BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	1,040,909	A→C	11.3%	E232D (GAA→GAC)	rbn →	ribonuclease BN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	1,040,909	0	A	C	11.3%	297.2 / 3.7	167	E232D (GAA→GAC)	rbn	ribonuclease BN
Reads supporting (aligned to +/- strand): ref base A (77/65); new base C (2/22); total (79/88)
Fisher's exact test for biased strand distribution p-value = 1.54e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 167 total aligned reads displayed.

GGATGATGGAAGGCAGATTAATGGCGCAGATTACTTAGCCGTTCCAGTGCCGGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACA  >  NZ_CP022686/1040771‑1041045

ggATTATGGAAGGAAGATTAATGGCGCAGATTAGTTAGCCGTTCCTGTGCCGGGTAAAGCGCTGGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGAGGTAATGGTTCACGAAg                                                                                                                                         <  4:377059/140‑1 (MQ=255)

ggATGATGTAAGGCAGATTAATGGCGCATATTACTTAGCCGTTCCTGTGCCGGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCGGGCGATGCCGCGCTGGAGCTGGCTAATGGCGTCGACGTAATGGTTCACGAAg                                                                                                                                         <  5:566910/140‑1 (MQ=255)

ggATGATGGAAGGCAGATTAATTGCGATTTTTACTTAGCGGTTCCAGTGCCGTGTAAAGGGCTCGCTATTTTCGGCTATTCCGGCCCCTGCGTGGCCGCGCTGGTGCTGGATAAAGGGGTCGAGGTAATGGTTCACGAAg                                                                                                                                         <  2:368051/140‑1 (MQ=255)

 gatgatGGAAGGCAGATTAATGTCGCAGATTACTTAGCCGTTCCAGTGCCGGGTAAAGCGCTCGCTAATTTCGTCGATACCGTCCCATGCGATGACGCGCTGGAGATGGAGAAAGGCGTCGCCGGAATGGTTCACGCAGc                                                                                                                                        >  2:356889/1‑140 (MQ=255)

   tgatgGAAGGCAGATTAATGGCGCAGATTACTTAGCCGTTCCAGTGCCGGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGa                                                                                                                                      >  6:87921/1‑140 (MQ=255)

     ttgGAAGGAATATTAATGGCGCAGATTACTTAGCCGTTCCAGTGCCGGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGAct                                                                                                                                    <  1:684041/139‑1 (MQ=255)

     ctgGAAGGCCGATTATTGGCGCATATTCATTAGCCGTTCAAGGTCCGGTGAAAGGGCGCGCTAGTTTGGCCTATACCGCCCCCTGCGTTGCTGCGCTGTAGCTGGCTAAAGGCGTCGACTTAATGGGTCACGACGCGAct                                                                                                                                    <  2:274849/139‑1 (MQ=255)

     atgTAAGGCAGATTAATGGCGCCGATTAATTCGCCGTTCAAGTGCCGGTTAAAGCGCTCGTTATTTTCGGCGATACCGGCCGCTGCGATGCCGCGCTGTAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGAct                                                                                                                                    <  7:588216/140‑1 (MQ=255)

     atgTAAGGCAGATTAATGGCGCAGATTACTTAGCCGTTCCAGTGCCGGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCGCTGGAGCTGGATAAAGGCGTCGACGTAATGGTTCACGAAGCGAct                                                                                                                                    <  3:561188/140‑1 (MQ=255)

     atgGAGGGCAGATTAATGGCGCAGATTACTTAGCGGTTCAAGTGCCGGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGTTGCCGCGATGGAGCTGGCTAATGGCGTCGACTTAATGGTTCACGAAGCGAct                                                                                                                                    <  5:118091/140‑1 (MQ=255)

     atgGAAGGCAGATTAATGGCGCAGATTACTTAGCCGTTCCAGTGCCGGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCGCTGTAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGAct                                                                                                                                    <  8:906084/140‑1 (MQ=255)

      tgGAAGGCAGATTAATGGCGCAGATTACTTAGCCGTTCCAGTGCCGGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCgccgc                                                                                                                                   >  6:622768/1‑136 (MQ=255)

          cggctcaTTAATGGCGCAGATTACTTAGCCGTTCCAGTGCCGGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCGCGGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGACGCGACTCTGGa                                                                                                                               <  3:871943/134‑1 (MQ=255)

          cggCATATTAATGGCGCAGATTACTTAGCCGTTCCAGGGCGGGGTAAAGCGCTCGCTATTTTCGGCGAAACCGCCCCCTGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACTTAATGGTTCCCGAAGCGACTCTGGa                                                                                                                               <  3:204620/139‑1 (MQ=255)

          aGGCAGTTTAATGGCGCAGATTAATTAGCCGTTCCAGTGCCGGGTAAAGCGCTCGCTATTTGGGGCTATACCGCCCCCTGCGATGCCGCGCTGTTGCTGGCTAAAGGCGTCGCCGTATTGTTGCACGAAGCGACTCTGGa                                                                                                                               <  3:395085/140‑1 (MQ=255)

                aTTAATGGCGCAGATTACTTAGCCGTTCCAGTGCCGGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGc                                                                                                                         >  4:639537/1‑140 (MQ=255)

                aTTAATGGCGCAGATTACTTAGCCGTTCCAGTGCCGGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCGCGGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGACGCGACTCTGGATATggg                                                                                                                         >  1:811259/1‑139 (MQ=255)

                aTTAATGGCGCAGATTACTTAGCCGTTCCAGTGCCGGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGAGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGc                                                                                                                         >  4:905558/1‑140 (MQ=255)

                    agggCGCAGATTACTTAGCCGTTCCAGTGCCGGGTCAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCGCTGGAGCGGGCTAAAGGCGTCGACGTAATGGTTCACGACGCGACTCTGGATATGGCGATg                                                                                                                     <  2:919585/138‑1 (MQ=255)

                           aGATTACTTAGCCGTTCCAGTGCCGGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCGCGGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCa                                                                                                              <  2:41437/140‑1 (MQ=255)

                                  ttcgcggTTCCAGGGCGGGGAAACGCGCTCGCTATTTTCGCCGAAACCGGCCCCTGCGTTGCCGCGCGGGCGGTGGCAAATGGGGGCGACGTATTGGTCCACTAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCaa                                                                                                       <  4:326447/134‑1 (MQ=255)

                                      ccGTTCCAGTGCCGGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCGCGGGAGCTGGCTAAAGGCGGCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGt                                                                                                   <  8:311899/140‑1 (MQ=255)

                                      ccGTTCCAGGGCGGGGTAACGCGCTCGATATTTTCGGCGATACCGGCCCCGGCTCTGCCCCGCTGGAGCGGGCTAACGGCGTCTACGTAATGGTGCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGt                                                                                                   <  6:165196/140‑1 (MQ=255)

                                        gTTCCAGTGCCGGGTAACGCGCTCGCTATTTTCGGCGATCCCGGCCCCTGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACGTAAGGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTcg                                                                                                 <  4:635523/140‑1 (MQ=255)

                                         ttCCAGTGCCGGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTcgc                                                                                                >  4:800259/1‑140 (MQ=255)

                                         ttCCAGTGCCGGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTcgc                                                                                                >  3:805206/1‑140 (MQ=255)

                                         ttCCAGTGCCGGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTACCGAAGCGACTCTGGATATGGCGATGGAGGCCAACGCCAACAGgcgc                                                                                                >  5:34931/1‑140 (MQ=255)

                                         ttCCAGGGCCGGGTAAAGCGCGCGCTATTTTCGGCGATACCGGCCCCGGCGATGCCGCGCGGGAGCTGGATAACGGCGTCGACGTAATGGTGCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTcgc                                                                                                <  8:13546/140‑1 (MQ=255)

                                         gtCCTGTGCCGGGTAACGCGCTCGCTATTTTCGGCCATACCGGCCCCTGCGATGCCGCGCTGGAGCTGGCTAACGGAATCGACGAAATGGTCCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTcgc                                                                                                <  5:829749/139‑1 (MQ=255)

                                           ccAGTGCCGGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCgg                                                                                              >  6:468223/1‑140 (MQ=255)

                                            cAGTGCCGGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCGCCGATGCGGCGCTGCAGCTGGCTATCGGCGTCGACGTAATGGTGCACTAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGc                                                                                             <  7:85803/140‑1 (MQ=255)

                                            cAGTGCCGGGAAAAGCGCTCTCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCGCTGGAGCGGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGc                                                                                             <  4:128324/140‑1 (MQ=255)

                                            cAGGGCCGGGAACAGCGCTCGCTATTTCCGGCGTTACCGGCCCGTGCGAGGCCGCGCGGGAGCGGGCTAAAGGCGGCGACTTATGGGCTCACCACGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGc                                                                                             <  8:199145/140‑1 (MQ=255)

                                              gggCCGGGTAACGCGCTCGTTATTTTCGGCTATACCGGCCCCTGCGATGCCGCGCGGGAGCTGGCTAACGGGGGCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCa                                                                                           <  5:631574/138‑1 (MQ=255)

                                                    ggTAAAGCGCTCGCTATTTTCGGGGATACCGGCCCCGGCTAGGCCGCGCTGTAGCTGGCTAAAGGCGGCGACGTAAGGGTACACGACGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTc                                                                                     <  4:281727/140‑1 (MQ=255)

                                                    ggTAAAGCGCTCGCTATATTCGGCGATACCGGCCCCTGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGACTATGGATATGGCGATGGAGGCCAAAGCCAACAGTAGCGGCCATAGtta                                                                                     >  3:418817/1‑139 (MQ=255)

                                                      aaatgcgcTGGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCGCTGGAGCGGGCAAAAGGCGACTACTTAATGGTTCACGACGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCtc                                                                                   <  2:727067/136‑2 (MQ=255)

                                                      aaaaGCGCTCGCTATTTTCGGCGATCCGGCCCCCTGCTATGCCGCGCTTGAGCTGGATAAAGGCGTCTACGTAATGGTACACCAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTa                                                                                   <  7:63377/139‑1 (MQ=255)

                                                         aGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTacac                                                                                >  3:505477/1‑140 (MQ=255)

                                                           cgctcgctATTTTCGGCGATACCGGCCCCTGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGc                                                                              >  8:154225/1‑140 (MQ=255)

                                                              tcgctATTTTCGGCGATACCGGCCCCTGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAg                                                                           >  1:951517/1‑140 (MQ=255)

                                                              tcgctATTTTCGGCGATACCGGCCCCTGCGATGCAGCGCTGGAGCTGGCTAAAGTCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAg                                                                           >  8:888158/1‑140 (MQ=255)

                                                               cgctATTTTCGGCGATACCGGCCCCTGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACGGAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCACCAGTCGCGGCCAGAGTTCTACACGCCCgg                                                                          >  7:856782/1‑140 (MQ=255)

                                                                  tagtttCGGCGAAACCGGCCCCGGCGAGGCCGCGCGGGCGCGGGCTAATGGCTTCCCCGTAATGGTCCCCGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAggcgg                                                                       <  3:681685/137‑1 (MQ=255)

                                                                  tATTTTCGGCTATACCGGCCCCTGCGATGCCGCGCGGGAGCTGGCTAAAGGCGGCGACGTAATGGTTCACGACGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAggcgg                                                                       <  8:608576/140‑1 (MQ=255)

                                                                  tATTTTCGGCGATACCGGCCCCGGCGAGGCCGCGCTGTCGCGGGCTAAAGGCGGCGACGAATTGGGCCCCGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAggcgg                                                                       <  1:105291/140‑1 (MQ=255)

                                                                  tATTTTCGGCCCTACCGGCCCCTGCGATGCCTCGGGGGAGCTGGCTAACGGCGTCGCCGTATTGGTGAACGAAGCGACTCTGGATATGGCGATGGCGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAggcgg                                                                       <  5:756400/140‑1 (MQ=255)

                                                                       tCGGCGATACCGGCCCGTGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCa                                                                  <  2:932319/140‑1 (MQ=255)

                                                                       tCGGCGATACCGGCCCCTGCGATGCCGCGCTGGAGCGGGCTAAAGGCGTCGCCGTAAGGTTTCACGACGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCa                                                                  <  4:221289/140‑1 (MQ=255)

                                                                        cGGCGATACCGGCCCCTGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACCCGCCAGGCGGCGGCAc                                                                 >  8:692357/1‑140 (MQ=255)

                                                                          gCGATACCGGCCCCTGGGAGGCCGCGCGGGAGCGGGCTAAAGGCGTCGACGTAATGGGTCCCGACGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTa                                                               <  6:591815/140‑1 (MQ=255)

                                                                                cgggCCCTTGCGCTGCCGGGCGGTCGGTGGCAAAAGGCGGCGAGGTAATGGTCCTCTCCGCGACTCTGGATATGGCGTTGGAGGCCAAAGCCAACAGTCGCGGCCATTGTGCTACTCGCCAGGCGGCGGCACTAGCCCGt                                                         <  2:646932/138‑1 (MQ=255)

                                                                                ccGGCCCCTGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGt                                                         <  2:541859/140‑1 (MQ=255)

                                                                                   gCCCCTGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAg                                                      >  8:209207/1‑140 (MQ=255)

                                                                                        tGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACCCGCCATGCGGCGGCACTAGCCCGTGAGGCTgg                                                 >  3:661490/1‑140 (MQ=255)

                                                                                        tGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCGCGTGAGGCTgg                                                 >  4:881717/1‑140 (MQ=255)

                                                                                        tGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTgg                                                 >  2:831661/1‑140 (MQ=255)

                                                                                        tGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTgg                                                 >  2:526854/1‑140 (MQ=255)

                                                                                        tGCGATGCCGCGCTGGAGCTGGATAAAGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAATCCAACAGTCGAGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTgg                                                 >  7:418100/1‑140 (MQ=255)

                                                                                         gCGAGGCCGCGCTGGAGCGGGCAAATGCCGACGACGTATGGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGa                                                <  7:848231/140‑1 (MQ=255)

                                                                                               ccGGGCTGGTGGTGGCTATAGGAGGCGACGTAAGGGTTCACGACGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGt                                          <  7:274293/140‑1 (MQ=255)

                                                                                               ccGCGCTGGCGGGGGCTAAAGGCGTCGACTTAATGGTTCACGACGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGt                                          <  8:784724/140‑1 (MQ=255)

                                                                                               ccGCGCTGGAGCTGGCTAAAGGCGTCGACTTAATGGTTCACGACGCGACTCTGGATCTGGCGATGGAGGCCAAAGCCAACAGGCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGt                                          <  6:13367/140‑1 (MQ=255)

                                                                                               ccGCGCTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGATGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCCGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGt                                          >  7:477922/1‑140 (MQ=255)

                                                                                               ccGCGCGGGAGCGGCCTAACGGCGGCGCCGTAACGGTTCACTACGCGCCTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGt                                          <  6:359568/140‑1 (MQ=255)

                                                                                                  cgcTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGCGGCCAAAGCCAACAGTCGCGACCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAg                                       >  6:102731/1‑140 (MQ=255)

                                                                                                  cgcTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAg                                       >  2:536994/1‑140 (MQ=255)

                                                                                                  cgcTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAg                                       >  6:582500/1‑140 (MQ=255)

                                                                                                    ttGGAGCTGGCTAACGGCGGCGACTTAATGGTTCACGACGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCt                                     <  1:267465/139‑1 (MQ=255)

                                                                                                    cTGGCGCTGGCTAAAGGCGTCGCCGTAATGGTTCACGACGCGACGCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCt                                     <  4:241931/140‑1 (MQ=255)

                                                                                                    atgtaGCTGGCTAAAGCGGTCGACGAATTGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCt                                     <  6:758168/136‑1 (MQ=255)

                                                                                                       tcgcgggCTAAAGGCGTCGACGTAATGTTTCCCTACGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAAt                                  <  3:289064/135‑1 (MQ=255)

                                                                                                       gAGGTGGTTAAGGGCGTCGAAGTATTGGGTCCCGAAGCGACTCTGGATATGGCGATGGCGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAAt                                  <  4:332393/140‑1 (MQ=255)

                                                                                                       gAGCTGGCTAAAGGCGTCTAGTTAATGGTTCACCACGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAAt                                  <  7:174866/140‑1 (MQ=255)

                                                                                                         gcgggCTAACGGCGTCTACTAATTGGTTCACTAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAATCa                                <  3:287286/137‑1 (MQ=255)

                                                                                                         gCTGGCTAAAGGCGTCGACGTAATGTTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAATCa                                <  1:620759/140‑1 (MQ=255)

                                                                                                              cTAAAGGCGTCGACGTAATGGTGCACGAAGCGACTCTGTATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGATGGAGTCGGTAAGCTAATCATTAcc                           <  5:431233/140‑1 (MQ=255)

                                                                                                                  aGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAATCATTACACACg                       >  1:522771/1‑140 (MQ=255)

                                                                                                                       tCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAATCTTATCCCACGTCAGc                  >  2:765137/1‑140 (MQ=255)

                                                                                                                       tCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAATCATTACCCCCGTCAGc                  >  3:79855/1‑140 (MQ=255)

                                                                                                                       tCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAATCATTACCCACGTCAGc                  >  5:850408/1‑140 (MQ=255)

                                                                                                                       tCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAATCATTACCCACGTCAGc                  >  2:203380/1‑140 (MQ=255)

                                                                                                                       tCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAATCATTACCCACGTCAGc                  >  2:765134/1‑140 (MQ=255)

                                                                                                                       tCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAATCATTACCCACGTCAGc                  >  7:13176/1‑140 (MQ=255)

                                                                                                                       tCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGGGAGGCTGGAGTCGGTAAGCTAATCATTACCCACGTCAGc                  >  1:603172/1‑140 (MQ=255)

                                                                                                                       tCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAAAAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAATCATTACCCACGTCAGc                  >  1:883103/1‑140 (MQ=255)

                                                                                                                        ctaCGTAAGGGTACACGACGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAATCATTACCCACGTCAGCt                 <  3:474173/138‑1 (MQ=255)

                                                                                                                                ttgTTCACTAAGCGACTCTGGATATGGAGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAATCATTACCCACGTAAGCTCGCGCTat         <  3:365494/138‑1 (MQ=255)

                                                                                                                                ttgTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAATCATTACCCACGTCAGCTCGCGCTat         <  4:810448/138‑1 (MQ=255)

                                                                                                                                tgttccACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAATCATTACCCACGTCAGCTCGCGCTat         <  3:343008/135‑1 (MQ=255)

                                                                                                                                 ggTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAATCATTACCCACGTCAGCTCGCGCTatg        <  4:505477/140‑1 (MQ=255)

                                                                                                                                   ttCCCGAAGCGACTCTGGAGATGGCGATGGAGGCCAAAGCCAACAGGCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAATCATTACCCACGTCAGCTCGCGCTatgat      <  5:422629/140‑1 (MQ=255)

                                                                                                                                     cACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCgg                                           <  4:468923/101‑1 (MQ=255)

                                                                                                                                     cACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAATCATTACCCACGTCAGCTCGCGCTAtgatga    <  7:829013/140‑1 (MQ=255)

                                                                                                                                     cACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAATCATTACCCACGTCAGCTCGCGCTAtgatga    >  4:738169/1‑140 (MQ=255)

                                                                                                                                     cACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAATCATTACCCACGTCAGCTCGCGCTAtgatga    >  2:634044/1‑140 (MQ=255)

                                                                                                                                     cACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAATCATTACCCACGTCAGCTCGCGCTAtgatga    >  2:259250/1‑140 (MQ=255)

                                                                                                                                     cACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAATCATTACCCACGTCAGCTCGCGCTAtgatga    >  6:4818/1‑140 (MQ=255)

                                                                                                                                     cACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAAACATTACCCACGTCACCTCGCGCTAtgatga    >  3:604986/1‑140 (MQ=255)

                                                                                                                                     cACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGCCCAAAGTTCTAAACGCCAGGCGGCGGCACTATCCCGTGAGGCTGGAGTCGGTAAGCTAAACATTACCCACGACAGCACGCGCTAtgatga    >  5:624896/1‑140 (MQ=255)

GGATGATGGAAGGCAGATTAATGGCGCAGATTACTTAGCCGTTCCAGTGCCGGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCGCTGGAGCTGGCTAAAGGCGTCGACGTAATGGTTCACGAAGCGACTCTGGATATGGCGATGGAGGCCAAAGCCAACAGTCGCGGCCATAGTTCTACACGCCAGGCGGCGGCACTAGCCCGTGAGGCTGGAGTCGGTAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACA  >  NZ_CP022686/1040771‑1041045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`