Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB122	5,756,060	(A)6→5	coding (781/834 nt)	CJ019_05400 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	5,756,055	0	A	.	96.8%	267.1 / 3.3	65	coding (776/834 nt)	CJ019_05400	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (47/13);  minor base C (0/2);  total (47/18)
Fisher's exact test for biased strand distribution p-value = 5.55e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGC  >  ACB122/5755946‑5756183                                                                                                               |                                                                                                                                 cAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAACACGCCGCCAGCAGGAAGCGCTg                                                                                                        >  6:153636/1‑135 (MQ=255) cAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTg                                                                                                        >  1:195275/1‑135 (MQ=255)      cGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgc                                                                                                   >  3:414048/1‑135 (MQ=255)      cGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgc                                                                                                   >  3:362277/1‑135 (MQ=255)       gACCTGGCCAAGGTCTTGAAGACCATCGAGGCAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgcc                                                                                                  >  5:80277/1‑135 (MQ=255)       gACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgcc                                                                                                  >  3:504408/1‑135 (MQ=255)        cccggcccAAGGTCTTCAAGCCCACCCCGCAACCCCGCCCCCGCCCGGCCCGGCACGCTCACGCGCCCCCCAAGACGCCCCTGCGGCCG‑CAGCAGGACGC‑GCAAAAACGCCGCCAGCAGGAAGCGCTGgccgccg                                                                                                 <  2:195275/129‑1 (MQ=255)        aCCTCGCCAAGGTCTTCAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTCAACCTGAAGAGGCACGCAAGCAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgccg                                                                                                 <  7:405652/135‑1 (MQ=255)           tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAACCGCCGCCAGCAGGAAGCGCTGGccgccgccg                                                                                              >  3:40820/1‑135 (MQ=255)           tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGccgccgccg                                                                                              >  1:306821/1‑135 (MQ=255)           tGGCCAACGTCTTGAAGACCATCGAGGATACCCTCGCCCGCCAGGACCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCAGCTGGCG‑CTGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGccgccgccg                                                                                              >  2:68933/1‑135 (MQ=255)            ggCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCcgccgccgc                                                                                             >  5:519060/1‑135 (MQ=255)                aaGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCCAGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAACCGCCGCCAGCAGGACGCGCTGGCCGCCGCCGCTGcc                                                                                         >  8:239798/1‑135 (MQ=255)                 aGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGccc                                                                                        >  6:174462/1‑135 (MQ=255)                   gTCTGGAACCCCAGCGCGGCACCCCCCGCCCGCCAGGCCCGGGACGCGGAAGAGCCACGCAAGAAGCCCCTGCGGCCG‑CAGCAGGACGC‑GTAAAAACCCCGCCAGCAGGAAGCGCGGGCCGCCGCCGCTGCCcgc                                                                                      <  4:414048/135‑1 (MQ=255)                    tcgagAACACCAGCGAGGAACCCCGCGCCCGACCGGCCGGTTTCGCTGACGAGGCCCGCAAGAGGGCCCTGCTGCCG‑CAGCAGTAAG‑GGGAAACACGCCGCCCGCAGGAAGCGCTGGCCGCCGCCGCTGCCcgcg                                                                                     <  8:391916/131‑1 (MQ=255)                    tCTTGAAGACCATCGAGCAACCCCTCGCCCGCCAGGCCCGTGACGCTGAAGCGCCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCcgc                                                                                      <  4:362277/134‑1 (MQ=255)                     cTTGAAGCCCAACGAGCAACCCCCCGCCCGCCAGGCCCGTCAAGCGTACGAGGCACGCACCAAGCCCCTGCGGGCG‑CCGCAGGAAGCGG‑AAACACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGa                                                                                    <  6:80277/135‑1 (MQ=255)                     cTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGGAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGa                                                                                    >  6:228461/1‑135 (MQ=255)                     cTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCTGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGa                                                                                    >  4:216975/1‑135 (MQ=255)                          aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCggg                                                                               >  2:328994/1‑135 (MQ=255)                          aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCggg                                                                               >  1:319394/1‑135 (MQ=255)                          aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCggg                                                                               >  4:138927/1‑135 (MQ=255)                          aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCggg                                                                               >  7:541759/1‑135 (MQ=255)                           gACCATCGAGGAACCCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGc                                                                              <  4:436367/135‑1 (MQ=255)                                 cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGa                                                                        >  4:1196/1‑135 (MQ=255)                                 cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGa                                                                        >  8:64900/1‑135 (MQ=255)                                 cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGa                                                                        >  1:83079/1‑135 (MQ=255)                                   aGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTgcgc                                                                      >  6:10354/1‑135 (MQ=255)                                     cacaCCCGCGCCCGCCCGGCCCGTGACGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGACGC‑GCAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCt                                                                    <  7:293042/132‑1 (MQ=255)                                       ccccccCGCCGGCCCGCCCCGTGACCCTCACCCGGCACCCACGACGCCCCTGGGGGCG‑CCGCCGCAAGCGG‑AAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGc                                                                  <  1:99862/133‑1 (MQ=255)                                       aaCCCTCGCCCGCCAGGCCCGTGACCCTGAAGAGCCACGCAACAAGGCCCTGCTGGCG‑CACCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGc                                                                  <  1:175222/135‑1 (MQ=255)                                           cTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGTG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCagag                                                              >  7:282731/1‑135 (MQ=255)                                           cTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGCCCGGGCCCGTGAGCCTGCagag                                                              >  6:313197/1‑135 (MQ=255)                                           cTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCagag                                                              >  7:143417/1‑135 (MQ=255)                                                ccgccAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGt                                                         >  3:495940/1‑135 (MQ=255)                                                 cccccggCCCGTCAAGCTGAACCGCCCCGCAAGCCGGCCCGGCTGGCG‑CAGCAGGACGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTg                                                        <  6:146543/130‑1 (MQ=255)                                                  gccAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCGGCCGCGCCTGtct                                                       >  1:269968/1‑133 (MQ=255)                                                      ggCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAcc                                                   >  2:283882/1‑135 (MQ=255)                                                               aGCTGAAGAGGCCCGAATGCAGGCCCGGC‑GGCGTCCGCAGTAAGC‑TTAAAACCGCCGCCAGCAGGAAGGGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaaa                                          <  6:224465/135‑1 (MQ=255)                                                               aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaaa                                          >  8:192579/1‑135 (MQ=255)                                                                    aaGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGACCCGCCGAAAAAGGccc                                     >  4:467494/1‑135 (MQ=255)                                                                            cGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTaccacc                             >  8:83629/1‑135 (MQ=255)                                                                            cGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTaccacc                             >  4:248498/1‑135 (MQ=255)                                                                              cAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCt                           >  5:267640/1‑135 (MQ=255)                                                                              cAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCt                           >  8:137745/1‑135 (MQ=255)                                                                               aagaagGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGGGCGCCTGCAGCGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTc                          >  6:319733/1‑135 (MQ=255)                                                                                  acgccccTGCGGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCGGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCggg                       <  7:41945/131‑1 (MQ=255)                                                                                   agGCCCTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGc                      <  7:192579/135‑1 (MQ=255)                                                                                       ccTGCTGGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGAt                  >  7:333327/1‑135 (MQ=255)                                                                                         tgctgGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCCCCCTCGGGCCGATgg                >  7:54850/1‑135 (MQ=255)                                                                                          gctgGCG‑CCGCAGTACCCGG‑AAACACGCCGCCAGCAGGAAGCGCGGGCCGCCGCCGCTGCCCGCGACCGGGCCCATGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGt               <  2:425861/135‑1 (MQ=255)                                                                                          gctgGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGt               >  5:460576/1‑135 (MQ=255)                                                                                            tgGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGCGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGtgt             >  7:484688/1‑133 (MQ=255)                                                                                            tgGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGttt             >  8:135091/1‑135 (MQ=255)                                                                                            tgGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGttt             >  2:290015/1‑135 (MQ=255)                                                                                            tgGCG‑CAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCAGCCGCAGCAGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGCACCGCCGAAAAAGGCCCGTCACGCCCTCGGCCCGATGGttt             >  2:491057/1‑135 (MQ=255)                                                                                              gcg‑cAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGCCCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGttt             >  2:96966/1‑133 (MQ=255)                                                                                              gcg‑cAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGttt             >  7:515111/1‑133 (MQ=255)                                                                                               cg‑cAGGAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCa          <  5:228461/135‑1 (MQ=255)                                                                                                    gcagGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGCGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGCGGGTTTCCAGCGa      >  1:492843/1‑135 (MQ=255)                                                                                                    gcagGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGa      >  6:22775/1‑135 (MQ=255)                                                                                                      agGAAGCGG‑AAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCGGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATg    <  4:95935/135‑1 (MQ=255)                                                                                                        gAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGgc  >  5:157929/1‑135 (MQ=255)                                                                                                         aaGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGgc  <  7:137745/134‑1 (MQ=255)                                                                                                               |                                                                                                                                 CAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCG‑CAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGC  >  ACB122/5755946‑5756183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑