| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 382,479 | T→G | 36.3% | L12R (CTG→CGG) | soxD → | Sarcosine oxidase subunit delta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 382,479 | 0 | T | G | 36.3% | 36.6 / 5.7 | 51 | L12R (CTG→CGG) | soxD | Sarcosine oxidase subunit delta |
| Reads supporting (aligned to +/- strand): ref base T (11/17); new base G (6/14); total (20/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATCCACTGGCCGCGCCGTTCTCCATGGACCGTTTCTACAACGGCGCACTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATA‑TTTTCTGTCCCCACTGCGGCGAGCTGCGCTCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTA‑CATGTTCTACCGTGACAACCCGCGCGGTATTCACCATGA > ACB122/382344‑382611 | cATCCACTGGCCGCGCCGTTCTCCATGGACCGTTTCTACAACGGCGCACTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATA‑TTTTCTGTCCCCACTGCGGCGAGCt < 8:51270/136‑1 (MQ=255)cATCCACTGGCCGCGCCGTTCTCCATGGACCGTTTCTACAACGGCGCACTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATA‑TTTTCTGTCCCCACTGCGGCGAGCt < 7:249083/136‑1 (MQ=255) gCCGCGCCTTTCTCCATGGACCGTTTCTACAACGGCGCACTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATA‑TTTTCTGTCCCCACTGCGGCGAGCTGCGCTCCg < 8:332178/135‑1 (MQ=255) ccGCGCCGTTCTCCATGGACCGTTGCTACAACGGCGCACTGATCGACTAACACGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATA‑TTTTCTGTCCCCACTGCGGCGAGCTGCGCTCCgaa < 7:3815/136‑1 (MQ=255) cgcCGTTCTCCATGGACCGTTTCTACAACGGCGCACTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCGGAGCCACCGTCATGTTGCATA‑TTTTCTGTCCCCACTGCGGCGGGCGGCGCGCCgca > 5:391427/1‑131 (MQ=255) gcCGTTCTCCATGGACCGTTTCTACAACGGCGCACTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATA‑TTTTCTGTCCCCACTGCGGCGAGCTGCGCTCCgaa > 2:110826/1‑132 (MQ=255) cGTTCTCCATGGACCGTTTCTACAACGGCGCACTGATCGACGAACCCGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGATGCATA‑TTTTCTGTCCCCACTGCGGCGCGCAGCGCGCCccagaaca > 6:17421/1‑133 (MQ=255) ccATGGACCGTTTCTACAACGGCGCACTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATA‑TTTTCTGTCCCCACTGCGGCGAGCTGCGCTCCGAAGAAGAGtt < 7:270275/132‑1 (MQ=255) ccATGGACCGTTTCTACAACGGCGCACTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATA‑TTTTCTGTCCCCACTGCGGCGAGCTGCGCGCCGAAGAAGAGtt > 8:270275/1‑132 (MQ=255) tttCTACACCGGCGAACTGACCGACGAACCCGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATA‑TTTTCTGGCCCCACTGCGGCGAGCTGCGCTCCGAAGAAGAGTTCCACGCCTCTGGcc < 7:46374/136‑1 (MQ=255) cTACAACGGCGCACTGATCGACGAACACGGCGCCGCCGCCTTCGCCCACTAACCGGAGACACCGTCATGTTGCATA‑TTTTCTGTACCACCTTCGGCGGGCAGCGCCCCGCAGAAAACTTCAACGCCTCTGGcccag > 8:235507/1‑133 (MQ=255) tACAACGGCGCACTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATA‑TTTTCTGTCCCCACTGCGGCGGGCTGCGCGCCGAAGAAGCGGTGCACGCCTCAGGGCCGgc > 3:295228/1‑136 (MQ=255) tACAACGGCGCACTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATA‑TTTTCTGTCCCCACTGCGGCGATCGGCGGTCCGCAGAAGAGTTACACGCCTCAGGCCCGgc > 3:268874/1‑136 (MQ=255) gcgcACTGATCGACGAACACGGCGCCGCCGCCGTCGCCCCCTAACCGGAGACACCGTCATGTTGCATA‑TTTTCTGTCCCCACTGCGGCGCGCTGCGCTCCCAATAAGAGTTGCACGCCTCGGGCCCggtctcgt > 6:70683/1‑128 (MQ=255) aCTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATA‑TTTTCTGTCCCCACTGCGGCGCGCGGGGCGCCGCAGAAGAGTTCCACCCCGCGGGCCCGGCGCGCATTgcca > 7:367795/1‑135 (MQ=255) aTCGACGAACACGGCGCCGCCGCCGGCGCCCACTAACCGGAGACACCGTCATGTTGAATA‑TTTTCTGGCCCCACTGCGGCGAGCGGCGCTCCGAAGAAGCGTGCCACGCCTCTGGCCAGGCGCACATTgcccgccc < 4:339941/136‑1 (MQ=255) tccccgaACCCGGCGCCGCCGCCTTCGCCCACTAACCGGAGACCCCGTCATGTTGAATA‑TTTTCTGTCCCCACTGCGGCGCGCTGCGCTCCGAAGAAGAGTGCCACGCCTCTGGCCAGGCGCACATTgcccgccc < 7:197541/131‑1 (MQ=255) gacgaACACGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATA‑TTTTCTGTCCCCACTGCGGCGAGCTGCGCTCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCccgcccgc > 2:78248/1‑135 (MQ=255) gacgaACACGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATA‑TTTTCTGTCCCCACTGCGGCGAGCGGGGCTCCGAAGCAGAGTTCCCCGCCGCTGGCCCGGCGCCCCTTGCccgcccgc > 6:189015/1‑135 (MQ=255) accccGGCGCCGCCGCCGTCGCCCACTAACCGCAGACACCGTCATGTTCCATA‑TTTTCTGCCCCCACTGCGGCGAGCGGCGCTCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGAc < 7:92167/132‑1 (MQ=255) aaCACGGCGCCGCCGCCGTCGCCCAGTAACCGGAGACACCGTCATGTTGCATT‑TTTTCTGTCCCCACTGCGGCGAGCTGCGCTCCTAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGAc < 3:186867/136‑1 (MQ=255) cacGGCGCCGCCGCCGTCGCCCAATAACCGGACACACCGTCTTGTTGCAAA‑TTTTCTGTCCCCACTGCGGCGAGCTGCGGTCCGAACACGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGAc < 6:164254/134‑1 (MQ=255) ggCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCTTGTTGCATA‑TTTTCTGTCCCCACTGCGGCGAGCTGCGCGCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTaa < 8:241409/136‑1 (MQ=255) gcgcCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATA‑TTTTCTGTCCCCACTGCGGCGAGCTGCGCTCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAAc > 8:308363/1‑136 (MQ=255) gccgccgTCGCCCACTAACCGGAGACACCGTCATGTTGCATA‑TTTTCTGTCCCCACTGCGGCGAGCGGCGCTCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCt < 1:78248/135‑1 (MQ=255) gccgccgTCGCCCACTAACCGGAGACACCGTAATGTTGCATA‑TTTTCTGTCCCCACTGCGGAGAGCTGCGCTCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCt < 6:154890/135‑1 (MQ=255) gccgccgTCGCCAAATAACGGGAGACCCCGTCATTTTGATTA‑TTTGCTTCCCCAACTGCGGCGCGCGGGGCTCGGATGATGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCt < 2:325715/135‑1 (MQ=255) ccgGCGCCCACTACCCGGAGACCCGGTCATGTTGCATA‑TTTTCTGCCCCCACTGCGGCGAGCGGCGCGCCGACGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTcc < 7:2855/136‑1 (MQ=255) gacGCCAACAAACCGGAGACCCCGGAATGTTCAATA‑TTTTCTGCCCCCACTGCGGCGAGCGGCGCTCCGAAGATTAGTGCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCcg < 2:102959/133‑1 (MQ=255) gTCCCCCACTAACCGGCGACACCGTCTTGTTG‑ATATTTTTCTGCCCCCCCTGCGGCTGGCGGCGCCCCGAAGAAGAGTGCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCcg < 6:220279/135‑1 (MQ=255) tCGCCCACTACCCGGACACACCGTCAGTGTGAATA‑TTTTCTGTCCCCCCTGCGGCGAGCGGCGCTCCGAAGCAGAGGTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCcgac < 7:135053/136‑1 (MQ=255) gCCCACTAACCGGAGACACCGTCATGTTGCATA‑TTTTCTGTCCCCACTGCGGCGAGCTGCGCTCCGAAGAAGAGtt > 8:257057/1‑76 (MQ=255) gCCCACTAACCGGAGACACCGTCATGTTGCATA‑TTTTCTGTCCCCACTGCGGCGAGCTGCGCTCCGAAGAAGAGtt < 7:257057/76‑1 (MQ=255) gaCACCGTCATGTTGCATA‑TTTTCTGCCCCCCCTGCGGCGAGCTGCGCGCCGAAGACGAGTCCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTAcc < 6:64630/135‑1 (MQ=255) cccacCGTCATGGTTCATA‑TTTTCTGTCCCCACGGCGGCGAGCGGCGCTCCGAAGAAGAGTCCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTAcc < 5:242980/133‑1 (MQ=255) cacGGTCATGTGGAATA‑TTTTCTGTCCCCTCTGCGGCGAGGTGCGCTCCGAAGAAGAGTACCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTA‑c < 4:174261/136‑1 (MQ=255) ccGTCATGTTGCATA‑TTTTCTGTCCCCACTGCGGCGAGCTGCGCTCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGCCCCAAACGCCTGCTCCGCCGAGGAGTGGGGTACCTA‑CAt > 8:208238/1‑136 (MQ=255) cATGTTGCATA‑TTTTCTGCCCCCACTGCGGCGCGCGGCGCTCCGAACAAGAGTGCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTA‑CATGtt < 2:220277/135‑1 (MQ=255) cATA‑TTTTCTGGCCCCCCTGCGGCGAGCTGCGCTCCGAAGAAGATTGCCACGCCTCTGGCCAGGCGCACTTTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGTGTACCTA‑CATGTTCTACCGt < 8:284009/135‑1 (MQ=255) ata‑tTTTCTGTCCCCACTGCGGCGCGCGGCGCGCCGAAGAAGAGTTCCACGCCTCTCGCCACGCGCACATTGCCCCCCCGCGGGCCCCGAACGCCGGCTCCGACGCGGAGAGGGGTACCGA‑CATGTTCTACCGTg > 5:311532/1‑135 (MQ=255) a‑tTTTCTGTCCCCACTGCGGCGCGCTGCCCGCCCAACAACACTTACACCCCGCAGGCCCCGCGCACAATGCCCGCCCCCAGGCCACAAACGCCAGCGCCCCCGAGGAGGGGGTGACCAACCATG‑TCTACCCTGACa > 4:86646/1‑136 (MQ=255) a‑tTTTCTGTCCCCACTGCGGCGAGCAGCGCGCCGAAGAAGAGTTCCACCCCTCTGGCCAGGCGCCCATTTCCCCCCCCCGGGCCCCGAACGCCGGCTCCCACGAGGCGTTGGGTACCTA‑CATTTTCTAACGTgg > 2:341299/1‑133 (MQ=255) cTGACCCCACTGCGGAGAGCTGGGCTCCGAAGAAGAGTCCCAGGGCTGTGGCCAGGCGCACATTGGCCGCCCGCTGGACCCTAACGCCTGCTCCGTCGAGGAGTGGGGTACCTA‑CATGTTCTACCGTGACAAccg < 3:155062/135‑2 (MQ=255) tgcccccccGGCGGCGTGCGGCCCTCGGAACACGCGTACCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTA‑CATGTTCTACCGTGACAAccc < 1:341299/131‑1 (MQ=255) tgcccccACTGCGGCGAGCGGCGCGCCGAATAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTA‑CATGTTCTACCGTGACAAccc < 1:110826/131‑1 (MQ=255) tGTCCCCACTGCGGCGAGCTGCGCTCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTA‑CATGTTCTACCGTGACAAccc < 1:40575/134‑1 (MQ=255) gTCCCCACTGCGGCGGGCTGCGCTCCGAAGAAGAGTTGCACGCCTCTGGCCAGGCGCCCATTGCCCGCCCGCTGGGCCCGAACGCCTGCTCCGGCGAGGGGTGGGGTAGCTA‑CATGTTGTACCGTGGCACCCcgc > 2:91052/1‑135 (MQ=255) ccgcgtGCGGCTCGCGGCGCTCCGACTTAGTGCGCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTA‑CATGTTCTACCGTGACATCCCGCGCgg < 7:337293/131‑1 (MQ=255) ccccCTGCGGCGAGCTGCGCTCCGAAGAAGCGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTA‑CATGTTCTACCGTGACAACCCGCGCgg < 8:67275/136‑1 (MQ=255) ccACTGCGGCGAGCGGCGCTCCGACGACGCGCCCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTA‑CATGTTCTACCGTGACAACCCGCGCgg < 5:259354/135‑1 (MQ=255) aCTGCGGCGCGCGGCGCTCCGTAGAAGACTTCCCCGCCTCTGGCCAGGCGCACATTGCCCGCCCGCGGGCCCCTAACCCCTGCTCCGACGAGGAGTGGGGTTCCTA‑CATGTTCTACCGTGACCACCCGCGCggga > 1:50847/1‑133 (MQ=255) ggcgAGCTGCGCTCCGAAGAAGAGTGCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTA‑CATGTTCTACCGTGACAACCCGCGCGGTATTCAcc < 7:208238/136‑1 (MQ=255) gCTGCGCTCCGAAGAAGAGTTACACGCCTCTGGCCCGGCGCACATTTCCCGCCCGCTGGGCCCTAACGCCTGCTCCGCCGAGGAGTGGGGTACCGA‑CATGTTCTACCGTGCCAACCCGCGCGGGATTCACCATGa > 8:254453/1‑135 (MQ=255) | CATCCACTGGCCGCGCCGTTCTCCATGGACCGTTTCTACAACGGCGCACTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCGGAGACACCGTCATGTTGCATA‑TTTTCTGTCCCCACTGCGGCGAGCTGCGCTCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTA‑CATGTTCTACCGTGACAACCCGCGCGGTATTCACCATGA > ACB122/382344‑382611 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |