Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	3,047,211	T→G	21.6%	K124N (AAA→AAC)	CJ019_02862 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	3,047,211	0	T	G	21.6%	97.0 / 5.5	58	K124N (AAA→AAC)	CJ019_02862	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (6/38);  new base G (12/2);  total (18/40)
Fisher's exact test for biased strand distribution p-value = 1.46e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTTCGCCGTCGCCGGTGTTGGCCAGCCACAGGCTGCCAGGGAGGTTGCTATGCGGCTGGTCTTCTATGAAGCGACCTTGCAGCCAT  >  ACB122/3047077‑3047340                                                                                                                                        |                                                                                                                                   gCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAACCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCt                                                                                                                                    <  4:256812/135‑1 (MQ=255)  cATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCttt                                                                                                                                  <  7:169998/136‑1 (MQ=255)         gCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAg                                                                                                                            <  5:242078/135‑1 (MQ=255)              cgcgGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTAc                                                                                                                       <  1:196714/135‑1 (MQ=255)              cgcgGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTAc                                                                                                                       <  4:58608/135‑1 (MQ=255)              cgcgGTACCAGTACAGGTTGGTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTAc                                                                                                                       <  1:99229/135‑1 (MQ=255)              cgcgGTACCAGTACAGGTGGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAAGCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTAc                                                                                                                       <  5:245066/135‑1 (MQ=255)                cgGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCGTTTTGGACGTTAAGCa                                                                                                                    >  7:138432/1‑136 (MQ=255)                 ggTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCAc                                                                                                                   >  8:16869/1‑136 (MQ=255)                 ggTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCGTTTTGCAGGTTACGCa                                                                                                                    >  4:117956/1‑135 (MQ=255)                      cAGTAGAGGTTGTTATAGCCCAAGGCGTTCGCGCGTTTTACCGCGTTCCAGCTTAACCACCACTCGGAGCGGCAGTAAAAGACCAGTGCTTGGGCCAGGGGCCCGGCCGT‑GGCGTTTTGCAGGTTACGCACGAag                                                                                                               <  4:257830/135‑1 (MQ=255)                       aGTACCGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTCACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGGCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAagta                                                                                                             <  7:250959/136‑1 (MQ=255)                        ggaCAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAagta                                                                                                             <  3:139093/133‑1 (MQ=255)                            aGGTTGTTATAGCCCTAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCt                                                                                                        <  7:84705/136‑1 (MQ=255)                             ggTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCt                                                                                                        <  4:122511/135‑1 (MQ=255)                                tgttATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGcc                                                                                                     <  4:391992/135‑1 (MQ=255)                                      aGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGACCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGGGTTTTTGAGGTTTCTCCCGAAGTAGGCCGGCCCG‑GCCg                                                                                              >  3:140617/1‑135 (MQ=255)                                          cacggCTTTCGCGCGTGTTACCGCGTTCCAGCTTACCCAGCAAGCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCt                                                                                          <  5:373572/132‑1 (MQ=255)                                          cAAGGGTTTGGCGGGTTTTACCGCGTTCCAGCTTAACCATCAATCGCAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTc                                                                                         <  7:338206/136‑1 (MQ=255)                                           aaGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGGTTTTTGCAGGGTACGCACGAAGTAGTCCTGCCAT‑GCCGGGc                                                                                           >  2:101353/1‑133 (MQ=255)                                           aaGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGGTTTTTGAAGGGTTCGGCCGCAGAAGTACTGCCATGGCCggggc                                                                                          >  6:106452/1‑133 (MQ=255)                                           aaGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCGTTTTTGAGGTTAAGCACCCAGTAGTCCAGCCCT‑GCCgggg                                                                                           >  5:159974/1‑132 (MQ=255)                                           aaGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCGTTTTGGAGGTTAAGCACGAAGTAGTGCTGCCAA‑GCCGGGc                                                                                           >  2:30448/1‑133 (MQ=255)                                           aaGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCGTGTTGCAGGGTACTCCCGCAGTATTGCTGGCAT‑GCCGGGcg                                                                                          >  7:287296/1‑133 (MQ=255)                                            aGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGGGTTTTGTGGTTTTGGGACGCAGTATTGATGGCAT‑GCCGGGcggg                                                                                        >  1:361640/1‑132 (MQ=255)                                               cTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGGGGTTTGGAGGTTTAGGCCGAAGTAGTGCTGGCAT‑GCCGGGCTcgggtg                                                                                    >  7:386400/1‑131 (MQ=255)                                               cTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTTGAGGGTTCGAACGAAGTTGTCCTGCCCA‑GCCGGGCTCAGTtc                                                                                    >  8:64080/1‑136 (MQ=255)                                               cTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTtc                                                                                    <  8:13831/136‑1 (MQ=255)                                               cTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCGGTTTTGAGGTTTAGCACCAACTAGTGCGGCCAG‑GCCCGGCGCCGttg                                                                                    >  7:200124/1‑135 (MQ=255)                                                     cgcgTTTTCCCGCGTACCAGCTAAACCACCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTtcgccgt                                                                               <  5:260947/135‑1 (MQ=255)                                                     cgcgTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGTGGGGGTTTTGC‑GGTTTCGCGCGCAGTTGTGCTGCCCT‑GCCCGGGGGAGTtcggcgt                                                                               >  5:92086/1‑135 (MQ=255)                                                     cgcgTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGGTTTTTTGAGGTTACGCCCGAAGTAGTGCTGCCCA‑GCCCGGCGCACTtagccgt                                                                               >  4:342075/1‑135 (MQ=255)                                                     cgcgTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGGGGTTTGGTGGTTTCGCACGAAGTTGTGCGTCCAT‑GCCGGGCGCCGTtggccggg                                                                              >  7:294408/1‑134 (MQ=255)                                                     cgcgGTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTtcgccgt                                                                               <  2:214638/135‑1 (MQ=255)                                                               cgcgTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTTCGCCGTCGCCGGTGtt                                                                     <  2:246064/135‑1 (MQ=255)                                                               cgcgTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTTCGCCGTCGCCGGTGtt                                                                     <  3:342075/135‑1 (MQ=255)                                                                       aGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTTCGCCGTCGCCGGTGTTGgccagcc                                                             <  5:350664/135‑1 (MQ=255)                                                                       aGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTTCGCCGTCGCCGGTGTTGgccagcc                                                             <  1:83/135‑1 (MQ=255)                                                                       aGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTTCGCCGTCGCCGGTGTTGgccagcc                                                             <  6:324545/135‑1 (MQ=255)                                                                          ttAACCCGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTTCGCCGTCGCCGGTGTTGGCCAGCcaca                                                          <  1:305314/135‑1 (MQ=255)                                                                               cAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTTCGCCGTCGCCGGTGTTGGCCAGCCACAGGCTGc                                                    <  7:61032/136‑1 (MQ=255)                                                                                aGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCtttt                                                                                                                                 <  6:221349/59‑1 (MQ=255)                                                                                aGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCgttt                                                                                                                                 >  5:221349/1‑59 (MQ=255)                                                                                   aaTCGGAGCGGCAGTACAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCGCAGTTCGCCGTCGCCGGTGTTGGCCAGCCACAGGCTGCCAg                                                 <  3:5674/135‑1 (MQ=255)                                                                                     tCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGGTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTTCGCCGTCGCCGGTGTTGGCCAGCCACAGGCTGCCAggg                                               <  4:375563/135‑1 (MQ=255)                                                                                      cGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTTCGCCGTCGCCGGTGTTGGCCAGCCACAGGCTGCCAGGGa                                              <  1:332478/135‑1 (MQ=255)                                                                                      cGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCGCAGTTCGCCGTCGCCGGTGTTGGCCAGCCACAGGCTGCCAGGGa                                              <  3:61160/135‑1 (MQ=255)                                                                                                 tAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTTCGCCGTCGCCGGTGTTGGCCAGCCACAGGCTGCCAGGGAGGTTGCTATGc                                   <  6:117361/135‑1 (MQ=255)                                                                                                 tAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTTCGCCGTCGCCGGTGTTGGCCAGCCACAGGCTGCCAGGGAGGTTGCTATGc                                   <  6:92086/135‑1 (MQ=255)                                                                                                   aaaGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTTCGCCGTCGCCGGTGTTGGCCAGCCACAGGCTGCCAGGGAGGTTGCTATGCgg                                 <  8:200124/135‑1 (MQ=255)                                                                                                   aaaGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCGTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTTCGCCGTCGCCGGTGTTGGCCAGCCACAGGCTGCCAGGGAGGTTGCTATGCgg                                 <  7:9179/135‑1 (MQ=255)                                                                                                    aaGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTTCGCCGTCGCCGGTGTTGGCCAGCCACAGGCTGCCAGGGAGGTTGCTATGCGGCt                               <  7:24466/136‑1 (MQ=255)                                                                                                    aaGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTTCGCCGTCGCCGGTGTTGGCCAGCCACAGGCTGCCAGGGAGGTTGCTATGCGGCt                               <  8:240005/136‑1 (MQ=255)                                                                                                    aaGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTTCGCCGTCGCCGGTGTTGGCCAGCCACAGGCTGCCAGGGAGGTTGCTATGCGGCt                               <  7:16869/136‑1 (MQ=255)                                                                                                     aGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTTCGCCGTCGCCGGTGTTGGCCAGCCACAGGCTGCCAGGGAGGTTGCTATGCGGCt                               <  6:159974/135‑1 (MQ=255)                                                                                                     aGACCAGTAGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTTCGCCGGCGCCGGTGTTGGCCAGCCACAGGCTGCCAGGGAGGTTGCTATGCGGCt                               <  1:393337/135‑1 (MQ=255)                                                                                                              gttggtCCAGGTGCCCGGCCGT‑GGGGTTTTGGAGGTTTCGCACGAAGTAGTCCTGCCAT‑TCCGGGCGCAGTTCGCCGGCGCCGGTGTTGGCCCGCCCCCGGCTGCCCGGGAGGTTGTTATGCGGCTGGTCTTCTAt                     >  8:131710/1‑136 (MQ=255)                                                                                                                tggtCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTTCGCCGGCGCCGGTGTTGGCCAGCCACAGGCTGCCAGGGAGGTTGCTATGCGGCTGGTCTTCTATg                    <  5:273165/135‑1 (MQ=255)                                                                                                                     cAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTTCGCCGTCGCCGGTGTTGGCCAGCCACAGGCTGCCAGGGAGGTTGCTATGCGGCTGGTCTTCTATGAAGCGa              <  8:287296/136‑1 (MQ=255)                                                                                                                                ccGT‑GGGTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTTCGCCGTCGCCGGTGTTGGCCAGCCACAGGCTGCCAGGGAGGTTGCTATGCGGCTGGTCTTCTATGAAGCGACCTTGCAGcc    >  8:271655/1‑135 (MQ=255)                                                                                                                                  gT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTTCGCCGTCGCCGGTGTTGGCCAGCCACAGGCTGCCAGGGAGGTTGCTATGCGGCTGGTCTTCTATGAAGCGACCTTGCAGCCAt  <  3:185495/135‑1 (MQ=255)                                                                                                                                  gT‑GGCTTTTTGCAGGTTACGCACGAAGTAGGCCTGCCAT‑GCCGGGCTCAGTGCGCCGTCGCCGGTGTTGGCCAGCCACAGGCTGCCAGGGAGGTTGCTATCCGGCTGGTCTTCTATGAAGCGACCTTGCAGCCAt  <  5:106452/135‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   GCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGTAAAAGACCAGTGGTTGGTCCAGGTGCCCGGCCGT‑GGCTTTTTGCAGGTTACGCACGAAGTAGTCCTGCCAT‑GCCGGGCTCAGTTCGCCGTCGCCGGTGTTGGCCAGCCACAGGCTGCCAGGGAGGTTGCTATGCGGCTGGTCTTCTATGAAGCGACCTTGCAGCCAT  >  ACB122/3047077‑3047340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑