Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	4,385,002	0	G	C	50.0%	1.5 / 10.0	18	intergenic (‑374/+96)	CJ019_00698/CJ019_04138	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (4/5);  new base C (0/9);  total (4/14)
Fisher's exact test for biased strand distribution p-value = 8.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CTTGCCTCCGGCTAACACTTCCCCTTGCCGGGTGTGTAGAGGACTTTCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGCAA‑CGCGCCATGCCTGGCGCACACAAAAAGGCCCGCACTTGGCGGGCCTTGGTGGATGATTCAGTTACGATGCCGCGTACTGCAGGCGG  >  ACB122/4384934‑4385122                                                                     |                                                                                                                          cTTGCCTCCGGCTAACACTTCCCCTTGCCGGGTGTGTAGAGGACTTTCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGAAA‑CGCGCCATGCCTGGCGCACACAAAAAGGCCCg                                                        <  6:128063/135‑1 (MQ=17)     ccTCGGGCTAACAATTCCCATTGCGGGGTGTGTACCGTACTTCCACCACCAAGTCACCCACTGGCCCACAACAACCCGCCGGGTTG‑GCGTAAGCGGAACCGCGCCATGCCTGGCGCACACAAAAAGGCCCGCACtt                                                   <  8:326654/136‑1 (MQ=2)       tCCGGCAACCCCTTCCCCTTCCCGGGTGTGAAGTGGACTTCCACCTCCATGCCCCCCAGTTGCCCCCCACACCCACGGGGGTTGCGCTTACGCGCAC‑CGCGCCATGCCTGGCGCACACAAAAAGGCCCGCACTTg                                                  <  6:337757/135‑1 (MQ=11)           gCTAACACTGCCCCTGGCGGGGGGTTGTGCGGATTTTCCCCTCAACGCCCCCCACTGGCCCACCACAACAACGCGGGTGGCGATTCCGCCAAA‑CGCGCCATGCCTGGCGCACACAAAAAGGCCCGCACTTGGc                                                <  4:247203/133‑1 (MQ=2)             tAACACTTCCCCTTGCCGGGTGTGTAGAGGACTTTCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTTCGCGTACGCGCCA‑CCCGCCAGGCCTGGCGCCCCACAAAAGGCCCGCCCGTGGCgggg                                            >  5:405000/1‑134 (MQ=11)                   ttCCCCTTGCCGGGTGTGTAGAGCACTTCCACCTCCAAGTCACCCACTTGCCCACCACAACCAAGCGGGTGGCGCTAACGCGAAA‑CGCGCCATGCCTGGCGCACACAAAAAGGCCCGCACTTGGCGGGCCTtggt                                      <  6:400702/135‑1 (MQ=14)                        cTTGCCGGGTGTGTAGAGGACTTTCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGCAA‑CGCGCCATGCCGGGCGCACACAAAAACGCCCGCCCTTGGCGGGCCGTGGTGgatga                                >  8:349531/1‑136 (MQ=255)                        cTTGCCGGGTGTGTAGAGGACTTTCACCTCCAAGGCACCCCGTTGGCCACCACGACCAAGGGGGTTGCGCTTACGCGCAA‑CGCGCCATGCCTGGCGCACACAAAAAGGCCCGCACTTGGCGGGCCTTGGTGgatga                                <  8:249454/136‑1 (MQ=17)                             cGGGTGTGTAGAGGACTTTCACCTCCACGGCACCCACTTGGCCACAACAACCAAGCGGGTTGCGCTTACGCGAAA‑CGCGCCATGCCTGGCGCACACAAAAAGGCCCGCACTTGGCGGGCCTTGGTGGATGATTCAg                           <  8:251292/136‑1 (MQ=255)                             cGGGTGTGTAGAGGACTTTCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGCAA‑CGCGCCATGCCTGGCGCACACAAAAAGGCCCGCACGTGGCGGGCCGTGGTGGATGATTCa                            >  4:252274/1‑135 (MQ=255)                                gtgggtAGAGGAGTTCCACCGCCACCGACCCCAGTGGCCCACCACAACCACGGGGTTCGCGCTAACGCCAAA‑CGCGCCATGCCTGGCGCACACAAAAAGGCCCGCACTTGGCGGGCCTTGGTGGATGATTCAGtt                         <  4:10099/135‑1 (MQ=12)                                   tgtAGAGGCCTTACACCTCCACGTCCCCCACTTGCCCACCACAACCAAGCGGGTGGCGCTAACGCGCAA‑CGCGCCATGCCTGGCGCACACAAAAAGGCCCGCACTTGGCGGGCCTTGGTGGATGATTCAGTTACg                      <  4:438273/135‑1 (MQ=18)                                                cACCTCCACGCCCCCCACTGGGCCCCCACAACCACGCGGGTTGCGCTTACGCCCAA‑CGCGCCATGCCTGGCGCACACAAAAAGGCCCGCACTTGGCGGGCCTTGGTGGATGATTCAGTTACGATGCCGCGTACTGc        <  7:224698/136‑1 (MQ=21)                                                 aCCTCCAAGCCACCCATTTGCCCCCCACACCCACGCGGGTTGCGATACCGCCCAA‑CGCGCCATGCCTGGCGCACACAAAAAGGCCCGCACTTGGCGGGCCTTGGTGGATGATTCAGTTACGATGCCGCGTACTGc        <  4:393440/135‑1 (MQ=21)                                                    cccAAGACCCCCACTGGCCCACCACAACCACGCGGGTGGCGCTAACCCGCAA‑CGCGCCATGCCTGGCGCACACAAAACGGCCCGCACTTGGCGGGCCTTGGTGGATGATTCAGTTACGATGCCGCGTACTGCAGGc    <  7:85132/135‑1 (MQ=21)                                                     ccAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTTCGCGCCA‑CGCCCCCTGCCGGGCGCACACAAAAACGCCCGCCCGTGGCGGGCCGTGGGGGTTGATTAAGGTTCGATGCCGCGGACTGCAGGc    >  2:49074/1‑135 (MQ=17)                                                       acggcACCCCCTTGGCCACCCCACCCACGCGGGGTGCGCTTACGCGCAA‑CGCGCCATGCCTGGCGCACACAAAACGGCCCGCACTTGGCGGGCCTTGGTGGATGATTCAGTTACGATGCCGCGTACTGCAGGCgg  <  6:83775/131‑1 (MQ=25)                                                       aaGTCACCCACTTGCCCACCACAACCACGCGGGTTGCGCTAACGCCCAA‑CGCGCCATGCCTGGCGCACACAAAAAGGCCCGCACTTGGCGGGCCTTGGTGGATGATTCAGTTACGATGCCGCGTACTGCAGGCgg  <  1:94376/135‑1 (MQ=255)                                                                     |                                                                                                                          CTTGCCTCCGGCTAACACTTCCCCTTGCCGGGTGTGTAGAGGACTTTCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGCAA‑CGCGCCATGCCTGGCGCACACAAAAAGGCCCGCACTTGGCGGGCCTTGGTGGATGATTCAGTTACGATGCCGCGTACTGCAGGCGG  >  ACB122/4384934‑4385122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑