Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	399,292	A→C	37.1%	intergenic (‑186/+122)	CJ019_00365 ← / ← aceF	hypothetical protein/Dihydrolipoyllysine‑residue acetyltransferasecomponent of pyruvate dehydrogenase complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	399,292	0	A	C	37.1%	31.6 / 4.4	49	intergenic (‑186/+122)	CJ019_00365/aceF	hypothetical protein/Dihydrolipoyllysine‑residue acetyltransferasecomponent of pyruvate dehydrogenase complex
Reads supporting (aligned to +/- strand):  ref base A (21/8);  new base C (10/8);  total (33/16)
Fisher's exact test for biased strand distribution p-value = 3.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGTCCCGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAAGGAGGGCGAAG‑CCC‑TCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCTTACAGCAGCATGG  >  ACB122/399169‑399427                                                                                                                            |                                                                                                                                          gatgTCCCGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAAGGAGGGCGAAG‑CCC‑t                                                                                                                             >  4:43355/1‑136 (MQ=255) gatgGCCCGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAAGGAGGGCGAAG‑ccc                                                                                                                               <  5:378559/135‑1 (MQ=255)       ccGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAAGGAGGGCGCAG‑CCC‑TCCCAGc                                                                                                                       >  7:14331/1‑136 (MQ=255)        cGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAAGGCGGGGGAAG‑CCC‑TCCCCGc                                                                                                                       >  5:41264/1‑135 (MQ=255)        cGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAAGGCGGGCGCAG‑CCC‑TCCCCGc                                                                                                                       >  6:375841/1‑135 (MQ=255)                 gCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGAGGCGCGGAAGGCGGGGGCAG‑CCC‑GCCCCGCgttttgcgg                                                                                                              >  2:330358/1‑131 (MQ=255)                  cAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAAGGAGGGCGAAG‑CCC‑TCCCAGCTTTTTCCAgg                                                                                                             >  7:310856/1‑135 (MQ=255)                    aGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAAGGAGGGCGCAG‑CCC‑TCCCAGCTTTTTCCCGGc                                                                                                            >  6:331112/1‑134 (MQ=255)                         ccATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAAGGAGGGCGAAG‑CCC‑TCCCCGCTTTTTCCAGGCaaaaaa                                                                                                      >  8:151318/1‑135 (MQ=255)                              cTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGTAAGGGGGGGGCAG‑CCC‑TCCCACCGTTTTTCAGGCAAAAAAAAcccc                                                                                                >  4:209466/1‑136 (MQ=255)                               ttGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAAGGAGGGCGCAG‑CCC‑TCCCAGCTTTTTCCAGGCAAAAAAAAGccc                                                                                                >  2:314825/1‑135 (MQ=255)                                     cTGTGGACAGGTTTTCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAAGGAGGGCGAAG‑CCC‑TCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAAt                                                                                         <  8:396593/136‑1 (MQ=255)                                      tgtgGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAAGGAGGGCGCAG‑CCC‑TCCCAGCGTTTTCCAGGCAAAAAAAAGCCCCGCCaaac                                                                                        >  7:23791/1‑134 (MQ=255)                                       gtgGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAAGGAGGGCGAAG‑CCC‑TCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCg                                                                                       <  8:310856/136‑1 (MQ=255)                                           accggTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTGGGGGCCGCTTTGCGGCCTTGTGTCCCGAAAGGAGGGCGAAG‑CCC‑TCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACgg                                                                                   <  8:395097/133‑1 (MQ=255)                                           aCAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGGGGCCTTGTGTCGCGGAACGGGGGGGGAG‑CCC‑GCCCCCCGTTTTGCATGCAAAAAAAAACCCCGCCCAacgacg                                                                                    >  5:340990/1‑135 (MQ=255)                                           aCAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTGGCGGAACGCGGGGGGAG‑CCCGGCCCCCGTTTTTCCGGGCAAAAAAAGCCCCCCCCaaaggccg                                                                                    >  3:13357/1‑130 (MQ=255)                                           aCAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCGTTGCGGCCGTGTGGCGCGGAAAGAGGGCGCCGCCCC‑ACCCCCCTTTTTCCGGCCAAAAAAACCCCCCCCCaaccccg                                                                                     >  6:38100/1‑130 (MQ=255)                                            cAGGTTCTCGGCGGACGGGGCCGATTCTGCAGTTTGGCGGGAGATTTTGGGGCCGCTTTGCGGCCTGGTGTCGCGAAAGGCGGGCGAAG‑CCC‑GCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACgg                                                                                   <  6:188821/135‑1 (MQ=255)                                                ttATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAAGGCGGGGGAAG‑CCC‑TCCCAGCATTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACCGGGGTg                                                                              >  4:128479/1‑136 (MQ=255)                                                ttATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAAGGAGGGCGAAG‑CCC‑TCCCAGCTTTTTCCAGGCAAAAAACAGCCCCGCCAATCGACGGGGTTg                                                                              >  7:370453/1‑136 (MQ=255)                                                ttATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAAGGAGGGCGAAG‑CCC‑GCCCAGCGTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGCCGGGGGTg                                                                              >  7:178178/1‑136 (MQ=255)                                                ttATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAACGAGGGCGGAG‑CCC‑GGCCCGCGTTTTCCAGGCTAAAAAAAGCCCCCCCAATCGCCGGGGtt                                                                               >  7:188687/1‑135 (MQ=255)                                                  aTCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGGAAGGAGGGCGCAG‑CCC‑GCCCCGCGTTTTGCAGGCAAAAAAAAGCCCCGCCCATCGACggggggggg                                                                            >  3:28742/1‑131 (MQ=255)                                                  aTCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGCAAGGCGGGCGACG‑CCC‑TGCCAGCGTTTTTCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAg                                                                            >  4:215530/1‑136 (MQ=255)                                                     ggCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAAGGCGGGCGAAG‑CCC‑TCCCAGCTTTTTCCCGGCAAAAAAAAGCCCCGCCCATCGCCGGGGTTGAGGTa                                                                         >  8:31233/1‑136 (MQ=255)                                                     ggCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAAGGAGGGGGAAG‑CCC‑GCCCAGGTTTTTGCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGGTGAGGt                                                                          >  6:283932/1‑135 (MQ=255)                                                       cTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAAGGAGGGCGAAG‑cc                                                                                                                                >  6:159279/1‑80 (MQ=255)                                                       cTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAAGGAGGGCGAAG‑cc                                                                                                                                <  5:159279/80‑1 (MQ=255)                                                            gTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGGCTTGTGTCGCGAAAGGCGGGCGAAG‑CCC‑TCCCAGGGTTTTCCAGGCAAAAAAAACCCCCGCCAAACGCCGGGGTTGAGGTACGAGCg                                                                   >  6:189897/1‑135 (MQ=255)                                                            gTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGCAAGGCGGGCGCAG‑CCC‑TCCCAGCGTTTTCCCGGCAAAAAAAAGCCCCGCCCATCGCCGGGGGTGAGGTAGGAGCg                                                                   >  1:100463/1‑135 (MQ=255)                                                            gTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAAGGAGGGCGAAG‑CCC‑TCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCg                                                                   >  2:12433/1‑135 (MQ=255)                                                            gTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAAGGAGGGCGAAG‑CCC‑TCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGt                                                                  >  7:50767/1‑136 (MQ=255)                                                             tGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAAGGAGGGCGAAG‑CCC‑TCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGCCGGGGTTGAGGTACGAGCGTg                                                                 >  7:327614/1‑136 (MQ=255)                                                                                 cTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGCAAGGCGGGCGAAG‑CCC‑TCCCCGCGTTTTGCAGGCCAAAAAAACCCCCCCCCATAGCCGGGGGTGAGGTTCCCGCGTGGGCGCTCGCAAAAGGTaag                                              >  6:8533/1‑133 (MQ=255)                                                                                 cTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAACGAGGGGGCAG‑CCC‑CCCCAGCGTTTTACAGGGCAAAAAAACCCCCCCCCAACGCCGCGGGTGAGGTACGCGCGTGGCCGCTCGcaaaacctaca                                              >  6:286773/1‑129 (MQ=255)                                                                                     agaTTTGGGGGCCGCTTTGGGGCCTGGTGTCGCGAACGGAGGGCGACG‑CCC‑CCCCAGCTTTTTCCAGGAAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGGGGCAGCTCGAAAAAGGTACTGcccc                                         <  8:394073/136‑1 (MQ=255)                                                                                         tttGGGGCCGCTTGGGGGCCTGGTGTCGCGAACGGCGGGCCACG‑CCC‑TCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTc                                       <  6:120234/134‑1 (MQ=255)                                                                                            ggggCCGGTTGGGGGCGTGGTGCCCCCAAGGGCGGGCCACG‑CCC‑TCCCAGCTTTTGCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAag                                  <  7:348788/136‑1 (MQ=255)                                                                                               gCCGCTTTGCGGCCTGGTGGCGCGAACGGCGGGCGAAG‑CCC‑TCCCAGCTTTTTCCAGGCAAAAAAACGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAaggag                               <  8:178178/136‑1 (MQ=255)                                                                                                ccGCTTTGCGGCCTTGTGCCGCGAAAGGCGGGCGAAC‑CCC‑TCCCAGCTTTTTCCAGGCAAAAAAACGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAaggagg                              <  4:28742/136‑1 (MQ=255)                                                                                                 cGCTTTGCGGCGGTGTGTCGCGAACGGCGGGCGAAG‑CCG‑TCCAAGCTTTTTCCAGGCAAAAAACAGCCCCGCCAATCGACGGGGTGGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAggg                             <  4:55841/136‑1 (MQ=255)                                                                                                       gCGGCCGGGTGCCCCGAACGGCGGCCCAAC‑CCC‑GCCCAGCTTTTTCCAGCCAAAAAACCGCCCCGCCAATCGACGGGGTTGAGGTACGCGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAgtg                       <  4:13357/136‑1 (MQ=255)                                                                                                        cGGCCTGGTGGCGCGAAAGGAGGGCGAAG‑CCC‑TCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAgtg                       <  4:144244/135‑1 (MQ=255)                                                                                                            ctggtggCGCGAAAGGAGGGCGAAG‑CCC‑TCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTg                  <  4:301106/133‑1 (MQ=255)                                                                                                                tgtCGCGAAAGGAGGGCGAAG‑CCC‑TCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCt               >  8:322990/1‑135 (MQ=255)                                                                                                                 gtCGCGAAAGGAGGGCGAAG‑CCC‑TCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCTTa             >  7:7700/1‑136 (MQ=255)                                                                                                                       aaaGGCGGGCTACG‑CCC‑GCCCAGCTTTTTCCAGCAAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCTTAcagcag       <  3:41847/136‑1 (MQ=255)                                                                                                                        aaGGAGGGCGAAG‑CCC‑TCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCTTAcagca        >  1:63651/1‑134 (MQ=255)                                                                                                                            aggGCGAAG‑CCC‑TCCCAGCTTTTTCCCGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCTTACAGCAGCATgg  <  8:310909/136‑1 (MQ=255)                                                                                                                            aggGCGAAG‑CCC‑TCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCTTACAGCAGCATgg  <  3:296673/136‑1 (MQ=255)                                                                                                                            |                                                                                                                                          GATGTCCCGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCGAAAGGAGGGCGAAG‑CCC‑TCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCTTACAGCAGCATGG  >  ACB122/399169‑399427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑