Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,115,447	0	C	G	29.8%	110.5 / 20.8	70	S243W (TCG→TGG)	lptG	Lipopolysaccharide export system permeaseprotein LptG
Reads supporting (aligned to +/- strand):  ref base C (17/30);  new base G (20/0);  total (40/30)
Fisher's exact test for biased strand distribution p-value = 3.10e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCCGATGTGCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTAACGGCGGCCCTGGTGCTGATGGCGATTTCG  >  ACB122/1115320‑1115580                                                                                                                                |                                                                                                                                       gCCGATCTGCGTACCACCTACTTCCGCGGCGACCACACCTAGGTGGTCAACGCGACGGAGGCCCGCTGGGATGTTTCGCTCACGAGAGATCTGCTCAATACCGTGATCCTCGCGCGGGAGTCGCGTTAGA‑tgagccg                                                                                                                              >  6:364928/1‑131 (MQ=255)  gcGATTTGGGTACCTCCTACTTCCGGGGCGACCACTGCGAGGTTGTCTACGCTGCGGAGGAGCGCTGGGTTGTTTCGCTAACGCCATAACTTCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCggg                                                                                                                             <  5:123417/136‑1 (MQ=255)  ccGATGTGCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGGCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCggg                                                                                                                             <  3:200455/137‑1 (MQ=255)  ccGATGTGCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCggg                                                                                                                             <  2:41158/137‑1 (MQ=255)      ggtgCGTACCACCTACTTCCGCGGCGACCACCCCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGgtgt                                                                                                                         <  5:449685/136‑1 (MQ=255)        tgCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGCCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTgg                                                                                                                       <  8:337024/137‑1 (MQ=255)         gCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTggg                                                                                                                      <  5:439416/137‑1 (MQ=255)         gCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTggg                                                                                                                      <  2:39541/137‑1 (MQ=255)              ccaccTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTAc                                                                                                                 <  5:184391/137‑1 (MQ=255)                accTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑tt                                                                                                                                   <  2:177820/117‑1 (MQ=255)                accTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTGTGGA‑tt                                                                                                                                   >  1:177820/1‑117 (MQ=255)                  cTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCGTTCGA‑TTTCCGGGTTGTGGGGTTACATcc                                                                                                             >  8:244972/1‑137 (MQ=255)                   tACTTCCGCGGCGACCACACCGCGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCa                                                                                                            <  1:250475/137‑1 (MQ=255)                       tCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTGTGGC‑ATAACGGGTTTTTGGATTAAAAGCactaa                                                                                                        >  2:264741/1‑136 (MQ=255)                       tCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCGTTGGA‑TTTACGGGTTGTGGGGTTTAATCCactac                                                                                                        >  7:26137/1‑137 (MQ=255)                       tCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCGTTCGA‑TTACCGGGTTGTGGGATTAAATGCcctac                                                                                                        >  4:318257/1‑137 (MQ=255)                         cgcgGCGACCACACCGAGGTGGTGAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACtt                                                                                                      <  5:230672/137‑1 (MQ=255)                          gcggcgACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCGTTGGA‑TTACCGGGTTGTGGGATTACATCCACGACATg                                                                                                     >  6:201929/1‑137 (MQ=255)                              cgACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGTTTAAATCCACTAATTGTCTg                                                                                                 >  7:239544/1‑137 (MQ=255)                                   acacCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTGGA‑TTAACGGGTTGTGGGATTACATCCACTCCTTGTCTGATCAg                                                                                            >  8:79038/1‑137 (MQ=255)                                   acacCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTGGA‑TTAAACGGTTGTGGGGTTTGATCACCTACTtgtgtggtaat                                                                                            >  1:127977/1‑132 (MQ=255)                                   acacCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAg                                                                                            <  2:422562/137‑1 (MQ=255)                                         aGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCGTTGGA‑TTTACGGGTTGTTGGTTTTAATCCACTACTTTTCTGAGAAGGGCCTg                                                                                      >  2:91364/1‑137 (MQ=255)                                            tggtCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCGTTGGA‑TTTCCGGGTTGTGGGTTTTAATCCACAACGTGTGGGATAATGGCCGGaca                                                                                   >  6:54588/1‑135 (MQ=255)                                             ggtCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCCGGTTTTGGGATTAAATACACTACGTGTGTGATCACGGCCTGaaca                                                                                  >  5:448915/1‑137 (MQ=255)                                               tCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGaacaac                                                                                >  6:84047/1‑137 (MQ=255)                                               tCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCGTTCGA‑TTAACGGGTTGTGGGTTTTAAAACACTAAATTTCTGATAACGGGCTGaaacac                                                                                >  4:305439/1‑133 (MQ=255)                                                 aaCGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTAACGCGTTGTGGGATTAAATACAATAATAGTCTGATCATGGCCTGAAAAAATc                                                                              >  8:134119/1‑137 (MQ=255)                                                      gcCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCGTTGGG‑TTACCGGGTTGTGGGGTTTAATACACTACTTGTGTGATCAGGGCCTGGAAAACTCCCGcg                                                                         >  3:276868/1‑136 (MQ=255)                                                                   tGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCCCTACTTGTATGATAAGGGGCTGAACAACTCCCGCTACTGGCTGGGGtt                                                            >  4:123603/1‑137 (MQ=255)                                                                   tGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAATCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTActgg                                                                    >  3:72418/1‑129 (MQ=255)                                                                   tGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAATCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTActgg                                                                    <  4:72418/129‑1 (MQ=255)                                                                     ggATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCGTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCt                                                          >  2:31443/1‑137 (MQ=255)                                                                        tGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGa                                                       <  4:263280/137‑1 (MQ=255)                                                                                  aCGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCGTTGGC‑TTTCCGGGTTGTGGGATTAAATCCACTAATTTTCTGATAAGGGGCTGAACAACTCCCCCTAATGGCGGGCGTTCTGGACCAAGGtgct                                             >  6:396531/1‑137 (MQ=255)                                                                                    gCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGtgctgc                                           <  3:79554/137‑1 (MQ=255)                                                                                     ccAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCa                                          <  5:84047/137‑1 (MQ=255)                                                                                     ccAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCa                                          <  1:91364/137‑1 (MQ=255)                                                                                     ccAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCa                                          <  6:343787/137‑1 (MQ=255)                                                                                      cAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAg                                         <  7:376909/137‑1 (MQ=255)                                                                                              cTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGt                                 <  6:93067/137‑1 (MQ=255)                                                                                              cTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGt                                 <  8:58063/137‑1 (MQ=255)                                                                                                 aaTACCGTGTTCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGTATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTAAc                              <  5:230141/137‑1 (MQ=255)                                                                                                 aaTACCGTGATCCTCGCGCCGGAGTCGCTTTTGA‑TTACCCGGGTTTGGGATTAAAAACACATAATGTCTGTAAAGGGCCTGCAACACTCCCGCTACTGGGGGGCCGTCTTGACCAAGTTGGGTCAGCCCCTGGTaaa                              >  4:309056/1‑136 (MQ=255)                                                                                                 aaTACCGTGATCCTCGCGCCGGAGTCGCTTTGGA‑TTACCGGGTTGTTGGATTTCATACCCTACTTGTCTGATAAGGGGCTGAACAAATCCCGCTACTGGCTGGGGTTATGGACCAAGGTGCTGCCCCCGCTGGttaa                              >  8:288264/1‑134 (MQ=255)                                                                                                 aaTACCGTGATCCTCGCGCCGGAGTCGCTTTGGA‑TTAACGGGTTGTGGGATTACATCCACTACTTGTGTGATAAGGGCCTGAACAACAACCGCTACTGGCTGGCGTTGTGGACCAAGGTGCTTCAGCCGATGGTAAc                              >  4:339616/1‑137 (MQ=255)                                                                                                 aaTACCGTGATCCTCGCGCCGGAGTCGCTTTGGA‑TTAACGGGTTGTGGGATTACATCCACTACTTGTCTGATCATGGGCTGCACAAATACCGCGACTGGCTGGCGGTCTGGACCAAGGTGGTGCAGCCGAAGGTaaa                              >  4:170988/1‑136 (MQ=255)                                                                                                 aaTACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTAcc                                                                                                                                <  7:433945/39‑1 (MQ=255)                                                                                                 aaTACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTAcc                                                                                                                                >  8:433945/1‑39 (MQ=255)                                                                                                 aaTACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGTATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTAAc                              <  1:13340/137‑1 (MQ=255)                                                                                                 aaTACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTAAc                              <  1:240569/137‑1 (MQ=255)                                                                                                 aaTACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TCACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTaaa                              >  6:66455/1‑136 (MQ=255)                                                                                                 aaTACCGTGATCCTCGCGCCGGAGTCGCTTTAGA‑TTACCGGGGTGTGTGCTTAACTACACTAAGAGTCTGATCAGTGCCAGTAAAACTCCCGCTACATGCAGGAGATGTGTACCAACGTGCTGGAGCGGAttctcac                              >  8:350857/1‑131 (MQ=255)                                                                                                 aaTACCGTGATCCTCGCGCCGGAGTCGCTGTGGA‑TTACCGGGTTTTGGGTTTACATACAATAATTGTCTGATCAGGGCCGGAACAACTACCCCGACTGGCTGGCGTTATGGGCCCACGGGCGGCAGCCGCTGGTAAc                              >  2:376328/1‑137 (MQ=255)                                                                                                 aaTACCGTGATCCTCGCGCCGGAGTCGCGTTGGT‑TTAACGGGGTGTGGGGTTTAATACCCTACATGTGTGATAAGGGCCTGAACAACACCCCCCACTGGCGGGCGTGGTGGTCCCAGTTGCGTCACCCGCTgggtaa                              >  6:251759/1‑133 (MQ=255)                                                                                                 aaTACCGTGATCCTCGCGCCGGAGTCGCGTTGGA‑TTACCGGGTTGTGGGATTACATCCAAAACATGTCTGCACAGGGCCTGAACAACTCCCGCTACTGGCTGGCGGTCTGGCCCAAGGTGCTGCAGCCGCTGGTaaa                              >  8:99911/1‑136 (MQ=255)                                                                                                 aaTACCGTGATCCTCGCGCCGGAGTCGCGTTCGA‑TTTACGGGTTGTTGGTTTTCATACACAACTTGTGTGATCAGGGCCTGAACAAATACCCCTACTGGCTGGCGGTCTGGACCAAGGTGCTGCAGCCGCTGGTaaa                              >  4:79554/1‑136 (MQ=255)                                                                                                 aaTACCGTGATCCTCGCGCCGGAGTCGCGGTCGA‑TTACCCGGTTGTGGGATTAGATCCCCGAATTTTCGGAAAACGGCCAGAACAACACCCCCAACTGGCAGGCGGGCTGGACCCAGGTGCTGCAGCCGAGGGTAAc                              >  6:161756/1‑137 (MQ=255)                                                                                                   tACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGTATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTAAcgg                            <  6:103778/137‑1 (MQ=255)                                                                                                     ccGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTAAcggcg                          <  3:214256/137‑1 (MQ=255)                                                                                                     ccGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTAAcggcg                          <  1:439907/137‑1 (MQ=255)                                                                                                      cGTGATCCTCGCGCCGGAGTCGCTTTGGT‑TTTACGGGTTTTGGGTTTTAATCAAAGAATTTTATGTTAATGGCCGGAAAAACACCCGCGACTGGCTTGGGTTGTTGGCCAAGGTGCTGAAGACGATGGTTAaggcgg                         >  4:398/1‑137 (MQ=255)                                                                                                      cGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTTCCGGGTTTTTGGATTACATAAAATAATTGTATGATAAGGGGCTGCACAAAAACCGCAACATGCGGGCGTTGTTGACCAAGGTGCTGCACCCGATGGTAAaggcgg                         >  8:52909/1‑137 (MQ=255)                                                                                                      cGTGATCCTCGCGCCGGAGTCGCGTTCGA‑TTAACGGGTTGTGGGATTAAAAACAAAAATTTTCTGAAAAAGGCCTGAAAAAAAACCCCAACAGGCTGGCCTTCAGGACCAAGGTGCTGCAGCCGATGGTAAacgcgg                         >  4:202912/1‑137 (MQ=255)                                                                                                           tCCTCGCGCCGGAGTCGCTTTCGA‑TTAACCGGTTGTGGGGTTACATCCACTACATGTCTGATAAGGGCCTGAACAACAACCGCTACAGGATGGCGGTATGGACCAACGTGCTGCAGCCCATGGTAACCGCGGcccag                    >  6:427336/1‑135 (MQ=255)                                                                                                           tCCTCGCGCCGGAGTCGCGTTGGATTTGCCGGGTT‑TGGGGTTTCATACCCGAATTGTGTGTTCACGGGCGGGACAAAGCCCCCGACTGGCTGGGGGTGTGGGCCAAGGTGCTGCAGCCGCTGGTAACCGGGGcccgg                    >  4:38810/1‑135 (MQ=255)                                                                                                           tCCTCGCGCCGGAGTCGCGTTGGA‑TTACCGGGTTGTGGGCTTACATCCACGACTTGTCTGATCAGGGCCTGAACAACTCCCGCGACTGGCTGGCGGTCTGGCCCAAGGTGCTGCCGCCGCTGGTAACGGCGGcccgg                    >  2:242794/1‑135 (MQ=255)                                                                                                           tCCTCGCGCCGGAGTCGCGTTGGA‑TTACCGGGGTGTGGGGTTTCATCCACTACTTGTGGGGTGAGGGGCTGAACAACTCCCGCGACGGGCGGGCGGTGTGGGCCAAGGTGCTGCCGCCGCTGGTAACGGCGGcccgg                    >  4:364634/1‑135 (MQ=255)                                                                                                                       aGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTAACGGCGGCCCTGGTGCTGATGGCg        <  8:26137/137‑1 (MQ=255)                                                                                                                             tttCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTAACGGCGGCCCTGGTGCTGATGGCGATTTCg  <  3:202912/137‑1 (MQ=255)                                                                                                                                |                                                                                                                                       GCCGATGTGCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGA‑TTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTAACGGCGGCCCTGGTGCTGATGGCGATTTCG  >  ACB122/1115320‑1115580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑