Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	4,077,164	T→G	34.9%	L173R (CTG→CGG)	artJ_2 →	ABC transporter arginine‑binding protein 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	4,077,164	0	T	G	34.9%	21.9 / 3.9	43	L173R (CTG→CGG)	artJ_2	ABC transporter arginine‑binding protein 1
Reads supporting (aligned to +/- strand):  ref base T (18/6);  new base G (11/2);  total (35/8)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCGCAGTCTTCCTCTACCCAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CTGGACGGCGTGATTGCCG‑ACAAATTCCCGCTGCACGAGTGGATGAACAAGAACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGACACCAAGGCCGATGCCGCCATCGCTGTGCGCAAGGACGACGAA  >  ACB122/4077044‑4077297                                                                                                                          |                                                                                                                                       gTGCGCAGTCTTCCTCTACCCAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CTGGACGGCGTGATTg                                                                                                                          >  2:127277/1‑135 (MQ=255) gTGCGCAGTCTTCCTCTACCCAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CGGGGCGGCGTTATTg                                                                                                                          >  6:378015/1‑135 (MQ=255) gTGCGCAGTCTTCCTCTACCCAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CAGGACGGCGGGCTTg                                                                                                                          >  3:45611/1‑135 (MQ=255)   gcgcAGTCTTCCTCTACCCAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CTGGCCGGCGTGATTGcc                                                                                                                        >  3:180312/1‑135 (MQ=255)   gcgcAGTCTTCCTCTACCCAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CTGGACGGCGTGATTGcc                                                                                                                        >  8:282011/1‑135 (MQ=255)         tctcCCTCTACCCCGCCCATCAACCCCTAAGCCGTGTCCGCCACGGCCGGGGCCGACGTGAAACTGTACCCGACCATGGACGACGCCAACGCAGACCTGGCGGCCGGCCGC‑CTGGACGGCGTGATTGCCG‑ACa                                                                                                                   <  2:63223/133‑1 (MQ=255)                      aGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGCGCTGGACGGCGTGATTGCCG‑ACAAATTCCCGCTGCAc                                                                                                     >  1:63223/1‑135 (MQ=255)                      aGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CTGGCCGGCGTGATTGCCG‑ACAAATTACCGCGGCa                                                                                                      >  7:23718/1‑133 (MQ=255)                      aGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CTGGACCGCGTGCTTGCCG‑CCAAATTCCCGCTGcc                                                                                                      >  3:309709/1‑132 (MQ=255)                      aGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CGGGGCGGCGGGCTTGCCG‑CCCAATTACCGCGGCa                                                                                                      >  5:129342/1‑133 (MQ=255)                      aGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CGGGACGGCGGGATTGCCGCCCAAATACCCgggcc                                                                                                       >  3:335627/1‑129 (MQ=255)                      aGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CAGGACGGCGGGATTGGCG‑ACAAATTACCGCTg                                                                                                        >  1:18572/1‑131 (MQ=255)                      aGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCCC‑CGGGCCGGCGTGTGTTCCG‑CCAAATTCCCGCGGCa                                                                                                      >  3:164989/1‑133 (MQ=255)                      aGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCCC‑CGGGCCGGCGGGGTTGGCG‑CCAAAATCCCGCGGCAcc                                                                                                    >  1:182368/1‑134 (MQ=255)                      aGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCCC‑CGGGCCCGCGGGGATGCCG‑CCAAATTCCCGCGGcc                                                                                                      >  7:275077/1‑132 (MQ=255)                      aGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCCC‑CCGGACCGCCTGTGTGGCG‑AACAAATACCGCAGCAcc                                                                                                    >  7:23716/1‑134 (MQ=255)                        gCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCCC‑CAGGCCGGCGTGATTGCCG‑ACCAAATACCCCTGCACCAg                                                                                                  >  2:82924/1‑135 (MQ=255)                           atcaccGCCGAAGCCGGTTCCGGCATGGCGGGGGCCGAGTTCAAACTGTACCCGACCTTCAACGAAGCCACCGCAGCCCGGGCGGCCGGCCGC‑CTGGACGGCGTGATTGCCG‑ACAAATTCCCGCTGCACGAGTgg                                                                                               <  8:270327/130‑1 (MQ=255)                           aTCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGG‑CTGGACGGCGTGATTGCCG‑CCAAATTACCGCGGCCCGAgg                                                                                                 >  7:167011/1‑132 (MQ=255)                           aTCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CTGGCCGGCGTGATTGCCG‑CCAAATTACCGCTGCACGACTgg                                                                                               >  3:314301/1‑135 (MQ=255)                           aTCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CTGGCCGGCGTGATTGCCG‑ACAAATTCCCGCTGCACGAGTgg                                                                                               >  4:172271/1‑135 (MQ=255)                           aTCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CTGGACGGCGTGATTGCCG‑ACAAATTCCCGCTGCACGAGTgg                                                                                               >  4:25270/1‑135 (MQ=255)                                cGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTCCCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCCG‑CGGGCCGGCGTGATTGCCG‑CCAAATTACCGCAGCCCCCGTggagga                                                                                           >  7:189633/1‑134 (MQ=255)                                cGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CTGGACGGCGTGATTGCCG‑ACAAATTCCCGCTGCACGAGTGGATGa                                                                                           >  4:298440/1‑134 (MQ=255)                                         cGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCCC‑CCGGACCGCGTGATTGCCG‑CCAAATTCCCGCCGCACGAGGGGGAGCACAAGAAcgg                                                                                 >  1:221265/1‑135 (MQ=255)                                                    tggCCGGGGCCGACGTGAACCTGAACCCCACCAGGCCCGAAGCCAACGCAGCCCGGGCGGCCGGCCGC‑CGGGACGGCGTGATTGCCG‑ACAAATTCCCGCTGCACGAGTGGATGAACAAGAACGGCGGGGATtgct                                                                      <  3:279838/134‑1 (MQ=255)                                                        cGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CTGGACGGCGTGATTGCCG‑CCAAATTCCCGCTGCACGAGTGGATGAACAAGAACGGCGGGGATtgctg                                                                     >  4:40942/1‑132 (MQ=255)                                                        cGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CGGGTCGGCGTGGTTTGCG‑ACAAATTCCCGGTGCACGCGTGGTTGTACAAGAACGGCGGGGGTtgctg                                                                     >  6:343225/1‑132 (MQ=255)                                                        cGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CGGGGCGGCGGGATTGGCG‑CCAAAATGCCGCGGCCCGCGTGGTTGAACAAGAACGGCGGGGCTTGCTGCaa                                                                  >  1:145841/1‑135 (MQ=255)                                                        cGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CGGGACGGCGTGATTGCCG‑ACAAATTACCGCTGCACGCGTGGATGTACAAGAACGGCGGGGATtgctg                                                                     >  6:118962/1‑132 (MQ=255)                                                          gTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CTGGACGGCGTGATTGCCG‑ACAAATTCCCGCTGCACGAGTGGATGAACAAGAACGGCGGGGATtgctg                                                                     >  8:114235/1‑130 (MQ=255)                                                             ccGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CTGGACGGCGTGATTGCCG‑ACAAATTCCCGCTGCACGAGTGGATGAACAAGAACGGCGGGGATTGCTGCAAGATcc                                                             >  4:236549/1‑135 (MQ=255)                                                                        cTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CTGGCCGGCGTGATTTCCG‑ACAAATTCCCGCTGCACGAGTGGATGAACAAGAACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTg                                                  >  2:289128/1‑135 (MQ=255)                                                                              ccGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CTGGACGGCGTGATTGCCG‑ACAAATTCCCGCTGCACGAGTGGATGAACAAGAACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGac                                            >  8:262511/1‑135 (MQ=255)                                                                              ccGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CCGGACCGCGGGGTTGCCG‑ACAAAATCCCCCAGCACGTCTGGTTGGACAAGCAAGGCGGGGGTTGGGGCAAGAGACTTTGGGACGTGGCggcc                                            >  5:248667/1‑133 (MQ=255)                                                                                   cATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CTGGCCGGCGTGATTGCCG‑ACAAATTACCGCTGCACGCGTGGATGAACAAGAACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGCCCCCaa                                       >  7:197432/1‑135 (MQ=255)                                                                                   cATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CGGGCCCGCGTGATTGCCG‑ACAAATTACCGCTGCACCAGTGGCTGTACAAGAACCGCGGGGCTTGGTGCAAGATACTTGGCGCCGTGGCGGCCAccca                                       >  7:9281/1‑133 (MQ=255)                                                                                                 acccAGCCCGGGCGGCCGGCCGC‑CTGGACGGCGTGATTGCCG‑ACAAATTCCCGCTGCACGAGTGGATGAACAAGAACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGACACCAAGGCCGATGCCGCCa                         <  5:159923/132‑1 (MQ=255)                                                                                                          tGGCGGCCGGCCGC‑CTGGACGGCGTGATTGCCG‑ACAAATTCCCGCTGCACGAGTGGATGAACAAGAACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGCCACCAAGGCCGATGCCGCCATCGCTGTgc                >  8:285025/1‑135 (MQ=255)                                                                                                            gcggcCGGCCGC‑CTGGACGGCGTGAGTGCCG‑ACAAATTCCCGCTGCACGAGTGGATGAACAAGAACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGACACCAAGGCCGATGCCGCCATCGCTGTgcgc              <  3:241933/135‑1 (MQ=255)                                                                                                              gggcggcccC‑CTGGACGGCGTGATTGCCG‑ACAAATTCCCGCTGCACGAGTGGATGAACAAGCACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGACACCAAGGCCGATGCCGCCATCGCTGTGCGCaa            <  1:3994/132‑1 (MQ=255)                                                                                                              ggccggccGC‑CGGGACGGCGTGATTGCCG‑ACAAATTCCCGCTGCACGAGTGGATGAACAAGCACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGACACCAAGGCCGATGCCGCCATCGCTGTGCGCaa            <  1:127277/135‑1 (MQ=255)                                                                                                              gccgggccgC‑CTGGACGGCGTGATTGCCG‑ACAAATTCCCGCGGCACGCTTGGAGGAACTCGACCGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGACACCAAGGCCGATGCCGCCATCGCTGTGCGCaa            <  6:118108/131‑1 (MQ=255)                                                                                                                         gtGGACGGCGTTATGGCCG‑ACAACTACCCGCTGCACGTGGGGATGAACAAGAACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGACACCAAGGCCGATGCCGCCATCGCTGTGCGCAAGGACGACGaa  <  3:282806/134‑1 (MQ=255)                                                                                                                          |                                                                                                                                       GTGCGCAGTCTTCCTCTACCCAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGCCGC‑CTGGACGGCGTGATTGCCG‑ACAAATTCCCGCTGCACGAGTGGATGAACAAGAACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGACACCAAGGCCGATGCCGCCATCGCTGTGCGCAAGGACGACGAA  >  ACB122/4077044‑4077297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑