| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 1,472,154 | T→G | 27.9% | V55G (GTG→GGG) | moaC → | Cyclic pyranopterin monophosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,472,154 | 0 | T | G | 27.9% | 68.6 / 2.4 | 45 | V55G (GTG→GGG) | moaC | Cyclic pyranopterin monophosphate synthase |
| Reads supporting (aligned to +/- strand): ref base T (15/16); new base G (7/7); total (22/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACATGGTCGACGTCACTGAAAAGGCCGTGACCGAGCGCGAGGCTACCGCCGAGGCGCGGGTGCGCATGTTGCCGCAGACCTTGCAGATGATCGTCGACGGCGAACACCCCAAGGGTGACGTGTTCGCCGTGGCGCG‑CATTGCCGGGATACAG‑GCGGCGAAAAAAACCAGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTCACCAGCGTCAAGGTCGAGCTCAGCGCCGAGGGGCAGGATGCCGTGCGCATCGTCGCCCG > ACB122/1472024‑1472286 | aaCATGGTCGACGGCACTGAAAAGCCCGTGAACGATCGCGTGGGTACCGCCGAGGCGCGGGTGCGCATGTTGCCGCAGACCTTCCAGATGATCGTCGACGGCGAACACCCCTAGGGTGACGTGTTCGCCGTGgcgc < 8:290433/136‑1 (MQ=255) cATGGTCGACGTCACTGAAACGGCCGTGACCGAGCGCGAGGCTACCGCCGAGGCGCGGGTGCGCATGTTGCCGCAGACCTTGCAGATGATCGTCGACGGCGAACACCCCAAGGGTGACGTGTTCGCCGTGgcgcg < 6:313115/135‑1 (MQ=255) tGGTCGACGTCACTGAAAAGGCCGTGACCGAGCGCGAGGCTACCGCCGAGGCGCGGGTGCGCATGTTGCCGCAGACCTTGCAGATGATCGTCGACGGGGCACACCCCAAGCGGGTGGTGGTGGCCGGGgcggggc > 2:124831/1‑131 (MQ=255) ggTCGACGTCACTGAAAAGGCCGTGACCGAGCGCGAGGCTACCGCCGAGGCGCGGGTGCGCATGTTGCCGCAGACCTTGCAGATGATCGTCGACGGCGAACACCCCAAGGGTGACGTGTTCGCCGTGGCGCG‑CAt < 1:370137/135‑1 (MQ=255) tCGACGTCACTGAAAAGGCCGTGACCGAGCGCGAGGCTACCGCCGAGGCGCGGGTGCGCATGTTGCCGCAGACCTTGCAGATGATCGTCGACGGCGAACACCCCAAGGGTGACGTGTTCGCCGTGGCGCG‑CAt < 3:5538/133‑1 (MQ=255) ggCCGTGACCGAGCGCGAGGCTACCGCCGAGGCGCGGGTGCGCATGTTGCCGCAGACCTTGCAGATGATCGTCGACGGCGAACACCCCAAGGGTGACGTGTTCGCCGTGGCGCG‑CATTGCCGGGATACAg‑gcggc < 6:255855/135‑1 (MQ=255) gTGACCGAGCGCGAGGCTACCGCCGAGGCGCGGGTGCGCATGTTGCCGCAGACCTTGCAGATGATCGTCGAGGGCGCCCACCCCAAGGGGGACGTGTTGGCCGGGGCGGG‑CCTTGGCGGGCTACAG‑gcggcgca > 1:231369/1‑132 (MQ=255) gTGACCGAGCGCGAGGCTACCGCCGAGGCGCGGGTGCGCATGTTGCCGCAGACCTTGCAGATGATCGTCGACGGCGCACACCCCCAGGGTGTCGTGTTCGCCGTGGCGCG‑CAATGCCGGGATACAG‑GCGGCGaa > 6:210455/1‑134 (MQ=255) gACCGAGCGCGAGGCTACCGCCGAGGCGCGGGTGCGCATGTTGCCGCAGACCTTGCAGATGATCGTCGACGGCGACCACCCCAAGGGTGTCGGGTTCGCCGGGGCGCG‑CCGTGGCCGGGTAAAG‑GCGGCGcaaa > 3:277868/1‑134 (MQ=255) aCCGAGCGCGAGGCTACCGCCGAGGCGCGGGTGCGCATGTTGCCGCAGACCTTGCAGATGATCGTCGACGGCGCACCCCCCCAGGGTGCCGGGGTGGCCGGGGGGCG‑GCATGGCGGGGTTCAG‑GCGGGGcaaaaa > 4:202674/1‑135 (MQ=255) cGAGCGCGAGGCTACCGCCGAGGCGCGGGTGCGCATGTTGCCGCAGACCTTGCAGATGATCGTCGACGGCGCAAACCCCAAGGGTGCCGTGTTAGCCGTGGCGCG‑CATTGCCGGGATACCG‑GCGGCGAAAAAAAcc > 7:130632/1‑136 (MQ=255) gcgcGAGGCTACCGCCGAGGCGGGGGTGCGCATGTTGCCGCAGACCTTGCAGATGATCGTCGACGGCTAACACCCCAAGGGTGACGTGTTCGCCGTGGCGCG‑CATTGCCGGGATACAG‑GGGGCGAAAAAAACCAGc < 7:197461/136‑1 (MQ=255) gcgAGGCTACCGCCGAGGCGCGGGTGCGCATGTTGCCGCAGACCTTGCAGATGATCGTCGACCGCGAAAACCCCCACGGGGGCGGGGTAGCCCGGGCCCGCCATTG‑CGGGGTGCAGCGCGGCGAAAAAAccccccc > 7:12582/1‑132 (MQ=255) aGGCTACCGCCGAGGCGGGGGGGCGCATGTGGCCGCAGACCTTGCAGTTGATCGTCGACGGCGAACACCCCAAGGGTGACGTGTTCGCGGTGGCGCG‑AATTGCCGGGATACAG‑GCGGCGAAAAAAACCAGCGAcc < 4:123225/135‑1 (MQ=255) ccgccgAGGCGCGGGTGCGCATGTTGCCGCTGACCTTGCAGAGGATCGGCGACGGCTAACACCCCAAGGGTGACGGTTTCGCCGTGGCGCG‑CATTGCCGGGATACAG‑GCGGCGAAAAAAACCCGCGACCTGAtt < 8:4545/134‑1 (MQ=255) ccgccgAGGCGCGGGTGCGCATGTGGCCGCAGACCTTGCAGAGGAGCGTCGAGGGCCAACACCCCAAGGGGGACGTGTTCGCCGTGGCGCG‑CATTGCCGGGATACAG‑GCGGCTAAAAAAACCAGCGACCTGAtt < 7:162594/134‑1 (MQ=255) aGGCGCGGGTGCGCATGTTGCCGCAGACCTTGCAGATGATCGTCGACGGCGAACACCCCAAGGGTGACGTGTTCGCCGTGGCGCG‑CATTGCCGGGATACAG‑GCGGCGAAAAAAACCAGCGACCTGATTCCGCtgtg < 7:266753/136‑1 (MQ=255) cgcAGGTGGCCGCAGACCTTGCAGATGATCGTCGACGGCGAACACCCCAAGGGGGAGGTGTGCGCGGTGGCGCG‑CTTTGCCGGGATACCG‑GCGGCTAAAAAAACCAGCGACCTGATTCCGCTGTGCCACCCGCTg < 2:215347/135‑1 (MQ=255) tggCCGCAGACCTTGCAGATGATCGGCGACGGCGAACACCCCAAGGGTGACGTGTTCGCCGGGGCGCG‑CATTGCCGGGATACAG‑GCGGCGAAAAAAACCAGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTc < 6:294298/133‑1 (MQ=255) gCCGCAGACCTTGCAGATGATCGTCGACGGCGCACACCCCAAGGGTGGCGTGTTAGCCGGGGCGCG‑CCTTTGCGGGATACAG‑GCGGCGCAAAAAACCACCGACCTGATTACGCTGTGCCCCCCCCGGCTGGTCAc > 6:164149/1‑135 (MQ=255) gCCGCAGACCTTGCAGATGATCGTCGACGGCGAACACCCCAAGGGTGACGTGTTCGCCGTGGCGCG‑CATGGCCGGGATACAG‑GCGGCGAAAAAAACCAGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTCAc < 8:56940/135‑1 (MQ=255) gCAGACCTTGCATATGCGCGTCGCGGGCGAACACCCAAGGGGTGAGGGGTTCGCGGGGGCGCG‑CATTGCCGGGAACCTG‑GCGGCTAAAAAAACCAGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTCACCAg < 6:273531/135‑1 (MQ=255) aGATGATCGTCGACGGCGAACACCCCCACGGTGTCGTGTTAGGCGGGGCGCG‑CCGTGCCGGGCTACCG‑GCGGCGCAAAAAAACAGCGACCTGATTCAGCTGTGCCCCCCCCGGATGCGCACCCGCGGCAAGGTCg > 2:134941/1‑135 (MQ=255) tcggcgCCGGCGAACCCCCCCCGGGTGCGGGGGGCGCCGGGGCGCG‑CTTGGCCGGGATACCG‑GCGGCGAAAAACCCCCGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTCACCAGCGTCAAGGTCGAGCTCa < 3:104878/135‑1 (MQ=255) gtcgCCGGCTAACACCCCAAGGGTGAGGTGTGCGCCGGGGCGCG‑CATTGCCGGGATACAG‑GCGGCGAAAAAACCCAGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTCACCAGCGTCAAGGTCGAGCTCAgcg < 8:201705/136‑1 (MQ=255) cccccGGCCAACACCCCAGGGGGGCCGTGTCCGCGGTGGCGCG‑CCTTGCCGGCAAACAC‑GCGCCTAAAAAACCCAGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTCACCAGCGTCAAGGTCGAGCTCAgcg < 1:358937/131‑1 (MQ=255) aaCCCCCCACGGGTGACGTGTGCGCCGGGGCGCG‑CATGGCCGGGACACCG‑GCGGCTAAAAAAACCCGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTCACCAGCGTCAAGGTCGAGCTCAGCGCCGAGGGGc < 3:122551/135‑1 (MQ=255) aaCACCCCATGGGTGACGGGTGCGCCGTGGCGCG‑CATTGCCGGCATACAG‑GCGGCGAAAAAAACCAGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTCACCAGCGTCAAGGTCGAGCTCAGCGCCGAGGGGc < 3:122550/135‑1 (MQ=255) cacCCCACGGGTGACGGGGTCGCCGTGGCGCG‑CATTGCCGGGATACCG‑GCGGCGAAAAAACCCCGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTCACCAGCGTCAAGGTCGAGCTCAGCGCCGAGGGGCAg < 2:231369/135‑1 (MQ=255) aaGGGTGACGTGTTCGCCGTGGCGCG‑CATTGCCGGGATACAG‑GCGGCGAAAAAAACCAGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTCACCAGCGTCAAGGTCGAGCTCAGCGCCGAGGGGCAGGATGcc > 4:42315/1‑135 (MQ=255) gggTGACGTGTTCGCCGTGGCGCG‑CATTGCCGGGATACAG‑GCGGCGAAAAAAACCAGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTCACCAGCGTCAAGGTCGAGCTCAGCGCCGAGGGGCAGGATGCCGt > 6:1778/1‑135 (MQ=255) tgtTCGCCGTGGCGCG‑CATTGCCGGGATACAG‑GCGGCGAAAAAAACCAGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTCACCAGCGTCAAGGTCGAGCTCAGCGCCGAGGGGCAGGATGCCGTGCGCAtcgt > 7:369931/1‑136 (MQ=255) tgtTCGCCGTGGCGCG‑CATTGCCGGGATACAG‑GCGGCGAAAAAAACCAGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTCACCAGCGTCAAGGTCGAGCTCAGCGCCGAGGGGCAGGATGCCGTGCGCAtcgt > 7:125931/1‑136 (MQ=255) tgtTCGCCGTGGCGCG‑CATTGCCGGGATACAG‑GCGGCGAAAAAAACCAGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTCACCAGCGTCAAGGTCGAGCTCAGCGCCGAGGGGCAGGATGCCGTGCGCAtcg > 3:106696/1‑135 (MQ=255) tgtTCGCCGTGGCGCG‑CATTGCCGGGATACAG‑GCGGCGAAAAAAACCAGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTCACCAGCGTCAAGGTCGAGCTCAGCGCCGAGGGGCAGGATGCCGTGCGCAtcg > 2:178720/1‑135 (MQ=255) tgtTCGCCGTGGCGCG‑CATTGCCGGGATACAG‑GCGGCGAAAAAAACCAGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTCACCAGCGTCAAGGTCGAGCTCAGCGCCGAGGGGCAGGATGCCGTGCGCAtcg > 3:189256/1‑135 (MQ=255) tgtTCGCCGTGGCGCG‑CATTGCCGGGATACAG‑GCGGCGAAAAAAACCAGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTCACCAGCGTCAAGGTCGAGCTCAGCGCCGAGGGGCAGGATGCCGTGCGCAtcg > 5:128698/1‑135 (MQ=255) tgtTCGCCGTGGCGCG‑CATTGCCGGGATACAG‑GCGGCGAAAAAAACCAGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTCACCAGCGTCAAGGTCGAGCTCAGCGCCGAGGGGCAGGATGCCGTGCGCAtcg > 1:188699/1‑135 (MQ=255) tgtTCGCCGTGGCGCG‑CATTGCCGGGATACAG‑GCGGCG‑AAAAAACCAGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTCACCAGCGTCAAGGTCGAGCTCAGCGCCGAGGGGCAGGATGCCGTGCGCAtcgt > 6:344919/1‑135 (MQ=255) tgtTCGCCGTGGCGCG‑CATTGCCGGGATAAAG‑GCGGCTAAAAAAACCAGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTCGCCAGCGTCAAGGTCGAGCTCAGCGCCGAGGGGCAGGATGCCGTGCGCAtctt > 7:354937/1‑134 (MQ=255) gtgcGCCGGGGCGCG‑CATTGCCGGGATACAG‑GCGGCGAAAAAAACCAGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTCACCAGCGTCAAGGTCGAGCTCAGCGCCGAGGGGCAGGATGCCGTGCGCAtcgtc < 8:189235/133‑1 (MQ=255) ttCGCCGTGGCGCG‑CATTGCCGGGATACAG‑GCGGCGAAAAAAACCAGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTCACCAGCGTCAAGGTCGAGCTCAGCGCCGAGGGGCAGGATGCCGTGCGCAtcgtc < 8:369931/135‑1 (MQ=255) gcggCCGGGGCGCG‑CTTGGCCGGCAACCCG‑GGGGCTAAAAACCCCCGCGACCTGATTCCGCTGGGCCACCCGCTGATGCTCACCAGCGTCAAGGTCGAGCTCAGCGCCGAGGGGCAGGATGCCGTGCGCAtcgtc < 5:379024/132‑1 (MQ=255) gCCGTGGCGCG‑CATTGCCGGGATACAG‑GCGGCGAAAAAAACCAGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTCACCAGCGTCAAGGTCGAGCTCAGCGACGAGGGGCAGGATGCCGTGCGCATCGTCGccc > 8:148918/1‑136 (MQ=255) cGTGGCGCG‑CATTGCCGGGATACAG‑GCGGCGAAAAAAACCAGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTCACCAGCGTCAAGGTCGAGCTCAGCGCCGAGGGGCAGGATGCCGTGCGCATCGTCGCCCg > 5:173197/1‑135 (MQ=255) | AACATGGTCGACGTCACTGAAAAGGCCGTGACCGAGCGCGAGGCTACCGCCGAGGCGCGGGTGCGCATGTTGCCGCAGACCTTGCAGATGATCGTCGACGGCGAACACCCCAAGGGTGACGTGTTCGCCGTGGCGCG‑CATTGCCGGGATACAG‑GCGGCGAAAAAAACCAGCGACCTGATTCCGCTGTGCCACCCGCTGATGCTCACCAGCGTCAAGGTCGAGCTCAGCGCCGAGGGGCAGGATGCCGTGCGCATCGTCGCCCG > ACB122/1472024‑1472286 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |