BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,991,252	C→A	36.7%	intergenic (+53/‑154)	gbsR → / → bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,991,252	0	C	A	36.7%	114.5 / 35.7	111	intergenic (+53/‑154)	gbsR/bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand): ref base C (13/51); new base A (40/2); total (57/54)
Fisher's exact test for biased strand distribution p-value = 3.65e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

TACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTT  >  NZ_CP034943/2991119‑2991390

tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTTTT‑GTTTTTTTTTTA‑A‑AT‑AAT‑Aa‑aaa                                                                                                                                        >  1:516705/1‑140 (MQ=255)

tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTTTT‑GTTTTTTATTTA‑A‑TT‑AAT‑AC‑aaa                                                                                                                                        >  2:753712/1‑140 (MQ=255)

tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTTTT‑CTTTTTTATGTA‑A‑TT‑AAT‑AC‑aaa                                                                                                                                        >  2:1043605/1‑140 (MQ=255)

tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑GTTTTTTATTTA‑A‑TA‑ACT‑AC‑aaa                                                                                                                                        >  2:754100/1‑140 (MQ=255)

tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑AAT‑Aa‑aaa                                                                                                                                        >  2:3178608/1‑140 (MQ=255)

tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑GTTTTTTTTTTT‑A‑TT‑AAT‑Aa‑aaa                                                                                                                                        >  1:1699397/1‑140 (MQ=255)

tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑GTTTTTTATTTA‑A‑TT‑AAT‑Aa‑aaa                                                                                                                                        >  2:1522224/1‑140 (MQ=255)

tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTGT‑GTTTTTTTTTTA‑A‑TT‑AAT‑Aa‑aaa                                                                                                                                        >  1:281759/1‑140 (MQ=255)

tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑TTTTTTTATTTA‑A‑TT‑AAT‑Aa‑aaa                                                                                                                                        >  1:2159892/1‑140 (MQ=255)

 aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑ATTTTTTATGTA‑A‑TT‑ACA‑AC‑AAA‑t                                                                                                                                      >  2:1847936/1‑140 (MQ=255)

     tGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑GTTTTTTATTTA‑A‑TT‑AAT‑AA‑AAA‑tttta                                                                                                                                  >  2:372864/1‑138 (MQ=255)

        ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TGTTTTTTT‑GTTTTTTTTTTA‑A‑TT‑AAT‑AC‑AAA‑attttaaa                                                                                                                               >  1:3858678/1‑135 (MQ=255)

        ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGAGTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑TTTTTTTATGTT‑A‑TT‑TAT‑AC‑AAA‑ATTG‑ataa                                                                                                                              >  1:3330263/1‑140 (MQ=255)

          ggCTTACACGGCTTTGTGAATCGGTGCAGAGCGGTGAAATTTTTAAATTTATACGGATTTAAACAAAGTAAACAAAAACCGCCTTGGAACCCGGGCGT‑TTTTTTGTT‑ATTTTTTATGTA‑A‑TT‑AGT‑AC‑AAA‑TTTT‑AAAAAt                                                                                                                            <  2:773954/140‑1 (MQ=255)

          ggCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTGT‑GTTTTTTATTTA‑A‑TT‑AAT‑AC‑AAA‑ATTT‑aaaaaa                                                                                                                            >  1:1558864/1‑139 (MQ=255)

          ggCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑GTTTTTTTTGTA‑A‑AT‑TCT‑AA‑AAA‑TTTG‑AAAAAt                                                                                                                            >  1:1794431/1‑140 (MQ=255)

          ggCTGAAACGGCTTGCTGAATCGGTGCAGAGCGCGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAAt                                                                                                                            <  2:3506316/140‑1 (MQ=255)

           gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑TTTTTTTTTTTT‑A‑AT‑AAT‑AA‑AAA‑TATT‑ATAAATa                                                                                                                           >  1:1439324/1‑140 (MQ=255)

           gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑GGTTTTTTTGTT‑A‑TT‑AAT‑AC‑CAA‑TTTT‑taaaaaa                                                                                                                           >  1:1858018/1‑138 (MQ=255)

             tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑ATTTTTTATTTA‑A‑TT‑AAT‑AC‑AAA‑ATTG‑AAAAATaag                                                                                                                         >  2:626202/1‑138 (MQ=255)

             tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑ATTTTTTATGTA‑A‑TT‑AAT‑AC‑AAA‑TTTG‑AAAAATaag                                                                                                                         >  2:3981835/1‑138 (MQ=255)

             tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑TTTTTTTTTTTA‑ATTT‑AAA‑AA‑AAA‑TTTT‑AAAAAAAc                                                                                                                          >  1:156956/1‑140 (MQ=255)

             tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑GTTTTTTTTTTT‑A‑TT‑AATAAA‑AAA‑TTTT‑aaaaaaag                                                                                                                          >  1:203360/1‑137 (MQ=255)

              gAAACGTCTTGCTGAATCGGTGCATAGCGGTGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGTCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACgg                                                                                                                        <  2:756913/140‑1 (MQ=255)

                     cTTGCTGAATCGGTGCATAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TGTTGTGTT‑GGTTTTTTTTTT‑A‑AT‑ACTAAA‑AAA‑TTTT‑AAAAAAACGGaaaaaa                                                                                                                  >  1:568115/1‑140 (MQ=255)

                     cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTTGTT‑‑TTTTTTTTTTT‑A‑TT‑ATT‑AA‑AAATTTTA‑AAAAAAACGGTaaaaa                                                                                                                  >  1:175633/1‑140 (MQ=255)

                     cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTTTT‑GTTTTTTATGTA‑A‑TT‑AAT‑AA‑AAA‑TTTT‑AAAAAAAAGGGAAAAAc                                                                                                                 >  2:1282907/1‑140 (MQ=255)

                     cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑TTTTTTTTTTTA‑T‑TT‑AAT‑TA‑AAA‑TTTGTAAAAAAATGGGaaaaa                                                                                                                  >  1:377487/1‑140 (MQ=255)

                     cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑GTTTTTTTTTTT‑A‑TTAAAA‑AA‑AAA‑TTTT‑AAAAAAAAGGaaaaaa                                                                                                                  >  1:2269333/1‑140 (MQ=255)

                     cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑GTTTTTTATTTT‑A‑TT‑AAT‑AC‑AAA‑ATTT‑AAAAATAACGGacaaaa                                                                                                                 >  1:3396556/1‑139 (MQ=255)

                     cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTTGTTTTTTTTTTTA‑A‑TT‑A‑G‑AC‑AAA‑TTTT‑TAAAATACgggctaaaa                                                                                                                 >  1:3013933/1‑134 (MQ=255)

                     cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑GTTTTTTTTTTA‑A‑AT‑AAT‑AC‑AAA‑ATTT‑AAAAAAAAGGGaaaaaa                                                                                                                 >  1:2367297/1‑139 (MQ=255)

                      ttGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑GTTTTTTATTTT‑A‑TT‑AAT‑AC‑AAA‑ATTT‑TAAAAAAAGGGaaaaaaa                                                                                                                >  2:1557897/1‑138 (MQ=255)

                              tCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑GTTTTTTATGTT‑A‑TT‑AAT‑AA‑AAA‑ATTG‑TAAAATACTGGAAAAAAATCCCAttt                                                                                                        >  2:3429076/1‑140 (MQ=255)

                              tCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑GTTTTTTTTTTT‑A‑TT‑TAA‑AA‑AAA‑ATTT‑AAAAATACAGGGTAAACATACCAatt                                                                                                        >  1:3322861/1‑140 (MQ=255)

                              tCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑GTTTTTTTTGTT‑A‑TT‑AAT‑AA‑AAA‑TTTT‑TAAAATAAGGGAAAAAAAAccccatt                                                                                                        >  1:647912/1‑136 (MQ=255)

                                  tGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑GTTTGTTATTTA‑A‑TT‑AAT‑AC‑AAA‑TTTG‑AAAAATACGGGCAAAAGATCCCATGTagtg                                                                                                    >  2:2331616/1‑137 (MQ=255)

                                      gagCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTGTTTGTT‑GTTTTTTATTTA‑ATTT‑AGA‑AA‑AAA‑GTTG‑‑AAAATAGGGGGAAAAAATACCAATTAtttacca                                                                                                >  1:3640872/1‑136 (MQ=255)

                                       agCGGGTAAATTTTTAAATTTATACCTATTTAAAAAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCaaa                                                                                               <  1:2716908/140‑1 (MQ=255)

                                       agCGGGGAAATTTTTAAATTTATACCGATTTAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCaaa                                                                                               <  2:2592285/140‑1 (MQ=255)

                                       agCGGGCAAATTTTTAAATTAATACCTATTCAAACAAAGAAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCaaa                                                                                               <  1:2535329/140‑1 (MQ=255)

                                           gggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGGT‑GTTTTTTATTTA‑A‑TT‑TCT‑AC‑AAA‑TTTT‑AAAAATACGGGAAAAACATACCAGTTACTACCaaaaatt                                                                                           >  2:139498/1‑137 (MQ=255)

                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACTCCTGGGAAACCGGGCGT‑GTTTTTGTT‑ATTTTTTATGTA‑A‑AT‑AAT‑AC‑AAA‑ATTG‑AAAAAAACGGGAAAAACAAACCAATTAAGTACAAAatagt                                                                                          >  2:3350741/1‑138 (MQ=255)

                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑GTTTTTTTTTTA‑A‑TT‑AAT‑AA‑AAA‑TTTG‑AAAAATAAGGGAAAAACAACCCATTTACTTGCAAAAAAtt                                                                                          >  2:3350669/1‑140 (MQ=255)

                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑GTTTTTTATGTT‑A‑TT‑AAT‑AC‑AAA‑TTTT‑AAAAATACGGGAAAAACATCACCTGTTGTTACAAAATAtt                                                                                          >  2:1500378/1‑140 (MQ=255)

                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GGTTTTGTT‑GTTTTTTTTGTT‑A‑TT‑TAA‑AA‑AAA‑TTTT‑TAAAATAAGCGGAAAAAATACCCTTTTATTCCaaaaaaat                                                                                          >  1:3503818/1‑136 (MQ=255)

                                            ggAAATTTTTAAAGTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑GTTTTTTATGTA‑A‑TT‑AAT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAAAAACACATTTACTTACACAATAtt                                                                                          >  2:368663/1‑140 (MQ=255)

                                            ggAAATTTTTAAAGTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑AAT‑AC‑ACA‑ATTG‑AAAAATAAGGGAAAAACAACCCATTTTATTCCAAAATAtt                                                                                          >  2:369010/1‑140 (MQ=255)

                                               aaTTTTTAAATTTATACCTATTCAAACAAAGTAAAAAAAAAACGCCTGGGAAACCGGGAGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGa                                                                                       <  1:3028563/140‑1 (MQ=255)

                                               aaTTTTTAAATTTATACCGATTTAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGa                                                                                       <  1:2291165/140‑1 (MQ=255)

                                               aaTTTTTAAATTTATACCGATTTAAAAAAATAAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGa                                                                                       <  1:1522224/140‑1 (MQ=255)

                                                 tttttAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATg                                                                                     <  2:434557/140‑1 (MQ=255)

                                                  ttttACATTTATCACTATTGAAACAAATAAAACCACAAACGCCTGGGAAACCCGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACG‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                                    <  2:2532639/140‑1 (MQ=255)

                                                  ttttAAATTTATACCGATTGAAACAAAGTAAACCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAACATATTTGATgg                                                                                    <  2:237824/140‑1 (MQ=255)

                                                                 gATTGAAACAAAGTAAAACAAACACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                                     <  2:424473/140‑1 (MQ=255)

                                                                 gATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                                     <  2:2250199/140‑1 (MQ=255)

                                                                 gATTCAAAAAAAGTAAAACAAACACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                                     <  1:1521837/140‑1 (MQ=255)

                                                                  aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑GTTTTTTATGTA‑A‑TT‑AAA‑AA‑AAA‑TTTG‑AAAAATAAGGGAAAAAAATCCCCTTTTGATGCAAAATATTTGTTTGTAATTTTTTGTAggga                                                                    >  2:3349894/1‑138 (MQ=255)

                                                                  aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑GATTTTTATTTATT‑TT‑AAA‑AA‑AAA‑GTTA‑AAAAAAATGGGAAAAA‑ATACCCAATAATTTGAAAATATATTATTTTAATTGTATGTAGTTa                                                                    >  1:1841927/1‑140 (MQ=255)

                                                                  aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑GTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑ATTG‑AAAAAAAAGGGAAAAACATCCCAGTTTCTGCCAAAAAGTTTGATGGTAATTTTTTGTgggga                                                                    >  2:3449591/1‑138 (MQ=255)

                                                                  aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑GTTTTTTATGTA‑A‑TT‑ACA‑AC‑AAA‑ATTG‑AAAAATAAGGGAAAAACATACCATTTACTTCCCAAATATTTGATGGTAATTTTATGTAGGTa                                                                    >  2:174742/1‑140 (MQ=255)

                                                                  aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑AAT‑ACAAAA‑TTTT‑AAAAAAAC‑GGAAAAACATAACAATTAAGTCCAAAATATTTTATTGTAATTTTATTTATGTa                                                                    >  2:273471/1‑140 (MQ=255)

                                                                      aaaaaaACTAAAACAAACACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                                <  1:3429076/140‑1 (MQ=255)

                                                                      aaaCAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                                <  2:352083/140‑1 (MQ=255)

                                                                           aactAAAACAAAAACGCCTGGGAAACCGGGCGA‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTa                                                           <  2:2769626/137‑1 (MQ=255)

                                                                            aGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑CTTTTTTAAGTA‑A‑TT‑ACT‑AA‑AAA‑TTTT‑TAAAATACTTGAAAAAAATCCCAATTACTTACAAAAAAATTGATTGTAATTTTATGTTGGGAAAAtttttta                                                          >  2:3816497/1‑136 (MQ=255)

                                                                                aaccAAACACGCCTGGCACACCGGGCGA‑ATTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  1:3676476/137‑1 (MQ=255)

                                                                                aacactaaaCGCGTGGGAAACCGGTCGT‑TTTTTTGTT‑ATTTTTTATGTT‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAATTATTTGATGGTAATTTTATGTAGGTCAAATTTGTATTTgg                                                      <  1:3350741/134‑1 (MQ=255)

                                                                                aaacaaacACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  1:1368729/140‑1 (MQ=255)

                                                                                aaacaaacACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  2:156956/140‑1 (MQ=255)

                                                                                aaaCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  1:3676501/140‑1 (MQ=255)

                                                                                aaaCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  2:2630618/140‑1 (MQ=255)

                                                                                aaaCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  2:203360/140‑1 (MQ=255)

                                                                                aaaCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  1:3350669/140‑1 (MQ=255)

                                                                                aaaCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  1:448919/140‑1 (MQ=255)

                                                                                        aCGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCag                                              <  1:1758289/140‑1 (MQ=255)

                                                                                        aCGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCag                                              <  1:754100/140‑1 (MQ=255)

                                                                                        aCGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCag                                              <  1:753712/140‑1 (MQ=255)

                                                                                          gCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                            <  1:100833/140‑1 (MQ=255)

                                                                                          gCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                            <  1:100663/140‑1 (MQ=255)

                                                                                          gCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                            <  1:220968/140‑1 (MQ=255)

                                                                                          gCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                            <  1:2730824/140‑1 (MQ=255)

                                                                                          gCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                            <  1:3981835/140‑1 (MQ=255)

                                                                                          gCCTGGGAAACCGGGAGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTGGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                            <  1:2569756/140‑1 (MQ=255)

                                                                                                    ccGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaaa                                  <  1:1500378/140‑1 (MQ=255)

                                                                                                          gT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGc                            <  2:2443697/140‑1 (MQ=255)

                                                                                                          gT‑TTTTTTGTT‑ATTTTTTATTTA‑A‑TT‑AAT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGc                            <  2:895151/140‑1 (MQ=255)

                                                                                                           t‑ttttttGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑CA‑AAA‑TTTG‑AAAAATACGGGAAAAAAATAACCTTTTCTTGCAAAAAATTTGATGGTAAATTTATGTAGGTAAAAATTTTATTTTGGCGTTCAGAGGCGGGGAAAAGCcgggc                           >  2:3084458/1‑138 (MQ=255)

                                                                                                           t‑ttttttGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCa                           <  2:922274/140‑1 (MQ=255)

                                                                                                               ttttGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAC‑TGTG‑AAAAATACGGGAACAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGc                        <  2:2272442/140‑1 (MQ=255)

                                                                                                               ttttGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACCTCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGc                        <  1:1958713/140‑1 (MQ=255)

                                                                                                               ttttGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGc                        <  2:160840/140‑1 (MQ=255)

                                                                                                               ttttGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGc                        <  2:1698132/140‑1 (MQ=255)

                                                                                                               ttttGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGc                        <  2:2272438/140‑1 (MQ=255)

                                                                                                               ttttGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGc                        <  2:2381995/140‑1 (MQ=255)

                                                                                                               ttttGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACCTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGc                        <  2:2447282/140‑1 (MQ=255)

                                                                                                                     t‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAat                  <  2:3396556/140‑1 (MQ=255)

                                                                                                                      tattttttATTTA‑A‑TT‑AAT‑AA‑AAA‑TTTG‑AAAAATACGGGAAAAAAATCACATTTACTTCCAAAATATTTGATGGTAATTTTATGTATTTAAAATTTTTATTTTGGTTTTAATTTTAGGTTAAAAGCGGGCAGGACttatt                  <  1:2579427/138‑5 (MQ=255)

                                                                                                                             tATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTta           <  2:853010/140‑1 (MQ=255)

                                                                                                                                         act‑ac‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACttttt  >  2:398150/1‑140 (MQ=255)

TACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTATGTA‑A‑TT‑ACT‑AC‑AAA‑TTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTT  >  NZ_CP034943/2991119‑2991390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`