Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	5,386,500	C→A	intergenic (‑509/+1142)	CJ019_05070 ← / ← nptII	hypothetical protein/neomycin phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,386,500	0	C	A	100.0%	34.3 / NA	11	intergenic (‑509/+1142)	CJ019_05070/nptII	hypothetical protein/neomycin phosphotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (7/4);  total (7/4)

TAGCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGTCGT‑‑‑CA‑GG‑‑ATC‑‑ATCC‑GGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGG  >  ACB143/5386369‑5386605                                                                                                                                    |                                                                                                                   tAGCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagtggt                                                                                                               <  3:302772/137‑1 (MQ=255) tAGCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagtggt                                                                                                               <  7:234805/137‑1 (MQ=255)  aGCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagtggtg                                                                                                              <  2:87913/137‑2 (MQ=255)  aGCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagtggtg                                                                                                              >  3:102125/1‑136 (MQ=255)  aGCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagtggtg                                                                                                              <  4:206708/137‑2 (MQ=255)    ccAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagtggtgtt                                                                                                            >  1:338404/1‑134 (MQ=255)    ccAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagtggtgta                                                                                                            >  6:77941/1‑134 (MQ=255)    ccAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGAAGACGGCGGTCTCagtggtgta                                                                                                            >  2:120210/1‑134 (MQ=255)                                                           aaaaaTTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCC‑CGACGCTAGCATCGCATGAGAAAGCCCCCGGAAGATCACCTTc                                                >  2:277347/1‑137 (MQ=255)                                                                     cGAATTCTTTCATTTAAGACCCTAATATGACATCTCGCCCGGCTTCTCGACGACGGCGGTCTCAGAGGTGTACG‑GT‑‑ACA‑‑AACCACGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttt                                      >  3:111579/1‑137 (MQ=255)                                                                         ttCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTa                                  <  7:60037/137‑1 (MQ=255)                                                                                      caCCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGt                     <  6:12099/136‑1 (MQ=255)                                                                                            aaTATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGGTAAAA‑‑ACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCatg               <  5:293054/137‑3 (MQ=255)                                                                                            aaTATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg               <  3:94555/137‑1 (MQ=255)                                                                                               atGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGTGCTTTTTTATTGCGCTGCGTGTGCAAGGGCg            >  4:192268/1‑137 (MQ=255)                                                                                               atGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCg            >  2:66454/1‑137 (MQ=255)                                                                                                      ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt     >  8:236801/1‑137 (MQ=255)                                                                                                      ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCC‑AGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt     >  5:357066/1‑137 (MQ=255)                                                                                                         ggccggcTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTggg  >  1:121920/1‑137 (MQ=255)                                                                                                                                    |                                                                                                                   TAGCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGTCGT‑‑‑CA‑GG‑‑ATC‑‑ATCC‑GGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGG  >  ACB143/5386369‑5386605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑