BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,237	G→T	7.7%	I46I (ATC→ATA) ‡	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,237	0	G	T	7.7%	869.4 / 11.3	359	I46I (ATC→ATA) ‡	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (243/73); new base T (7/33); total (250/109)
Fisher's exact test for biased strand distribution p-value = 2.43e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 362 total aligned reads displayed.

CAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATA  >  NZ_CP034943/3851098‑3851375

   tGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                          <  2:3658083/140‑1 (MQ=255)

   tGTCACTATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                          <  2:3568777/140‑1 (MQ=255)

     tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                        >  2:114334/1‑140 (MQ=255)

     tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                        >  2:2419213/1‑140 (MQ=255)

     tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                        >  2:4165863/1‑140 (MQ=255)

     tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCg                                                                                                                                        >  2:4874871/1‑140 (MQ=255)

       ccAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGAt                                                                                                                                      >  2:5312958/1‑140 (MQ=255)

       ccAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAGTCGAt                                                                                                                                      >  2:23187/1‑140 (MQ=255)

        cactCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTGTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                     <  2:216295/137‑1 (MQ=255)

        cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                     <  2:1290913/140‑1 (MQ=255)

         aaTCTGATATATGTTATATGGTTTGTAAATGGAATTCTCTTTTCGCTAGTTTTATCACATTGTTAGCATAACCTTCAAAGTACTCTGACAGGTATTCTATATATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                                    <  2:2462216/140‑1 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCa                                                                                                                              >  1:3409141/1‑140 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                              >  2:601554/1‑140 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                              >  1:955450/1‑140 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                              >  2:5199181/1‑140 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                              >  2:2098177/1‑140 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                              >  1:2589209/1‑140 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                              >  2:697848/1‑140 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGCa                                                                                                                              >  1:2044287/1‑140 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                              >  1:19111/1‑140 (MQ=255)

                   ctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGcc                                                                                                                          <  2:4383278/140‑1 (MQ=255)

                    tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                         >  1:451133/1‑140 (MQ=255)

                         tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTccc                                                                                                                    >  1:4366690/1‑140 (MQ=255)

                         tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTacc                                                                                                                    >  2:5140863/1‑140 (MQ=255)

                              atcggAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCTTTATTACCTGTAAAATGATTCGAGCGTATTCAAGACCGTCCC‑aaaaa                                                                                                              >  1:3634291/4‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTAGATCGTATTCAAGCCCTTGCC‑aaaaa                                                                                                              >  2:1594107/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCAAGCCCTTCCC‑aaaaa                                                                                                              >  1:2318882/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                              >  1:1555220/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                              >  1:747095/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                              >  1:4103473/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                              >  1:2538739/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                              >  1:4659905/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGACCTTCCC‑aaaaa                                                                                                              >  2:4038272/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAACCCCTTCCC‑aaaaa                                                                                                              >  2:4576489/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                              >  2:4229937/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAGTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                              >  1:981795/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                              >  1:5286610/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCGTCTATAAATGAACCAGTCGTGATTAACAGTATCATGATTCGATCGTATGCAAGCCCTACCC‑aaaaa                                                                                                              >  1:5719296/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCAACTTGTAAGCATAACCTTCAAAGTACTCGTACAGGTCATCTATAAAAGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGCAATCCCTTCCC‑aaaaa                                                                                                              >  1:1308902/1‑140 (MQ=255)

                               ttGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTGCCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑CAAAAc                                                                                                             >  1:1005281/1‑140 (MQ=255)

                                tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCAAGCCCTTCCC‑AAAAACg                                                                                                            >  1:4181096/1‑140 (MQ=255)

                                tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCTTATTCAAGCCCTTACC‑AAAAACg                                                                                                            >  1:519304/1‑140 (MQ=255)

                                tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑CAAAACg                                                                                                            >  1:5375098/1‑140 (MQ=255)

                                tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACg                                                                                                            >  1:2688888/1‑140 (MQ=255)

                                tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACg                                                                                                            >  1:4441424/1‑140 (MQ=255)

                                  gAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAATCCCTTCCC‑AAAAACGAt                                                                                                          >  1:2056229/1‑140 (MQ=255)

                                   aaaTGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATa                                                                                                         >  1:2462525/1‑140 (MQ=255)

                                        gAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGAAAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGCTTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACg                                                                                                    >  2:1782976/1‑140 (MQ=255)

                                                  ttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAAAGACTCTGcttc                                                                                          >  2:1484120/1‑140 (MQ=255)

                                                  ttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCGTCCC‑AAAAACGATAAAACGACTCTGcttc                                                                                          >  2:900865/1‑140 (MQ=255)

                                                                 aCCTTTTAAGCATAACAGTAAAAGTCCTCGGACTGTTATTTTTTAAATGACCCAGTCTTTATTACCTGTATCATTTTTCTATGTTATGCACGCACGTCCC‑AAAAACTATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGa                                                                           <  2:2664939/140‑1 (MQ=255)

                                                                    ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                                    <  2:3600481/81‑1 (MQ=255)

                                                                    ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAt                                                                        >  1:3988635/1‑140 (MQ=255)

                                                                    ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGATATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAATTTCGCAAGACAt                                                                        >  1:407177/1‑140 (MQ=255)

                                                                    atGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                                    >  1:3599835/2‑81 (MQ=255)

                                                                       tAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAGTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATaac                                                                     >  1:2190600/1‑140 (MQ=255)

                                                                            aTAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGATCGCAAGACAGaacaacat                                                                >  2:5777246/1‑138 (MQ=255)

                                                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCAAGCCCATCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAAAAACTtg                                                               >  2:831784/1‑140 (MQ=255)

                                                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGTAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                               >  2:3892907/1‑140 (MQ=255)

                                                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                               >  1:5670093/1‑140 (MQ=255)

                                                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                               >  2:2589154/1‑140 (MQ=255)

                                                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAAAGATAAAACGACTCAGCTTCTTAAAGTTCGCAATACATAAAAACAtg                                                               >  1:3551610/1‑140 (MQ=255)

                                                                              aaCCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAAATtga                                                              >  2:5527441/1‑140 (MQ=255)

                                                                                ccgacAACGTACTGGCACAGGTGTTCTATAACTGAACCTGTGTTTATTACCGGTAGCTTGTTGCGATCGTTTGCATCCCGTGCCC‑AAAACCGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                            <  2:107905/136‑1 (MQ=255)

                                                                                ccTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatt                                                            >  2:781374/1‑139 (MQ=255)

                                                                                ccTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                            >  2:5652742/1‑140 (MQ=255)

                                                                                ccTTAAATGTACCCGCACTGTTTTTCTATAAAAGAACCCGTTGTTATTACCTGTTTCATTATTCCTGCGTACGCAAGCCCTGCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                            <  2:4401467/140‑1 (MQ=255)

                                                                                ccTTAAACGTACTCGCACTGTTTTTCTAAAAATCAACCCGTGTTTTTTACCTTTAACATTTTACTATCGTATCCACCCCCTCCCA‑AAAAACCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                            <  1:5527441/140‑1 (MQ=255)

                                                                                ccTTAAAATTACTGTCACAGGTCTTATATAAATCAACCCGTGTTTTTTACTTGTATATTTCTTCGATCGTTTGCACGCCCTCCCC‑AAAAACCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                            <  2:445895/140‑1 (MQ=255)

                                                                                ccTTAAAAGTACTCGGACAGGTTTTCTATAAATGAACCAGTCTTTATTACCTTTATCATGATTCGATCTTATGCACGCCCTACCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                            <  2:4103473/140‑1 (MQ=255)

                                                                                ccTACAAAGTACTCGGACCGGTCTTAAATAAATCAACCAGTCGTTTTTCCCTGTATCATGATTCGATCGTATGAAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                            <  2:3301396/140‑1 (MQ=255)

                                                                                    cAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCAGTATCATTATTAGATCCTATGCAAGACCTTACC‑AAAAACGATAAAAAGACTATGCTTCTTAAAATTAGCAACACATAACAAATTAATGAGGc                                                        >  1:3652262/1‑140 (MQ=255)

                                                                                           ctgggACTGGTCTTATATAAATCAACCCTTTTTTTTTACCTTTATAATGATTCCATGGTCTCAACCCCCTTCCA‑AAAAACTAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                 <  1:3012422/137‑1 (MQ=255)

                                                                                           cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGATGCTTAagag                                                 >  1:2757366/1‑140 (MQ=255)

                                                                                            tCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAAATTGATGAGGCTTAAGAGc                                                >  2:5671116/1‑140 (MQ=255)

                                                                                               taatgttcttctATAAATCAACCTTGTGTTTTTACATTTATATTTTTTAGATCGTATTACAGCCTTGCAC‑AAAAAGTTAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                             <  2:2268122/134‑1 (MQ=255)

                                                                                               gacggttcttctACACAGGACCCACGCTTTATACCCGGTATCACTATTCGATCGTCTCAAACCCCTCCCA‑AAAAACCATACAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGATCGAt                                             <  2:1824139/134‑1 (MQ=255)

                                                                                               caCAGGTTTTCTAAAAATCAACACTTGTTTTTTCCCTTTATCTTGTTTCGAT‑GTTCGCCACCCCCTCCCAAAAAACCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                             <  1:114334/139‑1 (MQ=255)

                                                                                                   ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATg                                         >  1:2388466/1‑140 (MQ=255)

                                                                                                   ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCGTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATg                                         >  1:4011146/1‑140 (MQ=255)

                                                                                                   ggTCTTCTATAAATGAACCAGTCGTTATTACCAGTATAAAGATTCGATCCGATGCAACCCCGTCCC‑AAAAACGATAAAACCACTCTGCTTCTTCCAGATCGCAAGACAAAACAACTTGATGAGGCTTAAGAGACATCATg                                         >  1:5477532/1‑140 (MQ=255)

                                                                                                     tcttctATAAATGAACCAGTCGTTATTAACTGTATCATGATTCGATCGTATGCAATCACATCCC‑AAAAACGATAAAACGACTCTACTTATTCAAGTTCGCACGACCTAAAAACTTTATGAGGCATAACATCGCTCATGTc                                       >  1:3240222/1‑140 (MQ=255)

                                                                                                        tatataAATCAACCCGTCTTTATTACCTCTATCATGATTCTATCTTTGGCACCCCTTTCCC‑AAAACATAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                                    <  1:1770787/138‑1 (MQ=255)

                                                                                                          tataAATCAACCAGTTTTTATTACCTTTATCATGTTGGTATGGTATCAAAGCCATCCCA‑AAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAt                                  <  1:4975227/140‑1 (MQ=255)

                                                                                                               aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGATGCTTAAGAGCGATAATGTCTAAATTGCCg                             >  2:5095384/1‑140 (MQ=255)

                                                                                                               aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCAAGTCTAAATTGCCg                             >  2:5464086/1‑140 (MQ=255)

                                                                                                                  aaCCAGGCTTTATTACCTGTATCATGTTTCGAGCGTATGCAAGCCCTTCCC‑AAAACCTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                          <  2:1420478/140‑1 (MQ=255)

                                                                                                                   aCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTGTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGTGGCTTAGGAGCGCTCAGGTCTAAATTGCCGagag                         >  2:2961451/1‑137 (MQ=255)

                                                                                                                   aCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATAc                         >  1:3892879/1‑140 (MQ=255)

                                                                                                                   aCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATAc                         >  2:5493254/1‑140 (MQ=255)

                                                                                                                   aCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAGAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATAc                         >  2:4300810/1‑140 (MQ=255)

                                                                                                                    ccAGTCGTTATTACCTGGATCATGCTTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCAAGTGTAAATTGCCGCTACt                        >  1:592208/1‑140 (MQ=255)

                                                                                                                      aGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGc                      >  2:4671631/1‑140 (MQ=255)

                                                                                                                           ttataaCATGTATAATTATTCGACCGTATGAAAGCCGTACAA‑AAAAAATAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTtata                 <  2:376233/140‑1 (MQ=255)

                                                                                                                               taCCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACATGATGAGGCTTAAGATCGATCATGTCTAAATTGCCGATACTGCTTATAGCgg             >  1:3284031/1‑140 (MQ=255)

                                                                                                                                     tATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGGTTATCGCGGTTCttt       >  2:5385202/1‑140 (MQ=255)

                                                                                                                                     tATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt       >  1:4598462/1‑140 (MQ=255)

                                                                                                                                     tATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt       >  1:1472755/1‑140 (MQ=255)

                                                                                                                                       tCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAAAAACTTGATGAGGCTTAATAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAtttac     >  1:5120623/1‑138 (MQ=255)

                                                                                                                                          tGATTCTCTCGTATGCAAGCCCTTCCC‑AAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATa  <  2:3010508/140‑1 (MQ=255)

CAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATA  >  NZ_CP034943/3851098‑3851375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`