Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	5,241,188	A→C	21.3%	F455V (TTC→GTC)	CJ019_04935 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	5,241,188	0	A	C	21.3%	91.8 / 2.2	65	F455V (TTC→GTC)	CJ019_04935	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (29/20);  new base C (2/14);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 1.34e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGTACTGATCGTCCTGCACCAGGCACAGCAGATCCTTCAAGGGCGTTTGCGCACCATTGAGGCAAACCAGACCGGCGCTGATGACATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTCACACAGTGCGGCCATGGTCAAAGTGTCGTCATAGAGCGTACGGATCTGCTTGAC  >  ACB122/5241057‑5241311                                                                                                                                     |                                                                                                                            cAGGTACTGATCGTCCTGCACCAGGCACAGCAGATCCTTCAAGGGCGTTTGCGCACCATTGAGGCAAACCAGACCGGCGCTGATGACATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAAcgc                                                                                                                          >  3:177495/1‑135 (MQ=255)       cTGATCGTCCTGCACCAGGCACAGCAGATCCTTCAAGGGCGTTTGCGCACCATTGAGGCAAACCAGACCGGCGCTGATGACATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCgg                                                                                                                    >  8:328580/1‑135 (MQ=255)       cTGATCGTCCTGCACCAGGCACAGCAGATCCTTCAAGGGCGTTTGCGCACCATTGAGGCAAACCAGACCGGCGCTGATGACATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCgg                                                                                                                    >  4:256704/1‑135 (MQ=255)       cTGATCGTCCTGCACCAGGCACAGCAGATCCTTCAAGGGCGTTTGCGCACCATTGAGGCAAACCAGACCGGCGCTGATGACATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCgg                                                                                                                    >  7:282220/1‑135 (MQ=255)          aTCGTCCTGCACCAGGCACAGCAGATCCTTCAAGGGCGTTTGCGCACCATTGAGGCAAACCAGACCGGCGCTGATGACATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGt                                                                                                                  >  8:49645/1‑134 (MQ=255)            cGTCCTGCACCAGGCACAGCAGATCCTTCAAGGGCGTTTGCGCACCATTGAGGCACACCAGACCGGCGCTGATGACATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGCTCGACCGCCGCCGGGGGaa                                                                                                               >  8:136331/1‑135 (MQ=255)            cGTCCTGCACCAGGCACAGCAGATCCTTCAAGGGCGTTTGCGCACCATTGAGGCAAACCAGACCGGCGCTGATGACATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCTAC‑AGATCGACCGCCGCCGGGTGa                                                                                                                >  3:40652/1‑134 (MQ=255)            cGTCCTGCACCAGGCACAGCAGATCCTTCAAGGGCGTTTGCGCACCATTGAGGCAAACCAGACCGGCGCTGATGACATAGGCCTGCATTATACCCAGGCGTCCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGa                                                                                                                >  4:235880/1‑134 (MQ=255)             gTCCTGCACCAGGCACAGCAGATCCTTCAAGGGCGTTTGCGCACCATTGAGGCAAACCAGACCGGCGCTGATGACATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGaaa                                                                                                              >  2:151584/1‑135 (MQ=255)                 tGCACCAGGCACAGCAGATCCTTCAAGGGCGTTTGCGCACCATTGAGGCAAACCAGACCGGCGCTGATGACATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAAccc                                                                                                          >  5:52733/1‑135 (MQ=255)                 tGCACCAGGCACAGCAGATCCTTCAAGGGCGTTTGCGCACCATTGAGGCAAACCAGACAGGCGCTGATGACATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAAccc                                                                                                          >  8:125455/1‑135 (MQ=255)                           acaGCAGATCCTTCAAGGGCGTTTGCGCACCATTGAGGCAAACCAGACCGGCGCTGATGACATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACgcg                                                                                                >  5:48471/1‑133 (MQ=255)                           acaGCAGATCATTCAAGGGCGTTTGCGCACAATTGAGGCAAACCACACCGGCGCTGATGACATTGGCCTCAATTTACCCCTGGCGTGCGTGGTGAAAC‑AGTTCTAACGCCGCCGGGTGAAAACCCTGATGACGAg                                                                                                <  1:279736/135‑1 (MQ=255)                                       tCAAGGGCGTTTGCGCACCATTGAGGCAAACCAGACCGGCGCTGATGACATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTgg                                                                                    >  7:1512/1‑135 (MQ=255)                                       tCAAGGGCGTTTGCGCACCATTGAGGCAAACCAGACCGGCGCTGATGACATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATCACGAGCGGCCTGATTgg                                                                                    >  3:93171/1‑135 (MQ=255)                                           ggggGTTTGCGCACCATTGTGCCAAACCTGCCCGGCGCGGAGGACATTGGCGTGCTTTTCCCCCCGGGGGGCGGGGGGCAAC‑AGAGCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGgccag                                                                                <  5:145928/135‑1 (MQ=255)                                                tttGCGCACCATTGAGGCAAACCAGACCGGCGCTGATGACATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGgccagcgcca                                                                           <  3:30580/135‑1 (MQ=255)                                                 ttGCGCACCATTGAGGCAAACCAGACCGGCGCTGATGACATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACACCGCCGGGTGAAAACCCTGATGACGACCGGCCTGATTGgccagcgccag                                                                          >  1:338314/1‑135 (MQ=255)                                                   gcgcACCATTGAGGCAAACCAGACCGGCGCTGATGACATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGcc                                                                        >  4:354170/1‑135 (MQ=255)                                                       aCCATTGAGGCAACCCAGACCGGCGCGGTTGACATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGc                                                                    <  2:69949/135‑1 (MQ=255)                                                         ctttGCGGCAAACCAGCCCGGCGCTGATCACATGGGCCTGCATTTTCCCCTGGCGGGGGGGGTGAAAC‑AGATCGACCGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCAt                                                                  <  1:151584/133‑1 (MQ=255)                                                         ctttGAGGCAAACCAGCCCGGCGCTGATGACATTGGCCTGCTTTTTCCCCAGGGGTGCGTGGTCCAAC‑TGAGCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCAt                                                                  <  1:81701/133‑1 (MQ=255)                                                         cATTTAGCCAAACCACACCGGCGCGGATCACATTGGCCTGCATTTTCCCCAGGCGGGCGTGGTGCAAC‑AGAGCGACCGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCAt                                                                  <  4:40652/135‑1 (MQ=255)                                                         cATTGTGGCAAACCAGCCCGGCGCTGATGACATTGCCCTGCATTTTCCCCCGGCGTGCGGGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCAt                                                                  <  8:69275/135‑1 (MQ=255)                                                         cATTGCGGCAACCCACCCGGGCGCTCTTGACTTGGGCCTGCTTTTTCCCCAGGCGGGGGTGGTGCAAC‑AGATCTAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCAt                                                                  <  6:356243/135‑1 (MQ=255)                                                         cATTGAGGCACACCAGACCGGCGCTGATGACATTGGCCTGAATTTTCCCCAGGCGTGCGGGGGGCAAC‑AGATCGACCGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCAt                                                                  <  3:369322/135‑1 (MQ=255)                                                         cATTGAGGCAAACCAGACCGGCGCTGTTCACTTTGGCCTGCATTATCCCCAGGCGGGCGTGGCGCAAC‑AGCTCGACCGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCAt                                                                  <  3:185619/135‑1 (MQ=255)                                                         cATTGAGCAAACCCCGCCCGGCGCTCATGAAATGGGCCTGATTTTCCCCCGGGGGGGCGGGGT‑CAACAAGCTCCCCCGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCAt                                                                  <  6:32339/135‑1 (MQ=255)                                                         cATGGAGCCAAACCAGACCGGCGAGTAGCACATTGGCCTGCATTTTCCCCCGGCGGGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCAt                                                                  <  3:42277/135‑1 (MQ=255)                                                             gcggCACACCAGCCCGGCGCTTATGACATTGGCCGCCTTTTCCCCCCGGCGTGGGTGGGGCAAC‑AGCCCGACCGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCa                                                                <  7:359724/131‑1 (MQ=255)                                                              aGGCAAACCAGACCGGCGCTGATGACATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGc                                                             >  3:5985/1‑135 (MQ=255)                                                                  aaaCCAGCCCGGCGCTGATGACATTGGCCTGCATTTTCCCCAGGCGGGCGGGGTGCAAC‑AGATCGACCGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCt                                                         <  7:49645/135‑1 (MQ=255)                                                                   acccAGACCGGCGCGGATCCCATTGCCCGGCCTTTCCCCCGGGGGGGCGGGGAGAAAC‑AGAGCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTc                                                        <  1:245751/133‑1 (MQ=255)                                                                   aacatgACCGGCGCTGATGACATTGGCGTGCTTTTCCCCCCGGCGGGGGTGGTGCAAC‑TGATCGACCGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTc                                                        <  8:1512/130‑1 (MQ=255)                                                                   aaCCAGACCGGCGCTGATGACATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTc                                                        <  4:177495/135‑1 (MQ=255)                                                                   aaCCAGACCGGCGCGGATGACTTTTGCCGGAATTTTCCCCAGGCGTGGGGGTTCCAAC‑ACACCGACCGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTc                                                        <  3:235675/135‑1 (MQ=255)                                                                   aaCCACCCCGGCGCTGATGACATTGGCATGCAGTTTCCCCCGGCGTGCGGGGGGCAAC‑CGAGCGACCGCCGCCGGGTGAAAACACTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTc                                                        <  6:247758/135‑1 (MQ=255)                                                                      cAGACCGGCGCTGATGACATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTcac                                                      >  4:346818/1‑134 (MQ=255)                                                                      cAGACCGGCGCTGATGACATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTcac                                                      >  1:235084/1‑134 (MQ=255)                                                                            ggCGCTGATGACATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCAt                                                                  >  8:351234/1‑116 (MQ=255)                                                                            ggCGCTGATGACATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCAt                                                                  <  7:351234/116‑1 (MQ=255)                                                                            ggCGCTGATGACATTGGCCTGCATTTACCCCAGGCGTGCGGGGTCCAAC‑AGATCGACCGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTCACACAGTGc                                               <  7:242285/135‑1 (MQ=255)                                                                             gcgcTTATGACATTGGCCTGCATTTTCCCCAGGCGTGCTTGGTTAAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTCACACAGTGCg                                              <  3:136903/135‑1 (MQ=255)                                                                                 tgatgaCATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTc                                                        <  4:360513/121‑1 (MQ=255)                                                                                 tgatgaCATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTc                                                        >  3:360513/1‑121 (MQ=255)                                                                                 tgatgaCATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTCACACAGTGCGGCCa                                          >  1:183440/1‑135 (MQ=255)                                                                                 tgatgaCATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTCACACAGTGCGGCCa                                          >  7:29917/1‑135 (MQ=255)                                                                                 tgatgaCATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAA‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTCACACAGTGCGGACa                                          >  1:306055/1‑135 (MQ=255)                                                                                         ttGGCCTGAATTTTCCCCCGGGGTGGGTGGGGCAAC‑AGATCGACCGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTCACACAGTGCGGCCATGGTCaaa                                  <  4:5985/135‑1 (MQ=255)                                                                                         tgggCCTGCATTTGCCCCCGGGGTGCGGGGGGCAAC‑ATAGCGACCGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTCACACAGTGCGGCCATGGTCaaa                                  <  6:52733/133‑1 (MQ=255)                                                                                                  aTTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTCACACAGTGCGGCCATGGTCAAAGTgtcgtc                          >  4:355417/1‑134 (MQ=255)                                                                                                  aTTTTCACCAGGCGTGCGTGTGGCAAC‑ACATCGACCGCCGCCGGGTGAAAACCCTGATGAAGAGCGGCCTGATTGGCCAGCGCCATCCGTGCATCAAGCACCTCACACAGTGCGGCCATGGTCAAAGTgtcgtc                          <  6:340430/134‑1 (MQ=255)                                                                                                  aTTTACCCCAGGCGTGCGTGGTTCAAC‑AGATCGAACGCCGCCGGGTGAAAACCATGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTCACACAGTGCGGCCATGGTCAAAGTgtcgt                           <  4:164080/133‑1 (MQ=255)                                                                                                   ttttCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTCACACAGTGCGGCCATGGTCAAAGTGTCGTCAt                        <  8:47755/135‑1 (MQ=255)                                                                                                   ttttCCCAAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCTGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTCACACAGTGCGGCCATGGTCAAAGTGTCGTCAt                        <  8:1367/135‑1 (MQ=255)                                                                                                    tttCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTCACACAGTGCGGCCATGGTCAAAGTgtcgtc                          >  1:95653/1‑132 (MQ=255)                                                                                                        cccTGGCGTGCTGGGTGAAAC‑TGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTCACACAGTGCGGCCATGGTCAAAGTGTCGTCATAGAGc                   <  2:235084/135‑1 (MQ=255)                                                                                                             gcgtgcgtGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTCACACAGTGCGGCCATGGTCAAAGTGTCGTCATAGAGCGTACg              <  2:45264/135‑1 (MQ=255)                                                                                                             gcgggcgtGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTCACACAGTGCGGCCATGGTCAAAGTGTCGTCATAGAGCGTACg              <  8:31346/135‑1 (MQ=255)                                                                                                              cgtgcgtgGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGCGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTCACCCAGTGCGGCCATGGTCAAAGTGTCGTCATAGAGCGTACgg             >  6:258492/1‑135 (MQ=255)                                                                                                              cgtgcgtgGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTCACACAGTGCGGCCATGGTCAAAGTGTCGTCATAGAGCGTACgg             >  8:47919/1‑135 (MQ=255)                                                                                                                   gtggtgCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCAt                                                                  <  4:146307/77‑1 (MQ=255)                                                                                                                   gtggtgCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCAt                                                                  >  3:146307/1‑77 (MQ=255)                                                                                                                   gtggtgCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTCACACAGTGCGGCCATGGTCAAAGTGTCGTCATAGAGCGTACGGATCTg        >  1:275231/1‑135 (MQ=255)                                                                                                                          aaC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTCACACAGTGCGGCCATGGTCAAAGTGTCGTCATAGAGCGTACGGATCTGCTTGAc  >  2:113391/1‑134 (MQ=255)                                                                                                                                     |                                                                                                                            CAGGTACTGATCGTCCTGCACCAGGCACAGCAGATCCTTCAAGGGCGTTTGCGCACCATTGAGGCAAACCAGACCGGCGCTGATGACATTGGCCTGCATTTTCCCCAGGCGTGCGTGGTGCAAC‑AGATCGAACGCCGCCGGGTGAAAACCCTGATGACGAGCGGCCTGATTGGCCAGCGCCAGCCGTGCATCAAGCACCTCACACAGTGCGGCCATGGTCAAAGTGTCGTCATAGAGCGTACGGATCTGCTTGAC  >  ACB122/5241057‑5241311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑