BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,991,245	G→T	29.7%	intergenic (+46/‑161)	gbsR → / → bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,991,245	0	G	T	29.7%	141.1 / 33.6	115	intergenic (+46/‑161)	gbsR/bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand): ref base G (27/51); new base T (34/3); total (61/54)
Fisher's exact test for biased strand distribution p-value = 2.33e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

CTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTA  >  NZ_CP034943/2991109‑2991381

ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACTCCTGGGACACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTa‑a                                                                                                                                             >  2:2043448/1‑140 (MQ=255)

ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGTGAAACCGGGCGT‑GTTTTTGTT‑CTTTTTTATGTac                                                                                                                                              >  2:3145525/1‑139 (MQ=255)

          tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTTTT‑GTTTTTTTTTTA‑A‑AT‑AAT‑Aa‑aaa                                                                                                                               >  1:516705/1‑140 (MQ=255)

          tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTTTT‑CTTTTTTATGTA‑A‑TT‑AAT‑AC‑aaa                                                                                                                               >  2:1043605/1‑140 (MQ=255)

          tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑GTTTTTTATTTA‑A‑TA‑ACT‑AC‑aaa                                                                                                                               >  2:754100/1‑140 (MQ=255)

          tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑AAT‑Aa‑aaa                                                                                                                               >  2:3178608/1‑140 (MQ=255)

          tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑GTTTTTTTTTTT‑A‑TT‑AAT‑Aa‑aaa                                                                                                                               >  1:1699397/1‑140 (MQ=255)

          tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑GTTTTTTATTTA‑A‑TT‑AAT‑Aa‑aaa                                                                                                                               >  2:1522224/1‑140 (MQ=255)

          tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑TTTTTTTATTTA‑A‑TT‑AAT‑Aa‑aaa                                                                                                                               >  1:2159892/1‑140 (MQ=255)

          tACTAGGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑ATTTTTTTTTTA‑A‑TT‑TCT‑AC‑aca                                                                                                                               >  2:354647/1‑138 (MQ=255)

          tACAATGATTGGCTGAACCGGCTTGCTGAATAGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTTAAACAAAGTAAAACAAAAAAGCCTGTGAAACCTGTCGT‑TTTTTTTTT‑ATTTTTTATTTA‑A‑Tt‑aaaaaaaa                                                                                                                                 >  2:1972635/1‑133 (MQ=255)

           aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGTAACAAAGTAAATCAAAAACGCCTGGGAAACCGGGCGT‑GGTTTTTTT‑GGTTTTGTTGTT‑A‑AT‑ACT‑Aa‑aaaa                                                                                                                              >  1:387956/1‑139 (MQ=255)

           aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑ATTTTTTATGTA‑A‑TT‑ACA‑AC‑AAA‑t                                                                                                                             >  2:1847936/1‑140 (MQ=255)

               tGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑GTTTTTTATTTA‑A‑TT‑AAT‑AA‑AAA‑tttta                                                                                                                         >  2:372864/1‑138 (MQ=255)

                 aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTTTT‑CTTTTTTTTTTA‑A‑TT‑AAT‑AA‑AAA‑tttttaa                                                                                                                       >  2:1254296/1‑136 (MQ=255)

                  ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGAGTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑TTTTTTTATGTT‑A‑TT‑TAT‑AC‑AAA‑ATTG‑ataa                                                                                                                     >  1:3330263/1‑140 (MQ=255)

                    ggatGAAACGGATAGCTCAATCGCTGCACAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAAAAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAAt                                                                                                                   <  1:3380145/137‑1 (MQ=255)

                    ggCTTACACGGCTTTGTGAATCGGTGCAGAGCGGTGAAATTTTTAAATTTATACGGATTTAAACAAAGTAAACAAAAACCGCCTTGGAACCCGGGCGT‑TTTTTTGTT‑ATTTTTTATGTA‑A‑TT‑AGT‑AC‑AAA‑TTTT‑AAAAAt                                                                                                                   <  2:773954/140‑1 (MQ=255)

                    ggCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑GTTTTTTTTGTA‑A‑AT‑TCT‑AA‑AAA‑TTTG‑AAAAAt                                                                                                                   >  1:1794431/1‑140 (MQ=255)

                    ggCTGAAACGGCTTGCTGAATCGGTGCAGAGCGCGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAAt                                                                                                                   <  2:3506316/140‑1 (MQ=255)

                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑TTTTTTTTTTTT‑A‑AT‑AAT‑AA‑AAA‑TATT‑ATAAATa                                                                                                                  >  1:1439324/1‑140 (MQ=255)

                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑TGTTTTGATTTA‑A‑AT‑TGT‑AA‑AAA‑ATTT‑AAAAATa                                                                                                                  >  1:2065124/1‑140 (MQ=255)

                       tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑ATTTTTTATTTA‑A‑TT‑AAT‑AC‑AAA‑ATTG‑AAAAATaag                                                                                                                >  2:626202/1‑138 (MQ=255)

                       tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑ATTTTTTATGTA‑A‑TT‑AAT‑AC‑AAA‑TTTG‑AAAAATaag                                                                                                                >  2:3981835/1‑138 (MQ=255)

                       tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑TTTTTTTTTTTA‑ATTT‑AAA‑AA‑AAA‑TTTT‑AAAAAAAc                                                                                                                 >  1:156956/1‑140 (MQ=255)

                       tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑GTTTTTTTTTTT‑A‑TT‑AATAAA‑AAA‑TTTT‑aaaaaaag                                                                                                                 >  1:203360/1‑137 (MQ=255)

                       tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGGT‑ATATTTTATTTA‑A‑TT‑TAT‑AA‑AAA‑ATTT‑aaaaaaaag                                                                                                                >  1:2630618/1‑136 (MQ=255)

                        gAAACGTCTTGCTGAATCGGTGCATAGCGGTGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGTCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACgg                                                                                                               <  2:756913/140‑1 (MQ=255)

                               cTTGCTGAATCGGTGCATAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TGTTGTGTT‑GGTTTTTTTTTT‑A‑AT‑ACTAAA‑AAA‑TTTT‑AAAAAAACGGaaaaaa                                                                                                         >  1:568115/1‑140 (MQ=255)

                               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTTGTT‑‑TTTTTTTTTTT‑A‑TT‑ATT‑AA‑AAATTTTA‑AAAAAAACGGTaaaaa                                                                                                         >  1:175633/1‑140 (MQ=255)

                               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTTTT‑GTTTTTTATGTA‑A‑TT‑AAT‑AA‑AAA‑TTTT‑AAAAAAAAGGGAAAAAc                                                                                                        >  2:1282907/1‑140 (MQ=255)

                               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑TTTTTTTTTTTA‑T‑TT‑AAT‑TA‑AAA‑TTTGTAAAAAAATGGGaaaaa                                                                                                         >  1:377487/1‑140 (MQ=255)

                               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑GTTTTTTTTTTT‑A‑TTAAAA‑AA‑AAA‑TTTT‑AAAAAAAAGGaaaaaa                                                                                                         >  1:2269333/1‑140 (MQ=255)

                               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTTGTTTTTTTTTTTA‑A‑TT‑A‑G‑AC‑AAA‑TTTT‑TAAAATACgggctaaaa                                                                                                        >  1:3013933/1‑134 (MQ=255)

                               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑GTTTTTTTTTTA‑A‑AT‑AAT‑AC‑AAA‑ATTT‑AAAAAAAAGGGaaaaaa                                                                                                        >  1:2367297/1‑139 (MQ=255)

                                ttGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑GTTTTTTATTTT‑A‑TT‑AAT‑AC‑AAA‑ATTT‑TAAAAAAAGGGaaaaaaa                                                                                                       >  2:1557897/1‑138 (MQ=255)

                                        tCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑GTTTTTTATGTT‑A‑TT‑AAT‑AA‑AAA‑ATTG‑TAAAATACTGGAAAAAAATCCCAttt                                                                                               >  2:3429076/1‑140 (MQ=255)

                                        tCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑GTTTTTTTTTTT‑A‑TT‑TAA‑AA‑AAA‑ATTT‑AAAAATACAGGGTAAACATACCAatt                                                                                               >  1:3322861/1‑140 (MQ=255)

                                        tCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑GTTTTTTTTGTT‑A‑TT‑AAT‑AA‑AAA‑TTTT‑TAAAATAAGGGAAAAAAAAccccatt                                                                                               >  1:647912/1‑136 (MQ=255)

                                            tGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑GTTTGTTATTTA‑A‑TT‑AAT‑AC‑AAA‑TTTG‑AAAAATACGGGCAAAAGATCCCATGTagtg                                                                                           >  2:2331616/1‑137 (MQ=255)

                                                gagCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTGTTTGTT‑GTTTTTTATTTA‑ATTT‑AGA‑AA‑AAA‑GTTG‑‑AAAATAGGGGGAAAAAATACCAATTAtttacca                                                                                       >  1:3640872/1‑136 (MQ=255)

                                                 agCGGGTAAATTTTTAAATTTATACCTATTTAAAAAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCaaa                                                                                      <  1:2716908/140‑1 (MQ=255)

                                                 agCGGGGAAATTTTTAAATTTATACCGATTTAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCaaa                                                                                      <  2:2592285/140‑1 (MQ=255)

                                                 agCGGGCAAATTTTTAAATTAATACCTATTCAAACAAAGAAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCaaa                                                                                      <  1:2535329/140‑1 (MQ=255)

                                                     gggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGGT‑GTTTTTTATTTA‑A‑TT‑TCT‑AC‑AAA‑TTTT‑AAAAATACGGGAAAAACATACCAGTTACTACCaaaaatt                                                                                  >  2:139498/1‑137 (MQ=255)

                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGACACCGGGCGT‑GTTTTTGTT‑GGTTTTTATGTA‑A‑GT‑TAT‑AC‑AAA‑TATG‑AAAAATACGGGAAAAACATACCAAGTACTTCCAAAAGAtt                                                                                 >  2:518044/1‑140 (MQ=255)

                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑GTTTTTTTTTTA‑A‑TT‑AAT‑AA‑AAA‑TTTG‑AAAAATAAGGGAAAAACAACCCATTTACTTGCAAAAAAtt                                                                                 >  2:3350669/1‑140 (MQ=255)

                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑GTTTTTTATGTT‑A‑TT‑AAT‑AC‑AAA‑TTTT‑AAAAATACGGGAAAAACATCACCTGTTGTTACAAAATAtt                                                                                 >  2:1500378/1‑140 (MQ=255)

                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GGTTTTGTT‑GTTTTTTTTGTT‑A‑TT‑TAA‑AA‑AAA‑TTTT‑TAAAATAAGCGGAAAAAATACCCTTTTATTCCaaaaaaat                                                                                 >  1:3503818/1‑136 (MQ=255)

                                                      ggAAATTTTTAAAGTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑GTTTTTTATGTA‑A‑TT‑AAT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAAAAACACATTTACTTACACAATAtt                                                                                 >  2:368663/1‑140 (MQ=255)

                                                         aaTTTTTAAATTTATACCTATTCAAACAAAGTAAAAAAAAAACGCCTGGGAAACCGGGAGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGa                                                                              <  1:3028563/140‑1 (MQ=255)

                                                         aaTTTTTAAATTTATACCGATTTAAAAAAATAAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGa                                                                              <  1:1522224/140‑1 (MQ=255)

                                                           tttttAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATg                                                                            <  2:434557/140‑1 (MQ=255)

                                                            ttttACATTTATCACTATTGAAACAAATAAAACCACAAACGCCTGGGAAACCCGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACG‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                           <  2:2532639/140‑1 (MQ=255)

                                                            ttttAAATTTATACCGATTGAAACAAAGTAAACCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAACATATTTGATgg                                                                           <  2:237824/140‑1 (MQ=255)

                                                                           gATTGAAACAAAGTAAAACAAACACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                            <  2:424473/140‑1 (MQ=255)

                                                                           gATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                            <  2:2250199/140‑1 (MQ=255)

                                                                           gATTCAAAAAAAGTAAAACAAACACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                            <  1:1521837/140‑1 (MQ=255)

                                                                            aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑GTTTTTTATGTA‑A‑TT‑AAA‑AA‑AAA‑TTTG‑AAAAATAAGGGAAAAAAATCCCCTTTTGATGCAAAATATTTGTTTGTAATTTTTTGTAggga                                                           >  2:3349894/1‑138 (MQ=255)

                                                                            aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑GATTTTTATTTATT‑TT‑AAA‑AA‑AAA‑GTTA‑AAAAAAATGGGAAAAA‑ATACCCAATAATTTGAAAATATATTATTTTAATTGTATGTAGTTa                                                           >  1:1841927/1‑140 (MQ=255)

                                                                            aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑GTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑ATTG‑AAAAAAAAGGGAAAAACATCCCAGTTTCTGCCAAAAAGTTTGATGGTAATTTTTTGTgggga                                                           >  2:3449591/1‑138 (MQ=255)

                                                                            aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑GTTTTTTATGTA‑A‑TT‑ACA‑AC‑AAA‑ATTG‑AAAAATAAGGGAAAAACATACCATTTACTTCCCAAATATTTGATGGTAATTTTATGTAGGTa                                                           >  2:174742/1‑140 (MQ=255)

                                                                            aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑AAT‑ACAAAA‑TTTT‑AAAAAAAC‑GGAAAAACATAACAATTAAGTCCAAAATATTTTATTGTAATTTTATTTATGTa                                                           >  2:273471/1‑140 (MQ=255)

                                                                              tGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTTTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AA‑AAA‑ATTT‑AAAAAAACGGGGAAAAAAACCCAATTACTTACAAAATATTTGATGGTCATTTTATGTAGGTaaa                                                         >  2:3116327/1‑140 (MQ=255)

                                                                                aaaaaaACTAAAACAAACACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                       <  1:3429076/140‑1 (MQ=255)

                                                                                aaaCAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                       <  2:352083/140‑1 (MQ=255)

                                                                                     aactAAAACAAAAACGCCTGGGAAACCGGGCGA‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTa                                                  <  2:2769626/137‑1 (MQ=255)

                                                                                      aGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑CTTTTTTAAGTA‑A‑TT‑ACT‑AA‑AAA‑TTTT‑TAAAATACTTGAAAAAAATCCCAATTACTTACAAAAAAATTGATTGTAATTTTATGTTGGGAAAAtttttta                                                 >  2:3816497/1‑136 (MQ=255)

                                                                                          aaccAAACACGCCTGGCACACCGGGCGA‑ATTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                             <  1:3676476/137‑1 (MQ=255)

                                                                                          aacactaaaCGCGTGGGAAACCGGTCGT‑TTTTTTGTT‑ATTTTTTATGTT‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAATTATTTGATGGTAATTTTATGTAGGTCAAATTTGTATTTgg                                             <  1:3350741/134‑1 (MQ=255)

                                                                                          aaacaaacACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                             <  1:168576/140‑1 (MQ=255)

                                                                                          aaacaaacACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                             <  1:1368729/140‑1 (MQ=255)

                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                             <  1:3676501/140‑1 (MQ=255)

                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                             <  1:3350669/140‑1 (MQ=255)

                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                             <  2:2630618/140‑1 (MQ=255)

                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                             <  1:448919/140‑1 (MQ=255)

                                                                                                  aCGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCag                                     <  1:753712/140‑1 (MQ=255)

                                                                                                  aCGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCag                                     <  1:1758289/140‑1 (MQ=255)

                                                                                                    gCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                   <  1:100833/140‑1 (MQ=255)

                                                                                                    gCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                   <  1:100663/140‑1 (MQ=255)

                                                                                                    gCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                   <  1:220968/140‑1 (MQ=255)

                                                                                                    gCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                   <  1:2730824/140‑1 (MQ=255)

                                                                                                    gCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                   <  1:3981835/140‑1 (MQ=255)

                                                                                                    gCCTGGGAAACCGGGAGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTGGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                   <  1:2569756/140‑1 (MQ=255)

                                                                                                              ccGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaaa                         <  1:1500378/140‑1 (MQ=255)

                                                                                                                    gT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGc                   <  2:2443697/140‑1 (MQ=255)

                                                                                                                    gT‑TTTTTTGTT‑ATTTTTTATTTA‑A‑TT‑AAT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGc                   <  2:895151/140‑1 (MQ=255)

                                                                                                                     t‑ttttttGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑CA‑AAA‑TTTG‑AAAAATACGGGAAAAAAATAACCTTTTCTTGCAAAAAATTTGATGGTAAATTTATGTAGGTAAAAATTTTATTTTGGCGTTCAGAGGCGGGGAAAAGCcgggc                  >  2:3084458/1‑138 (MQ=255)

                                                                                                                     t‑ttttttGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCa                  <  2:922274/140‑1 (MQ=255)

                                                                                                                         ttttGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAC‑TGTG‑AAAAATACGGGAACAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGc               <  2:2272442/140‑1 (MQ=255)

                                                                                                                         ttttGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACCTCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGc               <  1:1958713/140‑1 (MQ=255)

                                                                                                                         ttttGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGc               <  2:160840/140‑1 (MQ=255)

                                                                                                                         ttttGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGc               <  2:1698132/140‑1 (MQ=255)

                                                                                                                         ttttGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGc               <  2:2272438/140‑1 (MQ=255)

                                                                                                                         ttttGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGc               <  2:2381995/140‑1 (MQ=255)

                                                                                                                         ttttGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACCTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGc               <  2:2447282/140‑1 (MQ=255)

                                                                                                                               t‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAat         <  2:3396556/140‑1 (MQ=255)

                                                                                                                                tattttttATTTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAat         <  2:175633/138‑1 (MQ=255)

                                                                                                                                tattttttATTTA‑A‑TT‑AAT‑AA‑AAA‑TTTG‑AAAAATACGGGAAAAAAATCACATTTACTTCCAAAATATTTGATGGTAATTTTATGTATTTAAAATTTTTATTTTGGTTTTAATTTTAGGTTAAAAGCGGGCAGGACttatt         <  1:2579427/138‑5 (MQ=255)

                                                                                                                                       tATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTta  <  2:853010/140‑1 (MQ=255)

CTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTA  >  NZ_CP034943/2991109‑2991381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`