BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,285,713	G→A	28.5%	L230L (CTA→TTA)	EO946_RS16995 ←	IS3 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,285,713	0	G	A	28.5%	495.6 / 241.5	303	L230L (CTA→TTA)	EO946_RS16995	IS3 family transposase
Reads supporting (aligned to +/- strand): ref base G (82/133); new base A (57/29); total (141/162)
Fisher's exact test for biased strand distribution p-value = 1.18e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

Only 100 of 303 total aligned reads displayed.

ATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCATT  >  NZ_CP034943/3285580‑3285785

gtGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTc                                                                    >  2:3891565/2‑140 (MQ=16)

aTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTc                                                                    >  1:569415/1‑140 (MQ=40)

 tGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCa                                                                   >  1:1789200/1‑140 (MQ=21)

   tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTCCAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAag                                                                 >  2:903759/1‑140 (MQ=255)

   tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAag                                                                 >  2:1817844/1‑140 (MQ=40)

   tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAag                                                                 >  1:991545/1‑140 (MQ=40)

   tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAag                                                                 >  1:1443156/1‑140 (MQ=40)

   tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAag                                                                 >  1:3953867/1‑140 (MQ=40)

   tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAag                                                                 >  1:3820034/1‑140 (MQ=40)

   tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAag                                                                 >  2:3865084/1‑140 (MQ=40)

   tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCa                                                                   >  2:2902334/1‑138 (MQ=21)

   tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAAGTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAag                                                                 >  1:3379986/1‑140 (MQ=255)

         aCAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCg                                                           >  1:3289295/1‑140 (MQ=21)

           aaaGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTTTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGAt                                                         <  2:1213762/140‑1 (MQ=255)

           aaaGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGAt                                                         <  2:1747707/140‑1 (MQ=21)

           aaaGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGAt                                                         <  1:3887000/140‑1 (MQ=21)

           aaaGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGAt                                                         <  2:2653574/140‑1 (MQ=21)

           aaaGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGAt                                                         <  2:713623/140‑1 (MQ=21)

             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAATGTACAATTGTTTCTGTCCATAAGTCACATATGTGATTTCAGTTACTAGTTTTACAGGAGGGAGATCa                                                       >  2:3071947/1‑140 (MQ=14)

             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATCAGGCAAATATGTGATGTCAGTTACTCTTTTTTCAAGAGTGCGATCa                                                       >  1:4005395/1‑140 (MQ=14)

             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCa                                                                   >  1:2810395/1‑128 (MQ=40)

             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCa                                                                   <  2:2810395/128‑1 (MQ=40)

             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCTAGAGGGCGATCa                                                       >  2:1958976/1‑140 (MQ=255)

             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGATGGCGATCa                                                       >  2:220196/1‑140 (MQ=255)

             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                       >  1:1478183/1‑140 (MQ=40)

             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                       >  1:139678/1‑140 (MQ=40)

             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                       >  2:2317506/1‑140 (MQ=40)

             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                       >  1:1699457/1‑140 (MQ=40)

             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                       >  2:1928772/1‑140 (MQ=40)

             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                       >  1:1256543/1‑140 (MQ=40)

             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                       >  2:1980864/1‑140 (MQ=40)

             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                       >  2:3145964/1‑140 (MQ=40)

             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                       >  2:1160588/1‑140 (MQ=21)

             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                       >  2:1464784/1‑140 (MQ=21)

             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                       >  2:1348783/1‑140 (MQ=21)

             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                       >  2:2525028/1‑140 (MQ=21)

             aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTAGATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATTTGATGTCAGTTACTAGTTTTTCAAGAGTGCGAtaa                                                       >  2:3366411/1‑138 (MQ=14)

               tCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGa                                                     <  1:2586390/140‑1 (MQ=21)

                   tgtCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGa                                                 >  1:1972385/1‑140 (MQ=40)

                   tgtCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGa                                                 <  1:3681946/140‑1 (MQ=40)

                   tgtCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGTGCGATCAGACTGa                                                 >  1:3043086/1‑140 (MQ=255)

                   tgtCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGa                                                 >  1:2451965/1‑140 (MQ=21)

                   tgtCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGa                                                 >  1:1004861/1‑140 (MQ=21)

                   tgtCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGa                                                 >  1:806788/1‑140 (MQ=21)

                   tgtCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGa                                                 >  1:838807/1‑140 (MQ=21)

                   tgtCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGa                                                 >  1:3002124/1‑140 (MQ=21)

                   ggtCCTGCTTATCCCCAATCGAAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCGGTTTCTAATTTTTCAAGAGGGCGATCAGAGTGa                                                 >  1:632037/2‑140 (MQ=11)

                         gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTc                                           <  1:1550981/140‑1 (MQ=40)

                         gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTGTCTGTCCATAAGGCAAATATGTGATGTCCGTTGCTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTc                                           <  2:3720719/140‑1 (MQ=255)

                          cTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTcc                                          >  1:3755235/1‑140 (MQ=21)

                           ttATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCg                                         <  1:747503/140‑1 (MQ=40)

                           ttATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCg                                         <  1:685725/140‑1 (MQ=40)

                           ttATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCg                                         <  1:3444678/140‑1 (MQ=40)

                           ttATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCg                                         <  2:815323/140‑1 (MQ=40)

                           ttATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCg                                         <  2:2728296/140‑1 (MQ=21)

                           ttATCCCCAATCGTAAAAGCAATCACTTCTCCATGGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCg                                         <  2:3802505/140‑1 (MQ=255)

                             aTCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGAt                                       >  1:2243890/1‑140 (MQ=18)

                              tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                      <  2:1552518/140‑1 (MQ=17)

                              tCCCCAATCGTAAAAGCAATCAATTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                      <  2:1122281/140‑1 (MQ=12)

                              tCCCCAATCGTAAAAGAAATCACTTCTCCATTGTATAAA‑TCTAATATACTTGAAAGTTACAATTGTTTCTGTACATAAGGCAAATATGTGATGTCGTTTACTAATTTTTCAAGAGGGCGATGAGACTGAAAGTTCCGATc                                      <  2:1079758/140‑1 (MQ=11)

                              tCCCCAACCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                      <  1:2569903/140‑1 (MQ=12)

                               ccccAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATcc                                     >  1:3244629/1‑140 (MQ=37)

                               ccccAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATcc                                     >  1:3563714/1‑140 (MQ=37)

                               ccccAATCGTAAAAGCAATCACTTCTCCATTGTATAAA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATcc                                     >  1:2981522/1‑140 (MQ=14)

                                cccAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCa                                    <  2:2971387/140‑1 (MQ=35)

                                cccAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCa                                    <  2:2129753/140‑1 (MQ=35)

                                cccAATCGTAAAAGCAATCACTTCTCCATTGTATAAC‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCa                                    <  1:2196234/140‑1 (MQ=2)

                                  cAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAat                                  <  2:1682707/140‑1 (MQ=34)

                                  cAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAat                                  <  2:757270/140‑1 (MQ=34)

                                  cAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAat                                  <  2:4024660/140‑1 (MQ=34)

                                  cAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAat                                  <  2:2265490/140‑1 (MQ=34)

                                  cAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAat                                  <  2:109689/140‑1 (MQ=34)

                                   aaTCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTGCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAata                                 <  2:796787/140‑1 (MQ=21)

                                   aaTCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAata                                 <  2:607528/140‑1 (MQ=34)

                                   aaTCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAata                                 <  2:3679920/140‑1 (MQ=34)

                                    aTCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCActat                                >  1:1598862/1‑140 (MQ=21)

                                    aTCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAatat                                >  1:1200442/1‑140 (MQ=34)

                                            aGCCATCACGTCTCCACTGTATACA‑TCGACTATACTGGAAAGGTACAAGTGTTTCTGTCCATAAGGCAACTATGTGAGGTCCGTTACTAGTTTTTCACGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGa                        <  1:3404295/140‑1 (MQ=11)

                                             gCAATCACTTCTCCCTTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGAc                       <  2:3603113/140‑1 (MQ=17)

                                               aaTCTCTTATCCATTGTATCCA‑TATAATATACTTGAAATGTACATTTGTTTCTGTCCATAAGGAAAATATGTGATGTCCTTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGAcaa                     <  2:1600171/140‑3 (MQ=11)

                                               aaTCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATCTGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  1:3172604/140‑1 (MQ=17)

                                               aaTCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  1:1082386/140‑1 (MQ=32)

                                               aaTCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  2:1630506/140‑1 (MQ=32)

                                               aaTCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  1:23086/140‑1 (MQ=32)

                                               aaTCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  2:3544190/140‑1 (MQ=32)

                                               aaTCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  1:2630608/140‑1 (MQ=32)

                                               aaTCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  1:2936468/140‑1 (MQ=32)

                                               aaTCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  2:1256543/140‑1 (MQ=32)

                                               aaTCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  1:890524/140‑1 (MQ=32)

                                               aaTCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  1:3526233/140‑1 (MQ=32)

                                               aaTCACTTCTCCATTGTAGACA‑ACCACTAGACTGTCAAGGTACCAAGGTTTCGGTCCATAAGGCAAATATGTGATCTCCGTTACTAATTTTTCAAGAGGGCGAACAGACGGAAAGTTCCGATCCAATATATTATCGACCa                     <  2:3033469/140‑1 (MQ=11)

                                               aaTCACTACTCCCTTGTATACA‑TCTACTAGACTGGAAAGGTACAATTGCTTCTGTCCATAAGGCCAATATGTGATGTCCGTTCCGAGTTGTTCAAGCGGGCGATCAGACTGAACGGTCCGATCCAATATATTATCGACCa                     <  2:2611492/140‑1 (MQ=11)

                                               aaTCAATTCTCCATTGTA‑ACACTCTAATATACTGTAAAGGCACAAGTGTTTCTGTCCATAAGTCAAGTATGTGATCTCCCTTACTAGTTTTTCAAGAGGGCGACCAGACTGAAAGTTCCTATGCTATATATTATCGAcga                     <  2:3247724/140‑3 (MQ=1)

                                                             tGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGc       >  1:2358543/1‑140 (MQ=32)

                                                              gTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGcc      <  2:497495/140‑1 (MQ=32)

                                                              gTATACA‑TATAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGcc      <  2:3454053/140‑1 (MQ=17)

                                                               tataCA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCACAGATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATAAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCtccag     >  1:3079233/1‑136 (MQ=255)

                                                                ataCA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCa    <  2:1478183/140‑1 (MQ=32)

                                                                 taCA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCAt   >  2:1793170/1‑140 (MQ=32)

                                                                  aCA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAGAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCAtt  <  2:5467/140‑1 (MQ=14)

ATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACA‑TCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCATT  >  NZ_CP034943/3285580‑3285785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`