| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 2,938,227 | T→G | 32.4% | R533R (CGT→CGG) | pqiB → | Intermembrane transport protein PqiB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 2,938,227 | 0 | T | G | 32.4% | 33.3 / 3.9 | 34 | R533R (CGT→CGG) | pqiB | Intermembrane transport protein PqiB |
| Reads supporting (aligned to +/- strand): ref base T (15/7); new base G (3/9); total (18/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.99e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCAACTCGGCCATTTCCGAAGATTCGCCGCAGCGCGAGCGAATGGGTGAAACGCTGGACGAGCTGGAGCGCATGTCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGTCGCTGATTCGTGGCCGGCCGAAAGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAG‑ACAGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGCCTGCTGGTGTGGCTCGGGGGCTGCAGCAGTACGCCGAACAACTACC > ACB122/2938093‑2938359 | gCCAACTCGGCCATTTCCGAAGATTCGCCGCAGCGCGAGCGAATGGGTGAAACGCTGGACGAGCTGGAGCGCATGTCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGTCGCTGATTCGt < 1:124053/135‑1 (MQ=255)gCCAACTCGGCCATTTCCGAAGATTCGCCGCAGCGCGAGCGAATGGGTGAAACGCTGGACGAGCTGGAGCGCATGTCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGTCGCTGATTCGt < 2:336705/135‑1 (MQ=255) cAACTCGGCCATTTCCGAAGATTCGCCGCAGCGCGAGCGAATGGGTGAAACGCTGGACGAGCTGGAGCGCATGTCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGTCGCTGATTCGTgg > 4:175594/1‑135 (MQ=255) cAACTCGGCCATTTCCGAAGATTCGCCGCAGCGCGAGCGAATGGGTGAAACGCTGGACGAGCTGGAGCGCATGTCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGTCGCTGATTCGTgg > 2:86399/1‑135 (MQ=255) cAACTCGGCCATTTCCGAAGATTCGCCGCAGCGCGAGCGAATGGGTGAAACGCTGGACGAGCTGGAGCGCATGTCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGTCGCGGCTTCGTgg > 8:360347/1‑135 (MQ=255) aCTCGGCCATTTCCGAAGATTCGCCGCAGCGCGAGCGAATGGGTGAAACGCTGGACGAGCTGGAGCGCATGTCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGTCGCTGATTCGTggcc < 3:298638/135‑1 (MQ=255) cTCGGCCATTTCCGAAGATTCGCCGCAGCGCGAGCGAATGTGTGAAACGCTGGACGAGCTGGAGCGCATGTCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGTCGCTGAGTCGTggccc > 2:75201/1‑134 (MQ=255) cGGCCATTTCCGAAGATTCGCCGCAGCGCGAGCGAATGGGTGAAACGCTGGACGAGCTGGAGCGCATGTCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCCCCCCGCGTCGCTGAATAGTggccgcc > 6:369173/1‑133 (MQ=255) cGGCCATTTCCGAAGATTCGCCGCAGCGCGAGCGAATGGGTGAAACGCTGGACGAGCTGGAGCGCATGTCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGTCGCTGAGTCGTggccggc > 2:215986/1‑135 (MQ=255) ttCGCCGCAGCGCGAGCGAATGGGTGAAACGCTGGACGAGCTGGAGCGCATGTCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGTCGCTGATTCGTGGCCGGCCGAAAGCGGCCGGGTc > 3:332147/1‑135 (MQ=255) gccgcAGCGCGAGCTAATGGGTGAACCGCTGGCCGAGCTGGCGCGCATGTCGCGTTCGCTACGTGACCTTGCCGATAACCTGGG‑CCGCCACCCCGAGGCGCTGATGCGTGGCCGGCCTAAAGCGGCCGGGTCCg < 4:332147/134‑1 (MQ=255) aGCGCGAGCGAATGGGTGAAACGCTGGACGAGCTGGAGCGCATGTCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGTCGCTGATTCGTGGCCGGCCGAAAGCAGCCGGGTCCGCAGAcc < 8:128570/135‑1 (MQ=255) gaATGGGTGAAACGCTGGACGAGCTGGAGCGCATGTCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGTCGCGGTTTCGGGGCCCGCCGAAAGCGGCCGGGTCCGCCGGCCGTGGAcccg > 1:263442/1‑134 (MQ=255) gTGAAACGCTGGACGAGCTGGAGCGCATGTCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCACCACCCCGAGTCGCTGATTCCGGGCCGGGCGCAAGCGGCCGGCGCCGCAGACCGTGGCCCCTcaggag > 8:341631/1‑135 (MQ=255) aaccgcgGGACGAGGGGGAGCGCATGGCGCGTGCGCTCCGGGCCCTTGCCGATACCCTGGG‑CCGCCCCCCCGAGTCGCTGATGCGTGGCCGGCCGAACGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAG‑ACa < 4:241472/132‑1 (MQ=255) aaaCGCTGGACGAGCTGGAGCGCATGTCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGTCGCTGGTTAGTTGCCCGCCGCAAGCGGGCGGGTGCGCCGGCCGTGGACCCCGAGGAG‑ACa > 6:51854/1‑135 (MQ=255) cGAGCTGGAGCGCATGTCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGTCGCTGATTCGTGGCCGGCCGAAAACGGCCGGGTGCGCAGACCATGAACCCGGAGGAG‑GCACCCGATGaaa > 1:107172/1‑135 (MQ=255) gAGCTGGAGCGCATGTCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGTCGCTGATTCGTGGCCGGCCGAAAGCGGCCCGGTCCGCAGACCATGAACCCGGAGGAG‑CCAGCCGATGCAAc > 1:64745/1‑135 (MQ=255) gAGCTGGAGCGCATGTCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGTCGCGGCTTCGTGGCCGGCCGAAACCGGCCGGGGGCGCCGCCCGTGGACCCCGAGGGG‑ACAGCCGGGGCAAc > 7:161839/1‑135 (MQ=255) tGGCGCGCATGTCGCGTTCGCTACGTGCCCTTGCCGATTACCGGGC‑CCGCCCCCCCGAGGCGCTGATGCGGGGCCGGCCGAACGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAG‑ACAGCCGATGCAACGACt < 2:290569/135‑1 (MQ=255) gggtgcgcAGGTCGCGTTCGCACCGTGACCTTGCGTATTCCGGGGG‑CCGCCACCCCGAGGCGCTGATCCGGGGCCGGCCGAACGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAG‑ACAGCCGATGCAACGACt < 3:269177/131‑1 (MQ=255) gcgcATGTCGCGTTCGCTACGTGACCTTTCCGATTACCTGGG‑CCGCCACCCCGAGTCGCTGATTCGTGGCCGGCCGAAATCGGCCCGGTCCGCAGACCTTGCAACCAGAGGAG‑CCACCCGCTTCAACCACTgcg > 8:201910/1‑134 (MQ=255) tCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGTCGCTGCTTCGTGGCCGGCCGCAAGCGGCCGGGTCCGCAGCCCTTGAACCCGGAGGAG‑CCAGCCGCTGCAACGCCTGCGCAATGCAt > 4:295995/1‑135 (MQ=255) gTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCCCCCCGCGTCGCGGCTTCGGGGCCGGCCGCAAGCCGCCGGGGGCGCCGCCCGTGCACCCGGAGGCGCCCCGCCG‑GGCCACGCCGGCGCCATGCAtgtgt > 5:136785/1‑135 (MQ=255) ccTTGCCGATTCCCGGGC‑CCGCCACCCCGCGGCGCTGATGCGGGGCCGGCCGAACGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAG‑ACAGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGCctgctg < 2:18737/135‑1 (MQ=255) tACCCGGGCCCGCCACCCCGCGGCGCTGATGGGGGGCCGGCCGCACGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAG‑ACAGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGCCTGCTGGTGTGGCTc < 8:52898/135‑1 (MQ=255) aaCCTGGC‑CCCCCACCCCGATGCCCTGATGCGTGCCCGCCCGAACGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAG‑ACAGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGCCTGCTGGTGTGGCTc < 6:257960/133‑1 (MQ=255) gggg‑CCGCCCCCCCGCGGCGCTGATGCGGGCCCGCCCTAAAGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAG‑ACAGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGCCTGCTGGTGTGGCTCggggg < 6:176613/134‑1 (MQ=255) gggg‑CCCCCCCCCCGCGTCCCGGATAGGGGGCCGCCCGACCGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAG‑ACAGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGCCTGCTGGTGTGGCTCggggg < 4:234125/134‑1 (MQ=255) gggg‑CCCCCCCCCCGCGGCGCTGATGCGGGGCCGGCCGAACGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAG‑ACAGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGCCTGCTGGTGTGGCTCggggg < 5:193340/134‑1 (MQ=255) ccccccGCGCCGCTGAGGCGGGGCCGGCCGAACGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAG‑ACCGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGCCTGCTGGTGTGGCTCGGGGGCTgcagca < 4:33537/133‑1 (MQ=255) ccccGAGTCGCTGATTCGTGGCCGGCCGAAAGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAG‑ACAGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGCCTGCTGGTGTGGCTCGGGGGCTACAGCAGt > 1:201325/1‑135 (MQ=255) ggcggtGATGCGGGGCCGGCCGAACGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAG‑ACAGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGCCTGCTGGTGTGGCTCGGGGGCTGCAGCAGTACGCCg < 7:360347/130‑1 (MQ=255) gTCGCTGATTCGTGGCCGGCCGAAAGCGGCCGGGTCCGCACACCTTTAACCCGGACGAG‑ACAGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGCCTGCTGGTGTGGCTCGGGGGCTGCCGCAGTACGcc > 4:290535/1‑134 (MQ=255) ttCGTGGCCGGCCTAAAGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAG‑ACAGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGCCTGCTGGTGTGGCTCGGGGGCTGCAGCAGTACGCCGAACAACTa < 3:175594/135‑1 (MQ=255) gTGGCCGGCCGAAAGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAG‑ACAGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGCCTGCTGGTGTGGCTCGGGGGCTGCAGCAGTACGCCGAACAACTAcc < 8:202247/134‑1 (MQ=255) | GCCAACTCGGCCATTTCCGAAGATTCGCCGCAGCGCGAGCGAATGGGTGAAACGCTGGACGAGCTGGAGCGCATGTCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGTCGCTGATTCGTGGCCGGCCGAAAGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAG‑ACAGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGCCTGCTGGTGTGGCTCGGGGGCTGCAGCAGTACGCCGAACAACTACC > ACB122/2938093‑2938359 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |