| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 2,318,786 | A→C | 45.4% | G525G (GGT→GGG) | yjcS ← | Putative alkyl/aryl‑sulfatase YjcS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 2,318,786 | 0 | A | C | 45.4% | 7.6 / 2.4 | 21 | G525G (GGT→GGG) | yjcS | Putative alkyl/aryl‑sulfatase YjcS |
| Reads supporting (aligned to +/- strand): ref base A (2/9); new base C (3/7); total (5/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTTGATGGTCAGGTTGCTATCGGCAGCGTTGTAGGCATTCACCCGAACACCCAGGTAGTCGAAAAACAGGCTGGGTGTCAGGGCCCGCACCATGTCATCGGCG‑TTGCCGCCGCTACCCGCACCGGCGGCAACGCCTGAGC‑GCAATTCCTGCGCGCCACTGA‑GGTAGGCATTGCGCCAGGTGGCGTTCTCACTCTGGTAACCCAGTTGCTCCAGCGCGTCGGCCTGCAGTTCGCGGGCCTCGGTGTTGTCTGGCT > ACB122/2318665‑2318919 | aGTTGATGGTCAGGTTGCTATCGGCAGCGTTGTAGGCATTCACCCGAACACCCAGGTAGTCGAAAAACAGGCTGGGTGTCAGGGCCCGCACCATGTCATCGGCC‑TTTCCCCCCCTACCCCCCCCCGCGGCAACGc > 7:133461/1‑135 (MQ=255) cGGCAGCGTTGTAGGCATTCACCCGAACACCCAGGTAGTCGAAAAACAGGCTGGGTGTCAGGGCCCGCACCATGTCATCGGCG‑TTTCCCCCCCTACCCGCCCCCGCGGCAACGCCAGCGC‑GCAAATGCAgcgcgc > 3:251522/1‑135 (MQ=255) agacaggTTGTAGGCATTCACCCGAACACCCTGGTAGTCGAAAAACCGGCTGGGTGTCTGGGCCCGCACCATGTCAGCGGCG‑TTGCCGCCGCTACCCCCACCGGCGGCAACGCCTGAGC‑GCAATTCCTGCGCGcc < 4:295178/129‑1 (MQ=255) gCAGCGTTGTAGGCATTCACCCGAACACCCAGGTAGTCGAAAAACAGGCTGGGTGTCAGGGCCCGCACCATGTCATCGGCG‑TTGCCGCCGCTACCCGCACCGGCGGAAACGCCTGAGC‑GCAATTCCTGCGCGcc < 3:293868/134‑1 (MQ=255) cggCATCCACCCCAACCCCCTGTGCGGCCAAAAACCGGCGGGGTGCCAGGCCCCGCCCCTTGCCAGCGGCG‑TGGCCGCCGCTACCCCCCCCGGCGGAAACGCCTGAGC‑GCAATTCCTGCGCGCCACTGA‑GGTAg < 8:236868/133‑1 (MQ=255) aGGCATTCACCCGAACACCCAGGTAGTCGAAAAACAGGCTGGGTGTCAGGGCCCGCACCATGTCATCGGCG‑TTGCCGCCGCTACCCGCACCGGCGGAAACGCCTGAGC‑GCAATTCCTGCGCGCCACTGA‑GGTAg < 7:228321/134‑1 (MQ=255) acACCCGAACCCCCAGGTCGTCCAAAAACCGGCGGGGGGTCAGGGCCCCCACCATGTCATCGGCG‑TTGCCGCCGCTACCCGCCCCGGCGGCAACGCCTGAGC‑GCAATTCCTGCGCGCCACTGA‑GGTAGGCATTgc < 1:228119/134‑1 (MQ=255) ggTAGTCGAAAAACAGGCTGGGTGTCAGGGCCCGCACCATGTCATCGGCGTTTGCCG‑CGCGGCCCCCCCCCGCGGCCAAGCCCGCGCGGCAATTCCGGCG‑GCCCCGGAGGGTGGGCATTG‑GCCAGgggggggtgt > 5:378336/1‑129 (MQ=255) ggTAGTCGAAAAACAGGCTGGGTGTCAGGACCCGCACCATGTCATCGGCG‑TTGCCGGCCCGACCCGCACCCGCGGCCACGCCGGCGC‑GGCATTCCAGCGCGCCCCGGA‑GGGAGGCCTTGCGCCCGGTgggggtcg > 7:372518/1‑129 (MQ=255) ggTAGCCCAAAAACCGGCGGGGTGACAGGGCCCGCACCATGTCATCGGCG‑TTGCCGCCGCTACCCGCCCCGGCGGAAACGCCTGAGC‑GCAATTCCTGCGCGCCACTGA‑GGTAGGCATTGCGCCAGGTGGCGTtct < 3:141850/135‑1 (MQ=255) atgTCCAAAACCGGGCGGGGTTTCAGGGGCCCCACCAGGTCTTGGGCG‑GGGCCGCCGAACCCCGCACCGGCGCAAACGCCTGAGC‑GCAATTCCTGCGCGCCACTGA‑GGTAGGCATTGCGCCAGGTGGCGTtct < 1:96369/131‑1 (MQ=255) aaaaaCAGGCTGGGTGTCAGGGCCCGCACCATGTCATCGGCA‑TTGCCGCCGCTACCCGCACCGGCGGCAACGCCTGCGC‑GCAATTCCTGCGCGCCACTGA‑GGTAGGCATTGCGCCAGGTGGCGTTCTCACTCTg > 6:88684/1‑134 (MQ=255) aGGCTGGGTGTCAGGGCCCGCACCATGTCATCGGCG‑TTGCCGCCGCTACCCGCACCGGCGGCAACGCCTGAGC‑GCAATTCCTGCGCGCCACTGA‑GGTAGGCATTGCGCCAGGTGGCGTTCTCACTCTGGTAAccc < 6:345777/135‑1 (MQ=255) gtgtCAGGGCCCGCACCATGTCATCGGCG‑TTGCCGCCGCTACCCGCCCCGGCGGCAACGCCTGAGC‑GCAATTCCTGCGCGCCACTGA‑GGTAGGCATTGCGCCAGGTGGCGTTCTCACTCTGGTAACCCAGTTGCt < 8:69107/135‑1 (MQ=255) ccgcccccCACCATGCCTGGGGGG‑TGGCCGCCGCCACCCCCCCCGCCGCAACCGCCTGAGC‑GCAATTCCTGCGCGCCACTGA‑GGTAGGCATTGCGCCAGGTGGCGTTCTCACTCTGGTAACCCAGTTGCTCCAgc < 1:126869/131‑1 (MQ=255) aTCGGCG‑TGCCCGCCGCTACCCGCACCGGCGGCAACGCCTGAGC‑GCAATTCCTGCGCGCCACTGA‑GGTAGGCATTGCGCCAGGTGGCGTTCTCACTCTGGTAACCCAGTTGCTCCAGCGCGTCGGCCTGCAGTTc < 7:70163/135‑1 (MQ=255) ggCG‑TTGCCGCCGCTACCCGCACCGGCGGCAACGCCTGAGC‑GCAATTCCTGCGCGCCACTGA‑GGTAGGCATTGCGCCAGGTGGCGTTCTCACTCTGGTAACCCAGTTGCTCCAGCGCGTCGGCCTGCAGTTcgcg < 4:165980/135‑1 (MQ=255) cG‑TGGCCGCCGCTACCCGCACCGGCGGCAACGCCTGAGC‑GCAATTCCTGCGCGCCACTGA‑GGTAGGCATTGCGCCAGGTGGCGTTCTCACTCTGGTAACCCAGTTGCTCCAGCGCGTCGGCCTGCAGTTCGCggg < 7:178526/135‑1 (MQ=255) gtgggccgccGCTACCCCCCCGGGCGGAAAGGCCTGAGC‑GCAATTCCTGCGCGCCACTGA‑GGTAGGCATTGCGCCAGGTGGCGTTCTCACTCTGGTAACCCAGTTGCTCCAGCGCGTCGGCCTGCAGTTCGCggg < 7:358099/131‑1 (MQ=255) cgcTACCCGCCCCGGCGGCAACGCCTGAGC‑GCAATTCCTGCGCGCCACTGA‑GGTAGGCATTGCGCCAGGTGGCGTTCTCACTCTGGTAACCCAGTTGCTCCAGCGCGTCGGCCTGCAGTTCGCGGGCCTCGgtgt < 5:152856/135‑1 (MQ=255) cACCGGCGGAAACGCCTGAGC‑GCAATTCCTGCGCGCCACTGA‑GGTAGGCATTGCGCCAGGTGGCGTTCTCACTCTGGTAACCCAGTTGCTCCAGCGCGTCGGCCTGCAGTTCGCGGGCCTCGGTGTTGTCTGGCt < 2:63117/135‑1 (MQ=255) | AGTTGATGGTCAGGTTGCTATCGGCAGCGTTGTAGGCATTCACCCGAACACCCAGGTAGTCGAAAAACAGGCTGGGTGTCAGGGCCCGCACCATGTCATCGGCG‑TTGCCGCCGCTACCCGCACCGGCGGCAACGCCTGAGC‑GCAATTCCTGCGCGCCACTGA‑GGTAGGCATTGCGCCAGGTGGCGTTCTCACTCTGGTAACCCAGTTGCTCCAGCGCGTCGGCCTGCAGTTCGCGGGCCTCGGTGTTGTCTGGCT > ACB122/2318665‑2318919 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |