| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 5,883,454 | C→G | 19.0% | intergenic (+86/+34) | quiP → / ← metQ_2 | Acyl‑homoserine lactone acylase QuiP/putative D‑methionine‑binding lipoprotein MetQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 5,883,454 | 0 | C | G | 19.0% | 63.6 / 2.9 | 35 | intergenic (+86/+34) | quiP/metQ_2 | Acyl‑homoserine lactone acylase QuiP/putative D‑methionine‑binding lipoprotein MetQ |
| Reads supporting (aligned to +/- strand): ref base C (18/10); new base G (3/4); total (21/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTA‑CCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAA‑GGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCA > ACB122/5883320‑5883584 | gggggTCATCCGGGCCCAATGTACCTT‑CGGCGGGGACCCCCCTAAGAAAGCGCGGGCTTGGGGGCCGCTTCGCACAAAAGGGCTGCTCCGGGGGTGGGGGGGCGGGCCTCAAAAAAACCGCCGACACAA‑GGTGTCg < 4:56168/136‑1 (MQ=255) cGGCGCAAAGTACCTTACGGCTGGTA‑CCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAA‑GGTGTCGGCGTTGGcac > 5:199141/1‑135 (MQ=255) cGGCGCAAAGTACCTTACGGCTGGTA‑CCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAA‑GGTGTCGGCGGTGGcac > 1:248453/1‑135 (MQ=255) gcAAAGTACCTTACGGCTGGTA‑CCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAA‑GGTGTCGGCGTTGGCACatat > 8:281543/1‑135 (MQ=255) gTA‑CCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAA‑TGTGTCGGCGTTGGGACATATCAGGCGTTGGTTGCGaaa > 6:139252/1‑134 (MQ=255) gtgcccccGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAA‑GGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAAt > 8:197043/4‑135 (MQ=255) cccccGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTCCGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAA‑GGGGTGGGGGTTGGCCCATATGCGGCGGTGCGAGCGCAATccg > 5:317063/1‑133 (MQ=255) cccccGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAA‑GGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCa > 2:248336/1‑134 (MQ=255) ggCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAA‑CGTGTGGGCGTTGGCCCATATAAGGCCTTGCGAGCGCAATCAGAACGCCGGCAGTACg > 8:31148/1‑136 (MQ=255) ctgctTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCCCCACAA‑GGTGTCGGCGGTGGCCCCTATCAGGCGGTGCTAGCGAAATCCGCACGCCGGCAGGACGGc > 8:70714/1‑136 (MQ=255) tGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGCCACAA‑GGTGTCGGCGTTGGCCCATATCAGGCGTTGCTAGCGAAATAAGAACGCCGGCAGTACGGCAccgt > 1:357845/1‑133 (MQ=255) ccGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACCA‑GGTGTCGGCGGTGGCACCTATCAGGCGGTGCTAGCGAAATCAGAACGCCGGCAGTACGGCCCCGTgggtct > 7:234440/1‑132 (MQ=255) tCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAA‑GGTGTCGGCGGTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCCCCTTGGTACTTCTTg > 4:128582/1‑136 (MQ=255) ggCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAA‑GGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGCAATCAGAACGCCGGCAGTACGGCCCCTTGGTACTTCTTGGCaatgaatg > 2:205349/1‑135 (MQ=255) gggcggtgCGGGCAGGGCTGAAGAAAAACCCCGACACAA‑GGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATTAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTcc < 7:226874/131‑1 (MQ=255) gggcggtgCGGCCTGGGGTCAACAAACACCCCGA‑ACAATGGTGTGGGCGTTGGCACATATCAGGCGTTGCTAGCTAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTcc < 7:370875/131‑1 (MQ=255) gggCTGGGCGGGCAGGGCTGAACAAAACCGCCGACACAA‑GGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATTAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTcc < 7:232658/134‑1 (MQ=255) tgCGGGCAGGGCTGAAGAAAAACGCCGACACAA‑GGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCt > 7:115467/1‑97 (MQ=255) tgCGGGCAGGGCTGAAGAAAAACGCCGACACAA‑GGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCt < 8:115467/97‑1 (MQ=255) tgCGGGCAGGGCTGAAGAAAAACGCCGACACAA‑GGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCt > 1:246844/1‑135 (MQ=255) tgCGGGCAGGGCTGAAGAAAAACGCCGACACAA‑GGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTg > 7:297789/1‑136 (MQ=255) tgCGGGCAGGGCTGAAGAAAAACGCCGACACAA‑CGTGTCCGCGTTGGCACATATCACGGGTTGCTAGCGAAATCACAACGCCGGCAGTACGGCACCATGGTACTTCTTGGCAATGAATGCCATGACTCACGGGCt > 6:68535/1‑135 (MQ=255) gggCAGGGCTGAAGAAAAACGCCGACACAA‑GGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTTGCAATGAATGCCTTGACTTCCGGGCTgg > 5:171564/1‑134 (MQ=255) gCTGAAGAAAAACGCCGACACAA‑GGGGTCGGCGGTGGCACATATCACGCGGTTCTAGCGAAATAACCAACCAGGCACTACGGCACCGTGGTAATTCGTGGCAATGAAAGCCTAGACTTCCGGGCTGGTCAGGCCCt > 4:377670/1‑136 (MQ=255) tgcataaaaaaCGCCCACACAT‑GGTGGCGGCGTTGGCACATATCAGGCGTTGCTAGCTAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCtt < 7:31148/130‑1 (MQ=255) ttaagaaAAACGCCGACACAG‑GGTGGCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCtt < 6:171564/133‑1 (MQ=255) ttaacaaaaaCGCCGACACAA‑GGTGGCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCtt < 6:199141/130‑1 (MQ=255) gaagaaAAACGCCCACACAA‑TGTGTCGGCGTTTGCACATATCAGGCGTTTCTAGCGAAATCAGAACGCCGGCAGTACGGCCACTTGGTACTTCTTGGCAATGAATGCCTTGACGTCCGGGCTGGTCAGGGCCTTg > 2:95974/1‑135 (MQ=255) cacaaaaacCCCCCACACAT‑GGTGTGGGGGTTGGAACAAATCAGGCGTTGATAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTg < 1:248336/131‑1 (MQ=255) cgccgcCACAA‑GGTGTGGGCGGTGGGACATATCAGGCGGTGGGAGCGAAATCAGAACGCCGGCAGTACGGCCCCGTGGGTCTTCTTGGCAATGTATGGCGTGGCGTCCGGGCTGGTCCGGGGCTTGGGCAGgttg > 6:10229/1‑134 (MQ=255) cccccacacac‑GGTGGGGGGGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCt < 7:197043/131‑1 (MQ=255) gACACCG‑GGTGCGGGCGTTGGCACATATCAGGCGTTGCTAGCTAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTGTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGa < 5:68535/135‑1 (MQ=255) acaG‑GGGGTCGGCGTTGGCTCATATCAGGGGTTGATAGCGAAATCAGATCGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGc < 7:45546/136‑1 (MQ=255) aa‑GGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCt < 8:297789/136‑1 (MQ=255) ggTGCCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCa < 7:281543/136‑1 (MQ=255) | GGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTA‑CCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAA‑GGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCA > ACB122/5883320‑5883584 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |