| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,316,655 | 0 | T | G | 52.2% | 9.0 / 19.8 | 23 | T99P (ACG→CCG) | CJ019_03117 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (6/5); new base G (0/12); total (6/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.58e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTGCAAGTGCTCAGCCAGTTCCTCTGGCAACGGCACGAAGCGTACAAACGCGTGCAAGTGATGCGCCTCGCGGGTTACCTGCTTGATGCGCCGTTGCAGCTCACTGCCCAGCCGGTCACCGGCCAGCATTGCC‑GTGCGGTCGCCATGGGCGACGCGCCACAGCACTTCATACAGCAGGTTCCAGCGCTGCTCGCCACGGTAGCGAGAGGCCTGCTCCAACTGGCTCAGCAGTGCGGCCGGG > ACB122/3316520‑3316761 | gCTGCAAGTGCTCAGCCAGTTCCTCTGGCAACGGCACGAAGCGTACAAACGCGTGCAAGTGATGCGCCTCGCGGGTTACCTGCTTGATGCGCCGTTGCAGCTCACTGCCCAGCCGGTCACCGGCCAGCATTGCC‑GTg > 3:127099/1‑137 (MQ=255) cTGCAAGTGCTCAGCCAGTTCCTCTGGCAACGGCACGAAGCGTACAAACGCGTGCAAGTGATGCGCCTCGCGGGTTACCTGCTTGATGCGCCGTTGCAGCTCACTGCCCAGCCGGTCACCGGCCAGCATTGCC‑GTGc < 6:41212/137‑1 (MQ=255) tCCTCTGGCAACGGCACGAAGCGTACAAACGCGTGCAAGTGATGCGCCTCGCGGGTTACCTGCTTGATGCGCCGTTGCAGCTCACTGCCCAGCCGGTCACCGGCCAGCATTGCC‑GGGCAGTCGCCATGGGCGAcgcg < 6:223292/137‑1 (MQ=255) ctctGGCAACGGCACGAAGCGTACAAACGCGTGCAAGTGATGCGCCTCGCGGGTTACCTGCTTGATGCGCCGTTGCAGCTCACTGCCCAGCCGGTCACCGGCCAGCATTGCC‑GTGCGGGGGCCCTGGGCGCCCCGcc > 8:246719/1‑137 (MQ=255) ctctGGCAACGGCACGAAGCGTACAAACGCGTGCAAGTGATGCGCCTCGCGGGTTACCTGCTTGATGCGCCGTTGCAGCTCACTGCCCAGCCGGTCACCGGCCAGCATTGCC‑GGGCGGGCGCCATGGGCGACGCGcc < 6:284133/137‑1 (MQ=255) aCGGCACTAAGGGTACAACGGCGTGAATGTGATGCGCCTCGGGGGTTACCTGCTTGATGCGCCTTTGCAGGGCACTCCCCCGCGGGTCACCGGCCTGCATTGCC‑GGGGGGGCGCCTTGGGCGACGCGCCACAGCACt < 6:310411/137‑1 (MQ=255) gCAAGTGATGCGCCTCGCGGGTTACCTGCTTGATGCGCCGTTGCAGCTCACTGCCCAGCCGGTCACCGGCCAGCATTGCC‑GTGGGGTAGCCCGCGGGGCCCCCCCCCCCCACTTCATAAACCACGTTACAgcgcata > 7:83725/1‑134 (MQ=255) aGTGATGCGCCCCGCGGGTTACCTGCTTGATGCGCCGTTGCAGCTCACTGCCCAGCCGGTCACCGGCCAGCATTGCC‑GTGCGGGCGCCATGGGCGACGCGCCACAGCACTTCATACAGCAGGTTCCAGCGCTGCTCg < 5:218989/137‑1 (MQ=255) aGTGAGGCGCCTCGCGGGTTACCTGCTTGATGCGCCGTGGCAGCTCACTCCCCCGCCGGGCCCCGGCCAGCATTGCC‑GGGCGGGCGCCATGGGCGACGCGCCACAGCACTTCATACAGCAGGTTCCAGCGCTGCTCg < 2:53215/137‑1 (MQ=255) tGATGCGCCTCGGGGGTTACCTGCTTGATGCGCCGTTGAAGCTCACTGCCCCGCCGGGCACCGGCCAGCTTTGCC‑GGGGGGTCGCCATGGGCTACGCGCCACAGCACTTCATACAGCAGGTTCCAGCGCTGCTCGcc < 5:122943/137‑1 (MQ=255) aTGCGCCTCGCGGGTTACCTGCTTGATGCGCCGTTGCAGCTCACTGCCCAGCCGGTCACCGGCCAGCATTGCC‑GTGCGGGCGCCATGGGCGACGCCCCCCACCCCTTCAGACAGCACGTTCCAGCGCTGCTCGCCAc > 8:271353/1‑137 (MQ=255) ccgcgggggTTACCTGCTTGATGCGCCGTTGCAGCTCACTGCCCAGCCGGTCCCCGGCCAGCATTGCC‑GGGGGGGCGCCATGGGCGACGCGCCACAGCACTTCATACAGCAGGTTCCAGCGCTGCTCGCCACGGTAg < 8:193170/131‑1 (MQ=255) cgcgGGTTACCTGCTTGATGCGCCGTTGCAGCTCACTGCCCAGCCGGTCACCGGCCAGCATTGCC‑GTGGGGGGGCCCGGGGGGGCGCGCCCCAGCCCGGGATACAGCCGGTTGCAGCGCGGCTCGCCACGGTGGCgg > 7:299535/1‑136 (MQ=255) gCTTGTGGCGCGGTTGCAGCTCAGGCCCCCGCGGGTCCCCGCCCAGC‑TTGCCGGGGGGGGGGCCATGGGCGACGCGCCACAGCACTTCATACAGCAGGTTCCAGCGCTGCTCGCCACGGTAGCGAGAGGCCTGCTcc < 3:54407/137‑1 (MQ=255) gCTTGAGGCGCCGTGGCAGCTCACTCCCCAGCCGGTCACGGGCCAGCATTGCC‑GTGCGGTCGCCATGGGCGACGCGCCACAGCACTTCATACAGCAGGTTCCAGCGCTGCTCGCCACGGTAGCGAGAGGCCTGCTcc < 7:271353/137‑1 (MQ=255) gTTGCAGCTCACTGCCCCGCCGGTCACCGCCCAGCATGGCC‑GGGCGGTCGCCATGGGCGACGCGCCACAGCACTTCATACAGCAGGTTCCAGCGCTGCTCGCCACGGTAGCGAGAGGCCTGCTCCAACTGGCTcagc < 7:246719/137‑1 (MQ=255) ttGCAGCTCACTGCCCAGCCGGTCACCGGCCAGCATTGCC‑GTGCGGTCGCCATGGGCGACGCGCCACAGCACTTCATACAGCAGGTTCCAGCGCTGCTCGCCACGGTAGCGAGAGGCCTGCTCCAACTGGCTcagca < 4:222951/137‑1 (MQ=255) ttGCAGCTCACTGCCCAGCCGGCCACCGGCCAGCATTGCC‑GGGCGGTCGCCATGGGCGACGCGCCACAGCACTTCATACAGCAGGTTCCAGCGCTGCTCGCCACGGTAGCGAGAGGCCTGCTCCAACTGGCTcagca < 1:20198/137‑1 (MQ=255) cccACTCCCCCGCCGTGCCCCGCCAAGCATGGCC‑GTGCGGTCCCCAGGGCCTACGCGCCACAGCACTTCATACAGCAGGTTCCAGCGCTGCTCGCCACGGTAGCGAGAGGCCTGCTCCAACTGGCTCAGCAGTGCgg < 2:56468/135‑1 (MQ=255) cTCACCGCCCCCCCGTCCACCGGCCAGCATTGCC‑GGGCGGGCCCCAGGGGCGACGCGCCACAGCACTTCATACAGCAGGTTCCAGCGCTGCTCGCCACGGTAGCGAGAGGCCTGCTCCAACTGGCTCAGCAGTGCgg < 2:60995/137‑1 (MQ=255) aCTGCCCAGCCGGTCACCGGCCAGCATTGCC‑GTGGGGGGGCCCTGGGGGCCGCGCCCCCCCCCATCATACACCACGTTGCCGCGCGGCGCGCCCCCGGGGCGCGAGGGCGGCGCCCCCTGGCGCAGCCGTGCgggcc > 2:56597/1‑134 (MQ=255) tGCCCCGCCGGTCACCGGCCTGCATGGCC‑GGGGGGGCGCCTGGGGCGACGCGCCACAGCACTTCATACAGCAGGTTCCAGCGCTGCTCGCCACGGTAGCGAGAGGCCTGCTCCAACTGGCTCAGCAGTGCGGCCggg < 4:22176/137‑1 (MQ=255) ggCCCCGCCGGTCACCGGCCAGCATTGCC‑GGGCGGTCGCCATGGGCGACGCGCCACAGCACTTCATACAGCAGGTTCCAGCGCTGCTCGCCACGGTAGCGAGAGGCCTGCTCCAACTGGCTCAGCAGTGCGGCCggg < 8:82288/136‑1 (MQ=255) | GCTGCAAGTGCTCAGCCAGTTCCTCTGGCAACGGCACGAAGCGTACAAACGCGTGCAAGTGATGCGCCTCGCGGGTTACCTGCTTGATGCGCCGTTGCAGCTCACTGCCCAGCCGGTCACCGGCCAGCATTGCC‑GTGCGGTCGCCATGGGCGACGCGCCACAGCACTTCATACAGCAGGTTCCAGCGCTGCTCGCCACGGTAGCGAGAGGCCTGCTCCAACTGGCTCAGCAGTGCGGCCGGG > ACB122/3316520‑3316761 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |