BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,269	G→C	9.3%	R36G (CGT→GGT)	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,269	0	G	C	9.3%	434.2 / 3.5	217	R36G (CGT→GGT)	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (122/50); new base C (3/19); total (126/91)
Fisher's exact test for biased strand distribution p-value = 2.82e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 227 total aligned reads displayed.

TGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGATAGCGCAG  >  NZ_CP034943/3851130‑3851406

tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACg                                                                                                                                           >  1:2761462/1‑140 (MQ=255)

tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACg                                                                                                                                           >  1:2212505/1‑140 (MQ=255)

tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACg                                                                                                                                           >  1:1127539/1‑140 (MQ=255)

tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACg                                                                                                                                           >  1:660684/1‑140 (MQ=255)

tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTAGTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACg                                                                                                                                           >  1:379560/1‑140 (MQ=255)

           ttCTCTTTTCGCTAGTTTTATCACCTTTAACCCATACCATACAAAGTACTCGCACAGTACTTCTATAAATCAACCAGTCGTTATTACCTGTATAATGATTCGATCGTATG‑CAACCCCTACCCAAAA‑ACGATAACACGAct                                                                                                                                <  2:4019012/140‑1 (MQ=255)

           ttCTATTTTCTCTATTTTTATCACTTTTTAATAATAACTTTAAATGTACTCGCACAGTTATTCTATAAATCAACCAGTTTTTTTTACCTTTATCATTATTCGATCGTATG‑CAAGCCCTTCCAAAAA‑ACGAAAACACGAct                                                                                                                                <  2:1214795/140‑1 (MQ=255)

                       tatttttATAACCTTGTAACAAAAAGATACAAATTAATCGCATAGGTTTAATATAAATTAACAAGTCTTAATTACCTGTAAAATTATTCCATCTTATG‑AACGCCCTCCCCAAAA‑ACAATAAAACGACTCTGCTTCTTCaa                                                                                                                    <  2:3158876/137‑1 (MQ=255)

                       tAGTTATAACACCTTTTAAGCAAAACCTAAAAATTACTCGGACAGGGTTTCTATAAATCACCCAGTGTTTATTACATTTTAAATTTTTCTATCGCAGC‑CAAGCCCTCCCCAAAA‑ACGATAACACGACTCTGCTTCTTCaa                                                                                                                    <  2:3395889/140‑1 (MQ=255)

                              ctCACCTTGCAACCATAACCTTCAAAGTACTCGCACAGGTATTCTAAAAATTAACCAGTCTTTATTCCCTTTATCATGATTCGATGGTATG‑CAAGCCCTGCCCAAAA‑CCTAAAAAACGACTCTGCTTCTTCAAGTTCGCa                                                                                                             <  2:2310454/139‑1 (MQ=255)

                                   cTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACa                                                                                                        >  1:2637227/1‑140 (MQ=255)

                                    ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACag                                                                                                       >  1:3122768/1‑139 (MQ=255)

                                    ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAAAGACTCTGCTTCTTCAAGTTAGCAAGACAt                                                                                                       >  1:3142497/1‑140 (MQ=255)

                                    ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTACCAAAA‑ACGATAAAACGACTCTGCTTATTCAAGTTCGGAAGACAt                                                                                                       >  1:2376871/1‑140 (MQ=255)

                                    ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTGGCAAGACaa                                                                                                       >  2:3237498/1‑139 (MQ=255)

                                    ttGTAAGCATAACCTTCAAAGAACTGTGACAGGTATTCTATAAATCAACAGGTCTTTATTACCTGTATATTTTTTCGATCTTATG‑AAACCCCTTCCCAAAA‑ACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACAt                                                                                                       <  2:2164217/140‑1 (MQ=255)

                                     ttaacgaaTAACCTCAAAAGTACGCGGACTGGTCTTTTATAAATTACCCAGTCTTTATTACCTTTAGCTTGATGCGTTCGTATG‑AAAGCCCTCCCAAAAA‑CCGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATa                                                                                                      <  2:3394960/133‑1 (MQ=255)

                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCCTGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                              >  1:1323387/1‑140 (MQ=255)

                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                              >  1:2235610/1‑140 (MQ=255)

                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCAAAAA‑ACGATAAAACGACTCTGCTTCTTAAAGTTCGCAAGACATAACAACttt                                                                                              >  2:1882222/1‑139 (MQ=255)

                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACTGTATCATGATTCGATAGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAAATAATCTGCTTATACAAGTTCTCAAGACATAAATACttt                                                                                              >  2:2971966/1‑139 (MQ=255)

                                                ccTTCAAAGTACTCGTACTGTTCTTCTATAACTGAACCAGTCGTTATTACCTGTATCATTCTTCGAGCGTATG‑CACGCCCTTCCAAAAA‑ACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                           <  2:3212947/140‑1 (MQ=255)

                                                ccTCCAACGAACTGGCACAGGTCTTCTATAACTGACCCCGTCTTTATTACCTGTTTCATGATTCGCTCGTAGC‑CAACCCCGCCCCAAAA‑ACCAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                           <  2:3038203/140‑1 (MQ=255)

                                                ccTACAAATTACTCGGACTGGTCTTATATAAATGAACCTGTCTTTATTACCTGAATCATGATGCGATCGTCTG‑CACGCCCTGCCAAAAA‑CCGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                           <  2:1397068/140‑1 (MQ=255)

                                                ccTACAAAGTAGTCGTAGAGGTATACAATAAATGAACCCTTCGTTATTCCCTTTATCATTATCCTAGCGTATC‑CACCCCCTGCCAAAAA‑ACTAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                           <  1:3720692/140‑1 (MQ=255)

                                                ccTACAAAGTACGCGCACTGTTGTTATAAAAATCACCCCGTCTTTTTTACCTGTATATTGTTTCGAGCGTATG‑CACGCCCTACCAAAAA‑ACCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                           <  1:3744126/140‑1 (MQ=255)

                                                cataaaaaCTACTCGCACAGGTATTATAAAAATGAACCTGTCGTTATTACCTTTATAATGATTCGATCGTAGG‑CAAGCCCTTCCAAAAA‑ACGTTAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                           <  2:148622/135‑1 (MQ=255)

                                                 cTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatga                                                                                          >  1:2158354/1‑140 (MQ=255)

                                                    cAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAAATTGATGAGGc                                                                                       >  1:1025924/1‑140 (MQ=255)

                                                     aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCt                                                                                      >  1:3554881/1‑140 (MQ=255)

                                                           cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagac                                                                                >  2:378925/1‑139 (MQ=255)

                                                           cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAAAACAACTTGATGAGGCTTAagag                                                                                >  1:1055423/1‑140 (MQ=255)

                                                           cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGAACGTATG‑CAATCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                                                >  1:3870855/1‑140 (MQ=255)

                                                           cTCGCACAGTTCTTATATAAATCAACCAGTCGTTATTACCTGTATCATGATTCTATCGTATG‑CAAGCCCTCCCAAAAA‑CCGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                                                <  2:1012442/140‑1 (MQ=255)

                                                               gACTGGTGTTATATAAATCAACCCGTCTTTTTTACGTTTATATTTATTCGATCGTAGG‑CAACCCCTCCCAAAAA‑ACTAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                                                            <  2:44359/140‑1 (MQ=255)

                                                                 cAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTAGATGAGGCTTAAGAGCGATCa                                                                          >  2:2280621/1‑140 (MQ=255)

                                                                 cAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTGCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCa                                                                          >  2:2644114/1‑140 (MQ=255)

                                                                             aaaTCAACCAGTCTTTTTTACGTTTATTTTGTTTCGTTC‑TGTGCCAAGCCATCCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGc                                                              <  1:2314866/140‑1 (MQ=255)

                                                                              aaTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAATTTCGCAAGACAAAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGcc                                                             >  1:889020/1‑140 (MQ=255)

                                                                              aaTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGcc                                                             >  1:3762492/1‑140 (MQ=255)

                                                                              aaTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGcc                                                             >  1:2088429/1‑140 (MQ=255)

                                                                              aaTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAAATTGATGAGGCTTAAGAGCGATCATGTCTAAATTGcc                                                             >  1:1070370/1‑140 (MQ=255)

                                                                              aaTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTAGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTATAAATTGcc                                                             >  2:2859437/1‑140 (MQ=255)

                                                                               aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAAAACAACTTGATGATGCTTAAGAGCGATCATGTCTAAATTGccc                                                            >  1:2795670/1‑139 (MQ=255)

                                                                                  accctgTCTTTATTACCTGTATATTTATTCGATCGTAGC‑CACGCCCTCCCAAAAA‑ACGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                                         <  1:1169454/135‑1 (MQ=255)

                                                                                  acccAGTCTTTTTTACCTGTATCATTATTCTACCGTAGG‑CACCCCATACCCAAAA‑ACCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                                         <  2:3466975/138‑1 (MQ=255)

                                                                                  aacctttCGTTATTACATGTATCATGTTTCCACCCTCTC‑CAAGCCATCCCCAAAA‑ACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGTCTTAAGAGCGATCATGTCTAAATTGCCGATa                                                         <  2:3319883/134‑1 (MQ=255)

                                                                                  aaCCAGTCGTTATTACCTGTATCATGATTCGATCTTATT‑CAAGACCTTCCCAAAT‑ACCATAAAAAGACTATGCTTATTCAATTTCGCAAATCATATAAAATTTATTAGTATTAATAGCGATAATGTCTAAAtttaagata                                                         >  2:3339600/1‑133 (MQ=255)

                                                                                  aaCCAGTAGTTTTTACCTTTATAATTATTCTATCTTATC‑AACGCCCGTCCCAAAA‑ACCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                                         <  2:3135261/140‑1 (MQ=255)

                                                                                   aCCAGTCGTTATTACCTGTATCATTATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACCACTTGATGAGGCTTAAGTGCGTTCATGTCTAAATTGCCGATAc                                                        >  1:3301904/1‑140 (MQ=255)

                                                                                   aCCAGTCGTTATTACCTGTATCATGATTCGAGCGTAGG‑CAAGCCCTTCCCAAAA‑ACGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATAc                                                        <  2:1468309/140‑1 (MQ=255)

                                                                                   aCCAGTCGTTATTACCTGTATAATGATTCGATCGGATG‑CAAGCCCTTCCCAAAA‑ACGATAAATCGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATAATGGGTAAATTGCCGATAc                                                        >  1:525645/1‑140 (MQ=255)

                                                                                    cctgTCTTTATTACCTGTATATTGTTTCTATCGTATG‑AAAGCCCTTCCCAAAA‑ACCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                                                       <  2:82850/137‑1 (MQ=255)

                                                                                    ccAGTGTTTTTTACCTGTACCATGTTTCGATCTTAGG‑AAAGCCCTCCCAAAAA‑ACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                                                       <  2:110682/140‑1 (MQ=255)

                                                                                    ccAGTCTTTATTACTTTTTTCTTTATTCTCTCGTCTC‑CACGCCCTCCCAAAAA‑ACCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                                                       <  2:1055423/140‑1 (MQ=255)

                                                                                    ccAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATTAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                                                       >  2:1714891/1‑140 (MQ=255)

                                                                                    ccAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                                                       >  2:3080389/1‑140 (MQ=255)

                                                                                    ccAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                                                       >  2:2417018/1‑140 (MQ=255)

                                                                                    ccAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                                                       >  2:499010/1‑140 (MQ=255)

                                                                                    ccAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCGTCCCAAAA‑ACGATAAAACGACTCTGCTTATTCAAGTTCGCAAGACATAACAACATTATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                                                       >  1:1987928/1‑140 (MQ=255)

                                                                                    ccAGTCGTTATTACCTGTATCATGATTAGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTGGCAAGACATAACAAATTGATGAGGCTTAAGATCGATCATGTCTAAATTTCCGATACt                                                       >  1:3466975/1‑140 (MQ=255)

                                                                                      aGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGTGCGATCATGTCTAAATTGCCGATACTGc                                                     >  2:1353313/1‑140 (MQ=255)

                                                                                                  cTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTa                                         >  2:69987/1‑140 (MQ=255)

                                                                                                  cTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTa                                         >  2:3040009/1‑140 (MQ=255)

                                                                                                     tATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGATGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt                                      >  1:575792/1‑140 (MQ=255)

                                                                                                     tATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt                                      >  1:928086/1‑140 (MQ=255)

                                                                                                     tATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt                                      >  1:1912484/1‑140 (MQ=255)

                                                                                                     tATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt                                      >  1:2881628/1‑140 (MQ=255)

                                                                                                     tATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCGTATAGCGGTTAttt                                      >  1:676698/1‑140 (MQ=255)

                                                                                                      aTCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTCAATTGCCGATACTGCTTATAGCGGTTATTTc                                     >  1:780739/1‑140 (MQ=255)

                                                                                                      aTCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTc                                     >  1:1344342/1‑140 (MQ=255)

                                                                                                            aTTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                               >  2:277447/1‑140 (MQ=255)

                                                                                                            aTTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                               >  1:3893359/1‑140 (MQ=255)

                                                                                                            aTTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                               >  2:223947/1‑140 (MQ=255)

                                                                                                            aTTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                               >  1:3260720/1‑140 (MQ=255)

                                                                                                            aTTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACATGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                               >  2:2226166/1‑140 (MQ=255)

                                                                                                               cgatgttATG‑AAAGACATTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTTTTTCCATAAAATc                            <  2:3877171/134‑1 (MQ=255)

                                                                                                               cGATCTTTTG‑CAAGCCCTTCCAAAAA‑ACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATc                            <  2:2481359/140‑1 (MQ=255)

                                                                                                               cGATCGTATG‑CAAGCCCTTCCCAAAA‑ACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATc                            <  2:710773/140‑1 (MQ=255)

                                                                                                               cGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATc                            >  2:3822077/1‑140 (MQ=255)

                                                                                                               cGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATc                            >  2:3738697/1‑140 (MQ=255)

                                                                                                               cGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATc                            >  1:2507690/1‑140 (MQ=255)

                                                                                                                 aTCGTATG‑AAAGCCTTTCCCAAAA‑ACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCtt                          <  2:2738193/140‑1 (MQ=255)

                                                                                                                  ttgTATG‑AAAGCCGTTCACAAAA‑AGGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTc                         <  2:2663462/138‑1 (MQ=255)

                                                                                                                       tG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt                    <  2:395703/140‑1 (MQ=255)

                                                                                                                        ttcAAGCCCTCCCAAAAA‑ACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt                    <  2:1906784/138‑1 (MQ=255)

                                                                                                                        tgaaaGCCCTTCCAAAAA‑ACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt                    <  2:2608934/137‑1 (MQ=255)

                                                                                                                             gCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg               >  1:530031/1‑140 (MQ=255)

                                                                                                                             ccccTACCCAACA‑ACCAAAACACGACTCTGATTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg               <  1:3093380/139‑1 (MQ=255)

                                                                                                                                  tcaaaaaa‑aCTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGa          <  1:1588978/136‑1 (MQ=255)

                                                                                                                                  tCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGa          >  1:956343/1‑140 (MQ=255)

                                                                                                                                  tCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGa          >  2:1759295/1‑140 (MQ=255)

                                                                                                                                  tCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGa          >  1:2692661/1‑140 (MQ=255)

                                                                                                                                  tCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGa          >  1:3417440/1‑140 (MQ=255)

                                                                                                                                  tCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGa          <  2:2881628/140‑1 (MQ=255)

                                                                                                                                  cccaaaaa‑aCTAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGa          <  1:639982/136‑1 (MQ=255)

                                                                                                                                   cccAAAACACGACACCACGACTCTGATTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCCGTTGGa          <  1:3028924/140‑1 (MQ=255)

                                                                                                                                       aaa‑cCGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGATagcg     <  2:3175019/140‑1 (MQ=255)

                                                                                                                                         a‑aCGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGATagcgca   >  2:2203043/1‑140 (MQ=255)

                                                                                                                                           aCGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGATagcgcag  <  1:2950980/140‑1 (MQ=255)

TGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATG‑CAAGCCCTTCCCAAAA‑ACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGATAGCGCAG  >  NZ_CP034943/3851130‑3851406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`