BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	4,159,800	(G)_5→6	coding (474/771 nt)	RPPX_18505 ←	permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	4,159,795	1	.	G	92.6%	717.4 / 52.9	217	coding (479/771 nt)	RPPX_18505	permease
Reads supporting (aligned to +/- strand): ref base . (10/6); new base G (113/87); total (124/93)
Fisher's exact test for biased strand distribution p-value = 7.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 234 total aligned reads displayed.

GATGGGTGAGCGAGTGGGCGTTGACGAACGCCGAGCGGTAATTCATCAAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCA‑‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGGCCAGCCACCTGACCGGCCGTTCGCGGGTCAGCAGAGTCGCCAGGTAACC  >  CP009974/4159646‑4159940

  tgGGTGAGCGAGTGGGCGTTGACGAACGCCGAGCGGTAATTCATCAAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCA‑‑ggg                                                                                                                                                  <  1:3860549/151‑1 (MQ=255)

   gggTGAGCGAGTGGGCGTTGACGAACGCCGAGCGGTAATTCATCAAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCA‑‑ggg‑g                                                                                                                                                <  2:887821/151‑1 (MQ=255)

     gTGAGCGAGTGGGCGTTGACGAACGCCGAGCGGTAATTCATCAAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCA‑‑ggg‑ggg                                                                                                                                              >  2:2838293/1‑150 (MQ=255)

      tGAGCGAGTGGGCGTTGACGAACGCCGAGCGGTAATTCATCAAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCA‑‑ggg‑gggc                                                                                                                                             <  1:5217554/151‑3 (MQ=255)

      tGAGCGAGTGGGCGTTGACGAACGCCGAGCGGTAATTCATCAAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCCGCCAGCGCGCGGTTGTCGGCCGGGC‑CCA‑‑ggg‑gggc                                                                                                                                             >  2:4890570/1‑149 (MQ=255)

          cGAGTGGGCGTTGACGAACGCCGAGCGGTAATTCATCAAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑g                                                                                                                                         >  2:1947066/1‑151 (MQ=255)

          cGAGTGGGCGTTGACGAACGCCGAGCGGTAATTCATCAAGGTACTGTGCGGCACGTAGTCATCCTGCGCGGCCTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTGGTCGGCGGGGC‑CCAG‑GGG‑GGCGCT‑g                                                                                                                                         >  2:4534826/1‑151 (MQ=255)

            ggggggCGTTGACGAACGCCGAGCGGTAATTCATCAAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGc                                                                                                                                       <  2:1323662/148‑1 (MQ=255)

            aGTGGGCGTGGACGAACGCCGAGCGGTAATTCATCAAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGc                                                                                                                                       <  1:12334/151‑1 (MQ=255)

              tGGGCGTTGACGAACGCCGAGCGGTAATTCATCAAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGa                                                                                                                                     >  1:2793664/1‑151 (MQ=255)

               gggCGTTGACGAACGCCGAGCGGTAATTCATCAAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGAt                                                                                                                                    >  1:4066121/1‑151 (MQ=255)

                ggCGTTGACGAACGCCGAGCGGTAATTCATCAAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATa                                                                                                                                   <  1:5044978/151‑1 (MQ=255)

                 gCGTTGACGAACGCCGAGCGGTAATTCATCAAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAg                                                                                                                                  >  1:1361569/1‑151 (MQ=255)

                 gCGTTGACGAACGCCGAGCGGTAATTCATCAAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAg                                                                                                                                  >  1:801050/1‑151 (MQ=255)

                   gTTGACGCACGCCGCGCGGTAATTCATCACGGTCCTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGc                                                                                                                                <  2:2495066/151‑1 (MQ=255)

                    ttGACGAACGCCGAGCGGTAATTCATCAAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCa                                                                                                                               <  1:4906614/151‑1 (MQ=255)

                     tGACGAACGCCGAGCGGTACTTCATCAAGGTACTGTGCGGCACGTAGTCATCCTGCTAGGACGCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCAt                                                                                                                              <  1:1269813/151‑1 (MQ=255)

                           aCGCCGAGCGGTAATTCATCAAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACAGCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGc                                                                                                                        <  2:2983987/151‑1 (MQ=255)

                              ccGAGCGGTAATTCATCAAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGtt                                                                                                                     >  2:176916/1‑151 (MQ=255)

                               cGAGCGGTAATTCATCAAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTg                                                                                                                    <  1:4286709/151‑1 (MQ=255)

                                   cGGTAATTCATCAAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACgg                                                                                                                >  1:2842611/1‑151 (MQ=255)

                                   cGGTAATTCATCAAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACgg                                                                                                                <  2:4691275/151‑1 (MQ=255)

                                      aaaTTCATCAACGTACTGTGCGCCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCCGATCTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGACGCGCATGCAGACGTTGACGGTCa                                                                                                             >  2:107263/2‑151 (MQ=255)

                                       aaTTCATCAAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCaa                                                                                                            >  2:4777315/1‑151 (MQ=255)

                                          tcatcaAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAggg                                                                                                         >  1:1651667/1‑151 (MQ=255)

                                          tcatcaAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCA‑‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGt                                                                                                        >  1:2275254/1‑151 (MQ=255)

                                           catcaAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGt                                                                                                        >  2:3477917/1‑151 (MQ=255)

                                           catcaAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGt                                                                                                        >  2:1133704/1‑151 (MQ=255)

                                            atcaAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCA‑‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGttt                                                                                                      >  2:3323437/1‑151 (MQ=255)

                                             tcaAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGttt                                                                                                      <  1:2918468/151‑1 (MQ=255)

                                              caAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTg                                                                                                     >  1:3484516/1‑151 (MQ=255)

                                                 ggTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCtt                                                                                                  >  1:4684223/1‑151 (MQ=255)

                                                  gTACTGTGCGGCACGTAGTCATCCTGCTCTGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTc                                                                                                 >  1:1024467/1‑151 (MQ=255)

                                                    aCTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCgg                                                                                               >  1:419721/1‑151 (MQ=255)

                                                    aCTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCgg                                                                                               >  2:5169080/1‑151 (MQ=255)

                                                     cTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCggg                                                                                              <  1:3779963/151‑1 (MQ=255)

                                                       gtgCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCt                                                                                            <  1:4144770/151‑1 (MQ=255)

                                                       gggCGGCACGTCGTCATCCCGCTCGGCCTGCAGCAGCGATGGAGCCCCGCCGGGTTCGGCGCGCGCGGCAAGCGCGCGGTTGTCGGTCGGGCGCGG‑‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCt                                                                                            <  2:4067149/149‑1 (MQ=255)

                                                           ggCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGccc                                                                                        >  1:3543414/1‑151 (MQ=255)

                                                           ggCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGccc                                                                                        >  1:3593005/1‑151 (MQ=255)

                                                              aCGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCAt                                                                                     <  2:1237755/151‑1 (MQ=255)

                                                              aCGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCCCACGCGGCCCGCGCCCGGACGTCGGCCGGGC‑CCAG‑GGG‑GGCGCC‑GGCGACAGGCATCCCAGCGTTGACGGTCAAGGGTTTGCTTCCGGCGGccccct                                                                                     >  2:4471065/1‑149 (MQ=255)

                                                                gTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTc                                                                                   >  1:3120949/1‑151 (MQ=255)

                                                                gTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTc                                                                                   >  1:2374063/1‑151 (MQ=255)

                                                                gTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTc                                                                                   >  2:4281105/1‑151 (MQ=255)

                                                                    tCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCa                                                                               >  1:2684191/1‑151 (MQ=255)

                                                                      ctCCTGCTCGGTCTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAGGGGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCAt                                                                              <  2:749050/150‑1 (MQ=255)

                                                                      aTCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATc                                                                             >  1:4386398/1‑151 (MQ=255)

                                                                            cTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTa                                                                       >  1:3165368/1‑151 (MQ=255)

                                                                            cTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGAGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTa                                                                       >  2:3128811/1‑151 (MQ=255)

                                                                              cGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCGCGCGGCGAGCGCGCGGGCGTCGGCCGGGC‑CCA‑‑GGGTGGCGCG‑GGCGATGGGCATGCGGGCGGTGAGGGTCGAGGGTTTGTTGGGTGCCGCCCCGTTGTTCATCCCAGTAcg                                                                     >  2:781711/1‑150 (MQ=255)

                                                                               ggACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACAt                                                                    >  2:1488132/1‑151 (MQ=255)

                                                                               ggACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACAt                                                                    <  1:4662764/151‑1 (MQ=255)

                                                                                gACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACCTg                                                                   >  2:3991467/1‑151 (MQ=255)

                                                                                  cTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGc                                                                 >  1:4713926/1‑151 (MQ=255)

                                                                                  cTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGc                                                                 >  1:1528259/1‑151 (MQ=255)

                                                                                  cTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCA‑‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCa                                                                >  2:2178381/1‑151 (MQ=255)

                                                                                   tCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCa                                                                <  1:3380336/151‑1 (MQ=255)

                                                                                     caccaGCAATACATCCCCGCCGAATTCGGCGCACGCGGTCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAgg                                                              <  2:4749170/151‑1 (MQ=255)

                                                                                      accaGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGt                                                             >  1:4557805/1‑151 (MQ=255)

                                                                                      accaGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGt                                                             >  1:602016/1‑151 (MQ=255)

                                                                                       ccaGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTa                                                            >  2:2082810/1‑151 (MQ=255)

                                                                                        caGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTAc                                                           >  1:2052379/1‑151 (MQ=255)

                                                                                         aGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTAcc                                                          <  2:35102/151‑1 (MQ=255)

                                                                                         aGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTAcc                                                          <  2:117332/151‑1 (MQ=255)

                                                                                          gCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTAccc                                                         >  2:4223917/1‑151 (MQ=255)

                                                                                           cAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCg                                                        <  1:5148873/151‑1 (MQ=255)

                                                                                            aaTACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGc                                                       <  2:2276649/151‑1 (MQ=255)

                                                                                            aaTACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGc                                                       >  2:792146/1‑151 (MQ=255)

                                                                                             aTACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCa                                                      >  2:4957440/1‑151 (MQ=255)

                                                                                              tACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAg                                                     >  2:425273/1‑151 (MQ=255)

                                                                                                   ccccGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGgccc                                                >  2:3029522/1‑150 (MQ=255)

                                                                                                      cgccgAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGgccagcc                                             <  2:5224640/151‑1 (MQ=255)

                                                                                                       gccgAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGgccagcca                                            <  1:4116973/151‑1 (MQ=255)

                                                                                                       gccgAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGgccagcca                                            <  2:9727/151‑1 (MQ=255)

                                                                                                                ggCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGGCCAGCCACCTGACCgg                                   <  1:663793/151‑1 (MQ=255)

                                                                                                                ggCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGGCCAGCCACCTGACCgg                                   <  2:2236608/151‑1 (MQ=255)

                                                                                                                ggCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGGCCAGCCACCTGACCgg                                   <  2:4970791/151‑1 (MQ=255)

                                                                                                                  cgcACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGGCCAGCCACCTGACCGGcc                                 <  1:1133704/151‑1 (MQ=255)

                                                                                                                   gcACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGGCCAGCCACCTGACCGGCCg                                >  1:4745851/1‑151 (MQ=255)

                                                                                                                   gcACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGGCCAGCCACCTGACCGGCCg                                >  1:964527/1‑151 (MQ=255)

                                                                                                                    cACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCTGGCTGCCTGATTCGTCATCCTAGTACATGGTAGGTACCCGCAGGGCCAGCCACCTGACCGCCCGt                               >  1:1588086/1‑151 (MQ=255)

                                                                                                                    cACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGGCCAGCCACCTGACCGGACGt                               <  2:3587948/151‑1 (MQ=255)

                                                                                                                      cgcgGCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATCGCAGGTACCCGCCGGGCCACCCACCTGACCGCCCGTTc                             >  1:2252609/1‑151 (MQ=255)

                                                                                                                         ggCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGGCCAGCCACCTGACCGGCCGTTcgcg                          >  1:1717302/1‑151 (MQ=255)

                                                                                                                         ggCCAGCGCGCGGGTGGCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATGCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGGCCAGCCACCTGACCGGCCGTTcgcg                          <  2:4045910/151‑1 (MQ=255)

                                                                                                                          gCCAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGGCCAGCCACCTGACCGGCCGTTCGCgg                         >  1:695285/1‑151 (MQ=255)

                                                                                                                           ccAGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGGCCAGCCACCTGACCGGCCGTTCGCggg                        >  2:2457716/1‑151 (MQ=255)

                                                                                                                             aGCGCGCGGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATCGCAGGTACCCGCAGGGCCAGCCACCTGACCGGCCGCTCGCgggcc                      >  2:4389132/1‑149 (MQ=255)

                                                                                                                               cgcgcgTTTTTCTGCCGGGG‑GCA‑‑GGG‑GGGGGTGGGCGATAGGCATTCAGCCGTTGTCGGGCTGGGGTTTGCTTCGGGGTGCCCCATTCGTCATCCCAGTGCATGGCAGGTACCCGGAGGTCCAGCCACCTGGCCGGCCGTTCGCGGGTcag                    <  2:2674440/151‑1 (MQ=255)

                                                                                                                               cgcgcgGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGGCCAGCCACCTGACCGGCCGTTCGCGGGTcag                    >  1:3550267/1‑151 (MQ=255)

                                                                                                                                gcgcgGTTGTCGGCCGGGC‑CCA‑‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGGCCAGCCACCTGACCGGCCGTTCGCGGGTcagca                  >  2:1620919/1‑151 (MQ=255)

                                                                                                                                 ggcgGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATGCAGGCGTTGACGGTCAAGGGTTGGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGGCCAGCCACCTGACCGGCCGTTCGCGGGTcagca                  <  2:656464/150‑1 (MQ=255)

                                                                                                                                 cgcgGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGGCCAGCCACCTGACCGGCCGTTCGCGGGTccgca                  >  1:302659/1‑151 (MQ=255)

                                                                                                                                 cgcgGTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGGCCAGCCACCTGACCGGCCGTTCGCGGGTcagca                  <  1:3743799/151‑1 (MQ=255)

                                                                                                                                    ggTTGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGGCCAGCCACCTGACCGGCCGTTCGCGGGTCAGCagag               >  1:2213239/1‑151 (MQ=255)

                                                                                                                                      ttGTCGGCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGGCCAGCCACCTGACCGGCCGTTCGCGGGTCAGCAGAGTc             <  2:1536428/151‑1 (MQ=255)

                                                                                                                                            gCCGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGGCCAGCCACCTGACCGGCCGTTCGCGGGTCCGCCGAGCCGCCAgg       >  2:2894399/1‑151 (MQ=255)

                                                                                                                                              cGGGC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGGCCAGCCACCTGACCGGCCGTTCGCGGGTCAGCAGAGTCGCCAGGTa     <  1:2152272/151‑1 (MQ=255)

                                                                                                                                               gggC‑CCAG‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGGCCAGCCACCTGACCGGCCGTTCGCGGGTCAGCAGAGTCGCCAGGTaa    >  1:76841/1‑151 (MQ=255)

GATGGGTGAGCGAGTGGGCGTTGACGAACGCCGAGCGGTAATTCATCAAGGTACTGTGCGGCACGTAGTCATCCTGCTCGGACTCCACCAGCAATACATCCCCGCCGAATTCGGCGCACGCGGCCAGCGCGCGGTTGTCGGCCGGGC‑CCA‑‑GGG‑GGCGCT‑GGCGATAGGCATTCAGGCGTTGACGGTCAAGGGTTTGCTTCGGGCTGCCCCATTCGTCATCCCAGTACATGGCAGGTACCCGCAGGGCCAGCCACCTGACCGGCCGTTCGCGGGTCAGCAGAGTCGCCAGGTAACC  >  CP009974/4159646‑4159940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`