Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,312,431 T→G 28.1% T258P (ACG→CCG)  cynR_3 ← HTH‑type transcriptional regulator CynR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,312,4310TG28.1% 33.7 / 3.3 36T258P (ACG→CCG) cynR_3HTH‑type transcriptional regulator CynR
Reads supporting (aligned to +/- strand):  ref base T (17/7);  new base G (5/6);  total (23/13)
Fisher's exact test for biased strand distribution p-value = 2.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGACAACTGCTGCGGTGCCAGAAACGCATCATGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGC‑TGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTT  >  ACB122/3312297‑3312562
                                                                                                                                       |                                                                                                                                   
cGACAACTGCTGCGGTGCCAGAAACGCATCATGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCACCCCCACGCTCATCGGCGTGACCGGGTCGC‑TGATGGTCACCGt                                                                                                                                     >  2:81888/1‑135 (MQ=255)
     aCTGCTGCGGTGCCAGAAACGCATCATGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGC‑TGATGGTCACCGTCTCGa                                                                                                                                >  3:280258/1‑135 (MQ=255)
            cGGTGCCAGAAACGCATCATGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCGTGATGGTCACCGTCTCGATGCGCt                                                                                                                          >  4:274523/1‑135 (MQ=255)
                 ccAGAAACGCATCATGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGC‑TGATGGTCACCGTCTCGATGCGCTTGCCCt                                                                                                                    >  4:149309/1‑135 (MQ=255)
                 ccAGAAACGCATCATGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGC‑TGATGGTCACCGTCTCGATGCGCGTGCCCt                                                                                                                    >  3:285779/1‑135 (MQ=255)
                                  aaGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGC‑TGATGGTCACCGTCGCGATGCGCTTGCCCGCCACCGACCACGGCCgg                                                                                                   >  8:156212/1‑135 (MQ=255)
                                  aaGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGC‑TGATGGTCACCGTCGAGATGAGCTTTCCCTCCAGCCCCCAccgccgc                                                                                                   >  6:302334/1‑134 (MQ=255)
                                        ttGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCCCGCTCATCGGCGTGACCGGGTCGC‑TGATGGTCCCCGTCTCGCTGCGCTTGCCCGCCCGCGACCCCGGCCGGTGcccc                                                                                             >  4:218261/1‑135 (MQ=255)
                                        ttGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGC‑TGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGGAcc                                                                                             >  3:263125/1‑135 (MQ=255)
                                        ttGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGC‑TGATGGTCACCGACTAGATTCGCATGCCCTCCAGCGACCACCGCCCGTGCAcc                                                                                             >  3:307034/1‑135 (MQ=255)
                                           cccACCGCCTGCATCGCCGGGCTGAAGGCCCGTCCCCGGGCCCAGGCCACCCCCACGCTCCTCGGCGTGACCGGGGCGC‑TGAGGGGCACCGTCTCGATGCGCTTGCCCCCCCGCGCCCACGGCCGGTGCACCAgg                                                                                          <  6:11070/133‑1 (MQ=255)
                                                cGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGC‑TGATGGTCACCGTCGCGATGCGCTTGCCCTCCAGCGCCCACGGCCGGGGCACCCGGTCCgc                                                                                     >  6:257124/1‑134 (MQ=255)
                                                   cTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGC‑TGATGGTCCCCGGCTCGCTGCGCTTGCCCGCCCGCGCCCACCGCCCGTGCCCCAGGGCCGACAg                                                                                  >  2:365691/1‑135 (MQ=255)
                                                             gggCTGAATGCCCGTTCCCGGTGCCAGGCAAACCACACGCTAATCGGCGTGACCGGGTCGC‑TGATGGTCCCCGTCTCGATGCGCTTGCCCTCCAGCGCCCACGGCCGGTGCCCCAGGTCCGCCAGGATCGCCCCt                                                                        >  8:303562/1‑135 (MQ=255)
                                                             gggCTGAATGCCCGTGCCCGGGCCCAGCCCAACCCCACGCGCAGCGGCGTGACGGGGTCGC‑TGATGGTCACCGGCTCGAGGCGCTTGCCCTCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACt                                                                        <  3:28937/135‑1 (MQ=255)
                                                               gCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGC‑TGATGGTGCCCGGCGCGATGCGCTTGCCCTCCAGCGCCCCCGGCCGGGGCACCAGGTCCGACCGGATCGCCACTcc                                                                      >  5:20078/1‑135 (MQ=255)
                                                                      gCCCGTGCCCGGGGCCAGGCCAACCCCACGCTCATCGGCGTGACGGGGTCGC‑TGATGGTCACCGGCTCGATGCGCTGGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGcc                                                                <  7:279020/134‑1 (MQ=255)
                                                                        ccGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGC‑TGATGGTCACCGTCTCGCTGCGCGTGCCCGCCCCCGCCCCCCGCCGGTGCACCAGGTCCGACCCGATCGCCCCGCCGCTGCCagt                                                             >  7:318093/1‑133 (MQ=255)
                                                                          gtccccGGTGCCCGGCCAACCCCCCGCTCAGCGGCGTGACCGGGTCGC‑TGATGGGCACCGGCTCGAGGCGCTGGCCCCCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTgg                                                           <  3:90267/132‑1 (MQ=255)
                                                                             cccGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGC‑TGATGGTCACCGTCTCGCTGCGCTTGCCCGCCAGCGACCACGGCCGGTGCACCCGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCgc                                                        >  3:306786/1‑134 (MQ=255)
                                                                               cGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGC‑TGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGAcc                                                      <  4:280258/135‑1 (MQ=255)
                                                                                ggTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGC‑TGATGGTCACCGGCTCGCTGCGCGTGCCCGCCCCCGCCCCCGGCCGGGGCCCCCGGTCCGACAGGATCGCGCCTCCGCAGCCCGTGGCGcccc                                                     >  5:15649/1‑134 (MQ=255)
                                                                                     cAGCCCAACCCCACGCTCATCGGCGTGACCGGGTCGC‑GGAGGGTCCCCGTCTCGATGCGCTGGCCCTCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCAtgctg                                                <  4:285779/135‑1 (MQ=255)
                                                                                               ccACGCTCATCGGCGTGACCGGGTCGC‑TGATGGTCACCGTCTCGATGCGCTTTCCCTCCAGCGCCCACGGCCGGAGCCCCAGGTCCGACAGGATAGCCACTCCGCTGCCATTTGCGCCCAAGCTTCGCACGGCCt                                      >  7:168141/1‑135 (MQ=255)
                                                                                               ccACGCTCATCGGCGTGACCGGGTCGC‑GGATTGTCACCGGCTCGCTGCGCTTGCCCTCCAGCGCCCACCGCCGGGGCCCCCCGGCCGCCAGGATCGCCCCGCCCCTGCCCTTGGCGCCCAAGCTGCGCCCGGCCt                                      >  2:157053/1‑135 (MQ=255)
                                                                                                     tCATCGGCGTGACCGGGTCGC‑TGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCAc                                  >  8:115093/1‑133 (MQ=255)
                                                                                                     tCATCGGCGTGACCGGGTCGC‑TGATGGTCACCCTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCCCACGGTCCGACAGCAACGCCACTCCGCTGCCCTTGGCGACCATGCTGCGCACGGCcaccac                                  >  7:89861/1‑133 (MQ=255)
                                                                                                          ggCGTGACCGGGTCGC‑TGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGCCAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCCCGGCCTCCACCGCGCTg                           >  1:22936/1‑135 (MQ=255)
                                                                                                          ggCGTGACCGGGTCGC‑TGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTACGACAGGATCGCCACTACGCTGCCATT‑GCGACCATGCTTCGCACGGCCTCCCCCGAGCggg                          >  5:131599/1‑135 (MQ=255)
                                                                                                          ggCGTGACCGGGTCGC‑TGATGGTCACCGGCTCGATGCGCTTGCCCTCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACGCCCCTGCCATTGGCGACCATGCTGCGCACCGCCGCCACCGAGCTg                           >  6:348448/1‑135 (MQ=255)
                                                                                                            ggTGACGGGGGCGG‑TGTTGGGCACCGTCTCGATGCGGTTGCCCCCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                         <  8:168141/134‑1 (MQ=255)
                                                                                                            cgggACCGGGTCGC‑TGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                         <  8:127292/132‑1 (MQ=255)
                                                                                                            cGTGCCCGGGGCGC‑TGATGGGCACGGGCTCGAGGCGCTGGCCCTCCCGCGCCCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                         <  3:218261/135‑1 (MQ=255)
                                                                                                            cGTGACCGGGTCGC‑TGATGGCCACGGGCTCGATGCGCTGGCCCTCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                         <  3:136701/135‑1 (MQ=255)
                                                                                                            cGTGACCGGGGCGC‑TGATGGCCCCGGGCTCGATGCGCTTGCCCGCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                         <  3:11581/135‑1 (MQ=255)
                                                                                                            cGTCACCGGGTCGC‑GGAGGGGCCCCGTCTCGAGGCGCTTGCCCTCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                         <  7:28986/135‑1 (MQ=255)
                                                                                                                                    cggTCTGGAGGCGCTTCCCCTCCGGCCTCCACGGCCGGTGCACGAGGTGCGACAGTATCGCCACTCCGCTGCCATTGGCGACGATGCTGCGCACGGCCTCCACCGAGCTGGTTCGCACCCTTACTCTTGGCCGtt  <  8:128146/133‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                   
CGACAACTGCTGCGGTGCCAGAAACGCATCATGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGC‑TGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTT  >  ACB122/3312297‑3312562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑