Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,978,6000TA37.8% 44.5 / 10.9 55L684Q (CTG→CAG) sasA_19Adaptive‑response sensory‑kinase SasA
Reads supporting (aligned to +/- strand):  ref base T (9/19);  new base A (17/0);  total (34/21)
Fisher's exact test for biased strand distribution p-value = 4.00e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AAAGCCTTGATTGGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAG  >  ACB143/5978467‑5978731
                                                                                                                                     |                                                                                                                                    
aaaGCCTTGATTGGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTgg                                                                                                                                    <  4:317175/136‑1 (MQ=255)
 aaGCCTTGATTGGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTgg                                                                                                                                    <  6:267197/135‑1 (MQ=255)
      ttGATTGGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATg                                                                                                                               <  8:109193/135‑1 (MQ=255)
      ttGATTGGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATg                                                                                                                               <  7:83909/135‑1 (MQ=255)
      ttGATTGGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATg                                                                                                                               <  8:122729/135‑1 (MQ=255)
        gATTGGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCCGGcccagcg                                                                                                                             >  5:30437/1‑130 (MQ=255)
        gATTGGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCCGGGCCTgcgc                                                                                                                            >  4:350236/1‑136 (MQ=255)
        gATTGGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCATGCCCTgcc                                                                                                                             >  5:80073/1‑134 (MQ=255)
          ttGGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGCCAgccgcc                                                                                                                           >  5:123587/1‑135 (MQ=255)
                 tGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCGGGCCCGGCGCCAAGC‑ATg                                                                                                                   >  5:15935/1‑135 (MQ=255)
                      tCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGCCATGCGCCCGGC‑TTGGCCAg                                                                                                              >  5:86131/1‑135 (MQ=255)
                         tGCAACCGGGCAAGAAGGGCTTGGGCTTCACTCTTGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCTTGCGCCAGGC‑TTGGCCAGGGc                                                                                                           <  8:296905/135‑1 (MQ=255)
                                     aGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGCCATGCGCCACGC‑TTTGCCAGGGCGCACCCACCAgg                                                                                               >  6:200921/1‑135 (MQ=255)
                                            cTTGGGCTTCACTCATGACCGGGGGATGGAGGCCGGTTATGCAGTCACGCCCGGGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGC‑TTGGCCAGGGGGAACGCACGAGGTgttgtt                                                                                        <  2:4581/135‑1 (MQ=255)
                                               gggCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCGGGCCCAGCGCCCGGC‑TTGGGCCGGGCGCACGCCCGAGGTGTTGTTGGAg                                                                                    >  4:194693/1‑136 (MQ=255)
                                               gggCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGCCAATCGCCACGG‑ATGGGCCCGCCGCACGCCCGAGGTGTTGTTCgc                                                                                     >  8:7420/1‑134 (MQ=255)
                                                     cACTCTTGCCGGGGTGTGGGCGCCCGGTTATCCACCCCCGCCCGTGCACCAGCGCGCCGGGCGGCATGCCCTTAGCGCCGTGCCCAGGCGCCGGGG‑GTGCCCTGGGCGAACGCACGAGGTGTTGTTCGAGCTCgcc                                                                              <  4:114792/136‑1 (MQ=255)
                                                        tCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGGCCAGGGCAATCGCCCGGC‑TTGGGCAGGGCGCACGCCCGAGGTGTTGTTGGCGCGAgccag                                                                            >  5:135736/1‑135 (MQ=255)
                                                        tCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCCGGCCAAGCCCCCCGC‑GTGGCCAGGGCGCACCCACCAGGTGGTGTTCGAGCTAgcccgc                                                                           >  4:117479/1‑136 (MQ=255)
                                                        tCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGGCATGCGGCCTCC‑ATGGCCACGGCGCAAGCCAGAGGTGTTTTTGCAGCTCgccag                                                                            >  2:172603/1‑135 (MQ=255)
                                                        tCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTACACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGC‑TTGGCCAGGGCGAACGCACGCGGTGTTGTTCGAGCTCgcccg                                                                            >  8:73562/1‑133 (MQ=255)
                                                         cATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGCCCAGCGCCCCGC‑GTGGCCCGGGCGCCCGCACCACGTGGTGTTCGAGCAAgccagc                                                                           >  2:9355/1‑135 (MQ=255)
                                                         cATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGCCATGCGCCAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTAGAGCTCgcccgc                                                                           >  1:46114/1‑135 (MQ=255)
                                                             aCCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCATGCCCTGCGCCCCGCGATGCCCCGGCCCAAC‑CCCCAGGTGTTTTTAGAGCTCGGcagccagc                                                                       >  3:114195/1‑135 (MQ=255)
                                                              ccGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGCCCAGCCCCACGC‑ATGGCCCGGGCGAACCCACGCGGTGGTGGTAGAGCTCgccagccacc                                                                       >  8:155362/1‑132 (MQ=255)
                                                               cGGGGGTTGGCGGCCGATTATGCACTCCCGCCCGGGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCTGGC‑TTGGCCCGGGGGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTAc                                                                    <  4:167979/136‑1 (MQ=255)
                                                                    gATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCCTGCGCCAGGC‑TTGGCCAGGGCGCACGCCCGAGGTGTTGTTCGAGCTCGCCAGCCAGCGACAAcg                                                                >  1:66640/1‑135 (MQ=255)
                                                                    gATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCCGGC‑TTTGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCGACAAcg                                                                >  7:278274/1‑135 (MQ=255)
                                                                     aTGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCt                                                                                  <  2:281258/116‑1 (MQ=255)
                                                                     aTGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGGCAATCGCCAGGC‑TTGGCCATGGCGAACCCCCGAGGTGTGTTTCGTGCTCGCCAGCCCCCGACAAcgc                                                               >  1:303931/1‑135 (MQ=255)
                                                                     aTGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGCCCTGCGCCAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCt                                                                                  >  1:281258/1‑116 (MQ=255)
                                                                      tGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAAcgcg                                                              <  8:13687/135‑1 (MQ=255)
                                                                      tGGAGGCCGCTTAGTCACTCACGCCCGTGCACCCGCGCGCCGCGGTGCATGCACGTAGCGCCCTGGCAATGCGCCAGGC‑TGGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAAcgcg                                                              <  4:228098/135‑1 (MQ=255)
                                                                        gAGGCCGCTTATCCACTCCCGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACgcgc                                                             <  1:126202/134‑1 (MQ=255)
                                                                         aGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGGCATGCGCCACGC‑GTGGGCAGGGCGCACGCACCAGGTGTTGTTCGCGCTCGCCCGCCCGCGACAACCCGccc                                                           >  2:260354/1‑134 (MQ=255)
                                                                         aGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGC‑TTGGCCAGGGCGCACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGccc                                                           >  8:217933/1‑134 (MQ=255)
                                                                            ccGCTCATGCACACCCCCCGGTGCCCCCGCCCGCCTCGCTGCATCCAGTTCTCGCCCGGGCCATGCGCCCGGC‑TGGGCCGGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGccc                                                       <  4:63693/136‑1 (MQ=255)
                                                                              gCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGGCCGGGGCCAGCGCCCCGC‑GTGGGCAGGGCGCACGCACGAGGGGTTGTTGGGGCTCGGCCGCCCCCGACAACGCGCCACccccc                                                     >  3:211505/1‑135 (MQ=255)
                                                                                        tCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGCCCTGCGCCACGG‑GTGGGCCGGGCGGAAGCACGAGGTGTTTTTAGCGCTCGCCAGCCAGCTACAACGCCCCAGCCCCTGCGGGgtt                                             >  1:33652/1‑134 (MQ=255)
                                                                                          aCGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCCGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCGGCGGGGTTGcc                                          >  1:271333/1‑134 (MQ=255)
                                                                                          aCGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGCCAAGCGCCCCGC‑TTTGGCAGGGCGACCCCACCAGGTGGTGTTAGGGGTAGGCAGCCAGCTAAAACGCGCCACCCCCGTCGGGttttga                                          >  2:140642/1‑132 (MQ=255)
                                                                                            ccccGTCCACCCGCGCGCCCCGCTGCATGCACGTAGCGCCCGGCCCAGGCGCCAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGc                                        <  1:361821/134‑1 (MQ=255)
                                                                                             cccGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCCTGCGCCAGGC‑GTGGCCAGGGCGAACGCACGAGGTGGTGTTAGAGCTCGCCCGCCCGCTACAACGCGCCCGCCCCGGCGGGTTTGCCGc                                        >  2:93653/1‑134 (MQ=255)
                                                                                             cccGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTGGAGCTCGCCAGCCCGCGACAACGCGCCAGCCCCGGCGGGTTTGCAGCg                                       >  2:314969/1‑135 (MQ=255)
                                                                                             cccGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCg                                       <  6:96683/135‑1 (MQ=255)
                                                                                             cccGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGCCCTGCGCCAGGG‑TTGGGCAGGGCGGACGCACGAGGTGTTGTTGGAGCGCGCCCGCCCGCGTCAACGCGCCAGCCCCAGCGGGTTTGGAGCg                                       >  5:172470/1‑135 (MQ=255)
                                                                                              ccGTGCACCAGCGCGCCTCGCTGCATGCAGGTATCGCCCTGCCCATGCGCCAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCgg                                      <  7:165044/135‑1 (MQ=255)
                                                                                                    aCCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGC‑TTTGCCCGGGGGCACGCACGAGGGGTTGTTCGAGCTAGCCCGCCCGCGAACACGCCCCAGCCCCGGGGGGGTTGCCGAGGATGACt                                >  2:107051/1‑135 (MQ=255)
                                                                                                       ggcgcgcCGCGCTGCATCCACGTAAGCCCCTGGCCAGGCGCCCGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCg                             <  1:358950/134‑1 (MQ=255)
                                                                                                       cgcgcccCTCGCTGCATCAACTTAGCGCCCTGGCCTTGCGCCAGTC‑TGGGCCCGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCg                             <  7:164457/134‑1 (MQ=255)
                                                                                                           cgcCTCGCTGCATGCACGTATCGCCCGGGCCCTGCGCCCGGC‑GTGGCCCGGGCGCACGCCCGAGGTGGTGTTGGCGCTCGCCCGCCCGCGACACCGCGCCCCCCCCGGGGGGGTTGGAGCGGGTGTCTGCGAAGc                         >  6:315805/1‑135 (MQ=255)
                                                                                                           cgcCTCGCTGCATGCACGTATCGCCCGGGCCATGCGCCCGGC‑GTGGCCAGGGCGCACGCACGCGGGGGTTTTCGCGCTCGCCCGCCCGCTACAACGCGCCCGCCCCGGCGAGTTTGCAGCGGATGACTGGGAAGc                         >  8:91591/1‑135 (MQ=255)
                                                                                                              cTCGCTGCATGCACGTATCGCCCAGGGCAAGCGCCCGGC‑GTGGCCAGGGGGCACGCACGAGGGGGTTTTGGAGCGCGCCAGCCCGCTTCAACGCGCCCGCCCCAGCGGGGTTGGCGCGGCTGACAGGGGAGCggt                      >  2:327272/1‑133 (MQ=255)
                                                                                                                  cTGCATGCACGTATCGCCCTGGCCATGCGCAAGGC‑TTGGCCTGGGGGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTg                  <  5:78983/135‑1 (MQ=255)
                                                                                                                                  cccTGCCCATGCGCCGGGC‑GGGGCCTGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAg  <  6:251284/135‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                    
AAAGCCTTGATTGGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAG  >  ACB143/5978467‑5978731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑