Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 4,688,819 G→A 7.9% R277H (CGC→CAC)  yhaJ_2 → HTH‑type transcriptional regulator YhaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,688,8190GA 7.9% 114.4 / 2.1 50R277H (CGC→CAC) yhaJ_2HTH‑type transcriptional regulator YhaJ
Reads supporting (aligned to +/- strand):  ref base G (20/26);  new base A (2/2);  total (22/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GGGCTGGGCTGGGCCTACCTGCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGCCCAGCCCTTTGGGCTG  >  ACB122/4688686‑4688948
                                                                                                                                      |                                                                                                                                   
gggctgggctGGGCCTACCTGCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTcgcgg                                                                                                                                    >  7:149275/1‑134 (MQ=255)
    tgggctgggcCTACCTGCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGt                                                                                                                                >  3:84552/1‑135 (MQ=255)
         tgggcCTACCTGCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGTTGGGc                                                                                                                           >  1:317861/1‑135 (MQ=255)
         tgggcCTACCTGCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGTTGGGc                                                                                                                           >  8:247233/1‑135 (MQ=255)
         tgggcCTACCTGCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGTTGGGc                                                                                                                           >  3:161764/1‑135 (MQ=255)
          gggcCTACCTGCCGCAGCCATTGGTCGCACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGTAGGGcc                                                                                                                          >  3:266239/1‑135 (MQ=255)
          gggcCTACCTGCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGGCCGTTGGGcc                                                                                                                          >  2:118035/1‑135 (MQ=255)
              cTACCTGCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGGTGGGCCTggg                                                                                                                      >  7:37421/1‑135 (MQ=255)
                   tGCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGGCCCGTGGGCCGGGgggcgc                                                                                                                 >  7:81779/1‑135 (MQ=255)
                   tGCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGTTGGGCCGGGgcgcgc                                                                                                                 >  3:264362/1‑135 (MQ=255)
                   tGCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGGTGGGCCTGGgcgcgc                                                                                                                 >  7:260703/1‑135 (MQ=255)
                     ccGCAGCCATGGGTCGAACCGCTGATTGCCGCGGGCGCACTGGCCGAGGTGCGCTTCGACACCATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGGCGCGCCCGTTGGGCCTGGGCGCGCGa                                                                                                               <  7:315424/135‑1 (MQ=255)
                        cAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGGTGGGCCGGGGCGCGCGgcgc                                                                                                            >  3:267561/1‑135 (MQ=255)
                        cAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCCGTGGGGCGGGGCGCGcgccgc                                                                                                            >  3:272562/1‑135 (MQ=255)
                        cAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCACCCGTTGGGCCTGGGCGCGCGACg                                                                                                             >  6:271090/1‑134 (MQ=255)
                        cAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCACCCGTTGGGCCTGGGCGCGCGACg                                                                                                             >  6:275857/1‑134 (MQ=255)
                         aGCCATTGGGCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGGGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGACCAAGTCGCGCCCGTTGGGCCTGGGCGCGCGACGCt                                                                                                           <  8:321130/135‑1 (MQ=255)
                              ttGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCGTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGTTTGGCCGGGGGGCGCGCCGCGACCTg                                                                                                      >  1:208414/1‑135 (MQ=255)
                                 gtctacccGCTGATTGCCTCGGGCGCAGTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGAAAAAGCCGCGCCCGTGGGGCCTGGGCGCGCGACGCTACCTGGa                                                                                                    <  4:371846/128‑1 (MQ=255)
                                                     gggCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGGCCGTTGGGCCTGGGCGCCCGGCGGGACCCGGAAGTCATG‑CGCCCGCTGtt                                                                                 >  2:6707/1‑132 (MQ=255)
                                                     gggCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGTTGGGCCTGGGCGCGCGCCGCTCCCTGGAAGTCATG‑CGCCAGCTGtt                                                                                 >  4:57052/1‑132 (MQ=255)
                                                         gcACTGGCCGCGGTGCGCTTCGAAAACATGGCCAACCGCCTGCGGTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAg                                                                          <  4:250747/135‑1 (MQ=255)
                                                          cagtGGCCGTGGGGGGGTTCTACAACTTGGCAAACCGCGTGGGCTGGGGGT‑TAGACATCCTGTGTATCAGGGCGCGCCGGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAgg                                                                         <  4:267561/132‑1 (MQ=255)
                                                          cACTGGCGGTGGGGCGCTTCGAAAACAGGGCCAACCGCCGGCGGTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCGGTTGGGCCGGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAgg                                                                         <  6:381583/135‑1 (MQ=255)
                                                          cACGGGCGGTGGTGCGCTTCGACAAC‑TGGCCCACCCGCGGGGGTTGGGGGTTATACATCTTGTGGATCACGTCGCGCCGGTGGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAgg                                                                         <  4:272562/135‑1 (MQ=255)
                                                              ggcggtggTGGGCTTCGACACCCTGGCCAACCGCATGCGCTTGGGGG‑TCGAAATCATCGGCAGCAAGGCGCGCCCGTTGGGCCGGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑cc                                                                    <  8:354196/129‑1 (MQ=255)
                                                               gtcgtggtgcggtgcGACAACCTGCCCAACCGCCGGCGCGTGTGGC‑TCGACCTCATGTGTATCTTGTCGGGCCCGTTGGGGCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑ccc                                                                   <  6:60235/130‑1 (MQ=255)
                                                                  gtggTGCGCGTCCACAACATGCCCAACCGCGGGCGCTGGGGGG‑TCCACATCATCTGGATCACGGCGCGCCCGTGGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑cccccc                                                                <  7:177001/133‑1 (MQ=255)
                                                                  gAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGGCCGTTGGGCCTGGGCGCGCGCCGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGCAGGAG‑cccccc                                                                >  7:59537/1‑135 (MQ=255)
                                                                  gAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGTTGGGGCTGGGCGCGCGCCGCGACCTGGAAGTCATG‑CGCCAGCTGTCGGAGAAGGCG‑cccccc                                                                >  3:319025/1‑135 (MQ=255)
                                                                         gctggaacaactGGGCCAACCGCCGGGGGTTTGGGT‑TACAAATCATCTGAACCAAGGCGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCt                                                         <  6:347627/129‑1 (MQ=255)
                                                                             cGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGTTGGGCCTGGGCGCGCGCCGCGACCTGGAAGTCCTG‑CGGCCGCGGGTCGAGAAGGCG‑CCCCCCCCGGCCGGat                                                      >  4:350036/1‑134 (MQ=255)
                                                                                   cttGGCCAACCGCCGGCGCTTGTGGG‑TACACTCCATCTGGAGCACGGCGCGCCCGTTGGGCGGGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCtt                                               <  7:155266/133‑1 (MQ=255)
                                                                                   ctgggCCACCCGCCGCCGCTTGGGGG‑TAGACATCTGTTGGAGAACGCCGCGCCCGTTGGGCCGGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCtt                                               <  1:362870/132‑1 (MQ=255)
                                                                                   caTGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCTTCTGGATCAAGGCGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCtt                                               <  4:99023/135‑1 (MQ=255)
                                                                                   caTGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGTTGGGCCTGGGCGCGCGCCGCTACCGGGAAGTCATG‑CGCCAGCGGTTCGAGAAGGAG‑CCCCCCAGGGCCGGATATCCCtt                                               >  8:308382/1‑135 (MQ=255)
                                                                                   caTGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGTGGGGCCGGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCtt                                               <  3:185870/135‑1 (MQ=255)
                                                                                   caTGGCCAACCGCCTGCGCTTGTGGG‑TAGAAATCAGCTGGATCACGCCGCGCCCGTTGGGCCGGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCtt                                               <  2:56516/135‑1 (MQ=255)
                                                                                    aggcccAACCGCCTGCGCTTGTGGG‑TAGACACCATCTGGAGCAAGTCGCGCCCGTGGGGCCGGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTg                                              <  3:116230/131‑1 (MQ=255)
                                                                                           cccGCCTGCGCTTGTGGG‑TAGCCATCATCTGCATCAGGTCGCGCCCGTGGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGt                                       <  5:103567/134‑1 (MQ=255)
                                                                                             cGCCTGCGCTTGTGGC‑TACACATCATCTGGATCAAGACGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTcc                                     <  1:248607/135‑1 (MQ=255)
                                                                                                                 aCATCTTCTGTACCAAGTCGCGCCGGTTGGGCGTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGc                  <  2:208414/135‑1 (MQ=255)
                                                                                                                 aCATCATCTGGATCATGCGGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGc                  <  2:317861/135‑1 (MQ=255)
                                                                                                                 aCATCATCTGGATCAAGGCGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGc                  <  2:77997/135‑1 (MQ=255)
                                                                                                                 aCATCATCTGGATCAAGCCGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGc                  <  4:102677/135‑1 (MQ=255)
                                                                                                                 aCATCATCTGGATCAACTCGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGc                  <  4:161764/135‑1 (MQ=255)
                                                                                                                 aCATAATCGGCATCACGCCGCGCCCGTGGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGc                  <  8:167892/135‑1 (MQ=255)
                                                                                                                   atcatcTGGATCAAGTCGCGCCCCTTGGGCCTGGGGGCCCGCCGCGACCGGGGACTGATGCCGCCA‑CTGGTGGACAAAGAGCCCCCCCCGGGCCGGTTA‑ACCGTGCCCCCGGCCCCGCCCAGCCGGCGTTCGccc                >  8:176040/1‑135 (MQ=255)
                                                                                                                           gATCCAGGCGCACCCGGTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGCCCAGCCCttt        <  5:271090/135‑1 (MQ=255)
                                                                                                                            aTCAAGTCGCACCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGCCCAGCCCttt        <  5:275857/134‑1 (MQ=255)
                                                                                                                                 ggcgcgCCCGTTGGGCCGGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGCCCAGCCCTTTGGGCtg  <  3:350036/133‑1 (MQ=255)
                                                                                                                                 gccgcgCCCGTTGGGCCGGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGCCCAGCCCTTTGGGCtg  <  1:118035/133‑1 (MQ=255)
                                                                                                                                 gTCGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGCCCAGCCCTTTGGGCtg  <  8:37421/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                   
GGGCTGGGCTGGGCCTACCTGCCGCAGCCATTGGTCGAACCGCTGATTGCCTCGGGCGCACTGGCCGAGGTGCGCTTCGACAACATGGCCAACCGCCTGCGCTTGTGGG‑TAGACATCATCTGGATCAAGTCGCGCCCGTTGGGCCTGGGCGCGCGACGCTACCTGGAAGTCATG‑CGCCAGCTGTTCGAGAAGGAG‑CCCCCCAGGGCCTGATATCCCTTGCACGCGTCCAGGCCCTGCAGGCGTTCGCCCAGCCCTTTGGGCTG  >  ACB122/4688686‑4688948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑