BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	4,000,117	G→T	7.5%	L121F (TTG→TTT)	EO946_RS20555 →	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	4,000,117	0	G	T	7.5%	978.1 / 21.3	345	L121F (TTG→TTT)	EO946_RS20555	MFS transporter
Reads supporting (aligned to +/- strand): ref base G (76/231); new base T (34/3); total (111/234)
Fisher's exact test for biased strand distribution p-value = 8.91e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 348 total aligned reads displayed.

CGGAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACAAATGGGAGGCGGATGGAAGC  >  NZ_CP034943/3999979‑4000256

cGGAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGc                                                                                                                                            <  1:1991011/140‑1 (MQ=255)

cGGAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAAAGGCACTCAAACGGTTGGGGAATT‑CGATCGGGAACCTTATTTTgg                                                                                                                                            >  1:1699172/1‑139 (MQ=255)

 ggAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGcc                                                                                                                                           <  1:3124119/140‑1 (MQ=255)

 ggAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGcc                                                                                                                                           <  1:1223373/140‑1 (MQ=255)

 ggAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGcc                                                                                                                                           <  1:2823386/140‑1 (MQ=255)

 ggAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGcc                                                                                                                                           <  2:3595341/140‑1 (MQ=255)

     aTGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGGTTTATTTTTGGGAAAGGCACTC‑ATAGGAGTCGGAAATTCGATCGGAAACGTTCTTTTGCCAAGc                                                                                                                                       >  2:3177435/1‑140 (MQ=255)

     aTGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGc                                                                                                                                       <  2:1234824/140‑1 (MQ=255)

        aGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCCCGTTATTTTTGGGAACGGCACTC‑AACGGAGTGGGAATTGCGTGCGGTAACGTTCTTTTGACAAGCtaa                                                                                                                                    >  2:3294843/1‑138 (MQ=255)

          cGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAAt                                                                                                                                  <  1:746027/140‑1 (MQ=255)

          cGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAAt                                                                                                                                  <  2:2881168/140‑1 (MQ=255)

          cGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAAt                                                                                                                                  <  2:715008/140‑1 (MQ=255)

            aaCCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGTCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCa                                                                                                                                <  1:1889641/140‑1 (MQ=255)

            aaCCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCa                                                                                                                                <  1:3070727/140‑1 (MQ=255)

            aaCCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCa                                                                                                                                <  2:2301059/140‑1 (MQ=255)

            aaCCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCa                                                                                                                                <  1:3821758/140‑1 (MQ=255)

            aaCCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGATTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCa                                                                                                                                <  1:2493444/140‑1 (MQ=255)

            aaCCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCCGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCa                                                                                                                                <  1:2447156/140‑1 (MQ=255)

             aCCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCaa                                                                                                                               >  2:2190476/1‑140 (MQ=255)

              ccTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCaaa                                                                                                                              <  2:100071/140‑1 (MQ=255)

              ccTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCaaa                                                                                                                              <  1:3855570/140‑1 (MQ=255)

                  aTGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTGGTTCTGCCGGCTATACTGCCGCGTTTTTTTTTGGGACCGCCCGC‑CACGGAGTCGGCATTTCGGTCGGAAACCGTCTTTTTGCAAGCTTTATCaaaaaaa                                                                                                                          >  1:521004/1‑136 (MQ=255)

                       ttGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTAGGAACGGCACTC‑ATCGGAGTCGGAAATGCGATCGGAAACCTTCTTTTTCCAAGCATAATCAAAAATAAAAAc                                                                                                                     >  2:3854406/1‑140 (MQ=255)

                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTTTTTTTTGGAACCGCACAA‑ATCGGAGTCGGAAATGCGATCGGAAAACGTGTTTTTCCAACCTTAATCAAACAaaaaaaaac                                                                                                                   >  1:2080222/1‑135 (MQ=255)

                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATAccc                                                                                                                   <  1:2612619/140‑1 (MQ=255)

                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑AACGGAGTCGGAATTTCGATCGGCAACGTTGTTTTTCCAAGCTTAATaaaaaaaaaaaaacc                                                                                                                   >  1:2990745/1‑135 (MQ=255)

                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTAGGAACGGCACTC‑ATCGGAGTCGGAAATGCGATCGGAAACGTTCTTTTGCCAAGCTTAATAAAACATaaaaaacc                                                                                                                   >  2:252436/1‑135 (MQ=255)

                         ggTCTCCTGATCCTGTTTATCGGCGTTTTCATTCGTTCTTCCGGCTATACTGCCGCTTTATTTTTAGGAAATGCAGTC‑ATCTGAGTCGGAATTGAGATCTGATAAGTTGGTATTCCAAGATTACTCACACATAAATatac                                                                                                                   >  2:3109892/1‑137 (MQ=255)

                                tGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAACCGTTCGTTTGCCCACCTTAATAAAACATAAATAACCGGTAAAg                                                                                                            >  2:1173178/1‑140 (MQ=255)

                                 gATCCTGTTTATCGGCGTTTTGATTAGTCCTGCCGGCTATACTGCCGCGATCTTTTTCGGACCGGCACTC‑ATCGGAGTCGGAATTGCGTTCGGAAACGTTCTTTTGACAAGCTTAATCAAACATAAATACCCTGAAAAGt                                                                                                           <  1:1613431/140‑1 (MQ=255)

                                   tCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCa                                                                                                         <  1:3155042/140‑1 (MQ=255)

                                    ccTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTGGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTATTTTGCCAAGCTTAATCAAAAATAAATACACAGaaaattaac                                                                                                        >  2:2615115/1‑135 (MQ=255)

                                    ccTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAg                                                                                                        <  1:1539101/140‑1 (MQ=255)

                                    ccTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAg                                                                                                        <  2:667409/140‑1 (MQ=255)

                                    ccTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATAATGCCGCTTTTTTTTTCGGAAAGGCACAC‑AAACGAGTAGGAAAATCGATCGGAAAACGTATTTTTCCAAGCTTAATAAAACAAAAATAACCATAAAAGTGAg                                                                                                        >  1:1059875/1‑140 (MQ=255)

                                    ccTGTTTATCGGCGTTTTGAATAGTTCTGCCGGCTCTACTTCCGATTTATTTTTATGAACCGGCCAC‑ATCGGAGTCGGAAAAGCCATAGGAAAACTTAATTTCCCAAGCTTAAGAAAAAATAAATACACTGAAAACTCac                                                                                                        >  2:249907/1‑139 (MQ=255)

                                    ccTGTGTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGATTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAg                                                                                                        <  1:2308612/140‑1 (MQ=255)

                                       gTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTAGGGACCGCACAC‑AAAGGCGTCGGCAATGCGCAAGGAAACCTTCATTTTGCAAACATAAAAAAAAAAAAATAACCGGAAAAATCACGGa                                                                                                     >  1:2851962/1‑140 (MQ=255)

                                        tttATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGAt                                                                                                    <  2:2682638/140‑1 (MQ=255)

                                        tttATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGAt                                                                                                    <  2:532204/140‑1 (MQ=255)

                                        tttATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGAGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGAt                                                                                                    <  1:1759449/140‑1 (MQ=255)

                                              ggCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGAt                                                                                              <  1:865577/140‑1 (MQ=255)

                                              ggCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGAt                                                                                              <  1:428532/140‑1 (MQ=255)

                                               gCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATc                                                                                             <  1:2473097/140‑1 (MQ=255)

                                               gCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATc                                                                                             <  2:59395/140‑1 (MQ=255)

                                                cGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCa                                                                                            <  1:2407640/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  2:1886266/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  2:2424405/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  2:3057595/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  2:430395/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  2:2984369/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  1:696080/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  2:2759409/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  1:959890/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  1:3208079/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  1:3302529/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  1:3455763/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  1:3563826/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  1:3772117/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  1:1452439/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  1:1440323/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                                                                          <  1:586405/140‑1 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCCGAACGGCACTA‑ATCGGAGTCGGAATTGCGATAGGAAACGTTGTTTTTGCAAGCTTAATGAAACATAAATAACCTGAAAAGTCAGGGATAATGaagagc                                                                                          >  2:2944578/1‑135 (MQ=255)

                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTAGGAACCGCAAGC‑AACGGAGTCGGAAATGCGATAGGAAACCTTCTTTTTCCAAGCTTAATAAAACAAAAATAACCGGAAAATTCAGGGATAATGAtaacc                                                                                          >  2:829234/1‑136 (MQ=255)

                                                   tttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGcc                                                                                         <  1:4001388/140‑1 (MQ=255)

                                                            ttCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCt                                                                                                                                      <  1:2187181/86‑1 (MQ=255)

                                                                 ccGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCa                                                                           <  1:1338223/140‑1 (MQ=255)

                                                                  cGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCaa                                                                          <  2:1699172/140‑1 (MQ=255)

                                                                    gCTAGACTGCAGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAACCATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATg                                                                        <  2:500760/140‑1 (MQ=255)

                                                                      tataCTGCCGCTTTATTTTTCGGAACGGCACTC‑ATAGGAGTCGGAATTGCGATAGGAAACGTTCTTTTTCCAAGGTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATAAGCCTTTATAAGACATAAATGaa                                                                      >  2:1664304/1‑140 (MQ=255)

                                                                           tGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTGTTTTGCCAAGCTTAATCAAACATAAATAACCTGAAAAGTCAGGGATAATGATCACCCTGTATACGACATCAAATAaaatat                                                                 >  1:171959/1‑140 (MQ=255)

                                                                             ccGCTTTATTTTTCGGAACGGCACTC‑ATAGGAGTCGGAATTGCGATCGGAAACGTTCTATTTCCAACCTTAATCAAACATAAATAAACTGAAAAGTCAGGGATCATGATCAGCCTGTATACCACATCAATGAATATAtta                                                               >  2:204409/1‑139 (MQ=255)

                                                                              cGCTTTGTTTTTCGGAACAGCACTT‑ATCGGCGTCGGGATTGCGATTGGTAACGTACTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGCCAGGGATCATGATCAGCCTGTATATGACATCAATGAATATATTCg                                                              <  1:3017333/140‑1 (MQ=255)

                                                                              cGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCg                                                              <  1:3338591/140‑1 (MQ=255)

                                                                              cGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCg                                                              <  1:2337832/140‑1 (MQ=255)

                                                                                  ttATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCgctgc                                                          >  2:3280180/1‑140 (MQ=255)

                                                                                   tATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCgctgct                                                         <  1:2557274/140‑1 (MQ=255)

                                                                                          cGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCAt                                                  >  1:3679135/1‑140 (MQ=255)

                                                                                                ggCACTC‑ATCGGAGTCGGAAGTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAACGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATCTATTCGCTGCTTTGGCATCAGgtg                                            <  2:3977227/140‑1 (MQ=255)

                                                                                                 gCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAATCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGgtgt                                           <  2:1922308/140‑1 (MQ=255)

                                                                                                 gCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGgtgt                                           <  1:3597457/140‑1 (MQ=255)

                                                                                                 gCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGgtgt                                           <  2:1580012/140‑1 (MQ=255)

                                                                                                     tC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGc                                       <  1:1020075/140‑1 (MQ=255)

                                                                                                        aTCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGAATCAGGTGTCAGCGt                                     >  2:3424394/1‑140 (MQ=255)

                                                                                                             aGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACctc                                >  2:1381641/1‑140 (MQ=255)

                                                                                                             aGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACctc                                >  1:2196302/1‑140 (MQ=255)

                                                                                                               tCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTg                              >  2:665788/1‑140 (MQ=255)

                                                                                                                  gAATTGCTATAGGAAACTTTCTTTTGCGAAGCTTACTGAATGATAACTCGCCTGAACAGTCAGGGTTCATGATCAGGGTGTTTTCGACTTCAATGAATATATTGGCTGCTTTGGCATGTGGTGTCAGCGTACCTCTggcg                           <  2:3646230/140‑1 (MQ=255)

                                                                                                                    aTTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTggcggc                         <  1:2752792/140‑1 (MQ=255)

                                                                                                                       gCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGcaca                      >  1:2971039/1‑140 (MQ=255)

                                                                                                                             ggAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACAAATggg                >  2:2858735/1‑140 (MQ=255)

                                                                                                                                aaCGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAATTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACAAATGGGAgg             <  2:217688/140‑1 (MQ=255)

                                                                                                                                     tCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACAAATGGGAGGCGGAt        <  2:3737396/140‑1 (MQ=255)

                                                                                                                                         ttGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACAAATGGGAGGCGGATGGac    >  1:3179990/1‑139 (MQ=255)

                                                                                                                                         ttGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACAAATGGGAGGCGGATGGaa    >  1:3851290/1‑140 (MQ=255)

                                                                                                                                         ttGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACAAATGGGAGGCGGATGGaa    >  2:1510545/1‑140 (MQ=255)

                                                                                                                                         ttGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACAAATGGGAGGCGGATGGaa    >  2:3330567/1‑140 (MQ=255)

                                                                                                                                         ttGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACAAATGGGAGGCGGATGGaa    >  2:1342921/1‑140 (MQ=255)

                                                                                                                                           gCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACAAATGGGAGGCGGATGGaagc  <  2:2080222/140‑1 (MQ=255)

                                                                                                                                           gCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACAAATGGGAGGCGGATGGaagc  <  1:1173451/140‑1 (MQ=255)

CGGAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTC‑ATCGGAGTCGGAATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCATCAGGTGTCAGCGTACCTCTGGCGGCACAAATGGGAGGCGGATGGAAGC  >  NZ_CP034943/3999979‑4000256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`