| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB122 | 690,144 | A→G | intergenic (+21/‑2438) | bauC → / → PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 690,144 | 0 | A | G | 83.3% | 10.6 / ‑4.5 | 13 | intergenic (+21/‑2438) | bauC/PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
| Reads supporting (aligned to +/- strand): ref base A (1/0); major base G (10/0); minor base C (2/0); total (13/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCTATGGCCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCC‑GTCAGGCC‑TCCCCGC‑TT‑TGTTTTTGGGGGTTGGTTG‑TTGCTGTTGGGGAGGAACTGTTTTGAG > ACB122/690013‑690211 | gCCTATGGCCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑Gggggggggc > 3:375442/1‑134 (MQ=255) aTGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGcg‑gcccggccgcgccc > 5:247679/1‑131 (MQ=255) ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGC‑GGCCGGGC‑Accccccg > 2:236039/1‑134 (MQ=255) ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGCGGCC‑CTGCGGCC‑CACCCGcg > 6:216597/1‑136 (MQ=255) ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGGC‑CGCACGCC‑ACCCCGcg > 8:212030/1‑136 (MQ=255) aCCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATCCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCGGGCAGGCG‑CCCCCCG‑TT‑TGTTTTTGGGggt > 6:165002/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCG‑GCCCGGCC‑CCCCCGC‑TTGTGTTTTTGGGtgttgttgt > 3:385060/1‑133 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGC‑GGGCCGGC‑GCCCCGC‑GG‑TGGTTTTTGGGGGTGggtg‑tt > 3:341650/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGC‑GGGAGGCC‑GCCCCCC‑GT‑TGTTTTTTGGGggtgggtggt > 4:293259/1‑135 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCC‑CGCCCGCC‑GCCCCCC‑TG‑TGGTTTTTGGGGGTGggtg‑tt > 5:147515/1‑137 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGCGGGC‑CTGAGGCC‑GCCCCCCGGT‑TGTTTTTGGgggtgggtgtttg > 8:375199/1‑132 (MQ=255) gTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGC‑GGGAGGCC‑CGCCCCC‑GG‑TGTTTTTTGGGGGTGGGTG‑TTTGGGTTGggggggggagg > 8:68476/1‑132 (MQ=255) ttGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGC‑GGCAGGGC‑TCCCCGG‑TT‑TGTTTTTTGGGGGTGGTTGTTTGTTTTTGGgggggacagt > 6:409853/1‑133 (MQ=255) gCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGC‑GGGAGGCCGGCCCCCG‑GT‑TGTTTTTGGGGGGTGGTGT‑TTGTGGTTGGGG‑GGGGCTGttttttt > 8:5788/1‑134 (MQ=255) | GCCTATGGCCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCC‑GTCAGGCC‑TCCCCGC‑TT‑TGTTTTTGGGGGTTGGTTG‑TTGCTGTTGGGGAGGAACTGTTTTGAG > ACB122/690013‑690211 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |