| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 3,290,008 | T→G | 21.0% | intergenic (‑110/‑42) | CJ019_03093 ← / → CJ019_03094 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,290,008 | 0 | T | G | 21.0% | 61.0 / 2.7 | 50 | intergenic (‑110/‑42) | CJ019_03093/CJ019_03094 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (13/24); new base G (8/3); total (23/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.98e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATGCGAACAGCAGGGGTTTGTACATGGTGCCTCCGGCGTGGGTGCTAGGAAGCCCCATGCTTTCAGGCTGACGTCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCGTTGCG‑AGCCATTTGCCCCTGATGTTGCAAGGAACATTCGATG‑ACACACGTGCAGCGCCTCAAATATTCACTTCTGATCATCCTGGTGGTACTGGGCCTGATGCTGGGCCTGTCCCACCTGCAGAAGCAGGG > ACB122/3289873‑3290141 | gATGCGAACAGCAGGGGTTTGTACATGGTGCCTCCGGCGTGGGTGCTAGGAAGCCCCATGCTTTCAGGCTGACGTCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCt > 4:297718/1‑136 (MQ=255) tGCGAACAGCAGGGGTTTGTACATGGTGCCTCCGGCGTGGGTGCTAGGAAGCCCCATGCTTTCAGGCTGACGTCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCg > 8:381319/1‑136 (MQ=255) tGCGAACAGCAGGGGTTTGTACATGGTGCCTCCGGCGTGGGTGCTAGGAAGCCCCATGCTTTCAGGCTGACGTCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCg > 4:346881/1‑136 (MQ=255) gAACAGCAGGGGTTTGTACATGGTGCCTCCGGCGTGGGTGCTAGGAAGCCCCATGCTTTCAGGCTGACGTCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCGtt > 1:301848/1‑135 (MQ=255) agGGGTTTGTACATGGTGCCTCCGGCGTGGGTGCTAGGAAGCCCCATGCTTTCAGGCTGACGTCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCGCGGTGCG‑CGCCa > 8:209693/1‑136 (MQ=255) agGGGTTTGTACATGGTGCCTCCGGCGGGGGGGCTAGGAAGCCCCATGCTTTCAGGCTGACGTCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCGTTGCG‑AGcc < 6:180989/135‑1 (MQ=255) tGTACATGGTGCCTCCGGCGTGGGTGCTAGGAAGCCCCATGCTTTCAGGCTGACGTCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCGTTGCG‑CGCCATTTGccc > 8:24333/1‑136 (MQ=255) tACATGGTGCCTCCGGCGTGGGTGCTAGGAAGCCCCATGCTTTCAGGCTGACGTCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCGTTGCG‑AGCCATTTGccc < 6:334968/134‑1 (MQ=255) aCATGGTGCCTCCGGCGTGGGTGCTAGGAAGCCCCATGCTTTCAGGCTGACGTCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACAGTTGTATCATTGCCGCGGCGACGAGCG‑TGCCATTTGgcccg > 2:162977/1‑134 (MQ=255) cagggggCCTCCGGCGTGGGGGCTAGCAAGCCCCATGCTTTCAGGCTGACGTCACCCCACCCTGCAGGAATTCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCGTTGCG‑AGCCATTTGCCCCTg < 5:140172/129‑1 (MQ=255) cATGGTGCCGCGGGGGGGGGGGCTCGCACGCCCCAGGATTGCAGGCGCCCGCCACCCCACCCCCCTGCAAACCCCGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCGTTGCG‑AGCCATTTGCCCCTg < 2:158503/135‑1 (MQ=255) tGGTGCCTCCGGCGTGGGTGCTAGGAAGCCCCAGGCTTTCAGGCTGACGTCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCGTTGCG‑AGCCATTTGCCCCTGATg < 3:175069/136‑1 (MQ=255) gCCTCCGGCGTGGGTGCTAGGAAGCCCCATGCTTTCAGGCTGACGTCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGGGCGGTTCGCCGCCGTTTGCCCCGGGTGTTg > 8:148491/1‑136 (MQ=255) cTCCGGCGTGGGTGCTAGGAAGCCCCATGCTTTCAGGCTGACGTCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCGTTGCG‑AGCCATTTGCCCCTGATGTTGCa < 1:305835/135‑1 (MQ=255) ccGGCGTGGGTGCTAGGAAGCCCCATGCTTTCAGGCTGACGTCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCACGTTGCG‑AGCCCATTTCCCCTGCTCTTGCAAgg > 7:384460/1‑136 (MQ=255) tAGGAAGCCCCATGCTTTCAGGCTGACGTCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCGTTGCG‑AGCCATTTGCCCCTGATGTTGCAAGGAACATTCGATg < 5:120502/134‑1 (MQ=255) ccATGCTTTCAGGCTGACGTCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCGAGGTGCG‑CCCCAATTTCCCCTGAATTTGCAAGGAACATTGGATG‑ACACACgggg > 1:205556/1‑132 (MQ=255) cATGCTTTCAGGCTGACGTCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCGTTGGG‑GGCCCTTTGCCCCATATGTTGGAAGGAACATTGGTAG‑ACCCCCGTGCac > 3:371339/1‑135 (MQ=255) cATGCTTTCAGGCTGACGTCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCGTTGCG‑AGCCATTTGCCCCTGATGTTGCAAGGAACATTc < 5:128790/120‑1 (MQ=255) cATGCTTTCAGGCTGACGTCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCGTTGCG‑AGCCATTTGCCCCTGATGTTGCAAGGAACATTc > 6:128790/1‑120 (MQ=255) cATGCTTTCAGGCTGACGTCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCGCGGTGCG‑GGCCCTTTGGCCCGGGTGTTGCAAGGGACATTCGATG‑ACACACCTGcc > 5:353033/1‑134 (MQ=255) aTGCTTTCAGGCTGACGTACCCCCACCCAGAAGCAAATCCAGCGCCGGGGGCGGGGACCGTTGTATCATTGCCGCGGCTCGTTGCG‑AGCCATTTGCCCCTGATGTTGCAAGGAACATTCGATG‑ACACACGTGCAg < 6:319216/135‑1 (MQ=255) cAGGCGCACGTCCCCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCGTTGCG‑AGCCATTTGCCCCTGATGTTGCAAGGAACATTCGATG‑ACACACGTGCAGCGCCt < 6:126440/133‑1 (MQ=255) ggCTGACGTCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCGTTGCG‑AGCCATTTGCCCCTGCTGTTGCAAGGAACATTCGATGTACCCA‑GTGCAGCGCCGCaaa > 7:325226/1‑135 (MQ=255) gcgcccGTCACCCAACCCAGCCGTAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCGTTGCG‑AGCCATTTGCCCCTGATGTTGCAAGGAACATTCGATG‑ACACACGTGCAGCGCCTCAAat < 5:176553/130‑1 (MQ=255) gCTGACGTCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCTCGGGGCGTTGCG‑AGCCATTTGGCCCATATGTTGCAAGGAACATTCGATT‑CCACACGTGCAGCGCGGCAAata > 8:328405/1‑136 (MQ=255) gCTGACGTCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCGTTGCG‑AGCCATTTGCCCCTGATGTTGCAAGGAACATTCGATG‑ACACACGTGCAGCGCCTCAAat < 7:39887/135‑1 (MQ=255) gTCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCGTTGGG‑AGCCATTTGCCCCTGATGTTGCAAGGAACATTCGATG‑ACACACGGGCAGCGCCACAAATTTTCACt > 4:106844/1‑136 (MQ=255) tCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGGTCGGTGCG‑CGCCATTTGGCCCGGATGTTGCAATGGACATTCGATG‑CCACCCGGGCAGCGCCTCAAATATTCACt > 2:248675/1‑135 (MQ=255) tCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGGGCGGTGCG‑CGCCCTTTGCCCCGGATGTTGGAAGGAACAATCGATG‑ACCCACCTGCAGCGCCGCCAATAttaaa > 5:117737/1‑131 (MQ=255) tCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCGCCGTTCG‑CGCCCTTTTCCCCGGATTGTGCAACGAACAATCGATG‑TCACACCTTCCCCCCCGCAAATAttacaa > 7:40524/1‑131 (MQ=255) cccccaGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCCCGTTGCG‑AGCCATATGCCCAGACTGTTGCAAGCACCATAACATG‑ACACACCTCCAGGGCCTCAAATATTCACTTcagct > 8:88427/3‑131 (MQ=255) ctgcagGAAAGACAGCGCCGGGGGCGGTGTCCGTTGTATCATTGCCGCGGCTCGTTGCG‑AGCCATTTGCCCCTGATGTTGCAAGGAACATTCGATG‑ACACACGTGCAGCGCCTCAAATATTCACTTCTGATCATcc < 8:384460/134‑1 (MQ=255) cagGAAACCCCGGGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCGTTGCG‑AGCCATTTGCCCCTGATGTTGCAAGGAACATTCGATG‑ACACACGTGCAGCGCCTCAAATATTCACTTCTGATCATCCtg < 6:343602/135‑1 (MQ=255) gAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCGTTGCG‑CGCCATTTTCCCCAGATGTTTCAAGGAACATTCGATG‑ACACCCGTGCAGCGCCTCAAAAATTCACTTCTGATCATCCaggtg > 6:308933/1‑135 (MQ=255) gAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCGGTGCG‑AGCCATTTGCCCCTGATGTTGCAAGGAACATTCGATG‑ACACACGTGCAGCGCCTCAAATATTCACTTCTGATCATCCtggtgg < 7:52541/136‑1 (MQ=255) aaTCCCGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCGTGGCG‑AGCCATTTGCCCCTGATGTTGCAAGGAACATTCGATG‑ACACACGTGCAGCGCCTCAAATATTCACTTCTGATCATCCTggtggt < 7:209693/135‑1 (MQ=255) ccAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCGGTGCG‑AGCCCTTTTCCCCAGATGTTGCAAGGAACAATCGATT‑ACACACGTGCAGCGCCTCAAATATTCACTTATGATCATGCTTGTGGTAGTg > 8:347859/1‑136 (MQ=255) cAGCGCCGGTGGCGGTGTCCGTTCGATCATTGACGCGGCTCGGTGCG‑AGCCATTTGCCCCTGATGTTGCAAGGAACCTTCGATG‑ACACACGTGCAGCGCCTCAAATATTCACTTCTGATCATCCTGGTGGTACTgg < 8:276906/136‑1 (MQ=255) cgcgcCGGGGGCGGTGTCCGTTGTATCATTGCCGCGGCTCGTTGCG‑AGCCATTTGCCCCTGATGTTGCTAGGAACATTCGATG‑ACACACGTGCAGCGCCTCAAATATTCACTTCTGATCATCCTGGTGGTACTgg < 2:364990/134‑1 (MQ=255) gcgcCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCGCGTTGCG‑AGCCATTTGCCCCTGATGTTGCAAGGAACATTCGATG‑ACACACGTGCAGCGCCTCAAATATTCACTTCTGATCATCCTGGTGGTACTggg < 6:59923/135‑1 (MQ=255) cGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCGTTGCG‑AGCCATTTGCCCCTGATGTTGCAAGGAACATTCGATG‑ACACACGTGCAGCGCCTCAAATATTCACTTCTGATCATCCTGGTGGTACTGGGCCTGa < 7:333904/136‑1 (MQ=255) ggCGGTGACCGTTGTATCATTGCCGCGGCTCGTTGCG‑AGCCATTTGCCCCTGATGTTGCAAGGAACATTCGATG‑ACACACGTGCAGCGCCTCAAATATTCACTTCTGATCATCCTGGTGGTACTGGGCCTGATGCt < 8:331093/136‑1 (MQ=255) tGACCGTTGTATCATTGCCGCGGCGCGTTGCG‑AGCCATTTGCCCCTGATGTTGCAAGGAACATTCGATG‑ACACACGTGCAGCGCCTCAAATATTCACTTCTGATCATCCTGGTGGTACTGGGCCTGATGCTGGGcc < 7:28268/136‑1 (MQ=255) tGCCGCGCCTCGTTGCG‑AGCCATTTGCCCCTGATGTTGCAAGGAACATTCGATG‑CCCCACGTGCAGCGCCTCAAATATTCACTTCTGATCATCCTGGTGGTACTGGGCCTGATGCTGGGCCTGTCCCACCTGCa > 5:119686/1‑134 (MQ=255) gCCGCGGCTCGTTGCG‑AGCCATTTGCCCCTGATGTTGCAAGGAACATTCGATG‑ACACACGTGCAGCGCCTCAAATATTCACTTCTGATCATCCTGGTGGTACTGGGCCTGATGCTGGGCCTGTCCCACCTGCAGa < 8:252748/135‑1 (MQ=255) ccGCGGCTCGTTGCG‑AGCCCTTTGGCCCTGATGTTGCAAGGAACATTCGATG‑ACACACGTGCCGCGCCGCAAATATTCACTTCTGATCATCCTGGTGGTACTGGGCCTGATGCTGGGCCTGTCCCACCTGCAGAAg > 7:307288/1‑136 (MQ=255) ccGCGGCTCGTTGAG‑AGCCATTTGCCCCTGATGTTGCAAGGAACATTCGATG‑ACACACGTGCAGCGCCTCAAATATTCACTTCTGATCATCCTGGTGGTACTGGGCCTGATGCTGGGCCTGTCCCACCTGCAGa < 2:301848/134‑1 (MQ=255) cgcgGCTCGTTGCG‑AGCCATTTGCCCCTGATGTTGCAAGGAACATTCGATG‑ACACACGTGCAGCGCCTCAAATATTCACTTCTGATCATCCTGGTGGTACTGGGCCTGATGCTGGGCCTGTCCCACCTGCAGAAg < 6:353033/135‑1 (MQ=255) cgcgGCGCGTTGCG‑ATCCATATGCCCCTGATGTTGCAAGGAACATTCGATG‑ACACACGTGCAGCGCCTCAAATATTCACTTCTGATCATCCTGGTGGTACTGGGCCTGATGCTGGGCCTGTCCCACCTGCAGAAg < 1:162977/135‑1 (MQ=255) cTCGTTGCG‑AGCCATTTGCCCCTGATGTTGCAACGAAAATTCGATG‑AAACACGGGCAGCGCCTCAAATATTCACTTCTGATCATCCTGGGGGGACTGGGCCTGATGCTGGGCCTGTCCCACCTGCAGAAGCAggg < 5:251823/135‑1 (MQ=255) | GATGCGAACAGCAGGGGTTTGTACATGGTGCCTCCGGCGTGGGTGCTAGGAAGCCCCATGCTTTCAGGCTGACGTCACCCCACCCAGCAGGAAATCCAGCGCCGGGGGCGGTGACCGTTGTATCATTGCCGCGGCTCGTTGCG‑AGCCATTTGCCCCTGATGTTGCAAGGAACATTCGATG‑ACACACGTGCAGCGCCTCAAATATTCACTTCTGATCATCCTGGTGGTACTGGGCCTGATGCTGGGCCTGTCCCACCTGCAGAAGCAGGG > ACB122/3289873‑3290141 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |