BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	1,720,712	C→A	8.0%	T212T (ACC→ACA)	spoIIIE →	DNA translocase SpoIIIE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	1,720,712	0	C	A	8.0%	583.3 / 12.0	239	T212T (ACC→ACA)	spoIIIE	DNA translocase SpoIIIE
Reads supporting (aligned to +/- strand): ref base C (140/71); new base A (2/25); total (143/96)
Fisher's exact test for biased strand distribution p-value = 2.68e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 244 total aligned reads displayed.

TTGTTATGATTTTGATCGGAATGATTTTAGTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGA  >  NZ_CP034943/1720573‑1720851

                 ggAATGATTTTAGTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑aa                                                                                                                            <  1:5234931/140‑1 (MQ=255)

                  gAATGATTTTAGTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATTAAGTCATCG‑aaa                                                                                                                           >  2:1538137/1‑140 (MQ=255)

                             gTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTCTAAAAGAACACTGGTTTGCA‑TTTATTGATG‑ATATGAAAGCCGTCAAAACGACTATGAAGTCAGCG‑AAAACAACGAAAgc                                                                                                                >  1:3642411/1‑140 (MQ=255)

                             gTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGTAGTCATCG‑ACAAAAACGACAgc                                                                                                                >  1:4037385/1‑140 (MQ=255)

                             gTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAgc                                                                                                                >  2:421772/1‑140 (MQ=255)

                             gTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAgc                                                                                                                >  1:1911145/1‑140 (MQ=255)

                                 caGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCg                                                                                                            >  1:3940544/1‑140 (MQ=255)

                                  aGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGa                                                                                                           >  1:65518/1‑140 (MQ=255)

                                  aGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGa                                                                                                           >  1:4285432/1‑140 (MQ=255)

                                  aGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGa                                                                                                           >  1:2442092/1‑140 (MQ=255)

                                  aGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGa                                                                                                           >  1:1998013/1‑140 (MQ=255)

                                  aGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTAGTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGACATCG‑AAAAAAACGAAAGCGCCgc                                                                                                           >  1:1642913/1‑139 (MQ=255)

                                            ctTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGcaaaaaaca                                                                                                 >  1:4367010/1‑140 (MQ=255)

                                              tCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGcaaaaaacaaa                                                                                               >  1:4548524/1‑140 (MQ=255)

                                              tCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGcaaaaaacaaa                                                                                               >  2:2330100/1‑140 (MQ=255)

                                                     aagccgAAAAAGACGATTAGCCCGATACGCCCCTGCATAAAAGAACAATGGTTTTCA‑ATTATTGATC‑AAATGAAATCAGTCAAAACCAATATGAAGACAACG‑AAAAAAACGAAAGCGGCGATCAAAAAACAAAATACCGc                                                                                        >  2:2651084/6‑140 (MQ=255)

                                                     aCGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAATACCAATATGAAGTCATCG‑AAATAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGc                                                                                        >  1:2160455/1‑140 (MQ=255)

                                                     aCGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGc                                                                                        >  2:633752/1‑140 (MQ=255)

                                                     aCGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGc                                                                                        >  1:1431983/1‑140 (MQ=255)

                                                     aCGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGc                                                                                        >  1:3845222/1‑140 (MQ=255)

                                                       gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAATTCAACG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                      >  2:1308605/1‑140 (MQ=255)

                                                       gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGcc                                                                                       >  2:3582977/1‑140 (MQ=255)

                                                       gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACTGccc                                                                                      >  2:1047267/1‑140 (MQ=255)

                                                       gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                      >  2:2579270/1‑140 (MQ=255)

                                                       gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                      >  1:3938347/1‑140 (MQ=255)

                                                       gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                      >  2:3002075/1‑140 (MQ=255)

                                                       gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                      >  2:4374744/1‑140 (MQ=255)

                                                       gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                      >  1:185917/1‑140 (MQ=255)

                                                       gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                      >  2:1776948/1‑140 (MQ=255)

                                                       gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                      >  1:848480/1‑140 (MQ=255)

                                                       gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                      >  1:1139171/1‑140 (MQ=255)

                                                       gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                      >  1:5457918/1‑140 (MQ=255)

                                                       gCTGAAAAAGTGGATGAGCCCGATTGGCCGATTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAATCCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAAACAAAAACGGccc                                                                                      >  2:1268676/1‑140 (MQ=255)

                                                           aaaaaGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCaa                                                                                  >  1:2790255/1‑140 (MQ=255)

                                                                 tGGATGAGCCCTATTGGCCGCATTATAAAAGAACAATGGTTTGAA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACa                                                                            <  2:3292170/140‑1 (MQ=255)

                                                                       tgCCCGATTGGCCGATTTATAAAAGAACATTGGTTTTCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATTAAGTCATCT‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAAt                                                                      <  2:1188334/139‑1 (MQ=255)

                                                                         cccGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGTCCCGCAAAAAACAGCAAATgg                                                                    >  1:3258620/1‑140 (MQ=255)

                                                                         cccGATTGGCCGATTTATAAAATAACAATGGTTTGCA‑TTTATTGATT‑ATATGAAATCCTTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATgg                                                                    <  2:5727639/140‑1 (MQ=255)

                                                                             aTTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAAcc                                                                >  2:890078/1‑140 (MQ=255)

                                                                              ttGGCCGCTTTATAAAAGAACATTGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCg                                                               <  2:4968272/140‑1 (MQ=255)

                                                                              ttGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCg                                                               <  2:75755/140‑1 (MQ=255)

                                                                                ggCCGCTTTATAAAAGAACACTGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGa                                                             <  2:1559206/140‑1 (MQ=255)

                                                                                   ctatttAAAAAACAAAATTGTTTTGAA‑TTTATTGATT‑ATATGAACTCCTACAAAACCAATATGAAGACATCT‑AAAAAAACCAACGCGCCGACCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                          <  1:2612786/137‑1 (MQ=255)

                                                                                   cgttttAAAAAACAACAA‑GGTTTGGATTTTTTTTTTT‑ATATCAAACCCGTAAAAACAAATATGAAGTAATCT‑AAAAAAACCAACGCCCCGACAAAAAAACAAAACACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                          <  1:1717009/137‑1 (MQ=255)

                                                                                   cgagttAGAACAGAACAATCTTTTGCA‑TTTATTGATG‑ATATGAAAGCCGTCAAAACCAATAGGAAGTCACCT‑AAAAAAACGAAAGCGCCGAGCAAAACACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                          <  2:2538462/136‑1 (MQ=255)

                                                                                   cGCTTTATAAAATAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                          <  2:5556470/140‑1 (MQ=255)

                                                                                   cGCTTTATAAAAGAACAATGGTTTGCA‑TTTATGGATG‑ATATGAAAGCCGACAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                          <  2:3979848/140‑1 (MQ=255)

                                                                                    gCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAAt                                                                      >  1:1210186/1‑127 (MQ=255)

                                                                                     cTTTATAAAAGAACATTGGTTTGCA‑TTTATTGATG‑ATATGAAATCCTTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCtt                                                        <  2:2222902/140‑1 (MQ=255)

                                                                                      tttATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCAGTCAAAACCAATATGAAGTCATCG‑AAAAAAACTAAAGCGCCGAGAAAAAAACAAAAAACGGCCCGCAAAAAACAGAAAATGGAACCGGATCCtta                                                       >  2:1889137/1‑139 (MQ=255)

                                                                                        tataAAAGAACATTGGTTTGAA‑TTTATTGATC‑ATATTAAATCCTTAAAAACAAATATGAAGTCATCG‑AAAAAAACTAATGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTctg                                                     <  2:1849321/140‑1 (MQ=255)

                                                                                        tataAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTctg                                                     <  2:3940495/140‑1 (MQ=255)

                                                                                        tataAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACAGGAGCCTTctg                                                     >  2:4023324/1‑140 (MQ=255)

                                                                                        taaaaaaCAAAAAGGGTTTACT‑TTTTTTTATA‑ATATTAAACCCTAAAAAACAAATATGACCCAAACA‑AAAAAAACCAACGCGCCGAGAAAAAAACAAAACACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTctg                                                     <  1:1538137/137‑1 (MQ=255)

                                                                                               caaacaTGTTTTGAA‑TTTATTGATG‑ATATTAAACCCGAAAAAACCAATATGAAGACATCT‑AAAAAAACCAACCCGCCGAGAAAAAAACAAAACACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAaga                                              <  1:3254250/135‑1 (MQ=255)

                                                                                                aaCAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAagag                                             >  1:5675436/1‑140 (MQ=255)

                                                                                                 acacgggTTTGCA‑TTTATTGAGGTTTATCATA‑CCGAAAAACCCAATTTCAAGCAAGCT‑AAAAAAACGAACCCGCCGAGCAAAAAACAAAACACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAgg                                            <  1:3709854/135‑1 (MQ=255)

                                                                                                 acacTGGTTTGCA‑TTTATTTATG‑ATATGAAATCCGTCAAAACAAATATCAAGTCATCG‑AAAAAAACTAACGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAgg                                            <  2:65518/140‑1 (MQ=255)

                                                                                                 aCAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAACACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAgg                                            <  2:821650/140‑1 (MQ=255)

                                                                                                  cAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAggg                                           >  2:2607218/1‑140 (MQ=255)

                                                                                                  cAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAggg                                           <  2:377590/140‑1 (MQ=255)

                                                                                                    aTGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAggggg                                         >  2:4463066/1‑140 (MQ=255)

                                                                                                     tgttttGAA‑TTTATTTATT‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAACGCGCCGAGAAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGa                                        <  2:3230371/137‑1 (MQ=255)

                                                                                                          tGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTgg                                   >  2:3888461/1‑140 (MQ=255)

                                                                                                          tGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTgg                                   >  1:3816117/1‑140 (MQ=255)

                                                                                                          tGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTgg                                   >  1:2973717/1‑140 (MQ=255)

                                                                                                          tGCA‑GTTATTGTTG‑ATATGAACTCCGTCAAATCCAATATGAAGTCATCG‑AAACACACGAAAGCGCCGCGCAAAACACAAAAAACGGCACGCACAAAACAGCAAAGGGAACCGGAGCCTGCTGCTGACGAGGGGGATTTgg                                   >  2:3607354/1‑140 (MQ=255)

                                                                                                           gaa‑TTTATTGATG‑ATATCAAATCCTAAAAAACAAATATGAAGTCATCG‑AAAAAAACGAACGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGa                                  <  1:5584026/138‑1 (MQ=255)

                                                                                                              tttttttGTTT‑ATATTAAATCCTTAAAAACCAATATTACTTCACCT‑AAAAAAACGAACGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGaaa                                <  1:1064462/139‑1 (MQ=255)

                                                                                                                ttatttatT‑ATATGAAATCCGTCAAAACCAATATGAAGACATCT‑AAAAAAACGACACCGCCTAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACg                              <  2:4377743/140‑1 (MQ=255)

                                                                                                                ttATTGATG‑ATATGCACTCCGTCAAAACCAATATGATGTCAGCT‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACg                              <  2:2188410/140‑1 (MQ=255)

                                                                                                                  aTTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGt                            >  2:1707769/1‑140 (MQ=255)

                                                                                                                  aTTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGt                            >  2:5229042/1‑140 (MQ=255)

                                                                                                                  aTTGATG‑ATATGAAATCCGTCAAAACCAATAAGAATTCATCT‑AAAAAAACGAAATCGACCAGAAAAAAACAAAAAACCGCCCGCAAAAAAAAGCAAATGGAACCGGATCCTTGTGAAGAAGAGGGTCATTTGGAAACGGt                            >  2:1453747/1‑140 (MQ=255)

                                                                                                                    tgatg‑aTATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAACGCGCCGAGCAAAAAACAAAACACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTAt                          <  2:2017331/140‑1 (MQ=255)

                                                                                                                    tgatg‑aTATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTAt                          <  2:459134/140‑1 (MQ=255)

                                                                                                                    tgatg‑aTATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTAt                          <  2:192764/140‑1 (MQ=255)

                                                                                                                    tgatg‑aTATGAAATCCGTCAAAACCAATAGGAAGTCAGCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTAt                          <  1:1378721/140‑1 (MQ=255)

                                                                                                                    tgatg‑aTATGAAATCCGTCAAAACAAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTAt                          <  2:2973322/140‑1 (MQ=255)

                                                                                                                        g‑atATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGGGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAACCGGTATCGcc                      >  1:889848/1‑140 (MQ=255)

                                                                                                                          atatGAAATCCGTCAAAACCAATTTGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCct                     <  2:3830860/140‑1 (MQ=255)

                                                                                                                          atatGAAAGCCGACAAAACCAATATGAAGACATCG‑AAAAAAACCAACCCGCCGAGAAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCct                     <  1:4725711/140‑1 (MQ=255)

                                                                                                                          atatCAAATCCGAAAAAACAAATATCACGAAATCC‑AAAAAAACCAACCCCCCCACAAAAAAAAAACACACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCct                     <  1:1887436/140‑1 (MQ=255)

                                                                                                                          atagGAAATCCGTCACACCCAATATGAATTCATCG‑AAAAAAACGAAAGCGCCGCTCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTATGCTGAAGAGGGGGATTTGGAAACGGTATCGCct                     <  2:1249764/140‑1 (MQ=255)

                                                                                                                          acaccaaaTCCGACAAAACAAATCTGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCct                     <  2:4342994/135‑1 (MQ=255)

                                                                                                                            atGAAATCCTAAAAAACAAATATGAAGACATCG‑AAAAAAACCAACGCCCCGAGAAAAAAACAAAACACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCctct                   <  1:3070045/140‑1 (MQ=255)

                                                                                                                            atGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCctct                   >  1:1773806/1‑140 (MQ=255)

                                                                                                                            atGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCctct                   >  1:4657206/1‑140 (MQ=255)

                                                                                                                               aaaTCCTTCAAAACCAATATGAAGTCATCT‑AAAAAAACGAAACCGCCGAGCAAAAAAAAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTat                <  2:4957417/140‑1 (MQ=255)

                                                                                                                               aaaTCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAACGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTat                <  2:132763/140‑1 (MQ=255)

                                                                                                                               aaaTCCGTCAAAACAAATATGAAGTCATCG‑AAAAAAACCAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTat                <  2:1079443/140‑1 (MQ=255)

                                                                                                                                   ccGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTattcat            >  2:2920647/1‑140 (MQ=255)

                                                                                                                                    cGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGc                       <  2:5083228/128‑1 (MQ=255)

                                                                                                                                    cGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGc                       >  1:5083228/1‑128 (MQ=255)

                                                                                                                                      tCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTAttcattca         >  2:2684347/1‑140 (MQ=255)

                                                                                                                                      tCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATGTGGAAACGCTATCGCCTCTTattcattcc         >  2:4504880/1‑139 (MQ=255)

                                                                                                                                       cAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCag        <  2:186313/140‑1 (MQ=255)

                                                                                                                                       cAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCag        <  2:5457918/140‑1 (MQ=255)

                                                                                                                                             cAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGa  <  2:961070/140‑1 (MQ=255)

                                                                                                                                             cAATACGACGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGa  <  2:3683673/140‑1 (MQ=255)

TTGTTATGATTTTGATCGGAATGATTTTAGTCACAGGGCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGGTTTGCA‑TTTATTGATG‑ATATGAAATCCGTCAAAACCAATATGAAGTCATCG‑AAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGA  >  NZ_CP034943/1720573‑1720851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`