| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 6,038,658 | 0 | C | A | 20.0% | 136.2 / 15.2 | 85 | A37S (GCC→TCC) | puuD_3 | Gamma‑glutamyl‑gamma‑aminobutyrate hydrolasePuuD |
| Reads supporting (aligned to +/- strand): ref base C (28/40); new base A (0/17); total (28/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.86e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACACTGGGGGCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTAC > ACB122/6038522‑6038789 | acacTGGGGGCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGc > 8:1992/1‑137 (MQ=255)acacTGGGGGCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGc > 5:215260/1‑137 (MQ=255) acTGGGGGCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCaa > 2:87962/1‑137 (MQ=255) ggCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑gccgg > 5:217347/1‑137 (MQ=255) gcgcCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑gccggc > 8:270558/1‑137 (MQ=255) gcgcCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑gccggc > 5:253858/1‑137 (MQ=255) gcgcCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑gccggc > 2:322061/1‑137 (MQ=255) gTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCgc > 7:279031/1‑136 (MQ=255) gTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGa > 5:127569/1‑137 (MQ=255) atgCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACt > 2:82928/1‑137 (MQ=255) gggCTCTATATTTGACGGCGAGCCGGAAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGGGTGGCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGt < 6:215260/136‑1 (MQ=255) cGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCCAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGt > 1:108602/1‑137 (MQ=255) cGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGt > 8:256823/1‑137 (MQ=255) cGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGt > 7:114482/1‑137 (MQ=255) cTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGc > 1:358685/1‑137 (MQ=255) cTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGc > 8:338705/1‑137 (MQ=255) cGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCg > 4:128072/1‑137 (MQ=255) cGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCg > 5:147261/1‑137 (MQ=255) ttaaGGCGATCCGTTAAAAACAATGCCGTGCTGGGGAGCCTGCAGCGCTTGGGTGGCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCgtgt < 7:277737/135‑1 (MQ=255) tGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCTAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCgtgt < 5:157151/137‑1 (MQ=255) gcgcgcCGGTAAAAACAATGCCGGGCAGGCGAGCCAGCCGCGCTGGCGTGGCGCTGCCG‑CCATCAGGGGCCGGCAAGATCAGTGGCACGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCgag < 1:87962/137‑1 (MQ=255) gCGAGTCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCCGGGCTTGCGGGGCGCGGCCG‑CCATCACGGGCCGGCAAGAGCAGTGGCAACCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCgag < 2:303495/137‑1 (MQ=255) gCGAGCCGGTAAAAACAATGCCGTGCAGGCTCGCCAGCAGCGCTTGGGTGTCGCTGCCG‑CCATCCGGGGCCGGAAAGATCTGTGGCACGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCgag < 4:91526/137‑1 (MQ=255) gCGAGCCGGAAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGGGGCGCGGCCG‑CCATCACGGGCCGGCAAGATCAGTGCCACGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCgag < 4:187203/137‑1 (MQ=255) gAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGCCCGTGTGCgaggc > 3:11175/1‑136 (MQ=255) gAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGCCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCgagga < 1:107226/137‑1 (MQ=255) gCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGt > 3:134840/1‑137 (MQ=255) cGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGttg > 5:291584/1‑136 (MQ=255) cGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGttc > 2:254874/1‑137 (MQ=255) cGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGttc > 7:41855/1‑137 (MQ=255) aaggccgggccggCGCGCCAGCCGCGCTGGCGTGGGGC‑GCCGCCCAGCCGGGGCCGTAAATATCTGTGCCACCCCGCAAAC‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACct < 4:11175/134‑1 (MQ=255) cGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGa > 6:296218/1‑137 (MQ=255) cGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGa > 7:26348/1‑137 (MQ=255) cGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGa > 1:246759/1‑137 (MQ=255) gTGCAGGGGAGCCAGCAGCGCTTGCGGGGGGCTGCCG‑CCATCAGGGGCCGGAAAGATCAGTGGCATGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGAc < 7:1992/137‑1 (MQ=255) gTGCAGGCGAGCCAGCTGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAATAGCAGGGCCACGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGAc < 1:111929/137‑1 (MQ=255) tGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg < 7:338705/137‑1 (MQ=255) ggacggCGAGCAAGCAGCGTTTGCGTGGGGCGGCCG‑CCATCCGGGGCCGGTAAAATCCGTGGCAAGCGGGAAAT‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg < 2:101419/133‑1 (MQ=255) ggCAGGGGAGCCAGCAGCGCTTGCGTGTCGCGGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg < 2:106325/136‑1 (MQ=255) gcccggCGAGCCTGCAGCGCTGGGGGGTCGCGGCCG‑CCATCACGGGCCGGCAATAGCCGTGGCACGCCGCCAAT‑GCGGCCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg < 2:258755/133‑1 (MQ=255) aGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGgcgc > 6:357531/1‑137 (MQ=255) ccAGCAGCGCTTGCGTGGCGCGGCCG‑CCAGCACGGGCCGGCAAGATCAGTGGCAACCCGGCAAA‑CCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTg < 5:99060/137‑1 (MQ=255) gcagcGCTTGGGTGTCGCTGCCG‑CCATCACGGGCCGGCAATATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGaca < 1:253181/137‑1 (MQ=255) gcTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGAAAGAGCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGAt < 3:138153/137‑1 (MQ=255) gCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCaaa > 3:168057/1‑137 (MQ=255) gCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCaaa > 2:212056/1‑137 (MQ=255) gtgtCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAgg < 2:246759/137‑1 (MQ=255) cg‑ccATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCAttt < 8:82435/137‑1 (MQ=255) ttCACGGGCCGGCAATATCAGTGGCATCCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 5:347150/136‑1 (MQ=255) ttCACGGGCCGCCTTGATCTGTGGCACGCCGGAAAA‑GCCGCCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 3:290120/136‑1 (MQ=255) atccggggCGGGAAAGATCAGTGGCACGCCGGCAAT‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 1:125693/132‑1 (MQ=255) agccggggCGGGAAAGAGCTGTGAAAACCCGCAAAA‑CCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 3:189994/132‑1 (MQ=255) aacccGGGCCGGAAAGATCTGTGCCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 2:358685/133‑1 (MQ=255) aTCCCGGGCCGGCAAGATCAGGGCCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 2:149560/137‑1 (MQ=255) aTCAGGGGCCGGCATTATCCGTGGAATGCCGGCAAA‑CCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATAAAAGGGACCGCATTTgcgc < 6:218700/137‑1 (MQ=255) aTCAGGGGCCGGCAAGATCAGTGGCAAGCGGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 4:110919/137‑1 (MQ=255) aTCAGGGGCCGGCAAGATAAGGGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 6:190921/137‑1 (MQ=255) aTCAGGGGCCGGAAATATCTG‑GGCAACCCCGCAAACGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 5:47284/137‑1 (MQ=255) aTCAGGGGCCGGAAAGATCATTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 5:64797/137‑1 (MQ=255) aTCACGGGCGGGCAAGTTCAGTGGCATGCCGGAAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACGGTGTGCGAGGAGTTCTTCGCTACGTGCTGACGGCTGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 5:245323/137‑1 (MQ=255) aTCACGGGCGGGCAAGATCAGGGCCAAGCGGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 3:124532/137‑1 (MQ=255) aTCACGGGCCGGCATTATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 2:110804/137‑1 (MQ=255) aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 1:212056/137‑1 (MQ=255) aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 5:357531/137‑1 (MQ=255) aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 6:253858/137‑1 (MQ=255) aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 6:147261/137‑1 (MQ=255) aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 1:82928/137‑1 (MQ=255) aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 7:256823/137‑1 (MQ=255) aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 7:276145/137‑1 (MQ=255) aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 7:279723/137‑1 (MQ=255) aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGACGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTTACAAATATCAAAGGGACCGCATTTgcgc < 5:296218/137‑1 (MQ=255) aTCACGGGCCGGCAAGATCAGTGCCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 2:113702/137‑1 (MQ=255) aTCACGGGCCGGCAAGATCAGTGCCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 7:337505/137‑1 (MQ=255) aTCACGGGCCGGAAATATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 4:114731/137‑1 (MQ=255) aTCACGGGCCGGAAATATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 4:123781/137‑1 (MQ=255) aTCACGGGCCGGAAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 8:94127/137‑1 (MQ=255) ggCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATgg > 1:251884/1‑137 (MQ=255) cGGCAAGAGCCGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTGCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCt < 3:229377/137‑1 (MQ=255) ggCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCtt < 8:114482/137‑1 (MQ=255) gccAAGATCAGTGGCAAGCGGGAAAC‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCtt < 1:254874/135‑1 (MQ=255) cAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTac < 4:168057/137‑1 (MQ=255) aaGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTaca < 5:341910/137‑1 (MQ=255) tCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgt < 1:76418/137‑1 (MQ=255) cAAGCCGGCAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAgg < 6:316801/137‑1 (MQ=255) cAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAgg > 7:338995/1‑137 (MQ=255) cAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAgg < 7:168643/137‑1 (MQ=255) gCCGGAAAT‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTAc < 5:159943/137‑1 (MQ=255) | ACACTGGGGGCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCG‑CCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTAC > ACB122/6038522‑6038789 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |