BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,234	C→A	5.3%	M47I (ATG→ATT)	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,234	0	C	A	5.3%	849.2 / 2.9	354	M47I (ATG→ATT)	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (240/79); new base A (8/25); total (249/105)
Fisher's exact test for biased strand distribution p-value = 9.81e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 356 total aligned reads displayed.

CTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCC  >  NZ_CP034943/3851095‑3851372

      tGTACCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                       <  2:3327414/140‑1 (MQ=255)

        tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                     >  1:5283667/1‑140 (MQ=255)

        tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                     >  1:5465206/1‑140 (MQ=255)

        tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCg                                                                                                                                     >  2:4874871/1‑140 (MQ=255)

          ccAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGAt                                                                                                                                   <  2:5465206/140‑1 (MQ=255)

          ccAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGAt                                                                                                                                   <  1:5716174/140‑1 (MQ=255)

          ccAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAGTCGAt                                                                                                                                   >  2:23187/1‑140 (MQ=255)

           cactCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTGTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                  <  2:216295/137‑1 (MQ=255)

           cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                  <  1:2419213/140‑1 (MQ=255)

            aaTCTGATATATGTTATATGGTTTGTAAATGGAATTCTCTTTTCGCTAGTTTTATCACATTGTTAGCATAACCTTCAAAGTACTCTGACAGGTATTCTATATATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                                 <  2:2462216/140‑1 (MQ=255)

                  ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                           >  1:2589209/1‑140 (MQ=255)

                  ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                           >  1:19111/1‑140 (MQ=255)

                  ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGAAAGGTCTTCTATAAATGAACCATTCGTTATTACCTGTATCATGATTCGATCGTATTCa                                                                                                                           >  2:607826/1‑140 (MQ=255)

                  cacgctGTAATATGTATTGGAAATGGAATTCTCTTTTCGCTAGGTGTATCACCTTGTAAGCATAACCTTCAAAGTACACGGACAGGTCTTCTATAAATGAACCAGTCGATATTACCTGTATAATGATTCGATCGTATGCa                                                                                                                           >  2:411335/5‑140 (MQ=255)

                      ctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGcc                                                                                                                       <  2:4383278/140‑1 (MQ=255)

                       tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                      >  1:451133/1‑140 (MQ=255)

                       tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATCGTATGCAAGccc                                                                                                                      >  2:5159583/1‑140 (MQ=255)

                            tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTccc                                                                                                                 >  1:4366690/1‑140 (MQ=255)

                            tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTccc                                                                                                                 >  1:549927/1‑140 (MQ=255)

                            tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTccc                                                                                                                 >  2:55564/1‑140 (MQ=255)

                               ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaa                                                                                                             <  2:5429029/140‑1 (MQ=255)

                                gATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTATATAAATGAACCAGTCGTTATTACCTGTATCATGATTGGATCGTTTGCAAGCCCTTCCC‑aaat                                                                                                            >  1:3463998/1‑139 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAATCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTACC‑aaaaa                                                                                                           >  2:5322550/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTAGATCGTATTCAAGCCCTTGCC‑aaaaa                                                                                                           >  2:1594107/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTGGTTGGTATGCAAGCCCTTCCC‑aaaaa                                                                                                           >  1:3622790/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAATCCCTTCCC‑aaaaa                                                                                                           >  1:978280/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                           >  2:1770787/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                           >  1:4659905/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                           >  2:3012422/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                           >  1:4015058/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                           >  1:1555220/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                           >  1:2020019/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                           >  1:3368681/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                           >  1:2538739/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                           >  1:2774789/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                           >  2:698794/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaa                                                                                                             <  2:5660952/138‑1 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGACCTTCCC‑aaaaa                                                                                                           >  2:4038272/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAACCCCTTCCC‑aaaaa                                                                                                           >  2:4576489/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                           >  2:4229937/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                           >  2:5107378/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACTGTATCATGATTCGAACGTATGCAAGCCCTTCCC‑aaaaa                                                                                                           >  2:5500617/1‑140 (MQ=255)

                                 aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCAACTTGTAAGCATAACCTTCAAAGTACTCGTACAGGTCATCTATAAAAGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGCAATCCCTTCCC‑aaaaa                                                                                                           >  1:1308902/1‑140 (MQ=255)

                                  ttGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTGCCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑CAAAAc                                                                                                          >  1:1005281/1‑140 (MQ=255)

                                   tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCAAGCCCTTCCC‑AAAAACg                                                                                                         >  1:4181096/1‑140 (MQ=255)

                                   tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCTTATTCAAGCCCTTACC‑AAAAACg                                                                                                         >  1:519304/1‑140 (MQ=255)

                                   tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑CAAAACg                                                                                                         >  1:5375098/1‑140 (MQ=255)

                                   tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACg                                                                                                         >  1:2688888/1‑140 (MQ=255)

                                   tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACg                                                                                                         >  1:4441424/1‑140 (MQ=255)

                                   tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAGTCGATCGTATGCACGCCCTTCCC‑AACAACg                                                                                                         >  1:2317659/1‑140 (MQ=255)

                                     gAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAATCCCTTCCC‑AAAAACGAt                                                                                                       >  1:2056229/1‑140 (MQ=255)

                                      aaaTGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATa                                                                                                      >  1:672726/1‑140 (MQ=255)

                                                     ttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAAAGACTCTGcttc                                                                                       >  2:1484120/1‑140 (MQ=255)

                                                     ttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCGTCCC‑AAAAACGATAAAACGACTCTGcttc                                                                                       >  2:900865/1‑140 (MQ=255)

                                                                tctCACCTTGTAATCATACCCTAAAATGTACTCGGACTGGTGTTATATAAATGAACCCGTCTTTTTTACGTTTATCATTTTTCGATGGTTTCCAAGCCCTACAC‑AAAAACGATAACACGACTCTGCTTCTTCAAGTTCGc                                                                            <  2:3660102/138‑1 (MQ=255)

                                                                    aCCTTTTAAGCATAACAGTAAAAGTCCTCGGACTGTTATTTTTTAAATGACCCAGTCTTTATTACCTGTATCATTTTTCTATGTTATGCACGCACGTCCC‑AAAAACTATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGa                                                                        <  2:2664939/140‑1 (MQ=255)

                                                                       ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAt                                                                     >  1:3988635/1‑140 (MQ=255)

                                                                       ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGATATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAATTTCGCAAGACAt                                                                     >  1:407177/1‑140 (MQ=255)

                                                                          tAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAGTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATaac                                                                  >  1:2190600/1‑140 (MQ=255)

                                                                            aGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATaaaaa                                                                >  2:5260903/1‑140 (MQ=255)

                                                                                tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCAAGCCCATCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAAAAACTtg                                                            >  2:831784/1‑140 (MQ=255)

                                                                                tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                            >  2:2038161/1‑140 (MQ=255)

                                                                                tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                            >  1:5670093/1‑140 (MQ=255)

                                                                                tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                            >  2:2589154/1‑140 (MQ=255)

                                                                                tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                            >  1:4112979/1‑140 (MQ=255)

                                                                                tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAAAGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                            >  2:440400/1‑140 (MQ=255)

                                                                                 acccTTCAAAGTACTCGCACAGGTCTTATATAAATGAACCAGTCTTTATTACCTTTATCATGATTCGATCGTATCCAACCCCTCCCA‑AAACACCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtga                                                           <  2:114130/138‑1 (MQ=255)

                                                                                   ccTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatt                                                         >  2:781374/1‑139 (MQ=255)

                                                                                   ccTTCAAAGTAATCGCACTGTTCTTCTAAAAATGAACCCGTCGTTTTTACATGTATCATGTTTCGATCGTATGCAACCCCTACCC‑AAAAACGAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                         <  2:359306/140‑1 (MQ=255)

                                                                                   ccTTAAAATTACTGTCACAGGTCTTATATAAATCAACCCGTGTTTTTTACTTGTATATTTCTTCGATCGTTTGCACGCCCTCCCC‑AAAAACCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                         <  2:445895/140‑1 (MQ=255)

                                                                                   ccTACAATGTAATCGTACTGGTGTTATATCTATGACCCCGGGTTTTTTACCTGTATGTGTATTCGATCGTATCCACGCCCTTCCC‑AAAAACTCTAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                         <  2:5786455/140‑1 (MQ=255)

                                                                                   cattCAAAGTACCCCTCCCGGTATTCTATAAAACAACCAGTCTTTTTTACCTCTATCTTTATTCGTAGGTTTGCAACCCCTTCCC‑AAAAACTAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                         <  2:3926403/138‑1 (MQ=255)

                                                                                   cataaaaaTTAGTCGCACTGGTCTTCTATAAATTAACCAGTCGTTATTACCTCTATAATGTTACGATCGTTTGAACCCCCTCCCC‑AAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                         <  2:3634291/135‑1 (MQ=255)

                                                                                      tCAAAGTACTCGGAACGGTCTACTATAAATGAACCAGGCTTTATTACCTGTATAATTATTCGAGCGTATGCAAGCCCTTCCC‑AAAAACTAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAgg                                                      <  2:4181096/140‑1 (MQ=255)

                                                                                        aaaGTACTCGGACAGGTCTTCTATAAATGTCCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCACGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTGAAAGTTCGCAAGACAAAACCACGTGCGGAGGCt                                                    >  2:5704761/1‑140 (MQ=255)

                                                                                        aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCt                                                    >  1:3919426/1‑140 (MQ=255)

                                                                                              cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                              >  2:1070567/1‑140 (MQ=255)

                                                                                                  taatgttcttctATAAATCAACCTTGTGTTTTTACATTTATATTTTTTAGATCGTATTACAGCCTTGCAC‑AAAAAGTTAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                          <  2:2268122/134‑1 (MQ=255)

                                                                                                  gacggttcttctACACAGGACCCACGCTTTATACCCGGTATCACTATTCGATCGTCTCAAACCCCTCCCA‑AAAAACCATACAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGATCGAt                                          <  2:1824139/134‑1 (MQ=255)

                                                                                                  caCAGGTTTTCTAAAAATCAACACTTGTTTTTTCCCTTTATCTTGTTTCGAT‑GTTCGCCACCCCCTCCCAAAAAACCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                          <  1:114334/139‑1 (MQ=255)

                                                                                                      ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATg                                      >  1:2388466/1‑140 (MQ=255)

                                                                                                      ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCGTCCC‑AAAAACGATAAAACGACTCTGGTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATg                                      >  1:3267761/1‑140 (MQ=255)

                                                                                                            ctATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAACTTCGCACGACATAACAACTTGATGAGGCTTTAGAGCGATCATGTCGaaa                                >  1:86744/1‑140 (MQ=255)

                                                                                                             tataAATCAACCAGTTTTTATTACCTTTATCATGTTGGTATGGTATCAAAGCCATCCCA‑AAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAt                               <  1:4975227/140‑1 (MQ=255)

                                                                                                                aaaTCAACCATTCTTTATTCCCTGAATCTTTATCCGTTCGTATCCAAGCCCTACCA‑AAACACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGc                            <  1:3635738/140‑1 (MQ=255)

                                                                                                                  aTGAACCAGTCGTTATTACCTGTATCATGATTCGCTCGTATGCAAGCCCTTCCC‑AAAAACCATAAAACGCCTCTGCTTCTTGAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGTGCGATCATGTCTAAATTTCCg                          >  1:3555865/1‑140 (MQ=255)

                                                                                                                  aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAAAAAAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCg                          >  1:371553/1‑140 (MQ=255)

                                                                                                                    gAACCCTTCGTTTTTACCTGTAAATTTTTTCGATCTTTGGCAAGCCCTTCCC‑AAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGAt                        <  2:2419592/140‑1 (MQ=255)

                                                                                                                     aaCCAGGCTTTATTACCTGTATCATGTTTCGAGCGTATGCAAGCCCTTCCC‑AAAACCTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                       <  2:1420478/140‑1 (MQ=255)

                                                                                                                      aCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTGTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGTGGCTTAGGAGCGCTCAGGTCTAAATTGCCGagag                      >  2:2961451/1‑137 (MQ=255)

                                                                                                                       ccAGTCGTTATTACCTGGATCATGCTTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCAAGTGTAAATTGCCGCTACt                     >  1:592208/1‑140 (MQ=255)

                                                                                                                         aGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGc                   >  2:4671631/1‑140 (MQ=255)

                                                                                                                              ttataaCATGTATAATTATTCGACCGTATGAAAGCCGTACAA‑AAAAAATAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTtata              <  2:376233/140‑1 (MQ=255)

                                                                                                                                        tATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCCAGACATAACAACTTGATGAGGCTTAAGAGCGATCTTGTCTAAATTGCCGATACTGGTTCGAGCGGTTAttt    >  1:3153874/1‑140 (MQ=255)

                                                                                                                                        tATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt    >  1:4598462/1‑140 (MQ=255)

                                                                                                                                        tATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt    >  1:1760350/1‑140 (MQ=255)

                                                                                                                                        tATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt    >  1:1472755/1‑140 (MQ=255)

                                                                                                                                        tATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATAATCATTATAGCCGTTAttt    >  2:1204527/1‑140 (MQ=255)

                                                                                                                                        tATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATAGCCGATACTGCTTATAGCGGTTCttt    >  2:4319963/1‑140 (MQ=255)

                                                                                                                                          tCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAAAAACTTGATGAGGCTTAATAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAtttac  >  1:5120623/1‑138 (MQ=255)

CTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCC  >  NZ_CP034943/3851095‑3851372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`