaaaaa                                                                                                                >  2:3520109/1‑139 (MQ=255)

                         cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTTTGTAATTAAGAA‑AAATTTTAAAAAAAAGGGgaaaaa                                                                                                                >  1:1705449/1‑139 (MQ=255)

                         cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTGTGTTTGTTGTTTTTTATGTAATTAATAC‑AAATTTTTAAAATAATGGacaaaa                                                                                                                >  1:3475984/1‑139 (MQ=255)

                            gCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATc                                                                                                            <  2:714908/140‑1 (MQ=255)

                             cTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATcc                                                                                                           <  2:3827314/140‑1 (MQ=255)

                             cTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTGTTTGTTCTTTTTTATTTAAAAAATAA‑AAATTTTAAAAAAACGGGTAAAAC‑Atac                                                                                                           >  2:1244010/1‑138 (MQ=255)

                                   cGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTTTAATTAATAA‑AAAAATTTAAAATAAGGGGAAAAA‑ATACCAATTa                                                                                                     >  1:1588152/1‑140 (MQ=255)

                                     gTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTGTGTTTGTTGTTTTTTTTTTTATTAAAAC‑AAAATTTAAAAAAAAGGGAAAAAA‑ATACCaagtaat                                                                                                   >  1:1645396/1‑134 (MQ=255)

                                         atcgggggaAAATTTTTAAATTTATACCTATTCAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCa                                                                                               <  1:2977573/135‑1 (MQ=255)

                                          gagCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAAATTCTAA‑AAATTTTAAAAAAAAGGGAAAAAC‑ATGACATTTAATTCCaa                                                                                              >  2:764807/1‑140 (MQ=255)

                                           agCGGGGAAATTTTTAAATTTATACCGATTGAAACACAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTGTTTGTGTTATTGTTTCTTGAATTAATAC‑AAATTTGAAAAATAAGGGACAAAC‑ATACCCTTTAATTcccca                                                                                             >  2:235778/1‑137 (MQ=255)

                                             cGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTTTAATTAAAAC‑AAAATTTAAAAATAATGGAAAAAA‑ATACCAATTAATTCCaaaaa                                                                                           >  2:1521581/1‑139 (MQ=255)

                                                ggAAATTTTTAAATTTATACCGCTTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTGGTTTTTTTGTTTTTTTTGTAAATAGTAC‑AAAATTGAAAAAAACGGGAAAAAA‑ATGCGATTTACTTACAAAATAtt                                                                                        >  2:2834523/1‑140 (MQ=255)

                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTAATTAAATAATAA‑AAAATTTAAAAATACCGGAAAAAA‑AAACCAATTAAATCAAAAATAtt                                                                                        >  2:1344168/1‑140 (MQ=255)

                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTTTTATTTTTTATGTAATTACTAC‑AAATTTTAAAAATACCGGGAAAAC‑AACCCATTTTCATACAACATAtt                                                                                        >  2:886894/1‑140 (MQ=255)

                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTGTAAATTCTAC‑AAAATTTGAAAATAAGGGAAAAAA‑ATCCCATTTAATTGCAAAATAtt                                                                                        >  2:201500/1‑140 (MQ=255)

                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTGTTGTTGTTTTTTATGTAATTACTAC‑AAAATTGAAAAATACGGGAAAAAC‑ATACCCTTTACTTACAAAATAtt                                                                                        >  2:3758514/1‑140 (MQ=255)

                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAAGGGAAAAAC‑ATGCCATTTACGTCCAAAATAtt                                                                                        >  2:1722912/1‑140 (MQ=255)

                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTGTGTTTGTTGTTTTTTATGTAATTTATAA‑AAATTTTAAAAATAAGGGAAAAAA‑ATGGCAATTACATACaaaaatgt                                                                                        >  2:2512050/1‑136 (MQ=255)

                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGGTGTTTTTTTCTTTTTTTTTTAATTAATAC‑TAATTTGTAAAATACGGGAAAAAA‑ATACCATTTACTTCCAAAATAtt                                                                                        >  2:1940272/1‑140 (MQ=255)

                                                     tttttAAATTTCTACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTGTTTTTGTTCTTTTTTTTGTAAATAGTCC‑AAAATGGTAAAAAACGGGCAAAAC‑CTCCGAGTGAGTTGCAAAATATTTGATg                                                                                   >  2:1101174/1‑140 (MQ=255)

                                                      ttttAAATTTATACCTTTTCAAACAAAGTAAAAAAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATgg                                                                                  <  1:1964433/140‑1 (MQ=255)

                                                      ttttAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATgg                                                                                  <  2:2710111/140‑1 (MQ=255)

                                                      ttttAAATTTAAACCGATTCAAACAAACTAAAACAAACA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATgg                                                                                  <  1:3565536/140‑1 (MQ=255)

                                                      ttttAAATTAATACCTATTCAAAAAAAGTAAAACAAACA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATgg                                                                                  <  1:2553152/140‑1 (MQ=255)

                                                       tttAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTATTTTATTAAAACAAAATTTAAAAAAAAGGGGAAAAAA‑AAACCA‑TTAAGTCCAAAAAATTTgttttt                                                                                 >  1:1936309/1‑135 (MQ=255)

                                                               tataCCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAATTACTAA‑AAATTTGAAAAATACGGGAAAAAA‑ATACCAATTTCTTCCAAAATATTTGATGGTATtttttt                                                                         >  2:2125713/1‑138 (MQ=255)

                                                                ataCCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                                                        <  1:1842680/140‑1 (MQ=255)

                                                                ataCCGAAAGAAACAAAGTAACCCAAACA‑CGCCTGGGAAACCGGGCGTTTTTTTGTACTTTTTTATGTAATTACTCC‑ACATTTGAAAAATACGGGACAAAC‑ATCCCATGTACTTCCAAAATATTTGATGGTAATTTTATg                                                                        <  1:3661528/140‑1 (MQ=255)

                                                                ataCAGATTGAAACAACGAAAAACAAAAA‑CGCCTGGGAAACCGGTCGTTTTTTTGTTCTTTTTTATGTAATTACAAC‑AAATTTGAAAAATACGGGAAAAAC‑AGCCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                                                        <  2:839077/140‑1 (MQ=255)

                                                                     taTTTAAACAAATAAAAACAAACA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                                   <  1:2395255/139‑1 (MQ=255)

                                                                     gATTCAAAAAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                                   <  1:2622694/140‑1 (MQ=255)

                                                                      aTTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAAATACTAC‑AAAATTTAAAAAAAAGGGAAAAAC‑ATACCAATTAATTACAAAATATTTGATGGTAATTTTTTGTAggga                                                                  >  2:568843/1‑138 (MQ=255)

                                                                      aTTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTGTTTTGTTGTTTTTTATGTAATTAATAA‑AAAATTTAAAAAAAAGGGAAAAAA‑ATACCATTTACATACAAAATAATTTATTGTAATTTTATTTAGGTa                                                                  >  2:841467/1‑140 (MQ=255)

                                                                      aTTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTTTTATTTATAA‑AAAAATTTAAAAAAAGGGAAAAACAAACCCATTTA‑GTAAAAAAAATTTTTTTGTAATtttatttatgga                                                                  >  1:3717923/1‑136 (MQ=255)

                                                                        tGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTGTTTTTGTTCTTTTTTATTTAAATACTAC‑AAATTTGAAAAATACGGGCAAAAA‑ATAACAGTTAATTACATAAAATTTTATTGTTATTTTATGTAGTTaaa                                                                >  2:2232668/1‑140 (MQ=255)

                                                                          aaaaaaAGTAAACAAAACA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                              <  1:745286/140‑1 (MQ=255)

                                                                          aaaCAAATTAAAACAAACA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                              <  1:1114530/140‑1 (MQ=255)

                                                                          aaaCAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                              <  2:1776709/140‑1 (MQ=255)

                                                                          aaaCAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                              <  2:2181988/140‑1 (MQ=255)

                                                                                aGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATTTAATTAATAC‑AAATTTGAAAAAAAAGGGAAAAAC‑ATCCCATTTAATTCCAAAATATTTGTTGGTTATTTTATGTTGGTAAAATTTTTAt                                                        >  2:2295065/1‑140 (MQ=255)

                                                                                aGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATTTAAATACTAA‑AAAATTTAAAAATACGGGAAAAAC‑ATACCATTTACTTACAAAAAATTTTTTTGTAAATTTATTTAGGTAAAATTTTTAt                                                        >  2:2909042/1‑140 (MQ=255)

                                                                                aGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTGTTTTTGGTGTTTTTTATGTAAATACTAA‑AAATTTTAAAAATAAGGGCAAAAC‑AACCGCTTTAATTGCAAAATTTTTGATGGTAATTTTATGTTGGTAAAATTTTTAt                                                        >  2:3057683/1‑140 (MQ=255)

                                                                                    caacaaaca‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                    <  1:2179022/139‑1 (MQ=255)

                                                                                    acaCAAACA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                    <  1:9498/138‑1 (MQ=255)

                                                                                    aaacaaacA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑ATATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTGGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                    <  2:3597532/140‑1 (MQ=255)

                                                                                    aaacaaacA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                    <  1:1882058/140‑1 (MQ=255)

                                                                                    aaacaaacA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                    <  2:256236/140‑1 (MQ=255)

                                                                                    aaacaaacA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCAATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                    <  2:296764/140‑1 (MQ=255)

                                                                                    aaaaaaACA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                    <  1:2422797/140‑1 (MQ=255)

                                                                                    aaaCTAAAA‑CGCCTGTGATACCGGGCGTTTTTTTGTTCTTTTTTATGTTATTACTAC‑AAATTTGAAAAATACGGGAAAAAG‑ATCCCATTTACTTCCAATTTATTTGATGGTAATTTTATGTAGGTATAATTTGTATTTgg                                                    <  2:984331/140‑1 (MQ=255)

                                                                                    aaaCAACAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                    <  1:455914/140‑1 (MQ=255)

                                                                                    aaaCAAAAA‑CGCCTGGGAAACCTGGCGATTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGCATAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                    <  2:350903/140‑1 (MQ=255)

                                                                                    aaaCAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                    <  2:2933856/140‑1 (MQ=255)

                                                                                    aaaCAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                    <  2:2057425/140‑1 (MQ=255)

                                                                                    aaaCAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                    <  2:1161980/140‑1 (MQ=255)

                                                                                     aaCAAAAACCGCCTGGGACACCGGGCGTTTTATTGTTCTTTTTTATGTAAATACTAC‑AAATTTGAAAAATACGGGAAAACC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                    <  1:2866537/140‑1 (MQ=255)

                                                                                     aaCAAAAA‑CGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTATTTTATTACAAA‑AAATTTTAAAAAAAAGGGAAAAAA‑AAGCCAATTAATTGAACAATATGTGATTGTAAATTTTTGTAGGTAAAATTTGTATTTTGc                                                   >  2:2413559/1‑140 (MQ=255)

                                                                                       cAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCg                                                 <  1:507447/140‑1 (MQ=255)

                                                                                       cAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCg                                                 <  1:812805/140‑1 (MQ=255)

                                                                                         aaaa‑CGCCTGGGAAACCGGGCGTTTTTTTGTTATTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGtt                                               <  2:1681856/140‑1 (MQ=255)

                                                                                          aaa‑CGCCTGGGAAACCGGGCGTTTTTTTGTTTTTTTTTTTTTAATTAATAC‑AAATTTTAAAAATAAGGGAAAAAA‑ATACCATTTACTTGAAAAAAAATTGATGGTTATTTTATGTTGGTAAAATTTTTATTTGGGCggtg                                              >  2:2926412/1‑137 (MQ=255)

                                                                                          aaa‑CGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTATGTAATTAATAC‑AAATTTGAAAAATACGGGACAAAA‑ATACCAATTAATTACAAAATATTTTATTGTAATTTTATGTAGGTAAAATTTTTATTTgggccgtc                                              >  2:3384659/1‑134 (MQ=255)

                                                                                          aaa‑CGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTGTTATTAATAC‑AAAATTTTAAAAAAAGGGCAAAAC‑ATCCCATTTTAATACAAAATATTTGGTGGTTATTTTATTTAGGTAAAATTTGTATTTGTGCGttg                                              >  2:1038113/1‑139 (MQ=255)

                                                                                          aaa‑CGCCTGGGAAACCGGGCGTGGTTTTGTTGTTTTTTATTTAATTACGTC‑AAAATTGTAAAATAAGGGCAAAAA‑ATCCCAGGTGAATCGCAAAAATTTGATGGTAATTGTATGTATGTAAAAATTGGATTTTGGCGttg                                              >  2:375153/1‑139 (MQ=255)

                                                                                               gCCTGGGAATCCGGGCTTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                          <  1:2211427/140‑1 (MQ=255)

                                                                                               gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                          <  2:1437963/140‑1 (MQ=255)

                                                                                               gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                          <  1:1732861/140‑1 (MQ=255)

                                                                                               cccTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                          <  1:2381951/139‑1 (MQ=255)

                                                                                                         ccccgCGGTTTTTTGTACTTTTTTCTGTAATTACTAC‑AAATGTGAACAATACGGGAAACAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaaa                                <  1:2423170/136‑1 (MQ=255)

                                                                                                         ccGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaaa                                <  1:3230555/140‑1 (MQ=255)

                                                                                                         ccGGGCGTGTTTTTGTTGTTTTTTATTTAAATAATAC‑AAATTTGAAAAATACGGGAAAAAA‑ATCCCATTTTCTTCCAAAATATTTTTTGGTAATTTTATTTAGGTAAAATTTTTATTTTGGCGTTAAGAGGAGGTGtaaa                                >  2:2754198/1‑140 (MQ=255)

                                                                                                              cGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                           <  2:3717923/140‑1 (MQ=255)

                                                                                                              cGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                           <  2:3181073/140‑1 (MQ=255)

                                                                                                              cGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                           <  2:707404/140‑1 (MQ=255)

                                                                                                              cGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                           <  2:2167924/140‑1 (MQ=255)

                                                                                                                         tCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAat                <  1:1032759/140‑1 (MQ=255)

                                                                                                                         tCTTTTTTATGTAATTACCAC‑AAATTTGAAAAATACGGGACACAC‑ATCCCATTTACTTCCAAAAGATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGACGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAat                <  1:1722912/140‑1 (MQ=255)

                                                                                                                             ttttATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATAttt            >  1:572006/1‑140 (MQ=255)

                                                                                                                             ttttATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCGGAATAttt            >  1:3173321/1‑140 (MQ=255)

                                                                                                                                       ttACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACttt  >  1:3762208/1‑140 (MQ=255)

AGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAA‑CGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATACGGGAAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTT  >  NZ_CP034943/2991115‑2991388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`