Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,791,6780TG32.6% 32.0 / 11.8 47L182R (CTG→CGG) lpxBLipid‑A‑disaccharide synthase
Reads supporting (aligned to +/- strand):  ref base T (23/8);  new base G (5/10);  total (29/18)
Fisher's exact test for biased strand distribution p-value = 1.13e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.50e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GACCTGATGCTGACGCTGCTGCCGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTCGTGTTG  >  ACB122/1791545‑1791811
                                                                                                                                      |                                                                                                                                     
gtccTGATGCTGACGCTGCTGCCGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CCGCGCGTGCCGCGctgg                                                                                                                                     <  7:245089/134‑1 (MQ=255)
               ctgctgCCGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGc                                                                                                                      >  4:83177/1‑136 (MQ=255)
                           gAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGCCACCATCCCGCTTGAAGCCGACCGCCCGG‑CCGCGCGGGCCGCGCTGGGCCTGGGCGCGGGGCCGGtggtgggg                                                                                                           >  1:191759/1‑133 (MQ=255)
                               cgcgGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGAt                                                                                                      >  4:120113/1‑136 (MQ=255)
                               cgcgGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGAt                                                                                                      >  4:114583/1‑136 (MQ=255)
                                  ggTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CCGCGCGGTCCGCGCGGGGCCTGGGCGAGGGGCCGGTGGTGGCGGTggttc                                                                                                    >  2:243433/1‑131 (MQ=255)
                                    ttCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCgg                                                                                                 >  8:302476/1‑136 (MQ=255)
                                    ttCTACGAAGAGCAGGGTGTGCCAGTCCGTTTTGTCGGCCACCCGCTGCCAGACACCATACCGCTTGAAGCCTACCGCCCGGCCCCCGCCTCCCCCCCGCGCCCCGGGCGCGGGCCCGTTGGGGCCGTTGATGccc                                                                                                  >  8:296220/1‑135 (MQ=255)
                                      cTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CCGCGCGTGCCGCGCGGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGc                                                                                               <  3:146355/136‑1 (MQ=255)
                                            agagCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCgg                                                                                         >  7:172559/1‑136 (MQ=255)
                                                 aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CTGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTg                                                                                     >  2:64101/1‑135 (MQ=255)
                                                 aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGCTGCCGGGCAGGCGGGGTgg                                                                                    >  8:154036/1‑136 (MQ=255)
                                                 aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTgg                                                                                    >  7:151811/1‑136 (MQ=255)
                                                 aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CCGCGCGTGCCGCGCGGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTg                                                                                     >  2:237454/1‑135 (MQ=255)
                                                 aGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CCGCGCGTGCCCCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCCTTGATGCCGGGCCGCCGGGGTgg                                                                                    >  3:236957/1‑136 (MQ=255)
                                                    gtgtGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGGTGATGCCGGGCAGCCGGGGGGGCg                                                                                  >  7:13735/1‑135 (MQ=255)
                                                           aGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CCGCGCGTGGCCCGCTGGGCCGGGGCGCGGGGCCGGTGGGGGCGGTGCTGCCGGGCAGCCGGGGTGGGGAAGTAggg                                                                          >  6:41170/1‑136 (MQ=255)
                                                           aGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAgg                                                                           >  1:3985/1‑135 (MQ=255)
                                                           aGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAgg                                                                           >  1:272460/1‑135 (MQ=255)
                                                           aGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CCGCGCGTGCCGCGCAGGGGCTGGGCGAGGGGGCGGTGGTGGGGTTGCTGCCGGGCAGCCGGGGTGGGGAAGTAgg                                                                           >  2:301574/1‑135 (MQ=255)
                                                           aGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CCGCGCGGGCCGCGGGGGGGCGGGGCGGGGGGGCGGGGGGGGCGGTGTGGGCGGGGCGCCCGGGGGGGGGAGTTggg                                                                          >  5:184214/1‑136 (MQ=255)
                                                           aGTGCGTTATGTCGGCCACCCGCTGGCAGACACCATACCGCAAGAAGCCGACCGCCCCG‑CCGCGCCTGCCGCGCTGGGCCTGGGCGAGCGGCCGGAGGAGGCGTTGATGCACGGCAGGCGGGGTGGCGAAGTAggg                                                                          >  6:152829/1‑136 (MQ=255)
                                                                gTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CCGCGCGTGGCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGCTGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCt                                                                      >  2:224704/1‑135 (MQ=255)
                                                                gTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGTTGCCGGGCCGCCGGGGGGGCGGAGTAGGGCGCtt                                                                     >  6:278934/1‑136 (MQ=255)
                                                                   ttGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CCGCGCGGGCCGCGCTGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTgg                                                                   <  6:94154/135‑1 (MQ=255)
                                                                    tGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGgc                                                                 >  5:171442/1‑136 (MQ=255)
                                                                     gtggcccACCCGCGGCCACAAACCAACCCGCTTCAACCCGCCCGCCGGC‑CCGGGCGGCCCGCGCGGGGCCGGGGCGCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGgc                                                                 <  7:167264/130‑1 (MQ=255)
                                                                             cccGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CCGCGCGTGCCGCGCTGGGCCGGGGCGCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGtt                                                         <  2:3985/135‑1 (MQ=255)
                                                                             cccGCTGGAACACACCATACCGCTTGACGCCGCCCGCCCGG‑CCGCGCGGGCCGCGCGGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGtt                                                         <  2:272460/135‑1 (MQ=255)
                                                                                gCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGC‑CCGCGGGTGCCGCGCGGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCt                                                      <  2:9646/135‑1 (MQ=255)
                                                                                               aCCGCTTGAAGCCGACCGCCCGG‑CCGCGCGTGCCGCGCTGGGCCAGGGCGCGGGGCCGGTGGTGGCGTTGATGCCGGGCCGCCGGGGGGGCGAAGTAGGGCGCTTGGGCGCGCTGGTCCTCGCCGCCGCCGAgcgc                                      >  7:129337/1‑136 (MQ=255)
                                                                                                  gCTTGAAGCCGACCGCCCGG‑CCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCt                                    >  4:275271/1‑135 (MQ=255)
                                                                                                      gAAGCCGACCGCCCGG‑CCGCGCGTGGCGCGCGGGGCCAGGGCGCGGGGCCGGTGGTGGCGTTGATGCCGGGCCGGCGGGGTGGCGGAGTAGGGCGCTTGGGCGCGCGGGTACGCGCCGCCGCCGCGCGccgccgc                                >  1:310990/1‑130 (MQ=255)
                                                                                                      gAAGCCGACCGCCCGG‑CCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGGGTTGATGCCGGGCCGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGCGCGCCTCAGc                                >  2:110768/1‑135 (MQ=255)
                                                                                                       acgccgccCGCCGGG‑CGGGGCGGGCCGCGCGGGGCCGGGGGGCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCca                              <  5:85161/134‑1 (MQ=255)
                                                                                                       aaGCCGACCGCCCGG‑CCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGcc                               >  8:234453/1‑135 (MQ=255)
                                                                                                         gcggcccGCCCGG‑CGGCGGGGGCGGCGCTGGGCGGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCcaac                            <  6:270697/131‑1 (MQ=255)
                                                                                                            gcccGCCCGG‑CCGCGCGGGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAg                         <  5:62018/134‑1 (MQ=255)
                                                                                                            ccccgccgGG‑CGGCGGGGGCCGGGGGGGGCGGGGGGGCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAg                         <  5:238241/134‑1 (MQ=255)
                                                                                                                   ggg‑CCGGGCGGGCCGCGCGGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCggg                  <  7:141221/135‑1 (MQ=255)
                                                                                                                        tgcgcgTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGgtgt               <  1:64101/134‑1 (MQ=255)
                                                                                                                        cgggggTGCCGGGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGgtgt               <  1:243433/130‑1 (MQ=255)
                                                                                                                         gcgcgGGCGGCGCTGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTgc             <  7:46233/136‑1 (MQ=255)
                                                                                                                         gcgcgGGCCGCGCTGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTgc             <  7:46235/136‑1 (MQ=255)
                                                                                                                         gcgcgGGCCGCGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTgc             <  3:83177/136‑1 (MQ=255)
                                                                                                                          cgggggCCGCGGGGGGCGTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTgc             <  1:114557/130‑1 (MQ=255)
                                                                                                                          cgcgTGCCGCGCTGGGCCTGGGGGCGGGGGCGGTGGTGGGGTTGTTTCCGGGCCGCCGGGGGGGCGCAGTAGGGCGCTTGGGCGCGCGGTTGCCCGCCGCCGCCGCGCGCCGCCGCCACCCAGTGGCggggtgggg            >  6:3252/1‑130 (MQ=255)
                                                                                                                                    gcTGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTCGTGTTg  <  6:171442/136‑1 (MQ=255)
                                                                                                                                      tgggcctgggcGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGaa                                                                                >  6:59302/1‑56 (MQ=255)
                                                                                                                                      tgggcctgggcGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGaa                                                                                <  5:59302/56‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                     
GACCTGATGCTGACGCTGCTGCCGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCCGCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGG‑CCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTCGTGTTG  >  ACB122/1791545‑1791811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑