| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,537,415 | 0 | C | G | 34.5% | 36.6 / 15.2 | 30 | A388G (GCG→GGG) | mdfA | Multidrug transporter MdfA |
| Reads supporting (aligned to +/- strand): ref base C (18/1); new base G (0/10); total (19/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.49e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGAACAGAGCAAGGGGCTGGTTTCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTG‑CC‑C‑TGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCT > ACB122/1537290‑1537541 | ctaaCAGTGAAAGGGGCTGGTTTGGGCCTTGGTGGGTATTATCCCTATTGCCTTGCTGGGGTTTGGCGGAGCGCGGGTGGGTATGATTGTCGCGGGTGCCTGTTTGCTGCTTTTTG‑CC‑C‑TGGGGGGGGGGGTGgggg < 6:36019/135‑1 (MQ=255) agagCAAGGGGCTGGTTTCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTG‑CC‑C‑TGGCGGCGGGGGTGGCGGGGGCg > 5:344910/1‑137 (MQ=255) aaGGGGCTGGTTTCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTG‑CC‑C‑TGGCGGCGGGGGTGGCGGGGGCGTTGgc > 8:109022/1‑137 (MQ=255) aaGGGGCTGGTTTCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTG‑CC‑C‑TGGCGGCGGGGGTGGCGGGGGCGTTGgc > 2:393784/1‑137 (MQ=255) ggCTGGTTTCGGCCATGCGGGGTATGATCACTATTGCCTTGCTGGGGTTTGCCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTG‑CC‑C‑TGGCGGCGGGGGGGGCGGGGGCGTTGGCGCtg < 4:162640/137‑1 (MQ=255) gCTGGTTTCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTG‑CC‑C‑TGGCGGCGGGGGTGGCGGGGGCGTTGGCGCtgt > 2:54614/1‑137 (MQ=255) gCTGGTTTCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTG‑CC‑C‑TGGCGGCGGGGGTGGCGGGGGCGTTGGCGCtgt > 2:61023/1‑137 (MQ=255) gCTGGTTTCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTG‑CC‑C‑TGGCGGCGGGGGTGGCGGGGGCGTTGGCGCtgt > 5:193419/1‑137 (MQ=255) gTTGCGGCCAGGGGGGGGTTGATCACGATTGCCTGGGGGGGGTTTGGCGGCGCGCGGCTGGCTTTGTTGGGGGCCGGTGCCAGGTTGGTGCTTTTTC‑CC‑C‑TGGGGGGGGGGGTGGGGGGGGCGTTGGCGCTGTGGcc < 8:105139/137‑1 (MQ=255) tttCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTG‑CC‑C‑TGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCg > 1:222786/1‑137 (MQ=255) tttCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTG‑CC‑C‑TGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCg > 3:130555/1‑137 (MQ=255) tttCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTG‑CC‑C‑TGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCg > 3:345966/1‑137 (MQ=255) gatCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTG‑CC‑C‑TGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTggtgggtg > 3:411057/1‑137 (MQ=255) aCGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTG‑CC‑C‑TGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGggcg > 1:149893/1‑137 (MQ=255) aCGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTG‑CC‑C‑TGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGggcg > 6:72806/1‑137 (MQ=255) aCGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTG‑CC‑C‑TGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGggcg > 4:314706/1‑137 (MQ=255) aCGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTG‑CC‑C‑TGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGggcg > 1:121737/1‑137 (MQ=255) cGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGAGCCGGTGCCAGCTTGCTGCATTTTG‑CC‑C‑TGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGggcgg > 2:366960/1‑137 (MQ=255) gCCTTGCTGGGGTTTGGCGGAGCCCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTG‑CC‑C‑TGGCGGAGGGGGTGGCGGGGGCGTTGGCTCTGTGGCCGCTGTGGATTGTGGTGGGTGGGCGGCCTgg > 8:374771/1‑137 (MQ=255) gCCTTGCTGGGGTTTGGCGCAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTG‑CC‑C‑TGGCGGCGTGGGTGGCGCGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTgg > 7:55817/1‑137 (MQ=255) gcgcCGGTGCCAGCTTGCTGCATTTTG‑CC‑C‑TGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGggccg > 6:311763/1‑137 (MQ=255) tGCCAGCTTGCTGCATTTTG‑CC‑C‑TGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGggccgggggggg > 7:209111/1‑131 (MQ=255) tgggtttttG‑CC‑CGGGGGGGGGGGGGGGGGGGGGGGGTGGGCGCTGGGGCCGCGGTGGTTTGTGGTGGGTGGGCGGCCGGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 1:61023/132‑1 (MQ=255) ggtgaTTTTGCCC‑C‑GGGGGGGGGGGGGGGGGGGGGCGTGGGCGCGGGGGCCGGTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 7:109022/133‑1 (MQ=255) ggtctttttG‑CCGC‑GGGGGGGGGGGGGCGGGGGGGTGTTGGCGCGGGGGCGGCTGGGGTTGGTGGTGGGGGGGCGGCGTGGTGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 5:69344/132‑1 (MQ=255) gctgtttttG‑CC‑CGGGGGGGGGGGGGGGGGGGGGGCGTGGGCGCGGGGGCCGCTGTGGTTTGGGGGGGGTGGGCGGCCGGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 2:228015/132‑1 (MQ=255) gccgaTTTTG‑CC‑CGGGGGGGGGGGGGGGGGGGGGGGGTGGGCGCGGTGGCCGCTGTGGTTTGTGGGGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 3:314706/133‑1 (MQ=255) taTTTTG‑CC‑CGGGGGGGGGGGGGGGGGGGGGGGGTGGGCGCGGGGGCCGGGGGGGTTTGTGGGGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAAcc < 5:242092/136‑1 (MQ=255) ttttgG‑CC‑G‑TGGGGGGGGGGGGGGGGGGGGCGGTGGCGGTGGGGCGGCGGGGGTTTGTGGGGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCg < 8:128316/136‑1 (MQ=255) cccgtGGGGGGGGGGTGGGCGGGGGGTTTGGCGGGGGGGCGGGTGGGGTTTGTGGTGGTGGCGGGGCCTGGGGACGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCt < 2:173359/133‑1 (MQ=19) | CGAACAGAGCAAGGGGCTGGTTTCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTG‑CC‑C‑TGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCT > ACB122/1537290‑1537541 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |