Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 360,227 T→G 17.5% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP022686360,2270TG17.5% 196.7 / 16.7 152T35P (ACG→CCG) yeaRDUF1971 domain‑containing protein YeaR
Reads supporting (aligned to +/- strand):  ref base T (76/46);  new base G (25/5);  total (101/51)
Fisher's exact test for biased strand distribution p-value = 3.17e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

CTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGCAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGT  >  NZ_CP022686/360092‑360365
                                                                                                                                       |                                                                                                                                           
cTGGAGGGAACACCGCAAACTGACCCGCTTCGATAAGGATCACCTGATCAGGCGCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTTGGTAAACCCCCGGGCGCGTTCCt                                                                                                                                         <  3:984681/140‑1 (MQ=255)
     gggAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGCTCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGGTGCtttt                                                                                                                                      >  1:793103/1‑137 (MQ=255)
        aaCACCGCAAACTGCCCCGCGTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGAGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAg                                                                                                                                 >  8:152022/1‑140 (MQ=255)
               cAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGAc                                                                                                                            >  2:966035/1‑138 (MQ=255)
               cAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACAGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGGTCAGTTATAAATATTACGt                                                                                                                          >  6:139738/1‑140 (MQ=255)
                       cccGCTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCGTGCAGAACGGAAAGGCGTTGGTAAACCCCCGGGCGCGTTCCGGTATAAAGATGCCGTGAGAatat                                                                                                                  >  3:1026565/1‑140 (MQ=255)
                           cTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAATATGACGTTCGAATATTcac                                                                                                              >  3:395251/1‑138 (MQ=255)
                           cTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTACg                                                                                                              >  4:462817/1‑140 (MQ=255)
                                      aTCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATAAAGATGCCGTTAGCATATTACGGCA‑GGCGCCgt                                                                                                  >  3:852371/1‑140 (MQ=255)
                                      aTCAACTGATCAGGCTCTGCTCTGTGTTCATCATCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAgt                                                                                                  <  4:696776/140‑1 (MQ=255)
                                         aCCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGGTCCGTTATCAAGATGCCGTTCGAATATTGCGGGC‑GGCGCAgtttg                                                                                               >  6:388735/1‑140 (MQ=255)
                                          ccTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTAAAAAAGTTACGTTGGAATATTCACGCA‑GGCGCAgtttgt                                                                                              >  3:885617/1‑140 (MQ=255)
                                           cTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAgtttgtt                                                                                             <  5:969971/140‑1 (MQ=255)
                                           cTGAGCAGGCTCTGCTCTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATACCGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAgtttgtt                                                                                             <  4:512268/140‑1 (MQ=255)
                                              aTCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCATTATCAAGATGACGTTGGAATATTCCGGGA‑GGCGCAGTTTGTTTAt                                                                                          >  7:500471/1‑140 (MQ=255)
                                                cAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGCCGTTCGAATATTACGGCA‑GGCGCAGGTTGTTTAt                                                                                          >  2:696256/1‑138 (MQ=255)
                                                     tctGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGaa                                                                                   >  4:953934/1‑140 (MQ=255)
                                                     tctGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGaa                                                                                   >  6:733441/1‑140 (MQ=255)
                                                     tctGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGAAGACGGTCGAAAATTACGGCA‑GGCGGAGTTTGTTTTTTACAGaa                                                                                   >  7:638613/1‑140 (MQ=255)
                                                        gCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTAACAAGATGAGTGTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGCAAgg                                                                                >  8:315792/1‑140 (MQ=255)
                                                         cACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAATAATACCTTCGAAAATTCCGGCA‑GGCGCAGTTTTATTATTACAGAAAGGc                                                                               >  8:143605/1‑140 (MQ=255)
                                                             gtgtTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTTTCAAAATGACGTTCGAATATTACGGCA‑GGCGCAGGTTTTTTATTCCAGAAAGGCGGTg                                                                           >  6:208865/1‑140 (MQ=255)
                                                             gtgtTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCGTTATAAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTGCAGAAAGGCGTTg                                                                           >  8:631641/1‑140 (MQ=255)
                                                             gtgtTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGAGGTCCTTTATCAAGATGACGATAGAATAATCCGGCA‑CGGGCAGTTTGTTTATTACAGAAAGGCGTTg                                                                           >  5:237424/1‑140 (MQ=255)
                                                              tgtTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTTTCAAGATGACGTTCGAATATTACGGCC‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGa                                                                          >  4:1060641/1‑140 (MQ=255)
                                                              tgtTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGa                                                                          >  4:417491/1‑140 (MQ=255)
                                                              tgtTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGGTGCTTTATAAAGATGGAGTTCGAATATTACGGCA‑GGCGCAGGTTGTTTATTACAGAAAGGCCTTGa                                                                          >  6:169502/1‑140 (MQ=255)
                                                              tgtTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCACGTTCCTGTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTAGTCCAGAAAGGCGTTGa                                                                          >  3:618401/1‑140 (MQ=255)
                                                               gtTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAACGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAg                                                                         <  5:336815/140‑1 (MQ=255)
                                                               gtTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTACACCCCCGTGCGCGGTCCGTTATAAATATGACGTTCGAATATTCAGGCA‑GGCGCAGTTTGTTTATTACAGAAAGTCGTTg                                                                           >  2:602937/1‑138 (MQ=255)
                                                                ttCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTTTAAATATGACCTTCGAATATTACGGCA‑GGCGCAGTTTGTTTATTACAGAAAGGCGTTGa                                                                          >  2:344901/1‑138 (MQ=255)
                                                                ttCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTACGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGa                                                                          >  2:1050817/1‑138 (MQ=255)
                                                                ttCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTACTTTATCAAGATTACGTTCGAATATTCCGGCA‑GGCGCACTTTGTTTATTACAGAAAGGCGTTGa                                                                          >  1:932136/1‑138 (MQ=255)
                                                                ttCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGGTCCTTTATAAAAATGACGTTCGAATATTCCCGCA‑GGCGCAGTTTGTTTATTACCCAAAGGCGTTgt                                                                          >  2:213384/1‑137 (MQ=255)
                                                                 tcatcaGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAgcg                                                                       <  5:323330/140‑1 (MQ=255)
                                                                   atcaGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCATTATAAACAAGACGGTCGAATAATAAGGCA‑GTCGAAATTTTTTTATTACAGAAAGGCGTTGAGagcg                                                                     >  8:882327/1‑140 (MQ=255)
                                                                   atcaCCGTAGCCGAGATATTTGACCTCCCAATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGGTCCTTTATGAAGAGGACGGTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGGGcgcg                                                                     >  4:187307/1‑140 (MQ=255)
                                                                    tccgCGTAGCCGAGATATTTGACCGCCCCAATCATAACGGAAAGGCGGGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAgcgc                                                                      <  2:777365/135‑1 (MQ=255)
                                                                     caGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTCAACCCCCGGGCGCGGTCCGTTTTTAATATGAACTTAGAAAATTACGGCA‑GGCGCAGTTTGTTTATTACATAAAAGCGTTTAgagcc                                                                     >  1:245410/1‑137 (MQ=255)
                                                                     caGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTACGGCA‑GGCGCAGTTTGTTTATTACAGAAAGGCGTTGAGCGCGTa                                                                   >  5:271248/1‑140 (MQ=255)
                                                                      ctcGTAGCCGTGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGGTCCTTTATCAAGATGACGTTCGAATATTCCGTCA‑GGCTCATTTTTTTTATTCCAGAAAGGCGTTGAGCGAGTAt                                                                  <  8:183830/138‑1 (MQ=255)
                                                                      cgCGTAGCCGAGATATTTGACCGCCCCATGCATACCGGAAAGGCGTGGGTAACCCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGt                                                                    <  2:885576/137‑1 (MQ=255)
                                                                      cgCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGGGGGTAAACCCCCGGGCGCGGTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTAt                                                                  <  5:238000/139‑1 (MQ=255)
                                                                      cgCGTAGCCGAGAGATTTGACCGCCCCATGCATAACGGAACGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTCTCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAACGGCGTTGAGCGCGt                                                                    <  1:661762/137‑1 (MQ=255)
                                                                      aGCGTAGCCGAGATCTTTGACCGCCCCATGCATAACGGAAACGCGTGGGTAACCCCCCGGGCGCGTTCCTTTATCAAGATGACGATCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGt                                                                    <  1:1000750/138‑1 (MQ=255)
                                                                      aGCGTAGCCGAGATATTTTACCGCCACATTAATAACGGAAAGGCGTGGGTAAACCCCCTGGCGAGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCTCAGTTTTTTTATTCCAGAAAGGAGTTGAGCGCGTAt                                                                  <  4:546615/140‑1 (MQ=255)
                                                                      aGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTAt                                                                  <  8:374121/140‑1 (MQ=255)
                                                                      aGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTAt                                                                  <  4:372965/140‑1 (MQ=255)
                                                                      aGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAACATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGt                                                                    <  1:1034618/138‑1 (MQ=255)
                                                                        cGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTGCTTTATAAAGATGACGTTAGAATATTACGGCA‑GGCGGAGTGTTTTTATTGCATAAAGGCGTTTAGCGCGTATGa                                                                >  7:227923/1‑140 (MQ=255)
                                                                        cGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTACGGCC‑GGCGCAGGTTGTTTATTACAGAAAGGCGTTGCGCGCGTATGa                                                                >  4:518526/1‑140 (MQ=255)
                                                                        cGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATAAAGATTAAGTTAGCATATTACCGCACGGCG‑AGGTTTTGTTTTACAGAAAGGCGTTGAGCGCGTAtta                                                                >  3:452151/1‑138 (MQ=255)
                                                                        cGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGGTGCTTTATCAAGATGACGGTCGAATATTACGGCC‑GGCGCAGTTTGTTTATTCAAGAAAGGCGTTGAGCGGGTATGa                                                                >  3:476701/1‑140 (MQ=255)
                                                                        cGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGGTCCTTTATCAAGATGACGTTCGAATATTCAGGCA‑GGCGGAGGTTGTTTATTGCAGAAAGGCGTTGAGCGCGTAtta                                                                >  5:361638/1‑138 (MQ=255)
                                                                        cGTAGCCGAGATATTTGACCGCCCCATACATAAAGGAAAGGCGTGGGTAAACCCCCGGGCGCGGTCCAATATAAATGTGAAGGAAGAATATAACGGAA‑CGGGAACAATGTTAATACCAGAAATCCGATGACCGCGTATGa                                                                >  4:825524/1‑140 (MQ=255)
                                                                          tAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCAATTATAAAAAAGACCTTCGAATATTCCGGCA‑GTCGCCCTTTGTTTTTTACACAAAGGCGGTGAGCGCGAAAGAat                                                              >  5:797919/1‑140 (MQ=255)
                                                                          tAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTACTTTTTGAAGATGACGTTCGAATATTACGGCA‑GGGGGATGTTGTTGTTTGCATAAAGGCGGTTCGCGCGGATgt                                                                >  2:458798/1‑137 (MQ=255)
                                                                          tAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGGTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTGCAGAAAGGCGTTGAGCGCGTATGa                                                                >  2:428111/1‑138 (MQ=255)
                                                                          tAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGGTCCATTATAAACATGACGTTGGAATTTTCCGGCA‑GGCGCAGGTTGTTTTTTACAGAAAGGGGTTGAGCGCGTTTgg                                                                >  1:963242/1‑137 (MQ=255)
                                                                           aGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGaa                                                               <  1:884557/138‑1 (MQ=255)
                                                                           aGCCGAGATATTTGACCGCCCCAAGCATAACGGACAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTTAGCGCGTATGaa                                                               <  2:587695/138‑1 (MQ=255)
                                                                                agaTATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAttt                                                        <  4:1048427/140‑1 (MQ=255)
                                                                                  atatTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGGTCCTTTATCAAGATGACGGTCGAATATTCCGGCA‑GGCGCAGTTTGGTTATTGCAGAAAGGCGTTGAGCGCGGATGAATATAATTCt                                                      >  6:617224/1‑140 (MQ=255)
                                                                                    atTTGACCGCCCCATGCATCACGGAAAGGCGTGGGTAAACCCCCGGGCGCGGTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCtgt                                                    <  7:993301/140‑1 (MQ=255)
                                                                                         cccGGCCCAGTCATTACGGTAAGGCGTTGGTAAACACCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCAAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGAt                                               <  7:413910/139‑1 (MQ=255)
                                                                                             ccccATGCATAACGGAAAGGCTTTGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTGCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATAAAATTCGGGGGGATTTgg                                           >  8:836654/1‑139 (MQ=255)
                                                                                             ccccATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCC‑GGCGCAGTTTGTGTATTACAGAACGGGGTTGAGCGCGTATGAATATAATTGTGTGGGTTTTgc                                           >  4:589509/1‑140 (MQ=255)
                                                                                             ccccATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTACTTTATCAAGCTGACGTTCGAATATTACGGCC‑CGCGCAGATTGTTTTTTACAGAAAGGCGGTGAGCGCGTATGAATTTTATTGTGTTGGAttttg                                           >  7:775797/1‑138 (MQ=255)
                                                                                             ccccATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGGTGCTTTTTCAAGATTGCGTTCGAATATTCCCGCA‑GTCGCAGTTTGTTTATTCCAGAAAGGCGGTGCGCGCGGATGCATATAATTCTGTGGGATTTgg                                           >  3:891241/1‑139 (MQ=255)
                                                                                             ccccATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGGTCCTTTATAAAGATTCCGGTAGAATATTCCGGCC‑GGCGCAGTTTGTTTTTTACACAAAGGCGTTGAGCGCGGATTAATATAAATATTTGGGTTTTgc                                           >  3:647470/1‑140 (MQ=255)
                                                                                               ccATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTgc                                           <  2:256637/138‑1 (MQ=255)
                                                                                               ccATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTACGGCA‑GGCGCAGTTTGTTTATTACAGAAAGGCGTTGAGCGCGTATGAATATAATTATTTGGGAttttg                                           >  1:903054/1‑136 (MQ=255)
                                                                                                cATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTgca                                          <  1:494716/138‑1 (MQ=255)
                                                                                                  tGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGCAGCAt                                      <  5:733441/140‑1 (MQ=255)
                                                                                                       aaCGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTTACAATAAGACGTTCGAATATTACCGCA‑GGCGCAGTTTGTTTATTCCAGAAATGCGTTGAGCGCGTATGAATATTATTAATTGGGAATTGCAGCATACttt                                 >  8:451364/1‑140 (MQ=255)
                                                                                                        aCGGAAAGTCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGGTCGAATATACCGGCA‑GGCGCAGTTTGTTTATTGCAGAAAGGCGTTGCGCGCCTATGAATATAATTCTGTGGGATTTTCAGCATACttat                                >  4:289986/1‑138 (MQ=255)
                                                                                                        aCGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTACGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGCAGCATCCtttt                                >  4:180931/1‑140 (MQ=255)
                                                                                                        aCGGAAAGGCGTGGGTAAACCCCCGGGCGCGGTCCTTTATCAAGATGTCGTTCGCATATTCCCGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGATCGCGTTTTTATATAATTCTGTGGGATTTGCAGCATCCtttt                                >  3:1011560/1‑140 (MQ=255)
                                                                                                         cGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATGAAGATGACGGTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTACAGAAAGGCGTTGGGCGCGTATGAAAATTATTATGTGGGATTTTCAGCATCCgttta                               >  6:404830/1‑139 (MQ=255)
                                                                                                          tgAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCT‑GGCGCAGTTTGTGTATTCGAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGCAGCATCCTTTTcc                              <  3:115457/139‑1 (MQ=255)
                                                                                                          ggAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGCAGCATCCTTTTcc                              <  5:999414/140‑1 (MQ=255)
                                                                                                          ggAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGCAGCATCCTTTTcc                              <  3:180931/140‑1 (MQ=255)
                                                                                                          ggAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGCAGCATCCTTTTcc                              <  3:259277/140‑1 (MQ=255)
                                                                                                          ggAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGCAGCATCCTTTTcc                              <  4:679294/140‑1 (MQ=255)
                                                                                                          ggAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATACCGGCA‑GGCACAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGCAGCATCCTTTTcc                              <  3:826160/140‑1 (MQ=255)
                                                                                                          ggAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGGTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTATGTGGGATTTGCAGCATCCtttt                                >  2:967128/1‑138 (MQ=255)
                                                                                                          ggAAAGGCGTGGGTAAACCCCCGGGCGCGTTACTTTATCAAGATGGCGTTAGAATATTACGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGGAGCATCCtttt                                >  2:197812/1‑138 (MQ=255)
                                                                                                              aGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGCAGCATCCTTTTCcct                            <  1:344901/138‑1 (MQ=255)
                                                                                                              aGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTACGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGGATGAATATAATTCTGTGGGATTTGCAGCATCCTTTTCcct                            >  2:383932/1‑138 (MQ=255)
                                                                                                                     ggTAAACCCCCGGGCGCGTTACTTTATAAAGATTACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCCGATGAATATAATTATGTGGGGGTTGGACCATCCTTTTCCCTACTTggg                     >  2:505912/1‑138 (MQ=255)
                                                                                                                          aCCCCCGGGCGCGTTGCTTTTTCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTACAGAAAGGCGTTTAGCGCGTATGAATATAATTCTGTGGGTTTTGGAGCATCCTTTTCCCTCCTTCGGTGCATgc              >  5:821496/1‑140 (MQ=255)
                                                                                                                          aCCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTACGGCA‑GGCGCAGTTTGTTTATTACAGAAAGGGGTTGGGCGCGTATGAATATAATTATGTGGGATTTGCAGCATCCTTTTCCCACCTTCGGTGAATgc              >  5:656239/1‑140 (MQ=255)
                                                                                                                             cccGGGGGCGTTCCTTTATCAAGATGACGTTCGTATATTCCGGCA‑GGCGCAGATTGTTTATTAGAGATAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTTCAGCATACTTTTCCCTCCTTCGGTGTATGCGCt           <  7:956688/140‑1 (MQ=255)
                                                                                                                               cGGGCGCGTTCCGTTATCAAGATGACGTTCGAATATTACGGCA‑GGCGCAGTTTTTTTATTACAGAAAGGCGTTGAGCGCGTATGAATATAATTATGTGGGATTTGCAGCATCCTTTTCCCACCTTCGGTGAATGCGCTGa         >  6:471927/1‑140 (MQ=255)
                                                                                                                                gggCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGCAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGaa        <  4:101566/140‑1 (MQ=255)
                                                                                                                                 ggCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTATATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGCAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGa         <  1:775463/138‑1 (MQ=255)
                                                                                                                                   cgcgTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTACAGAAAGGCGTTGAGCGCGGATGAATATAATTGTGTGGGATTTGCAGCATCCTTTTCCCTCCTTCGGTGAATGCGGTGaaa       >  2:42129/1‑138 (MQ=255)
                                                                                                                                      gTTCCTTTCTCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGCAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGt  <  4:354319/140‑1 (MQ=255)
                                                                                                                                      gTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGCAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGt  <  8:440756/140‑1 (MQ=255)
                                                                                                                                      gTTCCGTTATCAAGATGACGGTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGGATATAATTCTTTTGGATTTGCAGCATCCATTTACCACCATCGGTGAATGCGCTGAAAACGGt  >  8:135960/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                           
CTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACGTTCGAATATTCCGGCA‑GGCGCAGTTTGTTTATTCCAGAAAGGCGTTGAGCGCGTATGAATATAATTCTGTGGGATTTGCAGCATCCTTTTCCCTCCTTCGGTGAATGCGCTGAAAACGGT  >  NZ_CP022686/360092‑360365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑