| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB143 | 690,144 | A→G | intergenic (+21/‑2438) | bauC → / → PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 690,144 | 0 | A | G | 91.7% | 13.5 / ‑6.3 | 12 | intergenic (+21/‑2438) | bauC/PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (11/0); minor base T (1/0); total (12/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGC‑CGTCA‑GGC‑CTCCCCGC‑TTTG‑TTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTT > ACB143/690028‑690248 | ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGC‑CGGGA‑GGC‑CGccccc > 7:196440/1‑134 (MQ=255) aCCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGG‑CGGGC‑GGC‑CGCCCCCG‑GGTG‑TTTTTtgggg > 7:79253/1‑135 (MQ=255) aCCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGG‑CCGGA‑GGC‑CACCCCCCGGTTG‑TTTTTgtggg > 3:133073/1‑136 (MQ=255) aCCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCGGGACA‑GGC‑GACCCCCG‑GTTT‑TTTTTggggg > 3:133081/1‑136 (MQ=255) ccGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGTGGG‑CGGGACGGC‑CGCCCGCC‑TTTT‑TTTTTGTGggt > 6:69236/1‑135 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGG‑CGGCG‑GGC‑AGCCCCCG‑GTTG‑TTTTTggggggggggg > 8:180749/1‑129 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGC‑CGGCC‑CGC‑CCCCCCCG‑GTGG‑TTTTTTGGggtgggtt > 6:187361/1‑135 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGG‑CGTCC‑AGG‑CGCGCCGC‑GGTG‑TTTTTTGGgggtggtgg > 8:289273/1‑133 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGAAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGC‑GGGCC‑GGC‑GCCCCCCC‑TTGT‑TTTTTTGGggttggtt > 7:102981/1‑135 (MQ=255) ccGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGG‑CGGGA‑GGC‑CGACCCCC‑GGTGTTTTTTGGGGGTGGTTTGTTG‑TGTTTGGGGGGGGCTGttt > 7:195014/1‑135 (MQ=255) cAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGC‑CGCCC‑GCCGCCCCCCGT‑TTTT‑TTTTTGGGGGT‑‑GTTGTTGGTGGTTGGGGGGGACGGTTTTTTTAGGGGGTTTGGTGTGGGTtgtt > 2:277581/1‑133 (MQ=255) tAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGG‑CGGCA‑GGG‑CGCCCCGC‑CGTG‑TTTTTTGGGGGTGGTTGTTGGTGTTGGGGGGGGACTGTTTTTATATGGTGTTGGGGGTGAGTTGTGTTCGCCCCCAcccga > 8:358945/1‑133 (MQ=255) | GGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGC‑CGTCA‑GGC‑CTCCCCGC‑TTTG‑TTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTT > ACB143/690028‑690248 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |