| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 1,047,512 | A→C | 41.2% | intergenic (‑104/+38) | CJ019_00985 ← / ← CJ019_00986 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,047,512 | 0 | A | C | 41.2% | 6.5 / 2.6 | 28 | intergenic (‑104/+38) | CJ019_00985/CJ019_00986 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (14/2); new base C (9/3); total (23/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGC‑AAACGAAAAC‑GCCCGGCACAA‑GGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCA > ACB122/1047385‑1047638 | ccAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGC‑AAACGAAAAC‑CCCCCGCCCAA‑GGcc > 3:225249/1‑135 (MQ=255) cATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGC‑AAACGAAAAC‑GCCCGGCACAA‑GGCCGGGcc > 7:265462/1‑134 (MQ=255) gCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGC‑AAACGAAAAC‑CCCCGGCACAA‑GGGCGGGCGTTTTCCGTGAAGGGCTTccgca > 4:73901/1‑135 (MQ=255) ctcctGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGC‑AAACGAAAAC‑GCCCGGCACAACGGCCGGGC‑TTTGCGGGGAAGCGCAAcagca > 7:303219/1‑133 (MQ=255) ctcctGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGC‑AAACGAAAAC‑GCCCGGCACAA‑GGTCGGGCGTTTGCGGTGAAGCGCTACAGCAgg > 8:288510/1‑135 (MQ=255) ctcctGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGC‑AAACGAAAAC‑GCCCCGCCCAA‑GGCCGGGCGGTTGCCGTGAAGCGCTTCACCAgg > 8:380700/1‑135 (MQ=255) gTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGC‑AAACGAAAAC‑GCCCCGCACAA‑GGCCGGGCGTTTTGGGGGGAGCCCGACAGCACGATCa > 5:196244/1‑134 (MQ=255) gTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGC‑AAACGAAAAC‑GCCCCGCACAA‑GGCCGGGCGGTTGCGGTGAAGCGCTACAGCAGGAtaa > 6:149152/1‑132 (MQ=255) aGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGC‑AAACGAAAAC‑GCCCGGCACAA‑CGCCGGGCGTTTGCGGTGAAGCGCTACAGCCCGATCAGAAc > 3:349887/1‑135 (MQ=255) ggTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGC‑AAACGAAAAC‑GCCCCGCACAA‑GGCCGGGCGTTTGCGGTGCAGCGCGACACCACGATCAGAAca > 1:107592/1‑134 (MQ=255) gTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGC‑AAACGAAAAC‑GCCCCGCCCAA‑GGCCCGGCCTTTTCGGTGAAGCGCTTCAGCAGGAAAAGAAATc > 8:87772/1‑135 (MQ=255) gATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGC‑AAACGAAAAC‑GCCCCGCACAA‑GGCCGGGCGTTTGCGGTGAAGCGCTACAGCCGGCTCAGAACTAGTGa > 6:190308/1‑135 (MQ=255) ccTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGC‑AAACGAAAAC‑GCCCCGCCCAA‑GGCCGGGCGGTTTGGGGGGAGCCCGTCAGCACGCTCAGAACTCGTGTTACGcccgg > 7:335158/1‑131 (MQ=255) ggATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGC‑AAACGAAAAC‑GCCCGGCCCCA‑GGCCGGGCGGTTGCGGGGTAGCGCTACCGCAGGCTCAGAACTCGTGATCCGCAGTGTCCggg > 3:306666/1‑135 (MQ=255) gCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGC‑AAACGAAAAC‑GCCCGGCACAA‑GGGCGGGCGTTTTGGGTGCAGCGCGTCAGCAGGGTCAGCACCCGGGATCCCCAGTGTGCGGGttgacg > 4:271284/1‑131 (MQ=255) gCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGC‑AAACGAAAAC‑GCCCCGCCCAA‑GGCCGGGCGTTTGCGGGGGATCGCGACAGCAGGATCCGAACTCGGGCTCCGCCGGGTGCGGGGTGAgg > 8:57101/1‑135 (MQ=255) gCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGC‑AAACGAAAAC‑GCCCCGCACAA‑GGGCCGGCGTTTTGGGTGGAGCGCTACAGCAGGGTCAGAACTCGGGGTCCGCAGTGTGCGGGTTCAgg > 1:234998/1‑135 (MQ=255) aTCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGC‑AAACGAAAAC‑GCCCCGCCCAA‑GGCCCGGCGTTTGCGGTGAAGCGCTACACCAGGATAAGAACTCGTGATCCCCAGTGTCCGGGGTCAGGtt > 3:254461/1‑135 (MQ=255) gTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGC‑AAACGAAAAC‑GCCCGGCCCAA‑GGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGGGATGCGCAGTGTCCGGGTTCAGGTTGGCAAt > 8:222516/1‑135 (MQ=255) gTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGC‑AAACGAAAAC‑GCCCGGCACAA‑GGCCGGGCGTTTGGGGTGTAGCGCTACAGGAGGGTCAGAACTCGGGCTGCGCCGTGTCCGGGGTCAGGTTGGCAAt > 8:216989/1‑135 (MQ=255) gCCAGGCAGCGGGAAGCGTTTTACCCGGCAGC‑AAACGAAAAC‑GCCCGGCCCAA‑GGCCGGGCGGTTGGGGGGAAGCGCTACAGCAGGATGATCACTCGTGATCCGCAGTGTGCGGGGTCAGGTTGGCAATGccccg > 4:270126/1‑133 (MQ=255) aGCGTTTTACCCGGCAGC‑AAACGAAAAC‑GCCCGGCACAA‑GGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGGTCAGGTTGGGCATGCCCAGCTTTCCCGCAGccg > 5:169092/1‑134 (MQ=255) cccgcccgC‑AAACGAAAAC‑GCCCGGCACAA‑GGCGGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTtcgatc < 4:304812/134‑1 (MQ=255) ccGGCATC‑AAACCCACACGGCCCGGCCCCT‑GGCGGGGGGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATGCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTtcgatcg < 3:284917/135‑1 (MQ=255) ccGGCACCAAAACCACAAC‑GCCCGGCCCAC‑GGCGGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTtcgatcg < 7:311735/135‑1 (MQ=255) ggCAGC‑AAACGAAAAC‑GCCCGGCACAA‑GGTCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGAcc < 7:288510/135‑1 (MQ=255) c‑aAACGAAAAC‑GCCCGGCACAA‑GGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCGGGTCGATCGACCCGGTc > 7:302956/1‑135 (MQ=255) cccccGCCCCAA‑GGCCGGGCGGTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGGTCGATCGACCCGGTCTGCTTGACCa < 7:380700/133‑1 (MQ=255) | CCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGC‑AAACGAAAAC‑GCCCGGCACAA‑GGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCA > ACB122/1047385‑1047638 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |