BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,238	A→T	5.2%	I46N (ATC→AAC) ‡	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,238	0	A	T	5.2%	1085.1 / 4.4	359	I46N (ATC→AAC) ‡	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (246/83); new base T (2/25); total (250/109)
Fisher's exact test for biased strand distribution p-value = 2.63e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 371 total aligned reads displayed.

TGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAA  >  NZ_CP034943/3851101‑3851377

tGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                            <  2:3658083/140‑1 (MQ=255)

tGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                            <  1:1775004/140‑1 (MQ=255)

tGTCCCAATCTGCTCTCTCTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                            <  2:2118017/140‑1 (MQ=255)

 gTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTTTTACCTGTATCATGAGTc                                                                                                                                           >  1:5334837/1‑140 (MQ=255)

  tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAGCTGTATCATGATTCg                                                                                                                                          >  1:5011757/1‑140 (MQ=255)

  tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                          >  2:4165863/1‑140 (MQ=255)

  tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                          >  2:114334/1‑140 (MQ=255)

  tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAATGATTCg                                                                                                                                          >  1:2637864/1‑140 (MQ=255)

  tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACATGTATCATGATTCg                                                                                                                                          >  2:4488412/1‑140 (MQ=255)

  tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCg                                                                                                                                          >  2:4874871/1‑140 (MQ=255)

     cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                       <  2:1290913/140‑1 (MQ=255)

      aaTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                                      <  2:1573618/140‑1 (MQ=255)

            ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCa                                                                                                                                >  1:3409141/1‑140 (MQ=255)

            ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                                >  2:5199181/1‑140 (MQ=255)

            ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                                >  2:2404794/1‑140 (MQ=255)

            ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                                >  1:955450/1‑140 (MQ=255)

            ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                                >  1:5482001/1‑140 (MQ=255)

            ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGAAAGGTCTTCTATAAATGAACCATTCGTTATTACCTGTATCATGATTCGATCGTATTCa                                                                                                                                >  2:607826/1‑140 (MQ=255)

            cacgctGTAATATGTATTGGAAATGGAATTCTCTTTTCGCTAGGTGTATCACCTTGTAAGCATAACCTTCAAAGTACACGGACAGGTCTTCTATAAATGAACCAGTCGATATTACCTGTATAATGATTCGATCGTATGCa                                                                                                                                >  2:411335/5‑140 (MQ=255)

                 tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAATccc                                                                                                                           >  2:2675985/1‑140 (MQ=255)

                 tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                           >  2:5400554/1‑140 (MQ=255)

                 tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                           >  1:2052943/1‑140 (MQ=255)

                 tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATCGTATGCAAGccc                                                                                                                           >  2:5159583/1‑140 (MQ=255)

                 tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAAACAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                           >  2:2421623/1‑140 (MQ=255)

                      tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTccc                                                                                                                      >  2:55564/1‑140 (MQ=255)

                      tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTccc                                                                                                                      >  1:4366690/1‑140 (MQ=255)

                      tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCGTccc                                                                                                                      >  1:4389185/1‑140 (MQ=255)

                         ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaa                                                                                                                  <  1:349342/140‑1 (MQ=255)

                           atcggAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCTTTATTACCTGTAAAATGATTCGAGCGTATTCAAGACCGTCCC‑aaaaa                                                                                                                >  1:3634291/4‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAATCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTACC‑aaaaa                                                                                                                >  2:5322550/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                                >  1:747095/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                                >  1:5672605/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                                >  1:1555220/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                                >  2:698794/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                                >  2:1958861/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                                >  1:2102166/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                                >  1:1913848/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                                >  2:463166/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                                >  1:3861070/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                                >  1:4103473/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑aaa                                                                                                                  <  2:5660952/138‑1 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCGTACC‑aaaaa                                                                                                                >  1:2403691/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGACCTTCCC‑aaaaa                                                                                                                >  2:4038272/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                                >  2:4229937/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAGTCGATCGTATGCAAGCCCTTCCC‑aaaaa                                                                                                                >  1:981795/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCGTCTATAAATGAACCAGTCGTGATTAACAGTATCATGATTCGATCGTATGCAAGCCCTACCC‑aaaaa                                                                                                                >  1:5719296/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCAACTTGTAAGCATAACCTTCAAAGTACTCGTACAGGTCATCTATAAAAGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGCAATCCCTTCCC‑aaaaa                                                                                                                >  1:1308902/1‑140 (MQ=255)

                            ttGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTGCCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑CAAAAc                                                                                                               >  1:1005281/1‑140 (MQ=255)

                             tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCAAGCCCTTCCC‑AAAAACg                                                                                                              >  1:4181096/1‑140 (MQ=255)

                             tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACg                                                                                                              >  1:4652699/1‑140 (MQ=255)

                             tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACg                                                                                                              >  2:5677487/1‑140 (MQ=255)

                                     gAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGAAAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGCTTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACg                                                                                                      >  2:1782976/1‑140 (MQ=255)

                                                         ttATCACTTTGTAAGCATAACCTAAAAAGTACTGGCAAAGGTTTTATAAAAATTAACCAGTCTTTATTACTTGTATAATTTTTCGATCGTATACAACCCCTCCCC‑AAAACCGATAAAACGACTCTGCTTCTTCAAGTTCg                                                                                  <  2:2473153/140‑1 (MQ=255)

                                                           accACTTTGTACGCATAACCTTAAAAGTACTCGGACAGTTATACTATAAATCAACCCGTCGTTATTACGTGTATATTTTTCCGCTCTTTGGCACCCCCTTCCA‑AAAAACTAAAACACGACTCTGCTTCTTCAAGTTCGCa                                                                                <  2:2275718/138‑1 (MQ=255)

                                                                cTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACa                                                                           >  1:597503/1‑140 (MQ=255)

                                                                 ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                                      <  2:3600481/81‑1 (MQ=255)

                                                                 atGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                                      >  1:3599835/2‑81 (MQ=255)

                                                                          tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGTAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                 >  2:3892907/1‑140 (MQ=255)

                                                                          tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                 >  2:2589154/1‑140 (MQ=255)

                                                                          tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                 >  2:1600741/1‑140 (MQ=255)

                                                                          tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAAAGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACCTAACAACttt                                                                 >  2:824743/1‑139 (MQ=255)

                                                                          tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAAAGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                 >  2:440400/1‑140 (MQ=255)

                                                                             ccgacAACGTACTGGCACAGGTGTTCTATAACTGAACCTGTGTTTATTACCGGTAGCTTGTTGCGATCGTTTGCATCCCGTGCCC‑AAAACCGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                              <  2:107905/136‑1 (MQ=255)

                                                                             ccTTAAACGTACTCGCACTGTTTTTCTAAAAATCAACCCGTGTTTTTTACCTTTAACATTTTACTATCGTATCCACCCCCTCCCA‑AAAAACCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                              <  1:5527441/140‑1 (MQ=255)

                                                                             ccTACAATGTAATCGTACTGGTGTTATATCTATGACCCCGGGTTTTTTACCTGTATGTGTATTCGATCGTATCCACGCCCTTCCC‑AAAAACTCTAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                              <  2:5786455/140‑1 (MQ=255)

                                                                             ccTACAAAGTACTCGGACCGGTCTTAAATAAATCAACCAGTCGTTTTTCCCTGTATCATGATTCGATCGTATGAAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                              <  2:3301396/140‑1 (MQ=255)

                                                                                        ctgggACTGGTCTTATATAAATCAACCCTTTTTTTTTACCTTTATAATGATTCCATGGTCTCAACCCCCTTCCA‑AAAAACTAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                   <  1:3012422/137‑1 (MQ=255)

                                                                                        cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                   >  1:3968640/1‑140 (MQ=255)

                                                                                        cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACGTGATGATGCTTAagag                                                   >  1:5221286/1‑140 (MQ=255)

                                                                                        cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGATGCTTAagag                                                   >  1:2757366/1‑140 (MQ=255)

                                                                                            tactggTCTTATATAAATCAACCCGTCTTTATTACCTTTATCATTTTTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                               <  2:3563267/136‑1 (MQ=255)

                                                                                            caCAGGTTTTCTAAAAATCAACACTTGTTTTTTCCCTTTATCTTGTTTCGAT‑GTTCGCCACCCCCTCCCAAAAAACCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                               <  1:114334/139‑1 (MQ=255)

                                                                                                ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCGTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATg                                           >  1:4011146/1‑140 (MQ=255)

                                                                                                            aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGATGCTTAAGAGCGATAATGTCTAAATTGCCg                               >  2:5095384/1‑140 (MQ=255)

                                                                                                            aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCAAGTCTAAATTGCCg                               >  2:5464086/1‑140 (MQ=255)

                                                                                                            aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAAAAAAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCg                               >  1:371553/1‑140 (MQ=255)

                                                                                                              caaCCAGTCGTTTTTACCTTTATCATTTTTCGATCGTCTCAAAGCCCTCCAA‑AAAAACCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGAt                             <  1:1484120/139‑1 (MQ=255)

                                                                                                               acccAGTGTTTATTACCTGTATCATGATTCTAGCGGAGCCAAGCCCTACCA‑AAAAACCATAAAACGACTCTGCGTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                            <  2:549927/138‑1 (MQ=255)

                                                                                                               aaCCATTCTTTATTACCTTTATATTGATTCGATCGGATGAAAGCCCTCCCA‑AAAAACCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                            <  2:4389185/140‑1 (MQ=255)

                                                                                                               aaCCAGTCTTTATTACCTTTATAATGATTCTATCGTATGCACGCCCTACCA‑AAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                            <  1:5440147/140‑1 (MQ=255)

                                                                                                               aaCCAGGCTTTATTACCTGTATCATGTTTCGAGCGTATGCAAGCCCTTCCC‑AAAACCTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                            <  2:1420478/140‑1 (MQ=255)

                                                                                                                aCCAGTCGTTATTACCTGTATCATGTTTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTATTCCAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGGCTAAATTTCCGATAc                           >  1:4507675/1‑140 (MQ=255)

                                                                                                                aCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAACTTGCCGATaa                           >  2:2193278/1‑139 (MQ=255)

                                                                                                                aCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAACTTCGCAAGACAGAACAACTTGATGAGGCTTAAGAGCGAACATGTCTAAATTTCCGATAc                           >  1:4550832/1‑140 (MQ=255)

                                                                                                                 cctttctttattaAGTGTATCATTATTCGATCTTATCCAACCCCTTCAA‑AAAAACCAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                          <  2:4678484/133‑1 (MQ=255)

                                                                                                                 ccAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCCAGTTCGCAAGACCTAACAACTTGATGAGGCTTAAGATCGATCATGTCTAAATTGCCGATACt                          >  1:4052800/1‑140 (MQ=255)

                                                                                                                   aGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGc                        >  1:2290327/1‑140 (MQ=255)

                                                                                                                        ttataaCATGTATAATTATTCGACCGTATGAAAGCCGTACAA‑AAAAAATAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTtata                   <  2:376233/140‑1 (MQ=255)

                                                                                                                            taCCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCgg               >  1:236514/1‑140 (MQ=255)

                                                                                                                            taCCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACATGATGAGGCTTAAGATCGATCATGTCTAAATTGCCGATACTGCTTATAGCgg               >  1:3284031/1‑140 (MQ=255)

                                                                                                                                  tATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt         >  1:5147240/1‑140 (MQ=255)

                                                                                                                                  tATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt         >  2:2946019/1‑140 (MQ=255)

                                                                                                                                  tATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt         >  1:3228361/1‑140 (MQ=255)

                                                                                                                                  tATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATAGCCGATACTGCTTATAGCGGTTCttt         >  2:4319963/1‑140 (MQ=255)

                                                                                                                                   aTCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTc        >  2:880806/1‑140 (MQ=255)

                                                                                                                                   aTCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTc        >  1:2573928/1‑140 (MQ=255)

                                                                                                                                       tGATTCTCTCGTATGCAAGCCCTTCCC‑AAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATa    <  2:3010508/140‑1 (MQ=255)

                                                                                                                                       tGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATa    >  1:249438/1‑140 (MQ=255)

                                                                                                                                         aTTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa  >  1:1665911/1‑140 (MQ=255)

                                                                                                                                         aTTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa  >  1:4862990/1‑140 (MQ=255)

TGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCC‑AAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAA  >  NZ_CP034943/3851101‑3851377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`