| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 5,223,159 | 0 | G | C | 34.2% | 42.6 / 12.3 | 38 | intergenic (‑133/+43) | cntO_7/CJ019_04917 | Metal‑pseudopaline receptor CntO/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (8/17); new base C (13/0); total (21/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.20e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCCT‑A‑C‑GGGGGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGC > ACB122/5223028‑5223291 | gACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCCt < 8:103012/137‑1 (MQ=255)gACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATAGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGAGGCGGCCTCTTCCCGGGG‑TAAGcccccccc > 8:8096/1‑137 (MQ=255) aCCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TTAAACCCCCCCC‑A‑C‑cgggg > 3:173281/1‑137 (MQ=255) aCCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCCT‑A‑C‑ggggg < 8:196059/137‑1 (MQ=255) aCCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCCT‑A‑C‑ggggg < 8:245991/137‑1 (MQ=255) aCCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTCTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCCT‑A‑C‑ggggg < 1:400972/137‑1 (MQ=255) gtgAGGGTGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCCC‑C‑C‑GGGGGGttt < 6:343326/137‑1 (MQ=255) aGGGGGTGATTATTGCGAATCAATCTCACTTTCACTCATTACACGGATGACACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCCA‑A‑C‑GGGGGGTTTAcc > 8:299700/1‑137 (MQ=255) aGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCCT‑A‑A‑GGGGGGGTTaac > 8:305955/1‑135 (MQ=255) gggggTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGTTAAACCCCCCCAA‑A‑G‑GGGGGGTTTAcc > 5:304948/1‑137 (MQ=255) tGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGTTAAACCCCCCCCT‑C‑C‑GGGGGGTTTAccgggtt > 2:403225/1‑132 (MQ=255) attattGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TTAACCCCCCCCA‑A‑C‑CGGGGGTTTTAACGTGAagt > 5:347664/1‑136 (MQ=255) attattGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCCCCCCT‑A‑C‑GGGGGGGTTTCCCGTGAagg > 6:71219/1‑136 (MQ=255) attGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCCt < 8:391034/112‑1 (MQ=255) attGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCCt > 7:391034/1‑112 (MQ=255) gAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGTTAAACCCCCCCCA‑A‑G‑GGGGGGTTTACCCG‑GAAAAGCCTGGcc > 8:284390/1‑136 (MQ=255) gAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCCT‑A‑A‑GGGGGGTTTACCCGGGAAGAGGCTgggc > 2:275290/1‑135 (MQ=255) atcaatcCCACTTTCCCTCATTACCCGGATGCCACGGGCTGTTACCTCACCTGTCCCTGGAAATATTTTTGCGGGGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCCT‑A‑C‑GGGGGGTTTACCCGTTAAGAGGCTGGcaca < 7:284961/137‑1 (MQ=38) aatcGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCCCCCCT‑ACC‑GGGGGGTTTACCC‑TGTAGAGGCTGGGACAGGc > 7:329446/1‑137 (MQ=255) cACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGGAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAAACACCCCCA‑A‑C‑GGGGGGTTTAACCGTGAATAGGGTGGCACACGCAACTa > 6:392167/1‑137 (MQ=255) tttCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGTAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGT‑TAAACCCGCCCCT‑A‑C‑GGGGGGTTTACCCGTGAATAGGCTGGCACAGGCAACTACCg < 4:160595/137‑1 (MQ=255) acacGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TTAACCCCCCCCTAA‑C‑GGGGGGTTTA‑CCGTGTAGAGGCTGGCACAGGCAAATACCCAACCAGGGGCa > 6:185208/1‑137 (MQ=255) tGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TTAACCCCCCCCA‑A‑C‑GGGGGGGTTAAACGTGAAGAGGGTGGGCCACGCAACTAACGAACCCCGGGGAAACCCCCCAACCCgggg > 5:73098/1‑135 (MQ=255) ttACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TTAAACCCCCCCG‑A‑A‑GGGGGGGTTTCCCGGGTAGAGGCTGGCCCCGGCAACTACCGCACCAGGGGCAAACGCCCCAACACGGtgtg > 1:60419/1‑137 (MQ=255) aCCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TTAACCCCCCCCA‑A‑C‑CGGGGGGTTTCCCCTGAAGAGGCGGGGACCTGGCACAACCGCACCCAGGTGAAACCCCCCAACACGGGGTGcc > 4:215364/1‑137 (MQ=255) cTCACCTGTGCCTGTAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCCT‑A‑C‑GGGGGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGt < 5:185208/137‑1 (MQ=255) tCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCCT‑A‑C‑GGGGGGTTTACCCGTGAAGAGGCGGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTg < 5:67636/137‑1 (MQ=255) acACCTGTGCCTGTAAATTTTTTTGCGGTGGGGCCCTCTGCGC‑GGG‑TAACCCCGCCCCT‑C‑C‑GGGGGGTTTACCGGTGAAGAGGCGGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTg < 3:241809/136‑1 (MQ=255) ttgCCTGGAAATATTTTTGCGGGGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCCT‑C‑C‑GGGGGGTTTACCGGTGAAGTGGCGGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAAc < 6:136044/136‑1 (MQ=255) gtgCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCCCCCCA‑A‑CGGGGGGGTTTACCCG‑GAAGAGGCGGGCACACGCACCAACCCAACCACGGGCAAAACCCCCCACACGGTCTGCCCGGAAAAAc > 4:92666/1‑137 (MQ=255) cTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TTAAACCGCCCCA‑A‑A‑GGGGGGGTTTACCGGGGAGCGGCTGGCCCAGGCAACGACCCCACCAGGGGGAAACGCCCCCACCCCGTCTGCCGGGCAAAACCCaa > 2:303566/1‑136 (MQ=255) cTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCCT‑A‑C‑GGGGGG‑TTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCAta > 3:43123/1‑136 (MQ=255) tgtaaaTATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCCT‑C‑C‑GGGGGGTTTACCCGTGAAGAGGCGGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATc < 6:346024/134‑1 (MQ=255) aaTATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCCT‑C‑C‑GGGGGGTTTACCGGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCt < 4:361694/137‑1 (MQ=255) ttttttGCTGGGGCGGCATCTTCGG‑GGG‑TAAACCCGCCCCG‑A‑G‑GGGGGGTTTACCTGGTAATAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGc < 8:127829/136‑1 (MQ=255) ttttGCTGTGGCGGCCTCTTCGC‑GGG‑TTAACCCGCCCCC‑A‑A‑GGGGGGGTTTACCGGGAAGGGGGGGGCCCATGGAACTACCGCACCAGGGGGAAACGCCCCAACCCCGTCAGGCGGGAAAAAACCAACACCATGccc > 2:272893/1‑137 (MQ=255) gtgGGGGCCTCTTCGC‑GGG‑AAAACCCGCCCCT‑A‑G‑GGGGGGTTTACCCGTGATGTGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAAt < 7:135969/137‑1 (MQ=255) gggcCTCTGCGC‑GTG‑TACACCCGCCCCT‑C‑G‑GGGGGTTTTACCGGTTATGTGGCGGCCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGGCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAg < 8:157998/136‑1 (MQ=255) aaccGCCCCT‑A‑A‑CGGGGGGTTACCCGTGAAGAGGCTGGCACACGCAACAACCCAACCACGGTGAAAACCCCCAAAACCGACGGCCCTGAAAAACCCCACACCTAGCCCAGCGAAACAACCGCCACCACCCGCGCGGc > 4:72290/3‑137 (MQ=255) | GACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCCT‑A‑C‑GGGGGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGC > ACB122/5223028‑5223291 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |