Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	399,292	A→C	35.6%	intergenic (‑186/+122)	CJ019_00365 ← / ← aceF	hypothetical protein/Dihydrolipoyllysine‑residue acetyltransferasecomponent of pyruvate dehydrogenase complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	399,292	0	A	C	35.6%	48.5 / 8.6	66	intergenic (‑186/+122)	CJ019_00365/aceF	hypothetical protein/Dihydrolipoyllysine‑residue acetyltransferasecomponent of pyruvate dehydrogenase complex
Reads supporting (aligned to +/- strand):  ref base A (22/18);  new base C (7/14);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCATGATGTCCCGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCTTACAGCA  >  ACB122/399164‑399421                                                                                                                                  |                                                                                                                                     tGCATGATGTCCCGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGTCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGGTTTTCTGCCATTTTTCGC‑GCAAGGAggggg                                                                                                                                 >  7:259482/1‑132 (MQ=255)  gCATGATGTCCCGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTTCGGGCTTGTGTAGCGGAAAGGGGGGGGa                                                                                                                                >  1:180796/1‑135 (MQ=255)      gatgTCCCGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GGAAGGGGGGCGCAGccc                                                                                                                           >  5:116161/1‑135 (MQ=255)      gatgTCCCGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GCAAGGCGGGCGCAGccc                                                                                                                           >  2:239700/1‑135 (MQ=255)      gatgTCCCGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGCGGGCGAAGccc                                                                                                                           >  5:346120/1‑135 (MQ=255)      gatgTCCCGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCt                                                                                                                          >  7:321142/1‑136 (MQ=255)      gatgTCCCGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCt                                                                                                                          >  7:256905/1‑136 (MQ=255)             cGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCC‑GC‑t                                                                                                                 >  7:328463/1‑136 (MQ=255)             cGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCA‑GC‑t                                                                                                                 >  7:276093/1‑136 (MQ=255)              gcgggCTGGCAAGGGGCCATGCTTGAGGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCA‑GC‑tt                                                                                                                <  8:372779/134‑1 (MQ=255)               aGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCA‑GC‑ttt                                                                                                               <  8:123160/136‑1 (MQ=255)                gggCTGGCATGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCGGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGGCGC‑GAAAGGAGGGCGAAGCCCTCCCA‑GC‑tttt                                                                                                              <  7:10140/136‑1 (MQ=255)                gggCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCA‑GC‑tttt                                                                                                              <  7:16060/136‑1 (MQ=255)                       cAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCA‑GC‑TTTTTCCAGGc                                                                                                       >  7:94529/1‑136 (MQ=255)                       cAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCATTGCGGCCTTGTGTCGC‑GAAAGGCGGGCGAAGCCCTCCCA‑GC‑ATTTTCCac                                                                                                         >  4:272127/1‑133 (MQ=255)                              ccATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGAGGGCGCAGCCCTCCCA‑GC‑TTTTTCCAGGCaaaaa                                                                                                  >  4:223612/1‑134 (MQ=255)                              ccATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCA‑GC‑TTTTTCCAGGCaaaaaa                                                                                                 >  8:262144/1‑135 (MQ=255)                                   cTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCA‑GC‑GTTTTGCAGGCaaaaaaacccc                                                                                            >  1:58557/1‑131 (MQ=255)                                    ttgttgCGGTGGACAGGTTAGCGGAGGACGTGGCCGATTCTGCAGTTTGGCTGTAGATTTTGGGGCCGCTTTGCGGCCTGGTGGCGC‑TAAAGGAGGGCGAAGCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑Gcc                                                                                           <  6:144981/134‑1 (MQ=255)                                          gtggggACTGGTTTTGGGCTGCGGTGGCCTATTCTGCTGTTTGCCGGTCTATTTGGGGGCCGCTTTGGGGCGTTGGGGCGG‑GAAAGGAGGGCGAAGCCCGCCAA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAAt                                                                                   <  8:237782/132‑1 (MQ=255)                                           tgtgGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTAGGGGCCGCTTTGCGGCCATGTGTCGC‑GAAAGGCGCGCGCAACCCTCCCC‑AC‑ATTTTGCAGGCAAAAAAAA‑ACcccccccac                                                                                   >  6:339124/1‑132 (MQ=255)                                                aCAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GACAGGAGGGCGGAGCCCGCCCC‑GC‑GTGTTGCCGGCAAAAAAAA‑GCCCCGCCCATCGACg                                                                              >  5:94816/1‑135 (MQ=255)                                                aCAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGAGGGCGAACCCCTCCCA‑CC‑GTTTTACAGGCAAAAAAAA‑GCCCCGCCAATCGCCg                                                                              >  5:114994/1‑135 (MQ=255)                                                 cAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACggg                                                                            <  8:94529/136‑1 (MQ=255)                                                       aTCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGCGGGCGAAGCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACGGGGGTGa                                                                       >  3:198241/1‑135 (MQ=255)                                                       aTCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCC‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACGGGGGTGa                                                                       >  3:120063/1‑135 (MQ=255)                                                       aTCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCGTTGCGGGCTTGTGTCGC‑GGAAGGAGGGCGCAGCCCTCCCA‑GC‑TTTTTGCAGGGAAAAAAAA‑GCCCCGCCCATCGCCGGGGGTgc                                                                       >  1:127313/1‑134 (MQ=255)                                                        tCGGCTGTCGGGGCCTATTGTGCTGTTTGGCTGGTGTTTTTGGGGCCGCTTTGCGGGCTTGTGTGGC‑GAACGGAGGCCTACGCCCCCCCA‑GG‑TTTTTCCAGCCAAAAAAAC‑GCCCCGCAAATCGACGGGGTTGa                                                                       <  6:268467/134‑1 (MQ=255)                                                        tCGGCTGACGTGGCCGTTTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCTTGGTGTCGC‑TAACGGCGGGCTAAGCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACGGGGTTGa                                                                       <  4:313800/134‑1 (MQ=255)                                                          ggCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GGCACGGGGGGGCAGGCCGCCCA‑GCGTTTTTGCGGGCAAAAAAAC‑GCCCCCCCAA‑CGGCgggggggtg                                                                      >  5:335350/1‑128 (MQ=255)                                                          ggCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GCAAGGCGGGCGAAGCCCTCCCA‑GC‑GTTTTGCAGGCAAAAAAAA‑GCCCCGCCAATCGCCGGGGGTGAgg                                                                     >  2:132939/1‑134 (MQ=255)                                                          ggCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACGGGGTTGAgg                                                                     <  6:98176/134‑1 (MQ=255)                                                            cTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGAGGGCGCAGCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACGGGGGTGAGGTACg                                                                 >  7:77345/1‑136 (MQ=255)                                                                 gTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GGAAGGCGGGCGAAGCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCCATCGACGGGGTTGAGGTAagagag                                                             >  6:166931/1‑133 (MQ=255)                                                                 gTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTGCCA‑GC‑TTTTTCCCGGCAAAAAAAA‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGt                                                            >  8:65056/1‑136 (MQ=255)                                                                 gTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGt                                                            >  7:121987/1‑136 (MQ=255)                                                                  tGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGAGGGCGCAGCCCTCCCC‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTg                                                           >  8:307876/1‑136 (MQ=255)                                                                    gCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGc                                                         <  8:321142/136‑1 (MQ=255)                                                                            tGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGCGGGCGAAGCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGa                                                  <  3:202817/135‑1 (MQ=255)                                                                            tGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCg                                                   >  4:202817/1‑134 (MQ=255)                                                                             gCAGTTGGGCGGGACTTTTGGGGGCCGCTTTGCGGCCTGGTGGCCC‑CAACGGCGGCCGAACCCCTCCCA‑GC‑TTTTTCCCGGCAAAAAAAA‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGa                                                  <  3:65001/134‑1 (MQ=255)                                                                                    ggCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGAGGGCGCAGCCCTCCCA‑CC‑TTTTTCCAGGCAAAAAAAA‑CCCCCCCCAAACGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTAc                                         >  7:57851/1‑136 (MQ=255)                                                                                     tgggtagaTTTTGGGGCGGCTTGGGGGCTGTTGGCCGT‑GAACGCGGGGCTAAGCCAGCCCT‑GC‑TTTTTCCTGCCAAAAAAAA‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACt                                        <  8:168908/131‑1 (MQ=255)                                                                                     gggggAGATTTGGGGGCCGCTTTGGGGCCGTGTGACGC‑GAACGGCGGGCTAAGCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTAc                                         <  5:187998/132‑1 (MQ=255)                                                                                     gCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGGGTCGC‑GAAAGGAGGGCGAAGCCCGCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTAc                                         <  4:187980/135‑1 (MQ=255)                                                                                      cTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACGGGGGTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTg                                       >  7:240433/1‑136 (MQ=255)                                                                                          cgaTTTTGGGGCGGCTTTGCGGCGTGGTGGCGC‑TAAAGGAGGGCGAAGCCCGCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGcccc                                   <  8:77345/135‑1 (MQ=255)                                                                                            aTTTTGGGGCCGGTTGGCGGCCTTGGGGCGC‑GAACGGAGGGCGACGCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTc                                 <  7:50843/136‑1 (MQ=255)                                                                                                gggggCCGCTTTGCGGCCGTGTGGCGC‑GAAAGGAGGGCGAAGCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGc                               <  2:248658/133‑1 (MQ=255)                                                                                                 ggggCCGCTTGGGGGCGGTGGGGCGC‑CAATGGCGGCCCACCCCCCCCCA‑GC‑TTTTTCCAGCCAAAAAAAC‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAag                            <  7:17666/136‑1 (MQ=255)                                                                                                 ggggCCGCTTGGCGGCGGGGGGGCCC‑GAACGCAGGCCCACCCCCCCCCA‑GC‑TTTTTCCAGCCAAAAAAAC‑CCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCa                              <  2:127313/134‑1 (MQ=255)                                                                                                  gggCGGTTTTGGGGCTGTGTGTCG‑‑GAAAGCGGGTCGAACGCCACCCATGG‑TTTTTCAAGCAAAAAAAAA‑GCCCCGCAAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAag                            <  2:380406/135‑1 (MQ=255)                                                                                                      cGCTTTGCGGCCTTGTGTCGC‑GAAAGGCGGGCGAAGCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAggg                       <  8:192745/136‑1 (MQ=255)                                                                                                      cGCTTGGGGGCCTGGTGTCGC‑CAACGGCGGGCGAACCCCCCCCA‑GC‑TTTTTCCAGCCAAAAAAAA‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAaggagg                        <  6:116161/135‑1 (MQ=255)                                                                                                       gCTTTGGGGCCGTGGGGGCC‑GAACGGCGGGCGCAGCCCGCCCA‑GC‑TTTTTCCAGGCAAAAAAAACGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAggg                       <  8:166240/136‑1 (MQ=255)                                                                                                       gCTTTGCGGCCTTGTGTCGC‑GAAAGGCGGGCGAAGCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAggg                       <  4:31448/135‑1 (MQ=255)                                                                                                       gCTTTGCGGCCTTGTGTCGC‑GAAAGGCGGGCGAAGCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAggg                       <  6:346120/135‑1 (MQ=255)                                                                                                           tGCGGCCTGGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAgt                  <  8:374306/136‑1 (MQ=255)                                                                                                            gcggcgTGGTGTCGC‑GAACGGCGGGCGACCCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAC‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAgtg                 <  8:240433/136‑1 (MQ=255)                                                                                                             gggCCTTGGGCCGC‑CAACGCCGGGCGACGCCCCCCCA‑GC‑TTTTTCCAGGCAAAAAAAC‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAgtg                 <  5:372374/134‑1 (MQ=255)                                                                                                             cGGCCTGGGGGCGC‑CAAAGGCCGGCCAACCCCCCCCA‑GC‑CTTTTCCCCGCCAAAAAAA‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAgt                  <  2:58557/134‑1 (MQ=255)                                                                                                                 ctggtggCGC‑GAACGGCGGGCGACGCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAC‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTg            <  7:287937/133‑1 (MQ=255)                                                                                                                     tgtCGC‑GAAAGGAGGGCGAAGCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCt         >  8:349895/1‑135 (MQ=255)                                                                                                                             aaaGGAGGGCGAAGCCCTCCCA‑GC‑TTTTTCCAGGAAAAAAAAA‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCTTAcagca  <  6:286149/135‑1 (MQ=255)                                                                                                                              aaGGAGGGCGAAGCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACgg                                                                             <  6:314327/59‑1 (MQ=255)                                                                                                                              aaGGAGGGCGAAGCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACgg                                                                             >  5:314327/1‑59 (MQ=255)                                                                                                                                  |                                                                                                                                     TGCATGATGTCCCGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCA‑GC‑TTTTTCCAGGCAAAAAAAA‑GCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCTTACAGCA  >  ACB122/399164‑399421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑